Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 76,969 G→A 100% intergenic (+1027/‑107) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073076,9690GA100.0% 142.5 / NA 47intergenic (+1027/‑107)USA300HOU_0072/USA300HOU_0075universal stress protein/lysophospholipase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/24);  total (23/24)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CTTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAGGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAATGCATTATATAAAATTTATTGAGTCAA  >  CP000730/76832‑77103
                                                                                                                                         |                                                                                                                                      
ctTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAGAAGGt                                                                                                                                     >  2:538506/1‑141 (MQ=255)
ctTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGGTAGCTATATTTTTTTACTTTGCAACAGAACCTATCAAAAACATGGGCGTAAAAGGt                                                                                                                                     >  1:57828/1‑141 (MQ=255)
  tCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTa                                                                                                                                   <  1:146495/141‑1 (MQ=255)
     gATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAAtt                                                                                                                                >  1:4784/1‑141 (MQ=255)
     gATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAAtt                                                                                                                                >  2:270844/1‑141 (MQ=255)
       tCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTta                                                                                                                              <  1:481252/141‑1 (MQ=255)
       tCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAAAACCTATAAAAAACATGAGCTTAAAACTTTAAttaa                                                                                                                              >  2:24694/1‑139 (MQ=14)
        cTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTtat                                                                                                                             >  1:178231/1‑141 (MQ=255)
          aCGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATCTTTAAGTACTGTGCAACAGAACCTATAAAAAACATGGACTTAAAAGGTTAAGTtatat                                                                                                                           >  1:442386/1‑141 (MQ=17)
           cgtaTTTTCGCCATGCCACGAAATGAGCAACATGCTAGCAAGTTAAGCGAACACTCACAGTATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTtatata                                                                                                                          <  2:283982/138‑1 (MQ=17)
            ggATTTTCGCCATGCCCTGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAg                                                                                                                         <  2:82947/141‑1 (MQ=255)
            ggATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAg                                                                                                                         <  2:210831/141‑1 (MQ=255)
               ttttCGCCATGCCACGAAATTAGCATCATGGTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTAt                                                                                                                      <  2:72212/141‑1 (MQ=255)
                tttCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTAtt                                                                                                                     <  1:241212/141‑1 (MQ=255)
                          ccACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACa                                                                                                           <  1:239672/141‑1 (MQ=255)
                                     gCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAACCATTGATTTgggg                                                                                                >  2:332188/1‑140 (MQ=255)
                                      aatcatGCTAGCAAGCTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTc                                                                                               <  1:118103/140‑1 (MQ=255)
                                             cTAGCAAGTTAAGCGAACACTGACATGATGAATTAGTGGTTAGCTATATTTTTTTTTTTTTTATCAGAACCTATAAAAAAAATGGGCTTTAAAAGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAAtt                                                                                        >  2:371548/1‑141 (MQ=25)
                                                       aaacaaaCACTGACATGAAAAAAAACTAGTAAGCTAAATTTTATTATTGAGCAAAAAAAAAAAAAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTa                                                                              <  2:428182/136‑1 (MQ=255)
                                                       aaGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTAGGGTCAAAAATTAGGGGTATTa                                                                              >  1:332569/1‑141 (MQ=255)
                                                         gCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAAAATATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAACAATAGATTTGGGTCAAAAATTAGGGGTATTaaa                                                                            >  2:187612/1‑141 (MQ=37)
                                                                cTGACATGATAAATTCGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAaatga                                                                     >  1:423758/1‑141 (MQ=255)
                                                                    cATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCACAAATTAGGGGTATTAAAAAAatgaatga                                                                 >  1:309001/1‑141 (MQ=255)
                                                                       gATAAATTAGTGGTTATCTATACTTTTTTCCTTTGCAACAGAACCGATCAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTACTGGTATGAAAAAAATGAATGAATc                                                              <  1:278010/141‑1 (MQ=255)
                                                                         tAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCAt                                                            <  1:139415/141‑1 (MQ=255)
                                                                            aTTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAAt                                                         >  1:103407/1‑141 (MQ=255)
                                                                               aGTGGATAGCTATATTTTTTTCCTTTTCAATAGAACCTATAAAAAACATGGGGTGAAAAGGTTATCTTAAATAGTGGTACTGCAAACATTGATCGGGTTCAAAAATTAGAAAAAAAAAAAAAATGAATGAATCATAAtttt                                                      <  2:392973/141‑1 (MQ=255)
                                                                                gTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACCTGGGCTTCAAAGTTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAACTTAGGGGTATTAAAAAAAAGAATCAATCATAATTTTa                                                     >  2:271814/1‑141 (MQ=38)
                                                                                  ggTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATc                                                   >  1:230644/1‑141 (MQ=255)
                                                                                               tttttACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGgag                                      >  1:534824/1‑141 (MQ=255)
                                                                                                  ttACGTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAgg                                   <  2:421990/141‑1 (MQ=255)
                                                                                                   tACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGt                                  <  2:423758/141‑1 (MQ=255)
                                                                                                        tGCAACAGAACCTATAAAACCAATAAGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATACTTTTATGCAGCTGATTGGAGAGGTTaaaa                             >  2:123509/1‑141 (MQ=255)
                                                                                                        tGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAAGGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTaaaa                             >  2:379494/1‑141 (MQ=255)
                                                                                                        tGCAACAGAACCTATAAAAAACACGGGCTTAAAAGGTTACTTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGCTTacaa                             >  2:33897/1‑141 (MQ=255)
                                                                                                             acaaaCATAAAAAAAACATGGTCTTAAAAGGTTAATGTACAAAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGCATTAAAAAAATGAATGAATCATAATTTTCTCAAGCTGATTGGAGAGGTTAAAATGCAt                        <  2:5477/138‑1 (MQ=255)
                                                                                                             aaGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAATGCAt                        <  2:545942/140‑1 (MQ=255)
                                                                                                              aGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAAAGCAtt                       >  2:473766/1‑141 (MQ=255)
                                                                                                                   cTATAAAAAACATGGGCTTAACACACTTATTGATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAATGCATtatcta                  >  2:549686/1‑141 (MQ=255)
                                                                                                                   cTATAAAAAACATGGGCTTAAAACGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATTATTTTATCAAGCTGATTGGAGAGGTTAAAATGCATtatata                  <  2:242234/141‑1 (MQ=255)
                                                                                                                     ataAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAATGCATTATATaaa                <  1:567255/141‑1 (MQ=255)
                                                                                                                     ataAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAATGCATTATATaaa                >  1:560484/1‑141 (MQ=255)
                                                                                                                     ataAAAAACAAGGGCTTAAAAGGTTAATTTATATAGTATTACAACAAACATTGATTTGGGTCAAAAATAAAAAAAAAAAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAATGCATTATATaaa                <  2:114458/141‑1 (MQ=255)
                                                                                                                              aTGGGTTTAAAAGGTTAATTTATATAATAATACCGCAAACATTGATTTGGGTCAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAATGCATTATATAAAATTTATTGAg      <  2:252429/141‑1 (MQ=255)
                                                                                                                               tGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAATGCATTATATAAAATTTATTGAg      <  1:42396/141‑1 (MQ=255)
                                                                                                                                  gCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAATGCATTATATAAAATTTATTGAGTCa   <  1:354941/141‑1 (MQ=255)
                                                                                                                                   cTTAAAAGGTTAATTTATATAGTATTACTGCAAACAGTGATTTTGGTCAAAAAATACAGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAATGCATTATATAAAATTTATTGAGTCaa  <  2:332569/141‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                      
CTTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAGGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAATGCATTATATAAAATTTATTGAGTCAA  >  CP000730/76832‑77103

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: