| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP000730 | 2,656,349 | C→A | 10.5% | A53S (GCA→TCA) | USA300HOU_2509 ← | possible lactoylglutatione lyase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 2,656,349 | 0 | C | A | 10.5% | 181.5 / 2.4 | 63 | A53S (GCA→TCA) | USA300HOU_2509 | possible lactoylglutatione lyase |
| Reads supporting (aligned to +/- strand): ref base C (25/30); new base A (2/6); total (27/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.48e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTTCATAATATGTTAATGCGTCATCGTTAGGCACTCTAAAAGACGGTCTTGTAATGGAATTTGTTCCTGCCTGCCCTTTTGTAATATTTGGAAAATCAAAGAACGTCATGTCTGTACCTGCCGAACCTACATCATCTGCAAAAAAAGTATGATACGTATAAATATCATCTTGATTGACTGTCTTTTTAACTAAACGCATGCCTAGTACTTCTGTAAAAAATTTATAATTACGTTCTGCATCATCTGTCATTGCAGTAACATGGTGAATACCTAGTA > CP000730/2656211‑2656486 | gTTCATAATATGTTAATGCGTCATCGTTAGGCACTCTAAAAGACGGTCTTGTAATGGAATTTGTTCCTGCCTGCCCTTTTGTAATATTTGGAAAATCAAAGAACGTCATGTCTGTACCTGCCGAACCTACATCATCTGCaa < 2:356211/141‑1 (MQ=255)gTTCATAATATGTTAATGCGTCATCGTTAGGCACTCTAAAAGACGGTCTTGTAATGGAATTTGTTCCTGCCTGCCCTTTTGTAATATTTGGAAAATCAAAGAACGTCATGTCTGTACCTGCCGAACCTACATCATCTGCaa < 1:267582/141‑1 (MQ=255) ttCATAATATGTTAATGCGTCATCGTTAGGCACTCTAAAAGCCGGTCTTGTAATGGAATTTGTTCATGCCTGCCCTTTTGTAATATTTGGAAAATCAAAGAACGTCATGTCTGTACCTGCCGAACCTACATCATCTGCaaa < 1:228161/141‑1 (MQ=255) cATAATATGTTAATGCGTCATCGTTAGGCACTCTAAAAGACGGTCTTGTAATGGAATTTGTTCCTGCCTGCCCTTTTGTAATATTTGGAAAATCAAAGAACGTCATGTCTGTACCTGCCGAACCTACATCATCTGCaaaaa > 1:536503/1‑141 (MQ=255) atatGTTAATGCGTCATCGTTAGGCACTCTAAAAGACGGTCTTGTAATGGAATTTGTTCCTGCCTGCCCTTTTGTAATATTTGGAAAATCAAAGAACGTCATGTCTGTACCTGCCGAACCTACATCATCTGCAAAAAAAGt < 1:315909/141‑1 (MQ=255) ttAATGCGTCATCGTTAGGCAAAATAAAAGACGGTCTTGTAATGGAATTTGTACCTGCCTGCCCTTTTGTAATATTTGGAAAATCAAATAACGTAATGTTTGTCCCTGCCGAACTTAAAAAAAAAAAAAAAAAAGTATGAt < 2:493595/141‑1 (MQ=255) aTGCGTCATCGTTAGGCACTCTAAAAGACGAACTTGTAATGGAATTTGTTCCTGCCTGCCCTTTTGTAATATTTGGAAAATCAAAGAACGTCATGTCTGTACCTGCCGAACCTACATCAGCTGCAAAAAAAGTATGATACg > 1:136677/1‑141 (MQ=255) tGCGTCATCGTTAGGCACTCTAAAAGACGGTCTTGTAATGGAATTTGTTCCTGCCTGCCCTTTTGTAATATTTGGAAAATCAAAGAACGTCATGTCTGTACCTGCCGAACCTACATCATCTGCAAAAAAAGTATGATACGt > 1:553440/1‑141 (MQ=255) agCGTCAACGATAAGCAATAAAAAAGACGGTTTTGTAATAGAATTTGTTCCCGAAAGCCCTCTTGTAATATTTGGAAAATCAAAGAACATAATGTCTGTACCTGCCGAACCTAAAAAAAAAAAAAAAAAAGTATGATACGt < 2:339527/140‑1 (MQ=255) gCGTCATCGTTAGGCACTCTAAAAGACGGTCTTGTAATGGAATTTGTTCCTGCCTGCCCTTTTGTAATATTTGGAAAATCAATGAACGTCATGTCTGTACCTGCCGAACCTACATCATCTGCAAAAAAAGTATGATACGta < 1:349858/141‑1 (MQ=255) gCGTCATCGTTAGGCACTCTAAAAGACGGTCTTGTAATGGAATTTGTTCCTGCCTGCCCTTTTGTAATATTTGGAAAATCAAAGAACGTCATGTCTGTACCTGCCGAACCTACATCATCTGCAAAAAAAGTATGATACGta > 1:233435/1‑141 (MQ=255) cGTCATCGTTAGGCACTCTAAAAGACGGTCTTGTAATGGAATTTGTTCCTGCCTGCCCTTTTGTAATATTTGGAAAATCAAAGAACGTCATGTCTGTACCTGCCGAACCTACATCATCTGCAAAAAAAGTATGATACGtat < 1:330484/141‑1 (MQ=255) cGTCATCGTTAGGCACTCTAAAAGACGGTCTTGTAATGGAATTTGTTCCTGCCTGCCCTTTTGTAATATTTGGAAAATCAAAGAACGTCATGTCTGTACCTGCCGAACCTACATCATCTGCAAAAAAAGTATGATACGtat > 2:567137/1‑141 (MQ=255) tCATCGTTAGGCACTCTAAAAGACGGTCTTGTAATGGAATTTGTTCCTGCCTGCCCTTTTGTAATATTTGGAAAATCAAAGAACGTCATGTCTGTACCTGCCGAACCTACATCATCTGCAAAAAAAGTATGATACGTATaa > 2:466396/1‑141 (MQ=255) tCGTTAGGCACTCTAAAAGACGGTCTTGTAATGGAATTTGTTCCTGCCTGCCCTTTTGTAATATTTGGAAAATCAAAGAACGTCATGTCTGTACCTGCCGAACCTCCATCATCTGCAAAAAAAGTATGATACGTATAAata > 1:205592/1‑141 (MQ=255) tCGTTAGGCACTCTAAAAGACGGTCTTGTAATGGAATTTGTTCCTGCCTGCCCTTTTGTAATATTTGGAAAATCAAAGAACGTCATGTCTGTACCTGCCGAACCTACATCATCTGCAAAAAAAGTATGATACGTATAAata < 1:32406/141‑1 (MQ=255) cGTTAGGCACTCTAAAAGACGGTCTTGTAATGGAATTTGTTCCTGCCTGCCCTTTTGTAATATTTGGAAAATCAAAGAACGTCATGTCTGTACCTGCCGAACCTACATCATCTGCAAAAAAAGTATGATACGTATAAatat > 2:309767/1‑141 (MQ=255) tAGGCACGCTAAAAGACGGTCTTGTAATGGAATTTGTTCCTGCCTGCCCTTTTGTAATATTTGGAAAAGCAAAGAACGTCATGTCTGTACCTGCCGAACCTACATCATATGCAAAAAAAGTATGATACGTATAAATatcat < 2:486776/141‑1 (MQ=255) tttctAAAAGACGGTTTTGTAATGGAATTTGTTCCTGCCTGCCCTTTTGTAATATTTGGAAAATCAAAGAACGTCATGTCTGTACCTGCCGAACCTACATCGTCTGCAAAAAAAGTATGATACGTATAAATATCATCttga < 1:378614/139‑1 (MQ=255) aataCGTTCTTGTAATCGAATTTGTTCCTACCTGCCCTTTTGTAATATTTGAAAAATCAAAGAACGTCATGTCTGTACCTGCCGAACCTACATCAACTAAAAAAAAAGTATGATACGTATAAATATCATCTTGATTGACTg < 2:254231/138‑1 (MQ=255) aaGACGGTCTTGTAATGGAATTTGTTCCTGCCTGCCCTTTTGTAATATTTGGAAAATCAAAGAACGTCATGTCTGTACCTGCCGAACCTACATCATCTGCAAAAAAAGTATGATACGTATAAATATCATCTTGATTGACTg > 1:125577/1‑141 (MQ=255) aaGACGGTCTTGTAATGGAATTTGTTCCTGCCTGCCCTTTTGTAATATTTGGAAAATCAAAGAACGTCATGTCTGTACCTGCCGAACCTACATCATCTGCAAAAAAAGTATGATACGTATAAATATCATCTTGATTGACTg < 1:569677/141‑1 (MQ=255) gACGGTCTTGTAATGGAATTTGTTCCTGCCTGCCCTTTTGTAATATTTGGAAAATCAAAGAACGTCATGTCTGTACCTGCCGAACCTACATCATCTGCAAAAAAAGTATGATACGTATAAATATCATCTTGATTGACTGTc < 1:201741/141‑1 (MQ=255) ggTCTTGTAATGGAATTTGTTCCTGCCTGCCCTTTTGTAATACTTGGAAAATCAAAGAACGTCATGTCTGTACCTGCCGAACCTAAAAAAAAAGAAAAAAAAGTATGATACGTATAAATATCATCTTGATTGACTGTCttt < 2:188005/141‑1 (MQ=255) gTCTTGTAATGGCATTTGTTCCTGCCTGCCCTTTTGTAATATTTGGAAAATCAAAGAACGTCATGTCTGTACCTGCCGAACCTACATCATCTGCAAAAAAAGTATGATACGTATAAATATCATCTTGATTGACTGTCtttt > 2:539195/1‑141 (MQ=255) gagggAAGTGGTTCCTGCCTGCCCTTTTGTAATATTTGGAAAATCAAAGAACGTCATGTCCGTACCTGCCGAACCTACATCATCTGCAAAAAAAGTATGATACGTATAAATATCATCTTGATTGACTGTCTTTTTAACTaa < 1:444060/138‑1 (MQ=255) aagggAATTTGTTCCTGCCTGCCCTTTTGTAATATTTGGAAAATCAAAGAACGTCATGTCTTTACCTGCCGAACCTACAGCATCTGCAAAAAAAGTATGATACGTATAAATATCATCTTGATTGACTGTCTTTTTAACTaa < 2:232981/138‑1 (MQ=255) aaTGGAATTTGTTCCTGCCTGCCCTTTTGTAATATTTGGAAAATCAAAGAACGTCATGTCTGTACCTGCCGAACCTACATCATCTGCAAAAAAAGTATGATACGTATAAATATCATCTTGATTGACTGTCTTTTTAACTaa < 2:484229/141‑1 (MQ=255) tGGAATTTGTACCTGCCTGCCCTTGTGTAATATTTTGAAAATCAAAGAACGTCATGTCTGTACCTGCCGAACCTACATCATCTGCAAAAAAAGTATGATACGTATAAATATCATCTTGATTGACTGTCTTTTTAACTAAAc < 2:9095/141‑1 (MQ=255) ggAATTTGTTCCTGCCTGCCCTTTTGTAATATTTGGAAAATCAAAGAACGTCATGTCTGTACCTGCCGAACCTACATCATCTGCAAAAAAAGTATGATACGTATAAATATCATCTTGATTGACTGTCTTTTTAACTAAACg < 1:4646/141‑1 (MQ=255) tttGTTCCTGCCTGCCCTTTTGTAATATTTGGAAAATCAAAGAACGTCATGTCTGTACCTGCCGAACCTACATCATCTGCAAAAAAAGTATGATACGTATAAATATCATCTTGATTGACTGTCTTTTTAACTAAACGCATg > 1:86350/1‑141 (MQ=255) ttCCTGCCTGCCCTTTTGTAATATTTGGAAAATCAAAGAACGTCATGACTGTACCTGCCGAACCTACATCATCTGCAAAAAAAGTATGATACGTATAAATATCATCTTGATTGACTGTCTTTTTAACTAAACGCATGCCTa > 2:370160/1‑141 (MQ=255) tCCTGCCTGCCCTTTTGTAATCTTTGGAAAATCAAAGAACGTCATGTCTGTACCTGCCGAACCTACATCATCTGCAAAAAAAGTATGATGCGTATAAATATCATCTTGATTGGCTGTCTTTTTAACTAAACGCATGCCTAg > 1:225349/1‑141 (MQ=255) gccCTTTTGTAATATTTGGAAAATCAAAGAACGTCATGTCTGTACCTGCCGAACCTACATCCTCTGCAAAAAAAGTATGATACGTATACATATCATCTTGATTGACTGTCTTTTTAACTAAACGCATGCCTAGTACttttt > 1:328709/1‑138 (MQ=255) gccCTTTTGTAATATTTGGAAAATCAAAGAACGTCATGTCTGTACCTGCCGAACCTACATCATCTGCAAAAAAAGTATGATACGTATAAATATCATCTTGATTGACTGTCTTTTTAACTAAACGCATGCCTAGTACTTCTg < 2:35804/141‑1 (MQ=255) cccTTTTGTAATATTTGGAAAATCAAAGAAAGTCATGTCTGTACCTGCCGAACCTACATCATCTGCAAAAAAAGTATGATACGTATAAATATCATCTTGATTGACTGTCTTTTTAACTAAACGCATGCCTAGTACTTCTGt < 1:295883/141‑1 (MQ=255) cccTTTGGTAATATTTGGAAAATCAAAGAAGGTCATGTCTGTACCTGACGAACCTACATCATCTGCAAAAAAAGTATGATACGTATAAATATCATCTTGATTGACTGTCTTTTTAACTAAACGCATGCCTAGTACTTCTGt < 2:155725/141‑1 (MQ=255) cTTTTGTAATATTTGGAAAATCAAAGAACGTCATGTCTGTACCTGCCGAACCTACATCATCTGCAAAAAAAGTATGATACGTATAAATATCATCTTGATTGACGGTCTTTTTAACTAAAAGCATGCCTAGTACTTCTGTaa > 1:425334/1‑141 (MQ=255) tttGTAATATTTGGAAAATCAAAGAACGTCATGTCTGTACCTGCCGAACCTACATCATCTGCAAAAAAAGTATGATACGTATAAATATCATCTTGATTGACTGTCTTTTTAACTAAACGCATGCCTAGTACTTCTGTaaaa < 2:233435/141‑1 (MQ=255) ttGTAATATTTGGAAAATCAAAGAACGTCATGTCTGTACCTGCCGAACCTACATCATCTGCAAAAAAAGTATGATACGTATAAATATCATCTTGATTGACTGTCTTTTTAACTAAACGCATGCTTAGTACTTCTGTaaaaa > 2:488279/1‑141 (MQ=255) ttGTAATATTTGGAAAATCAAAGAACGTCATGTCTGTACCTGCCGAACCTACATCATCTGCAAAAAAAGTATGATACGTATAAATATCATCTTGATTGACTGTCTTTTTAACTAAACGCATGCCTAGTACTTCTGTaaaaa > 1:558084/1‑141 (MQ=255) tAATATTTGGAAAATCAAAGAACGTCATGTCTGTACCTGCCGAACCTACATCATCTGCAAAAAAAGTATGATACGTATAAATATCATCTTGATTGACTGTCTTTTTAACTAAACGCATGCCTAGTACTTCTGTAAAAAAtt > 2:292240/1‑141 (MQ=255) tAATATTTGGAAAATCAAAGAACGTCATGTCTGTACCTGCCGAACCTACATCATCTGCAAAAAAAGTATGATACGTATAAATATCATCTTGATTGACTGTCTTTTTAACTAAACGCATGCCTAGTACTTCTGTAAAAAAtt < 1:198070/141‑1 (MQ=255) atatTTGGAAAATCAAAGAACGTCATGTCTGTACCTGCCGAACCTACATCATCTGAAAAAAAAGTATGATACGTATAAATATCATCTTGATTGACTGTCTTTTTAACTAAAAGACTACCTAGAACTTCTGTCAAAAAtttt > 1:199261/1‑140 (MQ=255) ttGGAAAATCAAAGAACGTCATGTCTGTACCTGCCGAACCTACATCATCTGCAAAAAAAGTATGATACGTATAAATATCATCTTGATTGACTGTCTTTTTAACTAAACGCATGCCTAGTACTTCTGTAAAAAATTTATAAt < 2:239715/141‑1 (MQ=255) aaaGAACGTCATGTCTGTACCTGCCGAACCTACATCATCTGCAAAAAAAGTATGATACGTATAAATATCATCTTGATTGACTGTCTTTTTAACTAAACGCATGCCTAGTACTTCTGTAAAAAATTTATAATTACGTTCTGc < 1:287927/141‑1 (MQ=255) aaaGAACGTCATGACTGTACCTGCCCAACCTACATCATATGCAAAAAAAGTATGATACGTCTAACTATCATATAGAGTGCCTGTCTTTTTATCTAAACGCCTGCCTAGTACTTCTGTAAAAAATGTATAATTACGTTCTGc > 2:79316/1‑141 (MQ=255) aGAACGTCATGTCTGTACCTGCCGAACCTACATCATCTGCAAAAAAAGTATGATACGTATAAATATCATCTTGATTGACTGTCTTTTTAACTAAACGCATGCCTAGTACTTCTGTAAAAAATTTATAATTACGTTCTGcat > 2:277898/1‑141 (MQ=255) gAACGTCATGTCTGTACCTGCCGAACCTACATCATCTGCAAAAAAAGTATGATACGTATAAATATCATCTTGATTGACTGTCTTTTTAACTAAACGCATGCCTAGTACTTCTGTAAAAAATTTATAATTACGTTCTGcatc > 2:376819/1‑141 (MQ=255) aaCGTCATGTCTGTACCTGCCGAACCTACATCATCTGAAAAAAAAGTATGATACGTATAAATATCATCTTGATTGACTGTCTTTTTAACTAAACGCATGCCTCGTACTTCTGTAACCACGTTAGAATTACGTTATAcatcc > 2:116747/1‑140 (MQ=255) ttCATGTCTGTACCTGCCGAACCTAAATAATATGAAAATAAAGTATGATACGTATGAATATCATCTTGATTGACTGTCTTTTTAACTAAACGCATGCCTAGTACTTCTGTAAAAAATTTATAATTACGTTCTGCATCATCt < 2:126596/140‑1 (MQ=255) ctCATGTCTGTACCTGCCGCTCCGACAGCATCTTCAAAAAAAGTATGATACGTATAAATATCATCTTGATTGACTGTCTTTTTAACTAAACGCATGCCTAGTACTTCTGTAAAAAATTTATAATTACGTTCTGCATCATCt < 1:528490/140‑1 (MQ=255) tCATGTCTGTACCTGCCGAACCTACATCATCTGCAAAAAAAGTATGATACGTATAAATATCATCTTGATTGACTGTCTTTTTAACTAAACGCAGGCCTAGTACTTCTGTAAAAAATTTATAATTACGTTCTGCATCATCTg < 1:190956/141‑1 (MQ=255) cATGTCTGTACCTGCCGAACCTACATCCTCTGCAAAAAAAGTATGATCCGTATAAATATCATCTTGATTTACTGTATTTTTAACTAAACGCATGCCTAGTACTTCTCTAAGAATTTCATAATTACGTTCTggacactctgt > 2:129216/1‑131 (MQ=255) aTGTCTGTACCTGCCGAACCTACATCATCTGCAAAAAAAGTATGATACGTATAAATATCATCTTGATTGACTGTCTTTTTAACTAAACGCATGCCTAGTACTTCTGTAAAAAATTTATAATTACGTTCTGCATCATCTGTc > 2:302964/1‑141 (MQ=255) tACCTGCCGAACCTACATCATCTGCAAAAAAAGTATGATACGTATAAATATCATCTTGATTGACTGTCTTTTTAACTAAACGCATGCCTAGTACTTCTGTAAAAAATTTATAATTACGTTCTGCATCATCTGTCATTGCAg < 1:126703/141‑1 (MQ=255) tGCCGAACCTACATCATCTGCAAAAAAAGTAGGATACGTATAAATATCATCTTGATTGACTGTCTTTTTAACTAAACGCATGCCTAGTACTTCTGTAAAAAATTTATAATTACGTTCTGCATCATCTGTCATTGCAGTAAc < 2:220201/141‑1 (MQ=255) tcatcTGCAAAAAAAGTATGATACGTATAAATATCATCTTGATTGACTGTCTTTTTAACTAAACGCATGCCTAGTACTTCTGTAAAAAATTTATAATTACGTTCTGCATCATCTGTCATTGCAGTAACATGGTGAATACCt > 1:171997/1‑141 (MQ=255) tcatcTGCAAAAAAAGTATGATACGTATAAATATCATCTTGATTGACTGTCTTTTTAACTAAACGCATGCCTAGTACTTCTGTAAAAAATTTATAATTACGTTCTGCATCATCTGTCATTGCAGTAAAATGGTGAATACCt > 1:254163/1‑141 (MQ=255) ctcTGCAAAAAAAGTATGATACGTATAAATATCATCTTGATTGACTGTCTTTTTAACTAAACGCATGCCTAGTACTTCTGTAAAAAATTTATAATTACGTTCTGCATCATCTGTCATTGCAGTAACATGGTGAATACCTAg < 1:570270/140‑1 (MQ=255) atcTGAAAAAAAAGTATGATACGTATAAATATCATCTTGATTGACTGTCCTTTTAACTAAACGCATGCCTAGTACTTCTGTAAAAAATTTATAATTACGTTCTGCATCATCTGTCATTGCAGTAACATGGTGAATACCTAg < 2:536503/141‑1 (MQ=255) tcTGCAAAAAAAGTATGATACGTATAAATATCATCTTGATTGACTGTCTTTTTAACTAACCGCATGCCTAGTACTTCTGTAAAAAATTTATAATTACGTTCTGCATCATCTGTCATTGCAGTAACATGGTGAATACCTAGt < 2:205592/141‑1 (MQ=255) cTGCAAAAAAAGTATGATACGTATAAATATCATCTTGATTGACTGTCTTTTTAACTAAACGCATGCCTAGTACTTCTGTAAAAAATTTATAATTACGTTCTGCATCATCTGTCATTGCAGTAACATGGTGAATACCTAGTa < 2:86350/141‑1 (MQ=255) | GTTCATAATATGTTAATGCGTCATCGTTAGGCACTCTAAAAGACGGTCTTGTAATGGAATTTGTTCCTGCCTGCCCTTTTGTAATATTTGGAAAATCAAAGAACGTCATGTCTGTACCTGCCGAACCTACATCATCTGCAAAAAAAGTATGATACGTATAAATATCATCTTGATTGACTGTCTTTTTAACTAAACGCATGCCTAGTACTTCTGTAAAAAATTTATAATTACGTTCTGCATCATCTGTCATTGCAGTAACATGGTGAATACCTAGTA > CP000730/2656211‑2656486 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |