| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP000730 | 1,963,768 | C→G | 100% | V365L (GTA→CTA) | hemG ← | protoporphyrinogen oxidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 1,963,768 | 0 | C | G | 100.0% | 223.6 / ‑6.5 | 66 | V365L (GTA→CTA) | hemG | protoporphyrinogen oxidase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base G (34/30); minor base T (1/1); total (35/31) | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TTCGGCAAACGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACG‑AATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATGGGTATTTTCAATG > CP000730/1963635‑1963901 | ttCGGCAAACTATCGACAATTGAAAAGTCAGTATCACCTTTAAATGTCATCATTTTACTTAAATCTCTACGTACAATCGATACTAATTCAGTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCa < 2:356556/141‑1 (MQ=255) aaCGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCCCG‑AATCa > 1:330443/1‑141 (MQ=255) aCAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACG‑ACTCAAAaccttc > 2:551936/1‑140 (MQ=255) aCAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAacctta > 2:553737/1‑141 (MQ=255) aCAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAacctta > 1:471892/1‑141 (MQ=255) aTTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAaccttacct < 2:456628/141‑1 (MQ=255) tGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTc > 2:323023/1‑141 (MQ=255) aaTTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCggg > 2:437075/1‑140 (MQ=255) aTTCAGGCTCACCTTTAAGTGTCATCATTTGACTTAACTCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGgtg < 1:464887/141‑1 (MQ=255) ttCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACGATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGgtgt < 1:361225/141‑1 (MQ=255) ttCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAATCCTTACCTTCCGgtgt < 2:551133/141‑1 (MQ=255) ttCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATAACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGgtgt > 1:267371/1‑141 (MQ=255) ttCAGGATCACCTCTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGgtgt < 2:427414/141‑1 (MQ=255) cAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGGTgtag > 2:182767/1‑141 (MQ=255) cAGGACCACCTTTAAATTTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGGTgtag < 2:192120/141‑1 (MQ=255) ggATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTGATTCATTCTCTGTATGATCATCAAACACAGTATGACCTCGTGTACATAGATACG‑ACGTAATCAAAACCTTACCGTACggagcaaga > 2:268232/1‑134 (MQ=255) cACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGGTGTAGTAAATg > 2:436128/1‑141 (MQ=255) cccTTTAAACGTAATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGGTGTAGTAAATgg < 2:38411/140‑1 (MQ=255) ccTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGGTGTAGTAAATGGc < 2:372784/141‑1 (MQ=255) ccTTTAAATGTCATCATTTGACTTAAATCTCCACGTACAAGCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGGTGTAGTAAATGGc < 2:507571/141‑1 (MQ=255) cTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGCTACTCATAAATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTTCCTATATACGCACG‑AATCAAAACCGTACCTTCCGGGGTAGTAAATGGcc > 1:474461/1‑141 (MQ=255) ttAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCAt > 2:338635/1‑141 (MQ=255) aaaTGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCAttt < 1:553543/141‑1 (MQ=255) ttcatcaTTTGACTTAAATCTTTACGTACAATCGATACTAATTCATTATCTGTATGATCCTCAACCACATTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCATACCGTCCGGTGTAGTAAAGGGCCATTTTTTc < 2:238133/140‑1 (MQ=255) gTCATCGTGTGACTGAAATCTCTACGTACAACCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTc < 1:411924/141‑1 (MQ=255) gTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCAttttttt > 2:519989/1‑140 (MQ=255) tcatcaGTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCg < 1:3373/141‑1 (MQ=255) catcatTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGa > 1:500030/1‑141 (MQ=255) tcatTTGACTTAAAACTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATg < 1:437075/141‑1 (MQ=255) ccatTTGACTTAAATCTCTGCCCCCAATGCATACTAATTCATTACGTGCATGACCATCAACCACAGTATCACCTGGTTTACCTATATACGCACG‑AATCAAACCCTTACCTTCCGGTGTAGTAAACGGCCTTTTTTTCGATg < 2:557500/140‑1 (MQ=255) acatTTGACTTAAATCACTACGTACACTCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGTTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATg < 1:291924/140‑1 (MQ=255) gACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCACCAACCACAGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAg < 2:287748/141‑1 (MQ=255) aCTTAAATCCCTACGTCCAATCGATACTAAGTCATTATCTGTATGATCATCACCCACAGTATCACCTGGTTTACCTAGATACGCACG‑ACTCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGt < 2:161127/141‑1 (MQ=255) ctctACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCgg > 1:382557/1‑141 (MQ=255) ctACGTATAATCGATANTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTa < 1:480829/141‑1 (MQ=255) tACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTaa > 1:365503/1‑141 (MQ=255) cGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGGGGTAATg > 1:395030/1‑141 (MQ=255) tACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTc > 2:212684/1‑141 (MQ=255) aaTCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGt > 2:36449/1‑141 (MQ=255) aaTCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGt < 1:554491/141‑1 (MQ=255) aaTCGATACTAATTCATTATCTGTATGAACATCAACCACAGTATCACCTGGTTTACCTAGATCCGCACG‑AATCAAAACCTTCCCTACCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGt < 1:198905/141‑1 (MQ=255) atgcTAATTCATTATCTGTATGATCATGAACCACAGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCAc < 2:77528/138‑1 (MQ=255) aTACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCAc < 2:117475/141‑1 (MQ=255) aTACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCAc < 2:380902/141‑1 (MQ=255) tAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTGATGTTTGTATCACTCGt > 1:43384/1‑141 (MQ=255) ttATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCa > 2:384906/1‑141 (MQ=255) tCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAAtt < 1:436128/141‑1 (MQ=255) tGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTAc > 1:459579/1‑141 (MQ=255) tGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATAACTCGTTCTCGCAATTACGAAg > 1:232156/1‑141 (MQ=255) gATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGc > 2:247354/1‑141 (MQ=255) tcatcaACCACAGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTCATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCa < 2:234434/141‑1 (MQ=255) atcaACCACAGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCTCAATTACGAAGCCAGt < 1:338635/141‑1 (MQ=255) atcaACCACAGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGGTGGATTAAATAGCCATTTTTTCGATTTCCAAGTACATGCGGTAATGTCTGTCTCCCTCGTTCTCGCACTTACAAAGCCAGt > 2:162971/1‑141 (MQ=255) ccACAGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACcat > 1:404640/1‑141 (MQ=255) ccACAGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATATCCACGTACATGCGGTAATGTCTGTATCACTCGTTCCCGCAATTACGAAGCCAGTACcac > 2:252121/1‑140 (MQ=255) ccACAGTATCACCTGGTTGACCTAGATACGCACG‑AATCAAATCCTTACCTTCCTGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACGCGTTCTCGCAATTACGAAGCCAGTACcat < 2:71851/141‑1 (MQ=255) cacaGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACcatc > 1:545740/1‑141 (MQ=255) acaGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATACGGTAATGTCTGTATCAATCGTTCTCGCAATTACGAAGCCAGTACcatca > 1:121935/1‑141 (MQ=255) aGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATg > 2:39338/1‑141 (MQ=255) aGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATg > 2:344150/1‑141 (MQ=255) aGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATg > 2:48127/1‑141 (MQ=255) gTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATgg > 1:462531/1‑141 (MQ=255) tCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATGGGTa > 2:199910/1‑141 (MQ=255) cACCTGGTTTACCTAGATACGCACG‑ATGCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACGTGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATGGGTAt < 2:383738/141‑1 (MQ=255) gTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATGGGTATTTTCaa < 1:551936/141‑1 (MQ=255) ttACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATGGGTATTTTCAATg > 2:73094/1‑141 (MQ=255) | TTCGGCAAACGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACG‑AATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATGGGTATTTTCAATG > CP000730/1963635‑1963901 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |