Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	584,121	G→T	intergenic (+205/‑67)	rplA → / → rplJ	ribosomal protein L1/ribosomal protein L10

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	584,121	0	G	T	100.0%	80.6 / NA	26	intergenic (+205/‑67)	rplA/rplJ	ribosomal protein L1/ribosomal protein L10
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (19/7);  total (19/7)

CAATAAGTAATTTCCAAGTTATATTACTTATTGTGATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCGTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCAAATTCAGTTT  >  CP000730/583978‑584257                                                                                                                                                |                                                                                                                                         cAATAAGTAATTTCCAAGTTATATTACTTATTGTGATTATTTTACGTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGTCTATAATTGACTTTAACGTGATGATCTATGATCTTTCAAGCACTTTTTTCCTTGGGTAg                                                                                                                                   >  2:261070/1‑151 (MQ=255)   aTAAGTAATTTCCAAGTTATATTACTTATTGTGATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGaa                                                                                                                                 >  2:207592/1‑151 (MQ=255)      aGTAATTTCCAAGTTATATTACTTATTGTGATTATTTTACCTAAGACAGAAGGATTTATTTATAACTTAAAATTTATCCTGTCGAGGCTAAAATTGACATAAACGTGATAATCTATGATCTTTCAAGCACTTTTTACCTTGGGTAGAAAGt                                                                                                                              >  2:57419/1‑151 (MQ=255)           tttCCAAGTTATATTACTTATTGTGATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCttt                                                                                                                         >  1:77805/1‑151 (MQ=255)                              aTTGTGATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTaaaaaa                                                                                                      >  2:87185/1‑151 (MQ=255)                              aTTGTGATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTaaaaaa                                                                                                      >  2:87134/1‑151 (MQ=255)                                           tACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAAtt                                                                                         >  1:77292/1‑151 (MQ=255)                                              cTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTaa                                                                                      >  2:49124/1‑151 (MQ=255)                                               tAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTaaa                                                                                     >  1:288048/1‑151 (MQ=255)                                                 aGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGAAAAAAAAATTTAAATg                                                                                   <  2:127016/151‑1 (MQ=255)                                                                        ttAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATc                                                            <  2:77805/151‑1 (MQ=255)                                                                        ttAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATc                                                            >  2:454208/1‑151 (MQ=255)                                                                               ttATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAg                                                     >  1:210194/1‑151 (MQ=255)                                                                                        cGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAaac                                            >  1:78098/1‑151 (MQ=255)                                                                                         gAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAaaca                                           <  1:87134/151‑1 (MQ=255)                                                                                         gAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAaaca                                           <  1:87185/151‑1 (MQ=255)                                                                                                     gACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAATAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTtgatga                               <  1:48710/151‑1 (MQ=255)                                                                                                            aCGTGATGATCTATGATCTTTCAAGCACTTTTTTTTTTTGTTTGAAAGAGCGTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGTAGTCTGAATGTCTGCTATAATTGAAGTTAAAAAACAACTAGTTGTTGTAATTGCt                        >  2:139192/1‑151 (MQ=255)                                                                                                            aCGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTTTTTTTTTTTAAAAAAGCACCAAAAATTTAAATGTAGTTTTCTGGATGTCTGCTCTCATTGTTGCTCTAACACAATTAGTTGATGAAATTGCt                        >  2:235636/1‑151 (MQ=255)                                                                                                               tgatgaTCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTTATTTTAAAAAAAGCACAAAAAATATAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAg                     >  2:395522/1‑151 (MQ=255)                                                                                                               tgatgaTCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAg                     <  1:257171/151‑1 (MQ=255)                                                                                                                   gatctatgatctTTCAAGCACTTTTTTTTTTTTTTTGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTCCTGAGGTAc                 >  2:173598/1‑151 (MQ=255)                                                                                                                         tgatctTTCAAGCACTTTTTGCTTTGGGTAGAAAGTGCTTTTTTTTTTTATTTTTAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTTAATGTCTGCTATCATTTAAGCTAAAAATCAACTATTTGTTGAAATTGCTTAGGTACTATCaa           >  2:176236/1‑151 (MQ=255)                                                                                                                           atctTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCAAAt         <  1:280849/151‑1 (MQ=255)                                                                                                                                tCAAGCACTTGTGGCCTTTGGTAGAAAGTGCTTTTTTTGTTGATTTTAAAAAAAGCACCAAAAATTTAAATGGATGTGTCTGAATGTCTGCTATCATTGATGCTAAAAAACAACTAGTTGATGAAATTGCTGAGATACTATCAAATTCaat    >  2:86112/1‑149 (MQ=255)                                                                                                                                  aaGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTCTAAAAAAAGCACCAAAAATTTCACTGTAGGTGTCTGAATCTCTGCTATCATTCAAGCTAAAAACCAACTACTTGATGAAATTGCTCAGGTACTATCAACTTCAGttt  >  2:339499/1‑151 (MQ=255)                                                                                                                                                |                                                                                                                                         CAATAAGTAATTTCCAAGTTATATTACTTATTGTGATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCGTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCAAATTCAGTTT  >  CP000730/583978‑584257

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑