Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,124,5230TC20.4% 97.1 / 11.7 44D217D (GAT→GAC) USA300HOU_2004possible bifunctional S24 family peptidase/transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (12/23);  new base C (0/9);  total (12/32)
Fisher's exact test for biased strand distribution p-value = 4.66e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

ATGTTTAAAGATGGCGAAATCATATTTGTGGAGAAAACTCACAATATAAAGAATGGACAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGTTGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTAGACTTTGAAACTATGAATGAACATATGAACAGCCCTTGCGAAGT  >  CP000730/2124374‑2124672
                                                                                                                                                     |                                                                                                                                                     
aTGTTTAAAGATGGCGAAATCATTTTTGTGGAGAAAACTCACAATATAAAGAATGGACAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCGTTGTTGAAGTTGATAGATTGACGCTAGTTTCACTATATAAAGAtt                                                                                                                                                      <  1:480120/151‑1 (MQ=255)
 tGTTTAAAGATGGCGAAATCATATTTGTGGAGAAAACTCACAATATAAAGAATGGACAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGTTGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTa                                                                                                                                                     <  1:680344/151‑1 (MQ=255)
  gTTTAAAGATGGCGAAATCATATTTGTGGAGAAAACTCACAATATAAAGAATGGACAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGTTGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTac                                                                                                                                                    <  2:769669/151‑1 (MQ=255)
             gCGAAATCATATTTGTGGAGAAAACTCACAATATAAAGAATGGACAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGTTGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTaca                                                                                                                                         >  2:72691/1‑151 (MQ=255)
             gCGAAATCATATTTGTGGAGAAAACTCACAATATAAAGAATGGACAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGTTGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTaca                                                                                                                                         >  1:918264/1‑151 (MQ=255)
                 aaTCATATTTGTGGAGAAAACTCACAATATAAAGAATGGACAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCATTGTTGAAGATGATAGATTGACACTAGTTTCACTAAATAAAGATTACGACGATCTACACttt                                                                                                                                     <  1:174181/151‑1 (MQ=255)
                  aTCATATTTGTGGAGAAAACTCACAATATAAAGAATGGACAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGTTGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACtttt                                                                                                                                    <  1:601015/151‑1 (MQ=255)
                               agaAAACTCACAATATAAAGAATGGACAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGTTGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAg                                                                                                                       <  1:507378/151‑1 (MQ=255)
                                   aaCTCACAATATAAAGAATGGACAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGTTGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGtgtg                                                                                                                   <  1:1044984/151‑1 (MQ=255)
                                    aCTCACAATATAAAGAATGGACAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGTTGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGa                                                                                                                  >  1:438046/1‑151 (MQ=255)
                                     cTCACAATATAAAGAATGGACAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGTTGAAGATGATAGATTGTTTTTTTTTTAATGATACGGCGACCACCGAGATCTACACTTTTATAGAAATGAAAGTGTGAg                                                                                                                 <  2:984146/151‑1 (MQ=255)
                                      tCACAATATAAAGAATGGACAAATTGGTATATTTATCATTGAAGAAGAAGCGTACGTTAAGTAAGTCTTTGTTGAAGATGATAGATTTTTTTTTTTTTAATGATACGGCGACCACCGAGATCTACACTTTTATAGAAATGAAAgtgtagat                                                                                                                <  2:119501/151‑5 (MQ=255)
                                       cacaATATAAAGAATGGACAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGTTGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGt                                                                                                               >  1:499019/1‑151 (MQ=255)
                                       cacaATATAAAAAATGGACAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGTTGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGt                                                                                                               <  1:744527/151‑1 (MQ=255)
                                           atatAAAGAATGGACAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGTTGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAAt                                                                                                           >  1:153030/1‑151 (MQ=255)
                                            tataACGACTGGACCAATTGGTATATTCATCATTGAAGAAGAAGCTTACTTTAAGTAAGTCTTTGTTGAAGATGATAGATTGTTTTTTTTTTAATGATACGGCGACCACCGAGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAAtt                                                                                                          <  2:1052353/151‑1 (MQ=255)
                                                   aaTGGACAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGTTGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAg                                                                                                   <  1:878046/151‑1 (MQ=255)
                                                    aTGGACAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGTTGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGt                                                                                                  >  1:633556/1‑151 (MQ=255)
                                                               ggTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGTTGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATaaa                                                                                       >  2:961611/1‑151 (MQ=255)
                                                                 tataTTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGTTGAAGATGATAGATTGTTTTTTTTTTAATGATACGGCGACCACCGAGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAg                                                                                     <  2:818045/151‑1 (MQ=255)
                                                                  atatTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGTTGAAGATGATAGATTGTCTTTTTTTTAATGATACAGAGATTACCGAGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAgg                                                                                    <  2:90319/151‑1 (MQ=255)
                                                                    atTCATCATTGAAGAAGAAGCGTACGTTAAGAGAGTCTTTGTTGAAGATGATAGATTGTTTTTTTTTTAATGATACGGCGACCACCGAGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAg                                                                                  <  2:667628/151‑1 (MQ=255)
                                                                    atTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGTTGAAGATGATAGATTGTTTTTTTTTTAATGATACGGAGACCACCGAGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAg                                                                                  <  2:871160/151‑1 (MQ=255)
                                                                    atTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGGTTTTGTTTAAGATTATATGTTGACTTGATTTTGACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAg                                                                                  <  1:423605/151‑1 (MQ=255)
                                                                       catcatTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGTTGAAGATGATAGATTGTCTTTATTTTAATGAAACAGAGACTACGGAGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCAc                                                                               <  2:613493/151‑1 (MQ=255)
                                                                        atcatTTAAGAAGAAGCGTACGTTAAGTAAGTCTTTGTTGAAGATGATAGATTGTTTTTTTTTTAATGATACGGCGACCACGGAGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACt                                                                              <  2:703502/151‑1 (MQ=255)
                                                                        atcatTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGTTGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACt                                                                              <  1:573402/151‑1 (MQ=255)
                                                                        atcatTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGTTGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACt                                                                              <  1:961611/151‑1 (MQ=255)
                                                                                          tACGTTAAGAAAGTCTTTGTTGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAatatt                                                            <  1:622104/151‑2 (MQ=255)
                                                                                            cGTTAAGAAAGTCTTTGTTGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGat                                                          >  2:53808/1‑151 (MQ=255)
                                                                                                       tCTTTGTTGAAGATGATAGATTGTCTCTTGTTTCACGAAACAAAGACCACCGCGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCtt                                               <  2:304388/151‑1 (MQ=255)
                                                                                                        cTTTGTTGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTa                                              >  2:549792/1‑151 (MQ=255)
                                                                                                           tgttgAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTAGAc                                           <  1:41180/151‑1 (MQ=255)
                                                                                                            gttgAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTAGACt                                          <  1:846554/151‑1 (MQ=255)
                                                                                                              tgAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTAGACttt                                        <  1:438185/151‑1 (MQ=255)
                                                                                                              tgAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTAGACttt                                        >  1:751709/1‑151 (MQ=255)
                                                                                                              tgAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTAGACttt                                        >  1:48452/1‑151 (MQ=255)
                                                                                                                       tAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTAGACTTTGAAACTatg                               <  1:940153/151‑1 (MQ=255)
                                                                                                                        aGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTAGACTTTGAAACTatga                              <  1:53808/151‑1 (MQ=255)
                                                                                                                             gACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTAGACTTTGAAACTAtgaatgaa                         <  1:85840/151‑1 (MQ=255)
                                                                                                                                   aGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTAGACTTTGAAACTATGAATGAACATATg                   >  2:244216/1‑151 (MQ=255)
                                                                                                                                              ataaaGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTAGACTTTGAAACTATGAATGAACATATGAACAGCCCTTg        <  1:545211/151‑1 (MQ=255)
                                                                                                                                               caaaGATTACCGAGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTAGACTTTGAAACTATGAATGAACATATGAACAGCCCTTGc       <  2:1003150/150‑1 (MQ=255)
                                                                                                                                                  aGATTACGGAGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTAGACTTTGAAACTATGAATGAACATATGAACAGCCCTTGCGaa    <  2:630820/151‑1 (MQ=255)
                                                                                                                                                    aTTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTAGACTTTGAAACTATGAATGAACATATGAACAGCCCTTGCGAAGt  <  2:153030/151‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ATGTTTAAAGATGGCGAAATCATATTTGTGGAGAAAACTCACAATATAAAGAATGGACAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGTTGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTAGACTTTGAAACTATGAATGAACATATGAACAGCCCTTGCGAAGT  >  CP000730/2124374‑2124672

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑