Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	1,232,053	A→T	D233V (GAT→GTT)	prpC →	possible PP2C protein phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,232,053	0	A	T	100.0%	145.8 / NA	41	D233V (GAT→GTT)	prpC	possible PP2C protein phosphatase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (21/20);  total (21/20)

GATTATTTATTATTAAATTCAGATGGATTAACTGATTATGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAAGATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTA  >  CP000730/1231914‑1232198                                                                                                                                            |                                                                                                                                                  gATTATTTATTATTAAATTCAGATGGATTAACTGATTATGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAAGTTAACGTTACtt                                                                                                                                        <  1:736496/151‑1 (MQ=255)    tatttattATTAAATTCAGATGGATTAACTGATTATGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAAGTTAACGTTACTTTCa                                                                                                                                     <  1:318088/151‑1 (MQ=255)            attaAATTCAGATGGATTAACTGATTATGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAAGTTAACGTTACTTTCATACTcgcg                                                                                                                             >  1:134015/1‑151 (MQ=255)            attaAATTCAGATGGATTAACTGATTATGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAAGTTAACGTTACTTTCATACTcgcg                                                                                                                             >  1:449881/1‑151 (MQ=255)                      gATGGATTAACTGATTATGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAAGTTAACGTTACTTTCATACTCGCGGCTATTGAAg                                                                                                                   >  1:548535/1‑151 (MQ=255)                       aTGGATTAACTGATTATGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAAGTTAACGTTACTTTCATCCTCGCGGCTATTGAAgg                                                                                                                  <  2:483594/151‑1 (MQ=255)                       aTGGATTAACTGATTATGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAAGTTAACGTTACTTTCATACTCGCGGCTATTGAAgg                                                                                                                  <  2:529795/151‑1 (MQ=255)                                cTGATTATGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAAGTTAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGt                                                                                                         >  1:29706/1‑151 (MQ=255)                                 tGATTATGTTAAAGACAATGAAATTAAGCGATTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAAGTTAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTa                                                                                                        >  2:973785/1‑151 (MQ=255)                                    ttATGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAAGTTAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGa                                                                                                     <  2:768112/151‑1 (MQ=255)                                       tGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAAGTTAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAg                                                                                                  >  2:376834/1‑151 (MQ=255)                                        gtcaaaGACAATGAAATTAAGCATTTGTTAGTAAAAGAAGGTACCATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAAGTTAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAgg                                                                                                 <  1:890793/148‑1 (MQ=255)                                         ttAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAAGTTAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGt                                                                                                >  2:299134/1‑151 (MQ=255)                                                  aTGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAAGTTAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAataata                                                                                       >  2:541950/1‑151 (MQ=255)                                                      aaTTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAAGTTAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATg                                                                                   <  1:745445/151‑1 (MQ=255)                                                              gTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAAGTTAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGatat                                                                           <  1:716402/151‑1 (MQ=255)                                                                  gTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAAGTTAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATaaaa                                                                       >  2:837247/1‑151 (MQ=255)                                                                   ttAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAAGTTAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAAt                                                                      <  2:425128/151‑1 (MQ=255)                                                                     aGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAAGTTAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTg                                                                    <  2:211295/151‑1 (MQ=255)                                                                      gTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAAGTTAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGt                                                                   >  2:460795/1‑151 (MQ=255)                                                                        aaaaGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAAGTTAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAg                                                                 >  2:883337/1‑151 (MQ=255)                                                                        aaaaGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAAGTTAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAg                                                                 <  1:802098/151‑1 (MQ=255)                                                                              agGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAAGTTAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGc                                                           >  1:1595/1‑151 (MQ=255)                                                                               ggTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAAGTTAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCt                                                          >  1:430874/1‑151 (MQ=255)                                                                                   cAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAAGTTAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTggc                                                      <  1:54004/151‑1 (MQ=255)                                                                                   cAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAAGTTAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTggc                                                      <  1:48662/151‑1 (MQ=255)                                                                                        gaagaTCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAAGTTAACGTTACTTTCATACTCGCGGCTCTTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGCTAAGCTTGGcggcgg                                                 >  2:481867/1‑151 (MQ=255)                                                                                        gaagaTCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAAGTTAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGcggcgg                                                 >  2:961233/1‑151 (MQ=255)                                                                                                       cAATTAATGCAATTGGCATTAGATAACCATTCGAAAGTTAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATGAGTACCGt                                  <  1:620910/151‑1 (MQ=255)                                                                                                        aaTTAATGCAATTGGCATTAGATAACCATTCGAAAGTTAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATGAGTACCGtt                                 <  2:449881/151‑1 (MQ=255)                                                                                                             aTGCAATTGGCATTAGATAACCATTCGAAAGTTAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATGAGTACCGTTTATCt                            >  1:900108/1‑151 (MQ=255)                                                                                                                  aTTGGCATTAGATAACCATTCGAAAGTTAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATGAGTACCGTTTATCTTGCTg                       <  1:376834/151‑1 (MQ=255)                                                                                                                      gCATTAGATAACCATTCGAAAGTTAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATGAGTACCGTTTATCTTGCTGAAga                   <  1:541950/151‑1 (MQ=255)                                                                                                                        aTTAGATAACCATTCGAAAGTTAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATGAGTACCGTTTATCTTGCTGAAgata                 <  2:65867/151‑1 (MQ=255)                                                                                                                        aTTAGATAACCATTCGAAAGTTAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATGAGTACCGTTTATCTTGCTGAAgata                 >  1:704059/1‑151 (MQ=255)                                                                                                                                  cATTCGAAAGTTAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTaa       >  2:477151/1‑151 (MQ=255)                                                                                                                                     tCGAAAGTTAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACAt    <  2:156574/151‑1 (MQ=255)                                                                                                                                      cGAAAGTTAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACAtt   >  2:329234/1‑151 (MQ=255)                                                                                                                                       gAAAGTTAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATTATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTa  >  2:914053/1‑151 (MQ=255)                                                                                                                                       gAAAGTTAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAaataa                                                                                         >  2:787352/1‑64 (MQ=255)                                                                                                                                       gAAAGTTAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAaataa                                                                                         <  1:787352/64‑1 (MQ=255)                                                                                                                                            |                                                                                                                                                  GATTATTTATTATTAAATTCAGATGGATTAACTGATTATGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAAGATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTA  >  CP000730/1231914‑1232198

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑