Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,027,191 A→C 9.6% H252Q (CAT→CAG vraS ← sensor histidine kinase VraS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,027,1910AC 9.6% 175.8 / 5.9 70H252Q (CAT→CAGvraSsensor histidine kinase VraS
Reads supporting (aligned to +/- strand):  ref base A (33/29);  new base C (4/3);  total (37/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCATCAACATTAAAACCTTTACCATTATCTTGAATTCTCAACAATAAATAATCGTCTTTATTAAACAATTCTACTGTCACTTTTGTACCGTTTGAATGACGCAATGTATTCGAAATTGCTTCCTGTGTAATTCTGAACAAATGATCTTCAATACCTTTAGGCACTTTAAAATCTTGTATTTCATGCACAACTTTCATTGGCACTTTTTTTTGTAAATCAATAACTAAATCTTTAATACCCTCACCTAAAGATTTGTCTTTTAAACCAAGCGGTCTTA  >  CP000730/2027051‑2027327
                                                                                                                                            |                                                                                                                                          
tcatcaACATTAAAACCTTTACCATTATCTTGAATTCTCAACAATAAATAATCGTCTTTATTAAACAATTCTACTGTCACTTTTGTACCGTTTGAATGACGCAATGTATTCGAAATTGCTTCCTGTGTAATTCTGAACaaa                                                                                                                                            <  2:591102/141‑1 (MQ=255)
 catcaACATTAAAACCTTTACCATTATCTTGAATTCTCAACAATAAATAATCGTCTTTATTAAACAATTCTACTGTCACTTTTGTACCGTTTGAATGACGCAATGTATTCGAAATTGCTTCCTGTGTAATTCTGAACAAAt                                                                                                                                           >  1:496877/1‑141 (MQ=255)
    aaaCATTAAAACCTTTACCATTATCTTGAATTCTCAACAATAAATAATCGTCTTTATTAAACAATTCTACTGTCACTTTTGTACCGTTTGAATGACGCAATGTATTCGAAATTGCTTCCTGTGTAATTCTGAACAAATGAt                                                                                                                                        <  2:194468/140‑1 (MQ=255)
     aaCATTAAAACCTTTACCATTATCTTGAATTCTCAACAATAAATAATCGTCTTTATTAAACAATTCTACTGTCACTTTTGTACCGTTTGAATGACGCAATGTATTCGAAATTGCTTCCTGTGTAATTCTGAACAAATGATc                                                                                                                                       <  1:254614/141‑1 (MQ=255)
       cATTAAAACCTTTACCATTATGTTGAATTCTCAACAATAAATACTCGTCTTTATTAAACAATTCTACTGTCACCTGTGTACTGTTTGTATGTCGCAATGTATTCGTAATTGCTTCCTGTTTGATTCTTAGCAAATGATCtt                                                                                                                                     >  1:478929/1‑141 (MQ=255)
       cATTAAAACCTTTACCATTATCTTGAATTCTCAACAATAAATAATCGTCTTTATTAAACAATTCTACTGTCACTTTTGTACCGTTTGAATGACGCAATGTATTCGAAATTGCTTCCTGTGTAATTCTGAACAAATGATCtt                                                                                                                                     <  1:151534/141‑1 (MQ=255)
           aaaaCCTTTACCATTATCTTGAATTCTCAACAATAAATAATCGTCTTTATTAAACAATTCTACTGTCACTTTTGTACCGTTTGAATGACGCAATGTATTCGAAATTGCTTCCTGTGTAATTCTGGACAAATGTTCTTCAAt                                                                                                                                 >  2:83699/1‑141 (MQ=255)
           aaaaCCTTTACCATTATCTTGAATTCTCAACAATAAATAATCGTCTTTATTAAACAATTCTACTGTCACTTTTGTACCGTTTGAATGACGCAATGTATTCGAAATTGCTTCCTGTGTAATTCTGAACAAATGATCTTCAAt                                                                                                                                 >  2:369268/1‑141 (MQ=255)
               ccTTTACCATTATCTTGAATTCTCAACAATAAATAATCGTCTTTATTAAACAATTCTACTGTCACTTTTGTACCGTTTGAATGACGCAATGTATTCGAAATTGCTTCCTGTGTAATTCTGAACAAATGATCTTCAATACCt                                                                                                                             >  2:374284/1‑141 (MQ=255)
                  ttACCATTATCTTGAATTCTCAACAATAAATAATCGTCTTTATTAAACAATTCTACTGTCACTTTTGTACCGTTTGAATGACGCAATGTATTCGAAATTGCTTCCTGTGTAATTCTGAACAAATGATCTTCAATACCTTTa                                                                                                                          >  1:435840/1‑141 (MQ=255)
                       aTTATCTTGAATTCTCAACAATAAATAATCGTCTTTATTAAACAATTCTACTGTCACTTTTGTACCGTTTGAATGACGCAATGTATTCGAAATTGCTTCCTGTGTAATTCTGAACAACTGATCTTCAATACCTTTAGGCAc                                                                                                                     <  1:497375/141‑1 (MQ=255)
                         tATCCTGAATTCTCAACAATAAATAATCGTCTTTATTAAACAATTCTACTGTCACTTTTGTACCGTTTGAATGACGCAATGTATTCGAAATTGCTTCCTGTGTAATTCTGAACAAATGATCTTCAATACCTTTAGGCACtt                                                                                                                   <  1:139588/141‑1 (MQ=255)
                          aTCTTGAATTCTCAACAATAAATAATCGTCTTTATTAAACAATTCTACTGTCACTTTTGTACCGTTTGAATGACGCAATGTATTCGAAATTGCTTCCTGTGTAATTCTGAACAAATGATCTTCAATACCTTTAGGCACttt                                                                                                                  <  1:94935/141‑1 (MQ=255)
                              tGAATTCTCAACAATAAATAATCGTCTTTATTAAACAATTCTACTGTCACTTTTGTACCGTTTGAATGACGCAATGTATTCGAAATTGCTTCCTGTGTAATTCTGAACAACTGATCTTCAATACCTTTAGGCACTTTaaaa                                                                                                              >  1:373514/1‑141 (MQ=255)
                                   tctcAACAATAAATAATCGTCTTGATTAAACAATTCTACTGTCACTTGTGTACCGTTTGAATGACGCAATGTATTCGAAATTGCTTCCTGTGTAATTCTGAACAAATGATCTTCAATACCTTGAGGCACTTTAAAATCTTg                                                                                                         <  2:340055/141‑1 (MQ=255)
                                    ctcAACAATAAATAATCGTCTTTATTAAACAATTCTACTGTCACTTTTGTACCGTTTGAATGACGCAATGTATTCGAAATTGCTTCCTGTGTAATTCTGAACAACTGATCTTGAATACCTTTAGGCACTTTAAAATCTTGt                                                                                                        >  1:119706/1‑141 (MQ=255)
                                    ctcAACAATAAATAATCGTCTTTATTAAACAATTCTACTGTCACTTTTGTACCGTTTGAATGACGCAATGTATTCGAAATTGCTTCCTGTGTAATTCTGAACAAATGATCTTCAATACCTTTAGGCACTTTAAAATCTTGt                                                                                                        <  2:424702/141‑1 (MQ=255)
                                      caacaaTAAATAATCGTCTTTATTAAACAATTCTACTGTCACTTTTGTACCGTTTGAATGACGCAATGTATTCGAAATTGCTTCCTGTGTAATTCTGAACAAATGATCTTCAATACCTTTAGGCACTTTAAAATCTTGTAt                                                                                                      <  2:372231/141‑1 (MQ=255)
                                           ataaataaTCGTCTTTATTAAACAATTCTACTGTCACTTTTGTACCGTTTGAATGACGCAATGTATTCGAAATTGCTTCCTGTGTAATTCTGAACAAATGATCTTCAATACCTTTAGGCACTTTAAAATGTTGTTTTTcct                                                                                                 >  2:229347/1‑139 (MQ=255)
                                            taaataaTCGTCTTTATTAAACAATTCTACTGTCACTTTTGTAACGTTTGAATGAAGCAATGTATTAGAAATTGATACCTGCGTATTTCTGAACAAATGAACATCAATACCTCTATGCACGTTAAAATCTTGTATTTCATg                                                                                                >  2:52620/1‑141 (MQ=255)
                                             aaataaTCGTCTTTATTAAACAATTCTACTGTCACTTTTGTACCGTTTGAATGACGCAATGTATTCGAAATTGCTTCCTGTGTAATTCTGAACAAATGATCTTCAATACCTTTAGGCACTTTACATTCTTGTATTTCATGc                                                                                               <  2:461878/141‑1 (MQ=255)
                                             aaataaTCGTCTTTATTAAACAATTCTACTGTCACTTTTGTACCGTTTGAATGACGCAATGTATTCGAAATTGCTTCCTGTGTAATTCTGAACAAATGATCTTCAATACCTTTAGGCACTTTAAAATCTTGTATTTCATGc                                                                                               >  2:451930/1‑141 (MQ=255)
                                             aaataaTCGTCTTTATTAAACAATTCTACTGTCACTTTTGTACCGTTTGAATGACGCAATGTATTCGAAATTGCTTCCTGTGTAATTCTGAACAAATGATCTTCAATACCTTTAGGCACTTTAAAATCTTGTATTTCATGc                                                                                               <  2:48122/141‑1 (MQ=255)
                                              aataatCGTCTTTATTAAACAATTCTACTGTCACTTTTGTACCGTTTGAATGACGCAATGTATTCGAAATTGCTTCCTGTGTAATTCTGAACAAATGATCTTCAATACCTTTAGGCACTTTAAAATCTTGTATTTCATGca                                                                                              >  1:409008/1‑141 (MQ=255)
                                                       cTTTATTAAACAATTCTACTGTCACTTTTGTACCGTTTGAATGACGCAATGTATTCGAAATTGCTTCCTGTGTAATTCTGAACAAATGATCTTCAATACCTTTAGGCACTTTAAAATCTTGTATTTAATGAACACCTTTCa                                                                                     >  1:342850/1‑141 (MQ=255)
                                                       cTTTATCAAACAATTCTACTGTCACTTTTGTACCGTTTGAATGACGCAATGTATTCGAAATTGCTTCCTGTGTAATTCTGAACAAATGATCTTCAATACCTTTAGGCACTTTAAAATCTTGTATTTCATGCACAACTTTCa                                                                                     >  2:337443/1‑141 (MQ=255)
                                                          tattaAACAATTCTACTGTCACTTTTGTACCGTTTGAATGACGCAATGTATTCGAAATTGCTTCCTGTGTAATTCTGAACAAATGATCTTCAATACCTTTAGCCACTTTAAAATCTTGTATTTCATGCACAACTTTCATTg                                                                                  >  2:424327/1‑141 (MQ=255)
                                                          tattaAACAATTCTACCGTCACTTTTGTACCGTTTGAATGACGCAATGTATTCGAAATTGCTTCCTGTGTAATTCTGAACAAATGATCTTCAATACCTTTAGGCACTTTAAAATCTTGTATTTCATGCACAACTTTCATTg                                                                                  >  2:122619/1‑141 (MQ=255)
                                                           attaAACAATTCTACTGTCACTTTTGTACCGTTTGAATGACGCAATGTATTCGAAATTGCTTCCTGTGTAATTCTGAACAAATGATCTTCAATACCTTTAGGCACTTTAAAATCTTGCATTTCATGCACAACTTTCATTgg                                                                                 >  2:96612/1‑141 (MQ=255)
                                                            ttaAACAATTCTACTGTCACTTTTGTACCGTTTGAATGACGCAATGTATTCGAAATTGCTTCCTGTGTAATTCTGAACAACTGATCTTCAATACCTTTAGGCACTTTAAAATCTTGTATTTCATGCACAACTTTCATTGGc                                                                                >  2:391111/1‑141 (MQ=255)
                                                            ttaAACAATTCTACTGTCACTTTTGTACCGTTTGAATGACGCAATGTATTCGAAATTGCTTCCTGTGTAATTCTGAACAAATGATCTTCAATACCTTTAGGCACTTTAAAATCTTGTATTTCATGCACAACTTTCATTGGc                                                                                >  2:263436/1‑141 (MQ=255)
                                                             taAACAATTCTACTGTCACTTTTGTACCGTTTGAATGCCGCAATGTATTCGAAATTGCTTCCTGTGTAATTCTGAACAAATGATCTTCAATACCTTTAGGCACTTTAAAATCTTGTATTTCATGCACAACTTTCATTGGCa                                                                               <  1:263436/141‑1 (MQ=255)
                                                                 cAATTCTACTGTCACTTTTGTACCGTTTGAATGACGCAATGTATTCGAAATTGCTTCCTGTGTAATTCTGAACAAATGATCTTCAATACCTTTAGGCACTTTAAAATCTTGTATTTCATGCACAACTTGCATTGGCACttt                                                                          >  2:579182/1‑141 (MQ=255)
                                                                 cAATTCTAATGTCACTTTCGCACCCTCTGAATGACGCAATGTATTCGAAATTGCTTCCTGTGTAATTCTGAACAAACGATCTTCAATACCTTTCGGCACTTTAAACTCTTGTATTTCATGCACAACTTTCATTGGCACttt                                                                          <  1:326942/141‑1 (MQ=255)
                                                                   tttCTACTGGCACTTTTTTACCGTTTGAATCACGCAATGTATTCGAAATTGCTTCCTGTGTAATTCTGAACAAATGATCTTCAATACCTTTAGGCACTTTAAAATCTTGTATTTCATGCACAACTTTCATTGGCACttttt                                                                        <  1:321293/140‑1 (MQ=255)
                                                                      cTACTGTCACTTTTGTACCGTTTGAATGACGCAATGTATTCGAAATTGCTTCCTGTGTAATTCTGAACAAATGATCTTCAATACCTTTAGGCACTTTAAAATCTTGTATTTCATGCGCAACTTTCATTGGCCCtttttttt                                                                     >  2:7504/1‑141 (MQ=255)
                                                                      cTACTGTCAATTTTGTACCGTTTGAATGACGCAATGTATTCGAAATTGCTTACTGTGTAATTCTAAACAAATGATCTTCAATACCTTTAGGCGCTTTAAAATCTTGTGTTTCATGCGCAACTTTCATTGGCCCtttttctt                                                                     >  2:109517/1‑141 (MQ=255)
                                                                       tACTGTCACTTTTGTACCGTTTGAATGACGCAATGTATTCGAAATTGCTTCCTGTGTAATTCTGAACAAATGATCTTCAATACCTTTAGGCACTTTAAAATCTTGTATTTCATGCACAACTTTCATTGGCACTTTTTTTTg                                                                    >  1:566154/1‑141 (MQ=255)
                                                                          tGTCACTTTTGTACCGTTTGAATGACGCAATGTATTCGAAATTGCTTCCTGTGTAATTCTCAACAAATGATCTTCAATACCTTTAGGCACTTTAAAATCTTGTATTTCATGCACAACTTTCATTGGCACTTTTTTTTGTaa                                                                 <  2:438076/141‑1 (MQ=255)
                                                                           ttCACTTTTGTACCGTTTGAATGACGCAATGTATTCGAAATTGCTTCCTGTGTAATTCTGAACAAATGATCTTCAATACCTTTAGGCACTTTAAAATCTTGTATTTCATGCACAACTTTCATTGGCACTTTTTTTTGTaaa                                                                <  2:484556/140‑1 (MQ=255)
                                                                           gTCACTTTTGTACCGTTTGAATGACGCAATGTATTCGAAATTGCTTCCTGTGTAATTCTGAACAAATGATCTTCAATACCTTTAGGCACTTTAAAATCTTGTATTTCATGCACAACTTTCATTGGCACTTTTTTTTGTaaa                                                                <  1:575626/141‑1 (MQ=255)
                                                                              ccTTTTGTACCGTTTGAATGACGCTATGTATTCGAAATTGCTTCCTGTGTAATTCTGAACAAATGATCTTCAATACCTTTAGGCACTTTAAAATCTTGTATTTCATGCACAACTTTCATTGGCACTTTTTTTTGTAAATCa                                                             <  2:561678/140‑1 (MQ=255)
                                                                                    gTACCGGTTGAATGACGCAATGTATTCGAAATTGCTTCCTGTGTAATTCTGAACAACTGATCTTCAATACCTTTAGGCACTTTAAAATCTTGTATTTCATGCACAACTTTCATTGGCCCTTTTTTTGTAAATCAATAACTa                                                      >  1:103332/1‑141 (MQ=255)
                                                                                       ccGTTTGAATGACGCAATGTATTCGAAATTGCTTCCTGTGTAATTCTGAACAAATGATCTTCAATACCTTTAGGCACTTTAAAACCTTGTCGCGCAGTCACAAATTTCATTGGCACTTTTTTTTGTAAATCAATAACTaaa                                                    >  1:441432/1‑141 (MQ=255)
                                                                                          tttGAATGACGCAATGTATTCGAAATTGCTTCCTGTGTAATTCTGAACAAATGATCTTCAATACCTTTAGGCACTTTAAAATCTTGTATTTCATGCACAACTTTCATTGGCACTTTTTTTTGTAAATCAATAACTAAATCt                                                 <  2:294744/141‑1 (MQ=255)
                                                                                             gAATGACGCAATGTATTCGAAATTGCTTCCTGTGTAATTCTGAACAAATGATCTTCAATACCTTTAGGCACTTTAAAATCTTGTATTTCATGCACAACTTTCATTGGCACTTTTTTTTGTAAATCAATAACTAAATCTTTa                                              >  2:542692/1‑141 (MQ=255)
                                                                                                 gACGCAATGTATTCGAAATTGCTTCCTGTGTAATTCTGAACAAATGATCTTCAATACCTTTAGGCACTTTAAAATCTTGTATTTCATGCACAACTTTCATTGGCACTTTTTTTTGTAAATCAATAACTAAATCTTTAATAc                                         <  2:344804/141‑1 (MQ=255)
                                                                                                  aCGCAATGTATTCGAAATTGCTTCCTGTGTAATTCTGAACAAATGATCTTCAATACCTTTAGGCACTTTAAAATCTTGTATTTCATGCACAACTTTCATTGGCACTTTTTTTTTTTTTTCAATAACTAAAACTTTAAAAcc                                        >  2:174746/1‑141 (MQ=255)
                                                                                                     cAATGTATTCGAAATTGCTTCCTGTGTAATTCTGAACAAATGATCTTCAATACCTTTAGGCACTTTAAAATCTTGTATTTCATGCACAACTTTCATTGGCACTTTTTTTTTTTATTTTTTAACTAAATCTTTAATACCCTc                                     >  2:544991/1‑141 (MQ=255)
                                                                                                      aaTGTATTCGAAATTGCTTCCTGTGTAATTCTGAACAAATGATCTTCAATACCTTTAGGCACTTTAAAATCTTGTATTTCATGCACAACTTTCATTGGCACTTTTTTTTGTAAATCAATAACTAAATCTTTAATACCCTCa                                    >  1:193380/1‑141 (MQ=255)
                                                                                                        tgtgTTCGAATTTGCTTCCTGTGTAATTCTGAACAAATGATCTTCAATACCTTTAGGCACTTTAAAATCTTGTATTTCATGCACAACTTTCATTGGCACCTTTTTTTGTAAATCAATAACTAAATCTTTAATACCCTCAcc                                  <  2:43416/141‑1 (MQ=255)
                                                                                                           aTTCGAAATTGCTTCCTGTGTAATTCTGAACAGATGATCTTCAATACCTTTAGGCACTTTAAAATCTTGTATTTCATGCACAACTTTCATTGGCACTTTTTTTTGTAAATCAATAACTAAATCTTTAATACCCTCACCTaa                               >  1:437459/1‑141 (MQ=255)
                                                                                                           aTTCGAAATTGCTTCCTGTGTAATTCTGAACAAATGATCTTCAATACCTTTAGGCACTTTAAAATCTTGTATTTCATGCACAACTTTCATTGGCACTTTTTTTTGTAAATCAATACCTACATCTTTAATACCCTCACCTaa                               >  2:609724/1‑141 (MQ=255)
                                                                                                           aTTCGAAATTGCTTCCTGTGTAATTCTGAACAAATGATCTTCAATACCTTTAGGCACTTTAAAATCTTGTATTTCATGCACAACTTTCATTGGCACTTTTTTTTGTAAATCAATAACTAAATCTTTAATACCCTCACCTaa                               >  1:108555/1‑141 (MQ=255)
                                                                                                           aTTCGAAATTGCTTCCTGTGTAATTCTGAACAAATGATCTTCAATACCTTTAGGCACTTTAAAATCTTGTATTTCATGCACAACTTTCATTGGCACTTTTTTTTGTAAATCAATAACTAAATCTTTAATACCCTCACCTaa                               <  2:374119/141‑1 (MQ=255)
                                                                                                               gAAATTGCTTCCTGTGTAATTCTGAACAAATGATCTTCAATACCTTTAGGCACTTTAAAATCTTGTATTTCATGCACAACTTTCATTGGCACTTTTTTTTGTAAATCAATAACTAAATCTTTAATACCCTCACCTAAAGAt                           <  1:187133/141‑1 (MQ=255)
                                                                                                                aaaTTGCTTCCTGTGTAATTCTGAACAAATGATCTTCAATACCTTTAGGCACTTTAAAATCTTGTATTTCATGCACAACTTTCATTGGCACTTTTTTTTGTAAATCAATAACTACATCTCTAACACCCTCAccctcagcct                          <  2:510813/141‑9 (MQ=255)
                                                                                                                   ttGCTTCCTGTTTAATTCTCATCAAATGAGCTTCAATACCTTTAGGCACTTTAAAATCTTGTATTGCATGGACAACTTTCATTGGCACTTTTTTTTGTAAATCAATAACTAAATCTTTAATACCCTCGCCTAAAGATTTGt                       <  2:155371/141‑1 (MQ=255)
                                                                                                                     gCTTCCTGTGTAATTCTGAACAAATGATCTTCAATACCTTTAGGCACTTTAAAATCTTGTATTTCATGCACAACTTTCATTGGCACTTTTTTTTGTAAATCAATAACTAAATCTTTAATCCCCTCACCTAAAGATTTGTCt                     >  2:498364/1‑141 (MQ=255)
                                                                                                                     gCTTCCTGTGTAATTCTGAACAAATGATCTTCAATACCTTTAGGCACTTTAAAATCTTGTATTTCATGCACAACTTTCATTGGCACTTTTTTTTGTAAATCAATAACTAAATCTTTAATACCCTCACCTAACGATTTGTCt                     >  1:108282/1‑141 (MQ=255)
                                                                                                                       ttCCTGTGTAATTCTGAACAAATGATCTTCAATACCTTTAGGCACTTTAAAATCTTGTATTTCATGCACAACTTTCATTGGCACTTTTTTTTGTAAATCAATAACTAAATCTTTAATACCCTCACCTAAAGATTTGTCttt                   >  1:211066/1‑141 (MQ=255)
                                                                                                                         ccTGTGTAAGTCTTCACAATTGATCTTCAATACCTTTAGGCACTTAAACATCTAATATATCGTTCACAGCTTTCATTGTCACTTTTTTTTGTAAATCAATAACTAAATCTTTAATACCCTCACCTAAAGACTTGTCTTTTa                 <  2:489621/141‑1 (MQ=255)
                                                                                                                          cTGTGTACTTCCGAACCACTGCTCTTTAATCCCTTTAGGCACTTTAAAATCTTGGATTTCATGCACAACTTTCATTGGCACTTTTTTTTGTAAATCAATAACTAAATCTTTAATACCCTCACCTAAAGATTTGTCTTTTaa                <  2:476190/141‑1 (MQ=255)
                                                                                                                          cTGTGTAATTCTGAACAAATGATCTTCAATACCTTTAGGCACTTTAAAATCTTGTATTTCATGCACAACTTTCATTGGCACTTTTTTTGTAAATCAATAACTAAATCTTTAATACCCTCACCTAAAGATTTGTCTTTTaaa               <  2:192744/141‑1 (MQ=255)
                                                                                                                                taTTCTGAATAAAACATTTTTAACTCCTTTAGGCAATTTAAAATCTTGTATTTAATGAACAACTTTCATTGGCACCTTTTTTTTGTAAATCAATAACTAAATCTTTACATTCCGTACCTACAGATTTGTCTTTTAAACCaa           <  2:102841/140‑1 (MQ=255)
                                                                                                                                 aTTCTGAACAACTGATCGTCAATACCTTTAGGCACTTTAAAATCTTGTATTTCATGCACCACTTTCATTGGCACTTTTTTTTGTAAATCAATAACTAAATCTTTAATACCCTCACCCAAAGCTTTGTCTTTTAAACCAAGc         <  1:314345/141‑1 (MQ=255)
                                                                                                                                   tCTGAACAAATGATCTTCAATACCTTTAGGCACTTTAAAATCTTGTATTTCATGCACAACTTTCATTGGCACTTTTTTTTGTAAATCAATAACTAAATCTTTAATACCCTCACCTAAAGATTTGTCTTTTAAACCAAGCgg       >  2:567873/1‑141 (MQ=255)
                                                                                                                                   tCTGAACAAATGATCTTCAATACCTTTAGGCACTTTAAAATCTTGTATTTCATGCACAACTTTCATTGGCACTTTTTTTTGTAAATCAATAACTAAATCTTTAATACCCTCACCTAAAGATTTGTCTTTTAAACCAAGCgg       <  1:243125/141‑1 (MQ=255)
                                                                                                                                     tGAACAAATGATCTTCAATACCTTTAGGCACTTTAAAATCTTGTATTTCATGCACAACTTTCATTGGCACTTTTTTTTGTAAATCAATAACTAAATCTTTAATACCCTCACCTAAAGATTTGTCCTTTAAACCAAGCGGTc     <  1:305092/141‑1 (MQ=255)
                                                                                                                                      aaacaacTGATCTTCAATACCTTTAGCCACTTTAAAATCTTGTATTTCATGCACAACTTTCATTGGCACTTTTTTTTGTAAATCAATAACTAAATCTTTAATACCCTCACCTAAAGATTTGTCTTTTAAACCAAGCGGTCt    <  2:119706/140‑1 (MQ=255)
                                                                                                                                        aCAAATTATCCTCAATACCTTTAGGCACTTTAAAATCTTGTATTTCATGAACACCTTTCATTGGCACTTTTTTTTGTAAATCAATAACTAAATCTTTAATACCCTCACCTAAAGATTTGTCTTTTAAACCAAGCGGTCTTa  <  1:201222/141‑1 (MQ=255)
                                                                                                                                        aCAAATGATCTTCAATACCTTTAGGCACTTTAAAATCTTGTATTTCATGCACAACTTTCATTGGCACTTTTTTTTTTTTTTTTTTATCTAAATCTTTAATACCCTCACCTAAAGTTTTGTTTTTTAAACTAAGCAGTCTTa  >  2:518982/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                          
TCATCAACATTAAAACCTTTACCATTATCTTGAATTCTCAACAATAAATAATCGTCTTTATTAAACAATTCTACTGTCACTTTTGTACCGTTTGAATGACGCAATGTATTCGAAATTGCTTCCTGTGTAATTCTGAACAAATGATCTTCAATACCTTTAGGCACTTTAAAATCTTGTATTTCATGCACAACTTTCATTGGCACTTTTTTTTGTAAATCAATAACTAAATCTTTAATACCCTCACCTAAAGATTTGTCTTTTAAACCAAGCGGTCTTA  >  CP000730/2027051‑2027327

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: