Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,513,930 A→T 100% L413F (TTA→TTT USA300HOU_1379 → hypothetical membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,513,9300AT100.0% 149.5 / ‑6.5 55L413F (TTA→TTTUSA300HOU_1379hypothetical membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (21/33);  minor base C (0/1);  total (21/34)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GTATGTATCGCTTTATTAGTAGCCATTGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTAATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGCTAAACAATAATGCACTTAAAGTTTTGAACTGACGAAATTTACAA  >  CP000730/1513802‑1514066
                                                                                                                                |                                                                                                                                        
gtatgtatCGCTTTATTAGTAGCCATTGGCATGGGGTTTGTTCCATGCATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAgg                                                                                                                               <  1:709927/140‑1 (MQ=255)
  ttgtatCGTTTTATTAGTAGACATTGGAATGGGTTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATTGCGTAAAAAAACCTGCATCTATATCACTCATAATAGGTTTTATTGTATTAGGat                                                                                                                             <  2:350411/139‑1 (MQ=255)
     tatCGCTTGATTAGTAGCCATTGGCATGGGGTTTGTCACTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGattat                                                                                                                          <  2:362757/140‑1 (MQ=255)
       tCGCTTTATTAGTAGCCATTGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATCCTGCAATAAAAGTACCACATCGCGTACAAAAACCAGCATCTATATCACTCAAAATATGTTTTATTGTATTAGGAttatta                                                                                                                        >  2:402022/1‑140 (MQ=255)
           tttATTAGTAGCCATTGGCATGGGGTCTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGc                                                                                                                    >  2:471736/1‑140 (MQ=255)
             tattaGTAGCCATTGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTCTTAAttgca                                                                                                                  >  2:77262/1‑136 (MQ=255)
               ttaGTAGCCATTGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATATTTTATTGTATTAGGATTATTAATGCCatt                                                                                                                >  1:107063/1‑140 (MQ=255)
                 aggagCCATTGGCATGGGGTTTGGCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCattat                                                                                                              <  2:4735/137‑1 (MQ=255)
                    agCCATTGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAGTGCCATTATTTg                                                                                                           >  2:391132/1‑140 (MQ=255)
                     gCCATTGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTgg                                                                                                          <  1:702270/140‑1 (MQ=255)
                     gCCATTGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATATCTTTTTCGTATGGTTTGTTTCATTGTATTAGGATTATTAATGCGATTATTTgg                                                                                                          <  1:557106/140‑1 (MQ=255)
                      ccATTGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATTTCATTATTTTGa                                                                                                         >  2:491834/1‑140 (MQ=255)
                      ccATTGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATAATTTGGa                                                                                                         >  2:520239/1‑140 (MQ=255)
                       cATTGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCAATCAAAATAGATTTTATTGTATTAGGATTATTAATGCCATTATTTGGAt                                                                                                        >  1:626021/1‑140 (MQ=255)
                          tGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTAt                                                                                                     <  1:385620/140‑1 (MQ=255)
                               tGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAACAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACtt                                                                                                >  1:521460/1‑140 (MQ=255)
                               tGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATNTGTTTTATTGTATTAGGATTANTAATGCCNTTATTTGGATTATCACtn                                                                                                >  1:691149/1‑139 (MQ=255)
                               tGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATGGTATTAGGATTATTAATGCCATTATTTGGATTATCACtt                                                                                                >  2:386609/1‑140 (MQ=255)
                                ggggCTTGTCACTTGGATAACGCGTACAAAAAATACCGCCATAAAAGTACCACATCGCGTAAACAAACCAGCATCTATCTCACTCATAATATGTTTTATAGTATTAAAATTATATAACCCATTATTCGGATCATGACtca                                                                                               >  2:726120/1‑138 (MQ=255)
                                         ctctTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTAt                                                                                      <  1:519017/140‑1 (MQ=255)
                                          tctTGGATAAGGTATAAGAAAAAGTCGGCAATAAAAGTACCATCTCGAGGAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATGATTTGGATTATCACTTATCCTTGTAtt                                                                                     <  2:498088/140‑1 (MQ=255)
                                               gaaaaaGCGGACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATaa                                                                                <  2:215136/137‑1 (MQ=255)
                                                aTAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAAt                                                                               <  1:467279/140‑1 (MQ=255)
                                                  aaaGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACACATAATATGTATTATTGTATTAGAATTATTAATTCCATTATTTGGATTATCACTTGTCCTTATATTTATACTTg                                                                             >  1:1756/1‑140 (MQ=255)
                                                   aaGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTATTAATTGa                                                                            >  1:426953/1‑140 (MQ=255)
                                                   aaGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGAATTATCACTTATCCTTGTATTTATAATTGa                                                                            >  2:64024/1‑140 (MQ=255)
                                                     acGTACAAAAAATACTGCAATAAAAGTAACAAATAAAAAAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAAt                                                                          <  2:534540/139‑1 (MQ=255)
                                                      cGTACAAAAAATACTGCAATAAAAGTACTACATCGCGTAAAAAAACCAGCTTCTATATGTCTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAAtt                                                                         <  1:266266/140‑1 (MQ=255)
                                                            aaaaaTACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAAtatt                                                                   <  1:690348/140‑1 (MQ=255)
                                                            aaaaaTACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGACATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAGTTAAtatt                                                                   >  2:396789/1‑140 (MQ=255)
                                                             aaaaTACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAAtatta                                                                  <  1:379950/140‑1 (MQ=255)
                                                              aaaTACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCATTGAATTTATAATTGAATTAAtattat                                                                 >  1:57420/1‑140 (MQ=255)
                                                                  aCTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTatatat                                                             >  2:99308/1‑140 (MQ=255)
                                                                       aaTAAAAGTACCACATCGCGTAAAAATGCCAGCATCTATATCTCTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGCTCATCTCGTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAg                                                        <  1:400487/140‑1 (MQ=255)
                                                                       aaTAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAg                                                        <  1:749132/140‑1 (MQ=255)
                                                                        aTAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGa                                                       <  2:614376/140‑1 (MQ=255)
                                                                            acgTACAACATCGCGTAAAAAAACCAGCTTCTATATAAATCATAATATTTTTTATTGTATTAGGATTACTAATGCCATTAAGTGGATTATCACTTATCCTTGTAGTTATAATTGAATTAATATTATATATTAAAGAtcgt                                                   <  1:125057/138‑1 (MQ=255)
                                                                               tACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATcgtcgt                                                >  2:297627/1‑140 (MQ=255)
                                                                                agtacaTCGAGTAAAAAAATCAGCATTTATGTCACTCATAATATGTTTTATTGTATTAGGATTATGAATGTCATTAGTTGGATTATTACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTg                                               <  2:309998/137‑1 (MQ=255)
                                                                                aCCACATTGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTg                                               <  2:530149/140‑1 (MQ=255)
                                                                                aCCACATCGTGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATCTATTAAAGATCGTCGTg                                               <  2:657620/140‑1 (MQ=255)
                                                                                aCCACATCGCGTAAAAAAACCCGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTg                                               <  2:214228/140‑1 (MQ=255)
                                                                                aCCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTg                                               <  2:661067/140‑1 (MQ=255)
                                                                                aCCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTg                                               <  1:760997/140‑1 (MQ=255)
                                                                                aCCAAATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTg                                               <  1:450200/140‑1 (MQ=255)
                                                                                               aaaaCCAGCATCTATATCACTCATAATATGTTGTATTTTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAATATCGTCGTGCTAAACAATAATGCa                                <  2:268986/140‑1 (MQ=255)
                                                                                                   ccAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGCTAAACAATAATGCACTTa                            <  1:761591/140‑1 (MQ=255)
                                                                                                            atatCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGCTAAACAATAATGCACTTAAAGTTTTGa                   >  2:648115/1‑140 (MQ=255)
                                                                                                             tatCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAAAATTATATATTAAAGATCGTCGTGCTAAACAATAATGCACGAAAAGATTTaaa                  >  2:25777/1‑140 (MQ=255)
                                                                                                               tCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGCTAAACAATAATGCACTTAAAGTTTTGAACt                <  1:471736/140‑1 (MQ=255)
                                                                                                                  cTCATAATATGTTTTATCGTATTAGGAATATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGCTAAACAATAATGCACTTAAAGTTTTGAACTGAc             <  1:474108/140‑1 (MQ=255)
                                                                                                                     attatatGTTTTATTGTATTAGAGTTATTAATGCCATTATGTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGCTAAACAATAATGCACTTTAAGTTTTGTTCTGACGaa          <  2:513064/137‑1 (MQ=255)
                                                                                                                     ataataTGTTTTATTGTATTAGGATTATTAATGTCATTATTTGGATTATCATTTATCCTTGTATTTATAATTGAATTCTTCTTATACATTAAAGATCGTCGTGCTAAACAATAATGCACTTAAAGTTTTGAACTGACGaa          <  1:330082/140‑1 (MQ=255)
                                                                                                                     ataataTGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGCTAAACAATAATGCACTTAAAGTTTTGAACTGACGaa          <  2:561591/140‑1 (MQ=255)
                                                                                                                     ataataTGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGCTAAACAATAATGCACTTAAAGTTTTGAACTGACGaa          <  1:278147/140‑1 (MQ=255)
                                                                                                                             ttttATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATTGTAGTGGTAAATAATAATGAACTTAAAGTTTTGAAATGAAGAAATTTACaa  >  1:337001/1‑140 (MQ=255)
                                                                                                                                |                                                                                                                                        
GTATGTATCGCTTTATTAGTAGCCATTGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTAATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGCTAAACAATAATGCACTTAAAGTTTTGAACTGACGAAATTTACAA  >  CP000730/1513802‑1514066

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: