Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,454,626 A→T 100% L48L (CTA→CTT USA300HOU_2315 → hypothetical membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,454,6260AT100.0% 101.9 / ‑6.5 34L48L (CTA→CTTUSA300HOU_2315hypothetical membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (24/9);  minor base G (1/0);  total (25/9)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ACAATAAAATTTCTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTAGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAATTGAACGTCTATTGCTAGCTTTAATTTTACCTTTAATTATT  >  CP000730/2454490‑2454761
                                                                                                                                        |                                                                                                                                       
aCAATAAAATTTGTGGGTTTCAATGGGCAATGACGATTTGCGTATTCTTTGTTAATACACTGGCGGTATCCATTATGCTCAGAGATTTCCGGTCTATAATTGCCGTCCACCACTTTTTATTTTAAGTTACAGATTTTGCAc                                                                                                                                     >  1:75395/1‑141 (MQ=255)
aCAATAAAATTTCTGGTTTTCAATGAGCAATGACGATATTCGGCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGCAGTTAAAGATCTGGCAc                                                                                                                                     >  2:242338/1‑141 (MQ=255)
    tAAAATTTCTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCAtt                                                                                                                                 >  2:194529/1‑141 (MQ=255)
           tCTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAATtgct                                                                                                                          <  1:194529/141‑1 (MQ=255)
              ggTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAATTGCTGCa                                                                                                                       <  1:79059/141‑1 (MQ=255)
                      aTGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAATTGCTGCAATCATTTg                                                                                                               >  2:393866/1‑141 (MQ=255)
                        gggCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAATTGCTGCAATCATTTGta                                                                                                             >  1:492020/1‑141 (MQ=255)
                         ggCAATGACGATTTTCGTCTTCTTTGCACTTTTAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAATTGCTGCAATCATTTGtac                                                                                                            >  2:97225/1‑140 (MQ=255)
                               gACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTCCCCGCCCATAATTGGTGCCAAACACTTTATATTCGAAGTTACCGCTCTTGCCCCCTTCCTCGCCGCAACCCTTTGtctccccgc                                                                                                      >  2:388653/1‑133 (MQ=255)
                                aCGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGTTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAATTGCTGCAATCATTTGTATACTCGtt                                                                                                     >  1:78365/1‑141 (MQ=255)
                                aCGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAATTGCTGCAATCATTTGTATACTCGtt                                                                                                     >  1:515322/1‑141 (MQ=255)
                                           cttcttTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATaa                                                                                          >  1:457873/1‑141 (MQ=255)
                                                     aTTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTCTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAATTGCTGAACTCATTTGTATACTCGTTTTCAAACATAAAAAGGTCCac                                                                                >  2:106187/1‑140 (MQ=255)
                                                       tACAATGGCGTAATCCATTATGCTCAGAGATATCCAGTCTATAATTGGTGTCAAACACTTTATAGTTGAAGTTACAGATCTTGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCTAATATAAAAAGGTCCAACt                                                                              <  1:303375/141‑1 (MQ=255)
                                                        aCAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACtt                                                                             >  1:398706/1‑141 (MQ=255)
                                                        aCAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAATTGCTGCAATCATTTGTATACACGTTTTCAATTATAACAGGGTGCAACtt                                                                             >  1:165778/1‑141 (MQ=255)
                                                          aaTGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGGCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGc                                                                           >  1:302215/1‑141 (MQ=255)
                                                                     ccATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAAt                                                                >  1:552523/1‑141 (MQ=255)
                                                                       aTTATGCTCAGAGATTTCCAATCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATtc                                                              >  1:348256/1‑141 (MQ=255)
                                                                             cTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAAta                                                        >  1:248003/1‑140 (MQ=255)
                                                                                   gaTTTCCAGTCCATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTg                                                  <  1:147358/141‑1 (MQ=255)
                                                                                                taATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAATTGAAc                                     >  1:496243/1‑141 (MQ=255)
                                                                                                       tgtCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAGGTAATTGAACGTCTAtt                              >  1:138638/1‑141 (MQ=255)
                                                                                                               acTTTATATTTGAAGTTACAGATCTTGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAATTGAACGTCTATTGCTAGCtt                      <  1:393866/141‑1 (MQ=255)
                                                                                                                 tttATATTTGAAGTTACAGATCTTGCACCACTAAATGCTCCAATCATTAGTATACTAGTCCTCCCCTATACAACGGCCCAAATTACAGGCTGATAATGCTCAATCATCCAGAAAGTTATCGAACGCCTATTGTTAGCTTTa                    >  2:331667/1‑141 (MQ=255)
                                                                                                                 tttATATTTGAAGCTACAGAGCTTGCACCATTAATTGCTACAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTTCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAATTGAACGTCTATTGCTAGCTTTa                    <  1:191496/141‑1 (MQ=255)
                                                                                                                    atatTTGAAGTTACAGATCTTGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAATTGAACGTCTATTGCTAGCTTTAAtt                 >  1:14143/1‑141 (MQ=255)
                                                                                                                     tatTTGAAGTTACAGATCTTGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAATTGAACGTCTATTGCTAGCTTTAAttt                <  2:78365/141‑1 (MQ=255)
                                                                                                                          gAAGTTACAGATCTTGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAATTGAACGTCTATTGCTAGCTTTAATTTTACCt           >  2:257807/1‑141 (MQ=255)
                                                                                                                             gTTACAGATCTTGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAATTGAACGTCTATTGCTAGCTTTAATTTTACCTTTa        >  1:35137/1‑141 (MQ=255)
                                                                                                                              ttACAGATCTTGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAATTGAACGTCTATTGCTAGCTTTAATTTTACCTTTaa       <  1:258009/141‑1 (MQ=255)
                                                                                                                              gtACAGATCTTGCACCATTAATTTCTGCAATCATTTGTATTCTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAATTGAACGTCTATTGCTAGCTTTAATTTTACCTTTaa       <  2:140501/140‑1 (MQ=255)
                                                                                                                                 cAGATCTTGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAATTGAACGTCTATTGCTAGCTTTAATTTTACCTTTAatta    >  1:416107/1‑141 (MQ=255)
                                                                                                                                   gATCTTGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAATTGAACGTCTATTGCTAGCTTTAATTTTACCTTTAattatt  >  1:228097/1‑141 (MQ=255)
                                                                                                                                        |                                                                                                                                       
ACAATAAAATTTCTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTAGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAATTGAACGTCTATTGCTAGCTTTAATTTTACCTTTAATTATT  >  CP000730/2454490‑2454761

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: