Predicted mutation
evidence seq id position mutation freq annotation gene description
JC CP000730 2,501,544 Δ108 bp 9.8% coding (68‑175/2106 nt) USA300HOU_2364 → AraC transcriptional regulator

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP000730 = 250154345 (0.700)5 (0.080) 3/240 NT 9.8% coding (67/2106 nt) USA300HOU_2364 AraC transcriptional regulator
?CP000730 2501652 = 50 (0.840)coding (176/2106 nt) USA300HOU_2364 AraC transcriptional regulator

TACTATCCATGATTTTAAAAATTTCCATGTTTTACATTTAAATGATAGATTTCAATATATATTAGGCAGGAGAGAGAAATATGACATGCCAACTTAAAATACATCATACATTATCGACAATACCAAGTCGTAATATTAATAATATTA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  CP000730/2501397‑2501543
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ataatattaACCACGGCGAAAATATAATCGAATTAATGATACCCGTTTTTTATTTTTATCAACAAGATGATGATTTCTTTAATGGTTATTTAGATCGTCATTTATTACAATCAAGTAATTATATTAAGTCATTAATTGCCGATT  >  CP000730/2501652‑2501786
                                                                                                                                                                                                                                                                                          
TACTATCCATGATTTTAAAAATTTCCATGTTTTACATTTAAATGATAGATTTCAATATATATTAGGCAGGAGAGAGAAATATGACATGCCAACTTAAAATACATCATACATTATCGACAATACCAAGTCGTAATATTAAT                                                                                                                                                <  2:643049/140‑1
 aaTATCCAAGATTTTAATAATTTCGAGGTTTTACATTTAAATGATAGATTTCAATATATATTAGGCAGGAGAGAGAAATATGACATGCCAACTTAAAATACATCATACATTATCGACAATACCAAGTCGTAATATTAATA                                                                                                                                               <  1:706216/138‑1
 ACTATCCATGATTTTAAAAATTTCCATGTTTTACATTTAAATGATAGATTTCAATATATATTAGGCAGGAGAGAGAAATATGACATGCCAACTTAAAATACATCATACATTATCGACAATACCAAGTCGTAATATTAATA                                                                                                                                               <  1:619385/140‑1
    ATCCATGATTTTAAAAATTCTCTAGTTCTACATTTAAATGATAGATTTCAATATATATTAGGCAGGAGAGAGAAATATGACATGCCAACTTAAAATACATCATACATTATCGACAATACCAAGTCGTAATATTAATAATA                                                                                                                                            <  1:734485/140‑1
     TCCATGATTTTAAAATTTTCCATGTTTTACATTTAAATGATAGATTTCAATATATATTAGGCAGGAGAGAGAAATATGACATGCCAACTTAAAATACATCATACATTATCGACAATACCAAGTCGTAATATTAATAATAT                                                                                                                                           >  1:383107/1‑140
     TCCATGATTTTAAAAATTTCCATGTTTTACATTTAAATGATGGCTTTCGAAGGATATTAGGCAGGAGAGAGAAATATGACATGCCAACTTAAAATACATCATACATTATCGACAATACCAAGTCGTAATATTAATAATAT                                                                                                                                           >  1:593578/1‑140
     TCCATGATTTTAAAAATTTCCATGTTTTACATTTAAATGATAGATTTCAATATATATTAGGCAGGAGAGAGAAATATGACATGCCAACTTAAAATACATCATACATTATCGACAATACCAAGTCGTAATATTAATAATAT                                                                                                                                           >  1:99695/1‑140
                 AAAATTTCCATGTTTTACATTTAAATGATAGATTTCAATATATATTAGGCAGGGGAGAGAAATATGACATGCCAACTTAAAATACATCATACATTATCGACAATACCAAGTGGTAATATTAATAATATTAACCACAGCaa                                                                                                                               >  2:379239/1‑138
                                                                                   ACATGCCAACTTAAAATACATCATACATTATCGACAATACCAAGTCGTAATATTAATAATATTAACCACGGCGAAAATATAATCGAATTAATGAAAACCGTTTTTTATTTTTATCAACAAGATGATGATTTCTTTAATGG                                                             >  1:317044/1‑140
                                                                                       gctAACTTAAAATACATCATACATTATCGACAATACCAAGTCGTAATATTAATAATATTAACCACGGCGAAAATATAATCGAATTAATGATACCCGTTTTTTATTTTTATCAACAAGATGATGATTTCTTTAATTGTTAT                                                         <  2:383107/137‑1
                                                                                       actAACTTAATATACAACATACATTAGCTACAAGACCAAGTCGTAATATTAATAATATTAACCACGGCGAAAATATAATCGAATTAATGATACCCGTTTTTTATTTTTATCAACAAGATGATGATTTCTTTAATGGTTAT                                                         <  2:593578/137‑1
                                                                                       GCCAACTTAAAATACATCATACATTATCGACAATACCAAGTCGTAATATTAATAATATTAACCACGGCGAAAATATAATCGAATTAATGATACCCGTTTTTTATTTTTATCAACAAGATGATGATTTCTTTAATGGTTAT                                                         <  2:99695/140‑1
                                                                                                                                           TAATATTAACCACGGCGAAAATATAATCGAATTAATGATACCCGTTTTTTATTTTTATTAACAAGATGATGATTTCTTTAATTGTTATTTAGATCGTCATTTATTACAATCAATTAATTATATGAATTCATTAATTGCCa     >  2:75380/1‑139
                                                                                                                                           TAATATTAACCACGGCGAAAATATAATCGAATTAATGATACCCGTTTTTTATTTTTATCAACAAGATGATGATTTCTTTAATTGTTATTTAGATCGTCATTTATTACAATCAAGTAATTATATTAAGTCATTAATTGCCt     >  2:485232/1‑139
                                                                                                                                              TATTAACCACGGCTAATATATAATCGAATTAATGTTACCCGTTTTTTATTTTTATCAACAAGATGATGATTTCTTTAATGGTTATTTAGATCGTCATTTATTACAATCAAGTAATTATATTAAGTCATTAATTGCCGATT  <  2:434256/140‑1
                                                                                                                                                                                                                                                                                          
TACTATCCATGATTTTAAAAATTTCCATGTTTTACATTTAAATGATAGATTTCAATATATATTAGGCAGGAGAGAGAAATATGACATGCCAACTTAAAATACATCATACATTATCGACAATACCAAGTCGTAATATTAATAATATTA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  CP000730/2501397‑2501543
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ataatattaACCACGGCGAAAATATAATCGAATTAATGATACCCGTTTTTTATTTTTATCAACAAGATGATGATTTCTTTAATGGTTATTTAGATCGTCATTTATTACAATCAAGTAATTATATTAAGTCATTAATTGCCGATT  >  CP000730/2501652‑2501786

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.