Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 76,969 G→A 100% intergenic (+1027/‑107) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073076,9690GA100.0% 100.5 / NA 36intergenic (+1027/‑107)USA300HOU_0072/USA300HOU_0075universal stress protein/lysophospholipase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/20);  total (16/20)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAGGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAATGCATTATATAAAATTTATTGAGTC  >  CP000730/76833‑77101
                                                                                                                                          |                                                                                                                                    
ttCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGAAAGTTAAACGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGt                                                                                                                                   <  1:518333/140‑1 (MQ=255)
   aGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTaa                                                                                                                                <  1:718578/140‑1 (MQ=255)
    gATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAAt                                                                                                                               >  1:693019/1‑140 (MQ=255)
     ctCTAGGGATTTTCGCCATGCTACGAAATTCGCATTATGCTAGCAAGTGAAGCGAACACTGCGATGATAAATTAGTGGTTAGCTACATTTTTTTTACTTTGCAACAGAACCTATAATAAACATGGGCTTAAAAGGTTAAt                                                                                                                               <  2:192672/139‑1 (MQ=14)
      tcaaCGGAGTTTAGACATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACCGACATGATAAATAAGTGGTTAGCTATATTTTTTTACTTTGCAAAAAAAAAAAAAAAAAACATGGGCTTAAAAGGTTAAttt                                                                                                                             <  2:504047/137‑1 (MQ=14)
         aCGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTtata                                                                                                                          <  1:427922/140‑1 (MQ=255)
            gATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAg                                                                                                                       >  2:546235/1‑140 (MQ=255)
                    ccacgccacgAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAAAAAAAAAAAAAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTg                                                                                                               <  2:467634/140‑1 (MQ=17)
                                aTTAGCATCATTCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGAtt                                                                                                   <  1:753568/140‑1 (MQ=255)
                                   aGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGCACCTATAAAATACATGGTGGTATAAGGTTTATTTATATAGAGTTAGTGCAAACTTTGATTTgg                                                                                                >  1:669961/1‑140 (MQ=17)
                                   aGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTgg                                                                                                <  2:693019/140‑1 (MQ=255)
                                   aGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTgg                                                                                                >  1:482603/1‑140 (MQ=255)
                                            cTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAAt                                                                                       <  1:230767/140‑1 (MQ=255)
                                               gCAAGTTAAGCGAACACTGAGATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGGAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCTAACATTGATTCGGGTCAAAAATTAg                                                                                    >  1:460922/1‑140 (MQ=25)
                                               gCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAg                                                                                    >  1:680801/1‑140 (MQ=255)
                                                     tAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTAt                                                                              <  2:39894/140‑1 (MQ=255)
                                                      aaGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTAtt                                                                             <  1:51848/140‑1 (MQ=255)
                                                       aGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTa                                                                            <  1:219066/140‑1 (MQ=255)
                                                       aGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTa                                                                            <  1:6040/140‑1 (MQ=255)
                                                             cacTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTaaaaaaa                                                                      >  2:331986/1‑140 (MQ=255)
                                                                       aTAAAATACTGGTAAGCTATATTTTTTTACTTTGCCACTGATCATATGAAAAACATGGCATTAAAAGCTTAATTGCTATAGTATTACTGCATACATTGATTTGGGTCGAACAATATGGGCATTAAAAAAATgaatgtata                                                            >  2:37230/1‑139 (MQ=255)
                                                                        tAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAAGgaatgaataa                                                           >  2:707420/1‑138 (MQ=255)
                                                                                       cTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCt                                            >  1:73988/1‑140 (MQ=255)
                                                                                       cTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCt                                            >  2:583209/1‑140 (MQ=255)
                                                                                          tatTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTTATTTGGGTCAAAAATTAGGGGTATTAAAAAAATTAATGAATCATAATTTTATCAAGCTGAt                                         <  2:96338/140‑1 (MQ=255)
                                                                                                  ttACTTTGCAACAGAACCTATAAAAAACATGGTCTTAAAAGGTTAATTTATATTGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGagag                                  <  1:613770/140‑1 (MQ=255)
                                                                                                  ttACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGagag                                  >  1:612014/1‑140 (MQ=255)
                                                                                                  ttACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGagag                                  <  1:502409/140‑1 (MQ=255)
                                                                                                   taacttGCAAAAAAACCTAAAAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCGAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGagag                                  <  1:690070/136‑1 (MQ=255)
                                                                                                       ttGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTaa                             <  1:81770/140‑1 (MQ=255)
                                                                                                       ttGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGAATGGAGAGGTTaa                             >  1:209478/1‑140 (MQ=255)
                                                                                                             cAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAATGCa                       >  1:55419/1‑140 (MQ=255)
                                                                                                                    tataAAAAACATGGGTTTAACAGGTTAATTTATATTGTCTTCCTCCTTTTACACATTTGGGTCAAAAATTAGGTGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAATGCATtatata                <  1:171544/140‑1 (MQ=255)
                                                                                                                        aaaaaaCATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGAATTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAATGCATTATATaaaa             >  1:401467/1‑140 (MQ=255)
                                                                                                                        aaaaaaCATGGGCTTAAAAGGTTAATATATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGACAGGTTAAAATGCATTATATaaaa             >  2:348437/1‑140 (MQ=255)
                                                                                                                                   gCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTTAAAAATTAGGGGTATTAAAAAAATGAATTAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAATGCATTATATAAAATTTATTGAGTc  <  2:259502/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                    
TTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAGGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAATGCATTATATAAAATTTATTGAGTC  >  CP000730/76833‑77101

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: