Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,440,085 G→T 100% intergenic (‑45/‑891) cspA ← / → xpaC cold shock protein CspA/5‑bromo‑4‑chloroindolyl phosphate hydrolysis protein XpaC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,440,0850GT100.0% 133.5 / NA 42intergenic (‑45/‑891)cspA/xpaCcold shock protein CspA/5‑bromo‑4‑chloroindolyl phosphate hydrolysis protein XpaC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (24/18);  total (24/18)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTCAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATATGGAATAATCATCTGCTTAACTTGTATTATAGTGCAGTTATTACCAAATTGCAATACAATAATAAAAAATATTCATTTA  >  CP000730/1439955‑1440213
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tGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTCAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCTCATATTACaa                                                                                                                        <  1:20949/141‑1 (MQ=255)
 gTAGGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTCAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATATGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCTCATCTTACaaa                                                                                                                       >  1:410602/1‑141 (MQ=255)
         aCGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTCAGCGTTAAACCATTTAACTGTACCTTGTTTCATGATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCTCATATTACAAAAAATACAt                                                                                                               >  2:378376/1‑141 (MQ=255)
         aCGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTCAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCTCATATTACAAAAAATatct                                                                                                               >  2:281678/1‑138 (MQ=255)
            tCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTCAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCTCATATTACAAAAAATACATGCa                                                                                                            <  1:178459/141‑1 (MQ=255)
                    tcCTTCAACTTCGATAAAGCCGAATCCTTTTTCAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATCCATTCAATATGCTTATTCTCATATTACAAAAAATACATGCAAATTTCAc                                                                                                    <  1:351186/141‑1 (MQ=255)
                        tCAACTTCGATAAAGCCGAATCCTTTTTCAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCTCATATTACAAAAAATACATGCAAATTTCACTCaa                                                                                                <  1:320671/141‑1 (MQ=255)
                         caccTTCGATGAGCCCGAATCCTTTTTCATCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAATATCCATTCAATATGCTTATTCTCATATTACAAAAAATACATGCAAATTTCACTCaaa                                                                                               <  2:557860/138‑1 (MQ=255)
                                 aTAAAGCCGAATCCTTTTTCAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCTCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCttt                                                                                       <  2:507392/141‑1 (MQ=255)
                                    aaGCCGAATCCTTTTTCAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCTCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCa                                                                                    >  2:524908/1‑141 (MQ=255)
                                     aGCCGAATCCTTTTTCAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCTCCTATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCaa                                                                                   >  2:128229/1‑141 (MQ=255)
                                      gCCGAATCCTTTTTCAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACATCCAAGACTAAAATTCATTCAATATGCTTATTCTCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGAAat                                                                                  >  2:497756/1‑141 (MQ=255)
                                        cGAATCCTTTTTCAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCTCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAatat                                                                                <  1:232541/141‑1 (MQ=255)
                                           aTCCTTTTTCAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCTCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATATGGa                                                                             >  2:350781/1‑141 (MQ=255)
                                                ttttCAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCTCATATTACAAAAAATACATGCAAATTTCACTCAAATATCCGTTGCAATATGGaataat                                                                        <  2:123918/141‑1 (MQ=255)
                                                      gCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCTCATATTACAAAAAATACATGCAAATTTCTCTCAAATATTCTTTGCAATATGGAATAATCATCTg                                                                  <  2:375965/141‑1 (MQ=255)
                                                       cGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCTCATATTACAAAAAATACCTGCAAATTTCACTCAAATATTCTTTGCAATATGGAATAATCATCTGc                                                                 <  1:449813/141‑1 (MQ=255)
                                                       accaaaaCCATTTCAACATACCTTTCTTTCTAATCCAAAACCTCCAAGACTAAAATTCATTCAATACGCTTATTCTCATATTACAAAAAATACATGCAAATTTCAGCCAAATATTCTTTGCAATATGGAATAATCATCTGc                                                                 <  2:211501/137‑1 (MQ=255)
                                                        gTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCTCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATATGGAATAATCATCTGCt                                                                >  2:242927/1‑141 (MQ=255)
                                                            aaCCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCTCATATTACAAAAAATACATGCCAATTTCACTCAAATATTCTTTGCAATATGGAATAATCCTCTCCTTAAc                                                            >  1:158456/1‑141 (MQ=255)
                                                              ccATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCTCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATATGGAATAATCATCTGCTTAACtt                                                          >  2:566044/1‑141 (MQ=255)
                                                              ccATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCTCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATATGGAATAATCATCTGCTTAACtt                                                          <  1:314820/141‑1 (MQ=255)
                                                                 tttAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCTCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATTTGGAATAATCATCTGCTTAACTTGta                                                       >  1:70935/1‑141 (MQ=255)
                                                                     aCTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCTCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATATGGAATAATCATCTGCTTAACTTGtattat                                                   >  1:166621/1‑141 (MQ=255)
                                                                     aCTGTACCTTGTTTCATAAACTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCTCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATATGGAATAATCATCTGCTTAACTTGtattat                                                   >  1:426114/1‑141 (MQ=255)
                                                                      cTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCTCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATATGGAATAATCATCTGCTTAACTTGTATtata                                                  <  1:71561/141‑1 (MQ=255)
                                                                        gTACCTTGTTTCATAATCTGAAACCTCCCAGACTAAAATTCATTCAATATGCTTATTCTCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATATGGAATAATCATCTGCTTAACTTGTATTATAGt                                                >  2:50604/1‑141 (MQ=255)
                                                                              tGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCTCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATATGGAATAATCATCTGCTTAACTTGTATTATAGTGCAGtt                                          <  1:505703/141‑1 (MQ=255)
                                                                              tGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCTCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATATGGAATAATCATCTGCTTAACTTGTATTATAGTGCAGtt                                          >  2:329140/1‑141 (MQ=255)
                                                                                 ttCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCTCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATATGGAATAATCATCTGCTTAACTTGTATTATAGTGCAGttatt                                       >  2:204568/1‑141 (MQ=255)
                                                                                   catatgcTGAATCCTCACAGACTAAAATTCATTCAATATGCTTATTCTCATATTACAAAAAATACATGCAAAGTTCACTCAAATATGCTTTGCAATATGGAATAATCATCTGCTTAACTTGTATTATAGTGCAGTTATTAc                                     <  2:339429/135‑1 (MQ=255)
                                                                                     tAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTGTCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATATGGAATAATCATCTGCTTAACTTGTATTATAGTGCAGTTATTACCa                                   >  2:304009/1‑141 (MQ=255)
                                                                                     tAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCTCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATATGGAATAATCATCTGCTTAACTTGTATTATAGTGCAGTTATTACCa                                   >  2:105040/1‑141 (MQ=255)
                                                                                     tAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCTCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATATGGAATAATCATCTGCTTAACTTGTATTATAGTGCAGTTATTACCa                                   >  1:40107/1‑141 (MQ=255)
                                                                                        tCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCTCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATATGGAATAATCATCTGCTTAACTTGTATTATAGTGCAGTTATTACCAAAt                                <  1:352425/141‑1 (MQ=255)
                                                                                                     aGACTAAAATTCATTCAATATGCTTATTCTCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATATGGAATAATCATCTGCTTAACTTGTATTATAGTGCAGTTATTACCAAATTGCAATACaataa                   >  2:197848/1‑141 (MQ=255)
                                                                                                     aGACTAAAATTCATGCAATATGCTTATTCTCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAACATGGAATAATCATCTGCTTAACTTGTATTATAGTGCAGTTATTACCAAATTGcattccattat                   >  2:389809/1‑132 (MQ=255)
                                                                                                          aaaaTTCATTCAATATGCTTATTCTCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATATGTAATAATCATCTGCTTAACTTGTATTATAGTGCAGTTATTACCAAATTGCAATACAATAATaaaa              >  2:456891/1‑141 (MQ=255)
                                                                                                            aaTTCATTCAATATGCTTATTCTCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATATGGAATAATCATCTGCTTAACTTGTATTATAGTGCAGTTATTACCCAATTGCAATACAATAATaaaaaa            >  1:82835/1‑141 (MQ=255)
                                                                                                               gaagacaAAATGCTTATTCTCATATAAAAAAAAAAAAATACAAATTTCACTCAAATATTCCGTGCAATATCGAATAAGCATCTGCTTAACTGGTATTATAGTGCAGTTATTACCAAATTGTAAAAAAAAAAAAAAAAatat         <  2:281607/136‑1 (MQ=255)
                                                                                                                     aaTATGCTTATTCTCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATATGGAATAATCATCTGCTTAACTTGTATTATAGTGCAGTTATTACCAAATTGCAATACAATAATAAAAAATATTCAttt   <  1:524908/141‑1 (MQ=255)
                                                                                                                      atatGCTTATTCTCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATATGGAATAATCATCTGCTTAACTTGTATTATAGTGCAGTTATTACCAAATTGCAATACAATAATAAAAAATATTCATTTa  >  2:524061/1‑141 (MQ=255)
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TGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTCAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATATGGAATAATCATCTGCTTAACTTGTATTATAGTGCAGTTATTACCAAATTGCAATACAATAATAAAAAATATTCATTTA  >  CP000730/1439955‑1440213

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: