Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 116,951 T→C 7.8% G356G (GGT→GGC)  amd → M20 family peptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730116,9510TC 7.8% 142.4 / 2.9 53G356G (GGT→GGC) amdM20 family peptidase
Reads supporting (aligned to +/- strand):  ref base T (25/23);  new base C (2/2);  total (28/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01

GGCTAAGACGTTGAAAGAAGCAAACCTTGATTTTGGTGTTGAAATGTGTGAACCACAACCACCTTCAGAAGACTTTGCATATTATGCTAAAGAACGCCCAAGTGCCTTTATTTATACAGGTGCAGCGGTGGAAAATGGTGAAATTTACCCACATCATCATCCTAAATTTAACATTTCAGAAAAATCATTACTTATTTCTGCAGAAGCAGTAGGGACAGTTGTTTTAGATTACCTTAAAGGAGATAACTAACATGAATGAAACGTATCGCGGGGGC  >  CP000730/116813‑117087
                                                                                                                                          |                                                                                                                                        
ggCTAAGACGTTTAAAGAAGCGAACCTTGATTTTGGTGCTGAAATGTGTGCACCACAACCACCTTCAGAAGCCTTTGCATATTATGGTAAAGAAGGCCCAAGCACCTTTATTTATACAGGTGCAGCGGTGGGAAAGGGTGa                                                                                                                                        >  2:486260/1‑141 (MQ=255)
 gCTAAGACGTTGAAAGAAGCAAACCTTGATTTTGGTGTTGAAATGTGTGAACCACAACCACCTTCAGAAGACTTTGCATATTATGCTAAAGAACGCCCAAGTGCCTTTATTTATACAGGTGCAGCGGTGGAAAATGGTGaa                                                                                                                                       >  1:37922/1‑141 (MQ=255)
   tAAGACGTTGAAAGAAGCAAACCTTGATTTTGGTGTTGAAATGTGTGAACCACAACCACCTTCAGAAGACTTTGCATATTATGCTAAAGAACGCCCAAGTGCCTTTATTTATACAGGTGCAGCGGTGGAAAATGGTGAAAt                                                                                                                                     <  1:455410/141‑1 (MQ=255)
   tAAGACGTTGAAAGAAGCAAACCTTGATTTTGGTGTTGAAATGTGTGAACCACAACCACCTTCAGAAGACTTTGCATATTATGCTAAAGAACGCCCAAGTGCCTTTATTTATACAGGTGCAGCGGTGGAAAATGGTGAAAt                                                                                                                                     <  2:265448/141‑1 (MQ=255)
    aaGACGTTGAAAGAAGCAAACCTTGATTTTGGTGTTGAAATGTGTGAACCACAACCACCTTCAGAAGACTTTGCATATTATGCTAAAGAACGCCCAAGTGCCTTTATTTATACAGGTGCAGCGGTGGAAAATGGTGAAAtt                                                                                                                                    <  1:450060/141‑1 (MQ=255)
       ccGTTGAAAGAAGCCAACCTTGACATGGTTGTTGAAATGTGTGAACCACAACCAACTTCAGAAGACTTTGCATATTATGCTAAAGAACGCCCAAGTGCCTTTATTTATACAGGTGCAGCGGTGGAAAATGGTGAAATTTAc                                                                                                                                 <  1:528682/140‑1 (MQ=255)
                 aagCAAACCTTGAGTTTGGTGTTGAAATGTGTGAACCACAACCACCTTCAGAAGACTTTGCATATTATGCTAAAGAACGCCCAAGTGCCTTTATTTATACAGGTGCAGCGGTGGAAAATGGTGAAATTTACCCAcatcatc                                                                                                                       <  2:202919/141‑1 (MQ=255)
                  agCAAACCTTGATTTTGGTGTTGAAATGTGTGAACCACAACCACCTTCAGAAGACTTTGCATATTATGCTAAAGAACGCCCAAGTGCCTTTATTTATACAGGTGCAGCGGTGGAAAATGGTGAAATTTACCCAcatcatca                                                                                                                      <  2:532666/141‑1 (MQ=255)
                  agCAAACCTTGATTTTGGTGTTGAAATGTGTGAACCACAACCACCTTCAGAAGACTTTGCATATTATGCTAAAGAACGCCCAAGTGCCTTTATTTATACAGGTGCAGCGGTGGAAAATGGTGAAATTTACCCAcatcatca                                                                                                                      >  1:169108/1‑141 (MQ=255)
                  agCAAACCTTGATTTTGGTGTTGAAATGTGTGAACCACAACCACCTTCAGAAGACTTTGCATATTATGCTAAAGAACGCCCAAGTGCCTTTATTTATACAGGTGCAGCGGTGGAAAATGGCGAAATTTACCCAcatcatca                                                                                                                      >  1:406015/1‑141 (MQ=255)
                  agCAAACCTTGATTTTGGTGTTGAAATGTGTGAACCACAACCACCTTCAGAAGACTTTGCATATTATGCTAAAGAACGCCCAAGTGCCTTTATTTATACAGGTGCAGCGGTGGAAAATGGCGAAATTTACCCAcatcatca                                                                                                                      >  1:405987/1‑141 (MQ=255)
                            gATTTTGGTGTTGAAATGTGTGAACCACAACCACCTTCAGAAGACTTTGCATATTATGCTAAAGAACGCCCAAGTGCCTTTATTTATACAGGTTCAGCGGTGGAAAATGGTGAAATTTACCCACATCATCATCCTAAAttt                                                                                                            <  1:135561/141‑1 (MQ=255)
                            gATTTTGGTGTTGAAATGTGTGAACCACAACCACCTTCAGAAGACTTTGCATATTATGCTAAAGAACGCCCAAGTGCCTTTATTTATACAGGTGCAGCGGTGGAAAATGGTGAAATTTACCCACATCATCATCCTAAAttt                                                                                                            <  1:488461/141‑1 (MQ=255)
                                  ggTGTTGAAATGTGTGAACCACAACCACCTTCAGAAGACTTTGCATATTATGCTAAAGAACGCCCAAGTGCCTTTATTTATACAGGTGCAGCGGTGGAAAATGGTGAAATTTACCCACATCATCATCCTAAATTTAACAtt                                                                                                      <  1:398421/141‑1 (MQ=255)
                                         aaaTGTGTGAACCACAACCACCTTCAGAAGACTTTGCATATTATGCTAAAGAACGCCCAAGTGCCTTTATTTATACAGGTGCAGCGGTGGAAAATGGTGAAATTTACCCACATCATCATCCTAAATTTAACATTTCAGaaa                                                                                               <  1:475728/141‑1 (MQ=255)
                                             gtgtgAACCACAACCACCTTCAGAAGACTTTGCATATTATGCTAAAGAACGCCCAAGTGCCTTTATTTATACAGGTGCAGCGGTGGAAAATGGTGAAATTTACCCACATCATCATCCTAAATTTAACATTTCAGaaaaagc                                                                                           >  2:82644/1‑139 (MQ=255)
                                             gtgtgAACCACAACCACCTTCAGAAGACTTTGCATATTATGCTAAAGAACGCCCAAGTGCCTTTATTTATACAGGTGCAGCGGTGGAAAATGGTGAAATTTACCCACATCATCATCCTAAATTTAACATTTCAGAAAAATc                                                                                           >  2:74038/1‑141 (MQ=255)
                                               gtgAACCACAACCACCTTCAGAAGACTTTGCATATTATGCTAAAGAACGCCCAAGTGCCTTTATTTATACAGGTGCAGCGGTGGAAAATGGTGAAATTTACCCACATCATTATCCTAAATTTAACATTTCAGAAAAATCAt                                                                                         <  1:476839/141‑1 (MQ=255)
                                               gtgAACCACAACCACCTTCAGAAGACTTTGCATATTATGCTAAAGAACGCCCAAGTGCCTTTATTTATACAGGTGCAGCGGTGGAAAATGGTGAAATTTACCCACATCATCATCCTAAATTTAACATTTCATAAAAATCAt                                                                                         >  1:331698/1‑141 (MQ=255)
                                               gtgAACCACAACCACCTTCAGAAGACTTTGCATATTATGCTAAAGAACGCCCAAGTGCCTTTATTTATACAGGTGCAGCGGTGGAAAATGGTGAAATTTACCCACATCATCATCCTAAATTTAACATTTCAGAAAAATCAt                                                                                         >  1:543518/1‑141 (MQ=255)
                                               gtgAACCACAACCACCTTCAGAAGACTTTGCATATTATGCTAAAGAACGCCCAAGTGCCTTTATTTATACAGGTGCAGCGGTGGAAAATGGTGAAATTTACCCACATCATCATCCTAAATTTAACATTTCAGAAAAATCAt                                                                                         >  1:170239/1‑141 (MQ=255)
                                                 gAACCACAACCACCTTCAGAAGACTTTGCATATTATGCTAAAGAACGCCCAAGTGCCTTTATTTATACAGGTGCAGCGGTGGAAAATGGTGAAATTTACCCACATCATCATCCTAAATTTAACATTTCAGAAAAATCATTa                                                                                       <  1:437520/141‑1 (MQ=255)
                                                  aaccacaaccacCTTCAGAAGACTTTGCATATTATGCTAAAGAACGCCCAAGTGCCTTTATTTATACAGGTGCAGCGGTGGAAAATGGTGAAATTTACCCACATCATCATCCTAAATTTAACATTTCAGAAAAATAATTAc                                                                                      >  1:34894/1‑141 (MQ=255)
                                                       caaccacCTTCAGAAGACTTTGCATATTATGCTAAAGAACGCCCAAGTGACTTTATTTATACAGGTGCAGCGGTGGAAAATGGTGAAATTTACCCACATCATCATCCTAAATTTAACATTTCAGAAAAATCATTACTTAtt                                                                                 >  1:330750/1‑141 (MQ=255)
                                                             ccTTCAGAAGACTTTGCATATTATGCTAAAGAACGCCCAAGTGCCTTTATTTATACAGGTGCAGCGGTGGAAAATGGTGAAATTTACCCACATCATCATCCTAAATTTAACATTTCAGAAAAATCATTACTTATTTCTGCa                                                                           >  1:32809/1‑141 (MQ=255)
                                                                 cAGAAGACTTTGCATATTATGCTAAAGAACGCCCAAGTGCCTTTATTTATACAGGTGCAGCGGTGGAAAATGGTGAAATTTACCCACCTCATCATCCTAAATTTAACATTTCAGCAAAATCATTACTAATTTCTCCAGAAg                                                                       >  2:359932/1‑141 (MQ=255)
                                                                    aagaCTTTGCATATTATGCTAAAGAACGCCCAAGTGCCTTTATTTATACAGGTGCAGCGGTGGAAAATGGTGAAATTTACCCACATCATCATCCTAAATTTAACATTTCAGAAAAATCATTACTTACTTCTGCAGAAGCAg                                                                    <  1:312043/141‑1 (MQ=255)
                                                                        cTTTGCATATTATGCTAAAGAACGCCCAAGTGCCTTTATTTATACAGGTGCAGCGGTGGAAAATGGTGAAATTTACCCACATCATCATCCTAAATTTAACATTTCAGAAAAATCATTACTTATTTCTGCAGAAGCAGTAgg                                                                >  1:290655/1‑141 (MQ=255)
                                                                          tgggatggtaggCTAGAGAACGCCCAAGTGCCTTGATTTATACAGGTGCAGCGGTGGAAAATGGTGAAATTTACCCACATCATCATCCTAAATTTAAAAAAAAAAAAAAATCATTACTTATTTCTGCAGAAGCAGTAGGGa                                                              <  2:169108/130‑1 (MQ=255)
                                                                            gCATATTATGCTAAAGAACGCCCAAGTGCCTTTATTTATACAGGTGCAGCGGTGGAAAATGGTGAAATTTACCCACATCATCATCCTAAATTTAACATTTCAGAAAAATCATTACTTATTTCTGCAGAAGCAGTAGGGACa                                                            <  1:405150/141‑1 (MQ=255)
                                                                             cATATTATGCTAAAGAACGCCCAAGTGCCTTTATTTATACAGGTGCAGCGGTGGAAAATGGTGAAATTTACCCACATCATCATCCTAAATTTAACATTTCAGAAAAATCATTACTTATTTCTGCAGAAGCAGTAGGGACAg                                                           <  2:119323/141‑1 (MQ=255)
                                                                                   atGCTAAAGAACGCCCAAGTGCCTTTATTTATACAGGTGCAGCGGTGGAAAATGGTGAAATTTACCCACATCATCATCCTAAATTTAACATTTCAGAAAAATCATTACTTATTTCTGCAGAAGCAGTAGGGACAGTTGttt                                                     >  1:178734/1‑141 (MQ=255)
                                                                                   atGCTAAAGAACGCCCAAGTGCCTTTATTTATACAGGTGCAGCGGTGGAAAATGGTGAAATTTACCCACATCATCATCCTAAATTTAACATTTCAGAAAAATCATTACTTATTTCTGCAGAAGCAGTAGGGACAGTTGttt                                                     <  1:273965/141‑1 (MQ=255)
                                                                                   atGCTAAAGAACGCCCAAGTGCCTTTATTTATACAGGTGCAGCGGTGGAAAATGGTGAAATTTACCCACATCATCATCCTAAATTTAACATTTCAGAAAAATCATTACTTATTTCTGCAGAAGCAGTAGGGACAGTTGttt                                                     <  2:101230/141‑1 (MQ=255)
                                                                                   atGCTAAAGAACACCCTAGTGCCTTTATTTATACGGGTGCAGCAGTGGACAATGGTGACATATACCCACATCAACATCCTAAACGTAACATTTCAGAAATATCATTACTTATTGCTGCAGAAGCAGTAGGGACAGTTGttt                                                     <  2:418228/141‑1 (MQ=255)
                                                                                        aaaGAACGCCCAAGTGCCTTTATTTATACAGGTGCAGCGGTGGAAAATGGTGAAATTTACCCACATCATCATCCTAAATTTAACATTTCAGAAAAATCATTACTTATTTCTGCAGAAGCAGTAGGGACAGTTGTTTTAGAt                                                <  1:124964/141‑1 (MQ=255)
                                                                                        aaaGAACGCCCAAGTGCCTTTATTTATACAGGTGCAGCGGTGGAAAATGGCGAAATTTACCCACATCATCATCCTAAATTTAACATTTCTGAAAAATCATTACTTATTTCTGCAGAAGCAGTAGGGACAGTTGTTTTAGAt                                                <  2:406015/141‑1 (MQ=255)
                                                                                        aaaGAACGCCCAAGTGCCTTTATTTATACAGGTGCAGCGGTGGAAAATGGCGAAATTTACCCACATCATCATCCTAAATTTAACATTTCAGAAAAATCATTACTTATTTCTGCAAATGCATTAGTTACAGTTGTTTTAGAt                                                <  2:405987/141‑1 (MQ=255)
                                                                                             aCGCCCAAGTGCCTTTATTTATACAGGTGCAGCGGTGGAAAATGGTGAAATTTACCCACATCATCATCCTAAATTTAACATTTCAGAAAAATCATTACTTATTTCTGCAGAAGCAGTAGGGACAGTTGTTTTAGATTACCt                                           >  1:462912/1‑141 (MQ=255)
                                                                                               ccccAAGTGTCTTTATTTATACAGGTGCAGCGGTGGAAAATGGTGAAATTTACCCACATCATCATCCTAAATTTAACATTTCAGAAAAATCATTACTTATTTCTGCAGAAGCAGTAGGGACAGTTGTTTTAGATTACCTTa                                         <  1:490971/140‑1 (MQ=255)
                                                                                                cccAAGTGCCTTTATTTATACAGGTGCAGCGGTGGAAAATGGTGAAATTTACCCACATCATCATCCTAAATTTAACATTTCAGAAAAATCATTACTTATTTCTGCAGAAGCAGTAGGGACAGTTGTTTTAGATTACCTTaa                                        >  1:473569/1‑141 (MQ=255)
                                                                                                cccAAGTGCCTTTATTTATACAGGTGCAGCGGTGGAAAATGGTGAAATTTACCCACATCATCATCCTAAATTTAACATTTCAGAAAAATCAGTACTTATTTCTGCAGAAGCAGTAAGGACAGTTGTTTTAGATTCCCTTaa                                        >  1:218408/1‑141 (MQ=255)
                                                                                                     gTGCCTTTATTTATACAGGTGCAGCGGTGGAAAATGGAGAAGATTCACGACATCAGGATCCGAAATTTGACATTTCAGAGAAATCATTACTTATTTATGCAGAAGCTGTAGGGACAGTTGTTTTGGATTACTTTATAGgaa                                   >  2:374281/1‑140 (MQ=255)
                                                                                                       gacTTTATTTATACAGGTGCAGCGGTGGAAAATGGTGAAATTTACCCACATCATCATCCTAAATTTAACATTTCAGAAAAATCATTACTTATTTCTGCAGAAGCAGTAGGGACAGTTGTTTTAGATTACCTTAAAGGAGAt                                 <  2:245800/139‑1 (MQ=255)
                                                                                                          tttatttaTACAGGTGCAGCGGTGGAAAATGGTGAAATTTACCCACATCATCATCCTAAATTTAACATTTCAGAAAAATCATTACTTATTTCTGCAGAAGCAGTAGGGACAGTTGTTTTAGATTACCTTAAAGGAGAtaaa                              >  2:182571/1‑140 (MQ=255)
                                                                                                                         gCAGCGGTGGAAAATGGTGAAATTTACCCACATCATCATCCTAAATTTAACATTTCAGAAAAATCATTACTTATTTCTGCAGAAGCAGTAGGGACAGTTGTTTTAGATTACCTTAAAGGAGATAACTAACATGAATGaaaa               >  2:397756/1‑140 (MQ=255)
                                                                                                                          cAGCGGTGGAAAATGGTGAAATTTACCCACATCATCATCCTAAATTTAACATTTCAGAAAAATCATTACTTATTTCTGCAGAAGCAGTAGGGACAGTTGTTTTAGATTACCTTAAAGGAGATAACTAACATGAATGAAACg              >  1:340657/1‑141 (MQ=255)
                                                                                                                            gCGGTGGAAAATGGTGAAATTTACCCACATCATCATCCTAAATTTAACATTTCAGAAAAATCATTACTTATTTCTGCAGAAGCAGTAGGGACAGTTGTTTTAGATTACCTTAAAGGAGATAACTAACATGAATGAAACGTa            >  2:415693/1‑141 (MQ=255)
                                                                                                                              ggTGGAAAATGGTGAAATTTACCCACATCATCATCCTAAATTTAACATTTCAGAAAAATCATTACTTATTTCTGCAGAAGCAGTAGGGAGAGTTGTTTTATATTACCGTAAAGGAGATAACTAACATGAATGAAACGTATc          >  1:387217/1‑141 (MQ=255)
                                                                                                                              ggTGGAAAATGGTGAAATTTACCCACATCATCATCCTAAATTTAACATTTCAGAAAAATCATTACTTATTTCTGCAGAAGCAGTAGGGACAGTTGTTTTAGATTACCTTAAAGGAGATAACTAACATGAATGAAACGTATc          >  1:45013/1‑141 (MQ=255)
                                                                                                                                 ggAAAATGGTGAAATTTACCCACATCATCATCCTAAATTTAACATTTCAGAAAAATCATTACTTATTTCTGCAGAAGCAGTAGGGACAGTTGTTTTTTATTTCTTTAAAGGAGATAACTAACAAGACTGAAACGTATcgcg       >  2:37478/1‑141 (MQ=255)
                                                                                                                                 ggAAAATGGTGAAATTTACCCACATCATCATCCTAAATTTAACATTTCAGAAAAATCATTACTTATTTCTGCAGAAGCAGTAGGGACAGTTGTTTTAGATTACCTTAAAGGAGATAACTAACATGAATGAAACGTATcgcg       <  1:552108/141‑1 (MQ=255)
                                                                                                                                      aTGGTGAAATTTACCCACATCATCATCCTAAATTTAACATTTCAGAAAAATCATTACTTATTTCTGCAGAAGCAGTAGGGACAGTTGTTTTAGATTACCTTAAAGGAGATAACTAACATGAATGAAACGTATCGCgggggg  >  1:223510/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                        
GGCTAAGACGTTGAAAGAAGCAAACCTTGATTTTGGTGTTGAAATGTGTGAACCACAACCACCTTCAGAAGACTTTGCATATTATGCTAAAGAACGCCCAAGTGCCTTTATTTATACAGGTGCAGCGGTGGAAAATGGTGAAATTTACCCACATCATCATCCTAAATTTAACATTTCAGAAAAATCATTACTTATTTCTGCAGAAGCAGTAGGGACAGTTGTTTTAGATTACCTTAAAGGAGATAACTAACATGAATGAAACGTATCGCGGGGGC  >  CP000730/116813‑117087

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑