Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,558,6360AT22.2% 104.1 / 10.1 45L14F (TTA→TTT) bcrMFS family major facilitator transporter, bicyclomycin:cation symporter
Reads supporting (aligned to +/- strand):  ref base A (31/4);  new base T (10/0);  total (41/4)
Fisher's exact test for biased strand distribution p-value = 5.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

ATTAAGAGTATAGGGATTTTTTATATAAACTTGTATTCTAAGTACATACAAATACACACAAAACGTATATAATTTATATAATTATATCATTTAAAGGAGTCTGATCATGCAATCAACGAAAACCAAAACGAAGCATTTTTCATTTTTATTGCTAATTACGTTAGGCGTCATGACCGCTTTTGGCCCACTAACTATAGATATGTACGTACCATCATTACCTAAAGTGCAAGGTGATTTTGGTTCTACTACATCAGAAATTCAATTAACATTATCATTCACAATGAT  >  CP000730/2558489‑2558773
                                                                                                                                                   |                                                                                                                                         
aTTAAGAGTATAGGGATTTTTTATATAAACTTGTATTCTAAGTACATACAAATACACACAAAACGTATATAATTTATATAATTATATCATTTAAAGGAGTCTGATCATGCAATCAACGAAAACCAAAACGAAGCATTTTTCATTTTTATTg                                                                                                                                        <  1:910921/151‑1 (MQ=255)
               aTTTTTTATATAAACTTGTATTCTAAGTACATACAAATACACACAAAACGTATATAATTTATATAATTATATCATTTAAAGGAGTCTGATCATGCAATCAACGAAAACCAAAACGAAGCATTTTTCATTTTTATTGCTAATTACGTTAGGc                                                                                                                         <  1:904006/151‑1 (MQ=255)
                                                           aaaaCGTATATAATTTATATAATTATATCATTTAAAGGAGTCTGATCATGCAATCAACGAAAACCAAAACGAAGCATTTTTCATTTTTATTGCTAATTACGTTAGGCGTCATGACCGCTTTTGGCCCACTAACTATAGATATGTACGTAcc                                                                             >  1:560735/1‑151 (MQ=255)
                                                                           atataattATATCATTTAAAGGAGTCTGATCATGCAATCAACGAAAACCAAAACGAAGCATTTTTTTTTTTTTTTGCTAATTACGATAGGCGTCATGACCGCTTTTGGCCCACTAACTATAGATATGTACGTACCATCATTACCTAAAGtt                                                             >  2:943710/1‑150 (MQ=255)
                                                                            tataattATATCATTTAAAGGAGTCTGATCATGCAATCAACGAAAACCAAAACGAAGCATTTTTTTTTTTTTTTTCTAAATACGTTAGGCGTCATGACCGCTTTTGGCCCACTAACTATAGATATGTATGTACCATCATTTCCTAAAGtac                                                            >  2:955967/1‑149 (MQ=255)
                                                                            tataattATATCATTTAAAGGAGTCTGATCATGCAATCAACGAAAACCAAAACGAAGCATTTTTCATTTTTATTGCTAATTACGTTAGGCGTCATGACCGCTTTTGGCCCACTAACTATAGATATGTATGTACCATCATTACCTAAAGTGc                                                            >  2:835554/1‑151 (MQ=255)
                                                                            tataattATATCATTTAAAGGAGTCTGATCATGCAATCAACGAAAACCAAAACGAAGCATTTTTCATTTTTATTGCTAATTACGTTAGGCGTCATGACCGCTTTTGGCCCACTAACTATAGATATGTATGTACCATCATTACCTAAAGTGc                                                            >  2:629157/1‑151 (MQ=255)
                                                                             ataattATATCATTTAAAGGAGTCTGATCATGCAATCAACGAAAACCAAAACGAAGCATTTTTCATTTTTTTTTTTAATTACGTTAGGCGTCATGACCGCTTTTGGCTCACTAACTATAGATATGTACGTACCATCATTACCTAAAGTGCa                                                           >  2:222389/1‑151 (MQ=255)
                                                                                attATATCATTTAAAGGAGTCTGATCATGCAATCAACGAAAACCAAAACGAAGCATTTTTCATTTTTATTGCTAATTACGTTAGGCGTCATGACCGCTTTTGTCCCACTAACTATAGATATGTACGTACCATCATTACCTAAAGTGCATgg                                                        >  2:793328/1‑151 (MQ=255)
                                                                                      tCATTTAAAGGAGTCTGATCATGCAATCAACGAAAACCAAAACGAAGCATTTTTCATTTTTATTGCTAATTACGTTAGGCGTCATGACCGCTTTTGGCCCACTAACTATAAATATGTACGTACCATCATTACCTAAAGTgacaagtaaaat                                                  >  2:821111/1‑140 (MQ=255)
                                                                                       cATTTAAAGGAGTCTGATCATGCAATCAACGAAAACCAAAACGAAGCATTTTTCATTTTTATTGCTAATTACGTTAGGCGTCATGACCGCTTTTGGCCCACTAACTATAGATATGTACGTACCATCATTACCTAAAGTGCAAGGTGAtttt                                                 >  2:773821/1‑151 (MQ=255)
                                                                                       cATTTAAAGGAGTCTGATCATGCAATCAACGAAAACCAAAACGAAGCATTTTTCATTTTTATTGCTAATTACGTTAGGCGTCATGACCGCTTTTGGCCCACTAACTATAGATATGTACGTACCATCATTACCTAAAGTGCAAGGTGAtttt                                                 >  1:641583/1‑151 (MQ=255)
                                                                                        aTTTAAAGGAGTCTGATCATGCAATCAACGAAAACCAAAACGAAGCATTTTTCATTTTTATTGCTAATTACGTTAGGCGTCATGACCGCTTTTGGCCCACTAACTATAGATATGTACGTACCATCATTACCTAAAGTGCAAGGTGATTTTg                                                >  1:684408/1‑151 (MQ=255)
                                                                                          ttAAAGGAGTCTGATCATGCAATCAACGAAAACCAAAACGAAGCATTTTTCATTTTTATTGCTAATTACGTTAGGCGTCATGACCGCTTTTGGCCCACTAACTATAGATATGTACGTACCATCATTACCTAAAGTGCAAGGTGATTTTGGt                                              >  1:816481/1‑151 (MQ=255)
                                                                                            aaaGGAGTCTGATCATGCAATCAACGAAAACCAAAACGAAGCATTTTTCATTTTTATTGCTAATTACGTTAGGCGTCATGACCGCTTTTGGCCCACTAACTATAGATATGTACGTACCATCATTACCTAAAGTGCAAGGTGATTTTGGTTc                                            >  1:982747/1‑151 (MQ=255)
                                                                                               ggAGTCTGATCATGCAATCAACGAAAACCAAAACGAAGCATTTTTCTTTTTTATTTCTAATTACGTTAGGCGTCATGACCGCTTTTGGCCCACTAACTATAGATATGTACGTACCATCATTACCTAAAGTGCAAGGTGATTTTGGTTctac                                         >  2:194409/1‑151 (MQ=255)
                                                                                               ggAGTCTGATCATGCAATCAACGAAAACCAAAACGAAGCATTTTTCATTTTTATTGCTAATTACGTTAGGCGTCATGACCGCTTTTGGCCCACTAACTATAGATATGTACGTACCATCATTACCTAAAGTGCAAGGTGATTTTGGTTctac                                         >  1:911724/1‑151 (MQ=255)
                                                                                                    cTGATCATGCAATCAACGAAAACCAAAACGAAGCATTTTTTTTTTTTTTTTCTAATTACGTTAGGCGTCATGACCGCTTTTGGCCCACTAACTATAGATATGTACGTACCATCATTACCTAAAGTGCAAGGTGATTTTGGTACTACTACAt                                    >  2:115808/1‑151 (MQ=255)
                                                                                                    cTGATCATGCAATCAACGAAAACCAAAACGAAGCATTTTTCATTTTTATTGCTCATTACGTTAGGCGTCCTCACCGCTTTTTGCCCACTAACATTAGATATGTACGTACCATCATTACCTAAAGTGCAAGGCGATTTTGGTGCTTCTACAt                                    >  2:892769/1‑151 (MQ=255)
                                                                                                     tGATCATGCAATCAACGAAAACCAAAACGAAGCATTTTTTTTTTTTTTTTTTAATTACGTTAGGCGTCATGACCGCTTTTTGTCCACTAACTATAGATATGTTCGTTCCATCATTACCTAAAGTGCAAAGTGATTTTGGTTCTACTACAtt                                   >  2:7438/1‑150 (MQ=255)
                                                                                                     tGATCATGCAATCAACGAAAACCAAAACGAAGCATTTTTTTTTTTTTTTTCTAATTACGTTAGGCGTCATGACCGCTTTTGGCCCACTAACTATAGATATGTACGTACCATCATTTCCTAAAGTGCAAGGTGATTTTGGTTCTACTACAtt                                   >  2:783007/1‑150 (MQ=255)
                                                                                                     tGATCATGCAATCAACGAAAACCAAAACGAAGCATTTTTCATTTTTATTGCTAATTACGTTAGGCGTCATGACCGCTTTTGGCCCACTAACTATAGATATGTACGTACCATCATTACCTAAAGTGCAAGGTGATTTTGGTTCTACTACATc                                   >  2:252905/1‑151 (MQ=255)
                                                                                                         cATGCAATCAACGAAAACCAAAACGAAGCATTTTTCTTTTTTTTTTCTAATTACGTTAGGCGTCATGACCGCTTTTGGCCCACTAACTATAGATATGTACGTACCCTCATTACCTAAAGTGCAAGGTGATTTTGGTTCTACTACATCAGaa                               >  2:246166/1‑151 (MQ=255)
                                                                                                             cAATCAACGAAAACCAAAACGAAGCATTTTTCATTTTTATTGCTAATTACGTTAGGCGTCATGACCGCTTTTGGCCCACTAACTATAGATATGTACGTACCATCATTACCTAAAGTGCAAGGTGATTTTGGTTCTACTACATCAGAAATTc                           >  1:18074/1‑151 (MQ=255)
                                                                                                             cAATCAACGAAAACCAAAACGAAGCATTTTTCATTTTTATTGCTAATTACGTTAGGCGTCATGACCGCTTTTGGCCCACTAACTATAGATATGTACGTACCATCATTACCTAAAGTGCAAGGTGATTTTGGTTCTACTACATCAGAAATTc                           >  1:797320/1‑151 (MQ=255)
                                                                                                             cAATCAACGAAAACCAAAACGAAGCATTTTTCATTTTTATTGCTAATTACGTTAGGCGTCATGACCGCTTTTGGCCCACTAACTATAGATATGTACGTACCATCATTACCTAAAGTGCAAGGTGATTTTGGTTCTACTACATCAGAAATTc                           >  2:677390/1‑151 (MQ=255)
                                                                                                             cAATCAACGAAAACCAAAACGAAGCATTTTTCATTTTTATTGCTAATTACGTTAGGCGTCATGACCGCTTTTGGCCCACTAACTATAGATATGTACGTACCATCATTACCTAAAGTGCAAGGTGATTTTGGTTCTACTACATCAGAAATTc                           >  1:691919/1‑151 (MQ=255)
                                                                                                              aaTCAACGAAAACCAAAACGAAGCATTTTTCATTTTTATTGCTAATTACGTTAGGCGTCATGACCGCTTTTGGCCCACTAACTATAGATATGTACGTACCATCATTACCTAAAGTGCAAGGTGATTTTGGTTCTACTACATCAGAAATTCa                          >  1:174615/1‑151 (MQ=255)
                                                                                                              aaTCAACGAAAACCAAAACGAAGCATTTTTCATTTTTATTGCTAATTACGTTAGGCGTCATGACCGCTTTTGGCCCACTAACTATAGATATGTACGTACCATCATTACCTAAAGTGCAAGGTGATTTTGGTTCTACTACATCAGAAATTCa                          >  1:877258/1‑151 (MQ=255)
                                                                                                              aaTCAACGAAAACCAAAACGAAGCATTTTTCATTTTTATTGCTAATTACGTTAGGCGCCATGACCGCTTTTGGCCCACTAACTATAGATATGTACGTACCATCCTTACCTAAAGTGCAAGGTGCTTCTGGTTCTACTACATCATAAATTCa                          >  2:184645/1‑151 (MQ=255)
                                                                                                               aTCAACGAAAACCAAAACGAAGCATTTTTCATTTTTATTGCTAATTACGTTAGGCGTCATGACCGCTTTTGGCCCACTAACTATAGATATGTACGTACCATCATTACCTAAAGTGCAAGGTGATTTTGGTTCTACTCCATCAGAAATTCaa                         >  2:749779/1‑151 (MQ=255)
                                                                                                                tCAACGAAAACCAAAACGAAGCATTTTTCATTTTTATTGCTAATTACGTTAGGCGTCATGACCGCTTTTGGCCCACTAACTATAGATATGTACGTACCATCATTACCTAAAGTGCAAGGTGATTTTGGTTCTACTACATCAGAAATTCAAt                        >  1:444006/1‑151 (MQ=255)
                                                                                                                  aaCGAAAACCAAAACGAAGCATTTTTCATTTTTATTGCTAATTACGTTAGGCGTCATGACCGCTTTTGGCCCACTAACTATAGATATGTACGTACCATCATTACCTAAAGTGCAAGGTGATTTTGGTTCTACTACATCAGAAATTCAATTa                      >  1:261560/1‑151 (MQ=255)
                                                                                                                    cGAAAACCAAAACGAAGCATTTTTCATTTTTATTGCTAATTACGTTAGGCGTCATGACCGCTTTTGGCCCACTAACTATAGATATGTACGTACCATCATTACCTAAAGTGCAAGGTGATTTTGGTTCTACTACATCAGAAATTCAATTAAc                    >  2:1020532/1‑151 (MQ=255)
                                                                                                                      aaaaCCAAAACGAAGCATTTTTTTTTTTTTTTTCTAATTACGATAGGCGTCATGACCGCTTTTGGCCCACTAACTATAGATATGTACGTACCCTCATTACCTAAAGTACAAGGTGATTTTGTTTCTTCTACTTCAGAAATTCAATTAACAt                  >  2:865572/1‑151 (MQ=255)
                                                                                                                         aCCAAAACGAAGCATTTTTCATTTTTATTGCTAATTACGTTAGGCGTCATGACCGCTTTTGGCCCACTAACTATAGATATGTACGTACCATCATTACCTAAAGTGCAAGGTGATTTTGGTTCTACTACATCAGAAATTCAATTAACATTAt               <  1:111308/151‑1 (MQ=255)
                                                                                                                          ccAAAACGAAGCATTTTTTATTTTTTTTGCTAATTACGTTAGGCGTCATGACCGCTTTTGGCCCACTAACTATAGATATGTACGTACCATCATTACCTAAAGTGCAAGGTGATTTTGGTTCTACTACATCAGAAATTCAATTAACATTATc              >  2:943090/1‑151 (MQ=255)
                                                                                                                            aaaaCGAAGCATTTTTCATTTTTATTGCTAATTACGTTAGGCGTCATGACCGCTTTTGGCCCACTAACTATAGATATGTACGTACCATCATTACCTAAAGTGCAAGGTGATTTTGGTTCTACTACATCAGAAATTCAATTAACATTATCAt            >  1:144104/1‑151 (MQ=255)
                                                                                                                                cGAAGCATTTTTCATTTTTATTGCTAATTACGTTAGGCGTCATGACCGCTTTTGGCCCACTAACTATAGATATGTACGTAACATCATTACCTAAAGTGCAAGGTGATTTTGGTTATAATACATAAGAACTTCAATTACAATTATTATTcac        >  1:316781/1‑151 (MQ=255)
                                                                                                                                 gAAGCATTTTTCATTTTTATTGCTAATTACGTTAGGCGTCATGACCGCTTTTGGCCCACTAACTATAGATATGTACGTACCATCATTACCTAAAGTGCAAGGTGATTTTGGTTCTACCACATCAGAAATTCAATTAACATTATCATTcaca       >  1:89769/1‑151 (MQ=255)
                                                                                                                                  aaGCATTTTTCATTTTTATTGCTAATTACGTTAGGCGTCATGACCGCTTTTGGCCCACTAACTATAGATATGTACGTACCATCATTACCTAAAGTGCAAGGTGATTTTGGTTCTACTACATCAGAAATTCAATTAACATTATCATTCACaa      >  1:1034161/1‑151 (MQ=255)
                                                                                                                                   aGCATTTTTCATTTTTATTGCTAATTACGTTAGGCGTCATGACCGCTTTTGGCCCACTAACTATAGATATGTACGTACCATCATTACCTAAAGTGCAAGGTGATTTTGGTTCTACTACATCAGAAATTCAATTAACATTATCATTCACAAt     >  2:233617/1‑151 (MQ=255)
                                                                                                                                    gCATTTTTTTTTTTTTTTTCTAATTACGTTAGGCGTCATGACCGCTTTTGGCCCACTAACTATAGATATGTACGCACCATCATTACCTAAATTGTTATGTTATTTTGGTTCTACTACATCAGAAATTCAATTAACATTATCATTCACAAtt    >  2:705161/1‑150 (MQ=255)
                                                                                                                                     cattttttttttttATTTCAAATTACGATAGGCGTCATGACCGCTTTTGGCCCCCTAACTATAGATATGTACGTACCATCATTACCTAATGTGCAATGTGATTTTTGTTCCACTACATCAGAAATTCATTTAACATTATCATTCACAATGa   >  2:644763/1‑151 (MQ=255)
                                                                                                                                      atttttcatttttATTGCTAATTACGTTAGGCGTCATGACCGCTTTTGGCCCACTAACTATAGATATGTACGTACCATCATTACCTAAAGTGCAAGGTGATTTTGGTTCTACTACATCAGAAATTCAATTAACATTATCATTCACAATGAt  <  2:641583/151‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                         
ATTAAGAGTATAGGGATTTTTTATATAAACTTGTATTCTAAGTACATACAAATACACACAAAACGTATATAATTTATATAATTATATCATTTAAAGGAGTCTGATCATGCAATCAACGAAAACCAAAACGAAGCATTTTTCATTTTTATTGCTAATTACGTTAGGCGTCATGACCGCTTTTGGCCCACTAACTATAGATATGTACGTACCATCATTACCTAAAGTGCAAGGTGATTTTGGTTCTACTACATCAGAAATTCAATTAACATTATCATTCACAATGAT  >  CP000730/2558489‑2558773

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑