Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	1,761,889	C→A	100%	D388Y (GAT→TAT)	folC ←	tetrahydrofolate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,761,889	0	C	A	100.0%	125.3 / NA	42	D388Y (GAT→TAT)	folC	tetrahydrofolate synthase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (22/20);  total (22/20)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TCTTCATGGCTATTTTAGTTTTAGTTTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATCTACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCATCTCGTTAATCGGT  >  CP000730/1761753‑1762022                                                                                                                                         |                                                                                                                                      tcttcATGGCTATTTTAGTTTTAGTTTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTACTAACTAATGTATCTCCTTGATAATTTTTTATAAATTCAACGTAATAATatac                                                                                                                                    >  2:67668/1‑140 (MQ=255) tcttcATGGCTATTTTAGTTTTAGTTTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAATTTCAACGTAGTCATatac                                                                                                                                    >  2:328579/1‑140 (MQ=255) tcttcATGGCTATTTTAGTTTTAGTTTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATatac                                                                                                                                    <  1:258783/140‑1 (MQ=255)   ttcATGGCTATTTTAGTTTTAGTTTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATatacta                                                                                                                                  >  2:97463/1‑140 (MQ=255)   ttcATGGCTATTTTAGTTTTAGTTTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATatacta                                                                                                                                  >  2:671716/1‑140 (MQ=255)    tcATGGCTATTTTAGTTTTAGTTTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAAAGTAGTCATATACTaa                                                                                                                                 >  1:554671/1‑140 (MQ=255)      attgCTATTTTAGTTTTAGTTTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAAtt                                                                                                                               <  2:144391/137‑1 (MQ=255)      aTGGCTATTTTAGTTTTAGTTTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCAACTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAAtt                                                                                                                               <  1:25517/140‑1 (MQ=255)       tGGCTATTTTAGTTTTAGTTTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCCCTTATGAAATACAGACTACCGGTAATTACTAATGTATCACATTGATAATTTTTTATAAATTCTACGTAGTCATATACTAATTg                                                                                                                              >  2:83301/1‑140 (MQ=255)       tGGCTATTTTAGTTTTAGTTTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAAAGTAGTCATATACTAATTg                                                                                                                              >  2:62696/1‑140 (MQ=255)           ttttttagttttagTTTTATAATTCTTCAAAATCCAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGCATCACCTTGATAATTTTTTATAAATTCAACGTAGTCAtatagtcaatgtctt                                                                                                                          <  2:181778/138‑12 (MQ=255)                    tttagttttaTAATTCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCAttt                                                                                                                 <  2:288388/140‑1 (MQ=255)                          tttaTAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCTTCATTTTTAAta                                                                                                           <  1:536946/140‑1 (MQ=255)                            tataATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAAtact                                                                                                         >  1:476951/1‑140 (MQ=255)                                aTGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATActacct                                                                                                     >  2:117866/1‑140 (MQ=255)                                             tAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTcttctt                                                                                        <  1:468738/140‑1 (MQ=255)                                                       ttAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGc                                                                              >  2:348349/1‑140 (MQ=255)                                                        tAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTAATAGCAAGTTCTTCTTTGCGTAACGCt                                                                             <  1:181454/140‑1 (MQ=255)                                                         aaCTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCtt                                                                            <  1:588257/140‑1 (MQ=255)                                                         aaCTTCACGTATGATGTACAGACTACCGGTAATTACTTATGTCTCACCTTGATTATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCGACAATTTTTTCTTTGCGTAACGCtt                                                                            <  2:491654/140‑1 (MQ=255)                                                             tCACTTATGAAATACAGACTATCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCgg                                                                        <  1:443249/140‑1 (MQ=255)                                                                 ttATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTCTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGaaaa                                                                    >  1:714084/1‑140 (MQ=255)                                                                  tATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAAt                                                                   >  1:470547/1‑140 (MQ=255)                                                                      aaaTACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTAAAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCaaa                                                               <  2:559237/140‑1 (MQ=255)                                                                            aGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGt                                                         >  2:108700/1‑140 (MQ=255)                                                                            aGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGt                                                         >  2:714932/1‑140 (MQ=255)                                                                             gACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGtt                                                        >  2:450718/1‑140 (MQ=255)                                                                              aCTACCGGTAATTAGTAATCTATCAGCTTGATAATTTTTTATAAGTTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTg                                                       >  1:415997/1‑140 (MQ=255)                                                                               cTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATAAAATTCAGTGGc                                                      >  1:664358/1‑140 (MQ=255)                                                                               cTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATAATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGc                                                      >  1:61472/1‑140 (MQ=255)                                                                                 aCCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATATTATCTATAATTTCTTCTTTGAGTAACGCTTTCGGAAAATCAAATTCAGTTGTAt                                                    >  1:256228/1‑140 (MQ=255)                                                                                    ggTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATaaa                                                 >  2:67338/1‑140 (MQ=255)                                                                                        cttACTAATGTAGCACCTTGATAATTTTTTATAAATTCAACTTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCGTTGCGTAACGCGTTCGGAAAATCAAATTCAGTTGCATAAAACGt                                             <  1:71731/139‑1 (MQ=255)                                                                                          tACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTAt                                           <  1:37038/140‑1 (MQ=255)                                                                                              aagttaacACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATgcgc                                       <  2:372553/133‑1 (MQ=255)                                                                                              aaggTATCATCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATgcgc                                       <  1:355008/137‑1 (MQ=255)                                                                                                   aTCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTa                                  >  1:370091/1‑140 (MQ=255)                                                                                                         ttGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTa                            <  1:117866/140‑1 (MQ=255)                                                                                                          tGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCGACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTaa                           >  1:527997/1‑140 (MQ=255)                                                                                                           gATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTaaa                          >  1:627727/1‑140 (MQ=255)                                                                                                             tAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATg                        <  2:138900/140‑1 (MQ=255)                                                                                                              aaTTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGt                       <  1:348349/140‑1 (MQ=255)                                                                                                                 tttttATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTg                    >  2:63329/1‑140 (MQ=255)                                                                                                                    ttATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGAcc                 >  2:199013/1‑140 (MQ=255)                                                                                                                         aaTTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCAtctc            >  1:675837/1‑140 (MQ=255)                                                                                                                                   aGTCATATACTATTTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCATCTCGTTAATCGGt  <  1:67668/140‑1 (MQ=255)                                                                                                                                   aGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCATCTCGTTAATCGGt  <  1:328579/140‑1 (MQ=255)                                                                                                                                   aGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCATCTCGTTAATCGGt  <  2:476951/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                      TCTTCATGGCTATTTTAGTTTTAGTTTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATCTACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCATCTCGTTAATCGGT  >  CP000730/1761753‑1762022

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑