Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,241,539 T→C 60.8% intergenic (‑182/+284) murZ ← / ← fbaA UDP‑N‑acetylglucosamine 1‑carboxyvinyltransferase/fructose‑bisphosphate aldolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,241,5390TC60.8% 32.7 / 66.9 64intergenic (‑182/+284)murZ/fbaAUDP‑N‑acetylglucosamine 1‑carboxyvinyltransferase/fructose‑bisphosphate aldolase
Reads supporting (aligned to +/- strand):  ref base T (14/11);  new base C (18/21);  total (32/32)
Fisher's exact test for biased strand distribution p-value = 6.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.83e-02

TAGCATTATAAATTAATTTTACTTTATTTGTAAAATAGTTTATTTTAATCGTTAAAATCTTGTCTTTCTATTTTCGATTGATTAATAACAAATCATTCACCTTTACTAATATATGCTGTTCTTCCCCACTTGACCATGGTCTAAAGTCTGATTATTCAACTAAATATTGACGTTGTTTTGACAATTATTTAGTTTTAATTCTACGGTTTTCACGTAAACTTTTTTCATAAATTGCTCATTTTCCTTATAATTATATCACAATCAATTTTG  >  CP000730/2241403‑2241672
                                                                                                                                        |                                                                                                                                     
taGCATTATAAATTAATTTTACTTTATTTGTAAAATAGTTTATTTTAATCGTTAAAATCTTGTCTTTCTATTTTGGATTGATTAATAACAAATCATTCACCTTTACTAATATATGATGTTCTTCCCCACTTATACATGGt                                                                                                                                    >  2:25688/1‑140 (MQ=255)
  gCATTATAAATTAATTTTACTTTATTTGTAAAATAGTTTATTTTAATCGTTAAAATCTTGTCTTTCTATTTTCGATTGATTAATAACAAATCATTCACCTTTACTAATATATGCTGTTCTTCCCCACTTGACCACGGTCt                                                                                                                                  <  2:721822/140‑1 (MQ=255)
  gCATTATAAATTAATTTTACTTTATTTGTAAAATAGTTTATTTTAATCGTTAAAATCTTGTCTTTCTATTTTCGATTGATTAATAACAAATCATTCACCTTTACTAATATATGCTGTTCTTCCCCACTTGACCACGGTCt                                                                                                                                  <  2:561852/140‑1 (MQ=255)
  gCATTATAAATTAATTTTACTTTATTTGTAAAATAGTTTATTTTAATCGTTAAAATCTTGTCTTTCTATTTTCGATTGATTAATAACAAATCATTCACCTTTACTAACATATGCTGTTCTTCCCCACTTGACCACGGTCt                                                                                                                                  >  2:423787/1‑140 (MQ=255)
  gCATTAGAAATTAATTTTACTTTATTTGTAAAATAGTTTATTTTAATCGTTAAATTCTTGTCTTTCTATTTTCGATTGATTAATAACAAATCATTCACCTTTGCTAATATATGCTGTTCTTCCCCTCTTGACCACTGTCt                                                                                                                                  <  2:50178/140‑1 (MQ=255)
      tatacaagaactttACTTTATGTTTAAAATAGGTTATTGTAATCGTTAAAATCGTGTCTTTCTATTTTCGAGTGAGTAATAACAAATCATTCACCTTTATTAATATATGCTTTTCTTCCCCACTTGACCACGGTCTAAAg                                                                                                                              <  2:684372/129‑1 (MQ=255)
        taatttaattTTATTTTATTTGTAAAATAGTTTATTTTAATCGTTAAAATCTTGTCTTTCTATTTTCGATTGATTAATAACAAATCATTCACCTTTACTAATATATGCTGTTCTTCCCCACTTGACCATGGTCTAAAGTc                                                                                                                            <  1:102704/140‑1 (MQ=255)
          atttaattTTACTTTATTTGTAAAATAGTTTATTTTAATCGTTAAAATCTTCTCTTTCTATTTTCGATTGATTAATAACAAATCATTCACCTTTACTAATATATGCTGTTCTTCCCCACTTGACCACGGTCTAAAGTCTg                                                                                                                          <  2:652648/138‑1 (MQ=255)
              aattTTACTTTATTTGTAAAATAGTTTATTTTAATCGTTAAAATCTTGTCTTTCTATTTTCGATTGATTAATAACAAATAATTCACCTTTACTAATATATGCTGTTCTTCCCCACTTGACCACGGTCTAAAGTCTGatta                                                                                                                      >  1:232000/1‑140 (MQ=255)
                    aCTTTATTTGTAAAATAGTTTATTTTAATCGTTAAAATCTTGTCTTTCTATTTTCGATTGATTAATAACAAATCATTCACCTTTACTAATATATGCTGTTCTTCCCCACTTGACCACGGTCTAAAGTCTGATTATTCAAc                                                                                                                >  1:597683/1‑140 (MQ=255)
                    aCTTTATTTGTAAAATAGTTTATTTTAATCGTTAAAATCTTGTCTTTCTATTTTCGATTGATTAATAACAAATCATTCACCTTTACTAATATATGCTGTTCTTCCCCACTTGACCACGGTCTAAAGTCTGATTATTCAAc                                                                                                                >  1:66401/1‑140 (MQ=255)
                     cTTTTTTTGTAAAATAGTTTGTTTTAATCGTTAAAATCTTGTCTTTCTATTTTCGATTGATTAATAACAAATCATTCACCTTTACTAATATATGCTGTTCTTCCCCACTTGACCACGGTCTAAAGTCTGATTATTCAACt                                                                                                               <  1:170793/140‑1 (MQ=255)
                         aTTTTTAAAATAGTTTATTTTAATCGTTAAAATCTTGTCTTTCTATTTTCGATTGATTAATAACAAATCATTCACCTTTACTAATATATTCTGTTCTTCACCACTTGACCACGGTCTAAAGTCTGATTATTCAACTTAat                                                                                                           <  2:11739/140‑1 (MQ=255)
                         aTTTGTAAAATAGTTTATTTTAATCGTTAAAATCTTGTCTTTCTATTTTCGATTGATTAATAACAAATCATTCACCTTTACTAATATATGCTGTTCTTCCCCACTTGACCACGGTCTAAAGTCTGATTATTCAACTAAat                                                                                                           <  1:182417/140‑1 (MQ=255)
                             gTAAAATAGTTTATTTTAATCGTTAAAATCTTGTCTTTCTATTTTCGATTGATTAATAACAAATCATTCACCTTTACTAATATATGCTGTTCTTCCCCACTTGACCATGGTCTAAAGTCTGATTATTCAACTAAATATTg                                                                                                       <  1:501421/140‑1 (MQ=255)
                                taaTAGTTTATTTTAATCGTTAAAATCTTGTCTTTCTATTTTCGATTGATTAATAACAAATCATTCACCTTTACTAATATATGCTGTTCGTCCCCACTTGACCACGGTCTAAAGTCTGATTATTCAACTAAATATTGACg                                                                                                    <  1:592413/139‑1 (MQ=255)
                                aaaTAGTTTATTTTAATCTTTAAAATCTTGTCTTTCTATTTTCGATTGATTAATAACAAATCATTCACCTTTACTAATATATGCTGTTCTTCCCCACTTGACCATGGTCTAAAGTCTGATTATTCAACTAAATATTGACg                                                                                                    <  2:330092/140‑1 (MQ=255)
                                 aaTAGTTTATTTTAATCGTTAAAATCTTGTCTTTCTATTTTCCATTGATTAATAACAAATCATTCACCTTTACTAATATATGCTGTTCTTCCCCACTTGACCACGGTCTAAAGTCTGATTATTCAACTAAATATTGACgt                                                                                                   <  2:749820/140‑1 (MQ=255)
                                    aGTTTATTTTAATCGTTAAAATCTTGTCTTTCTATTTTCGATTGATTAATAACAAATCATTCACCTTTACTAATATATGCTGTTCTGCCCCACTTGACCACGGTCTAAAGTCTGATTATTCAACTAAATATTGACgttgt                                                                                                <  1:162467/140‑1 (MQ=255)
                                     gTTTATTTTAATCGTTAAAATCTTTTTAATCTATTTTCGATTGATTAATAACAAATCATTCACCTTTACTAATATATGCTGTTATTCCCCACTTGACCACGGTCTAAAGTCTGATTATTCAACTATAAATTTACgttgtt                                                                                               >  2:349493/1‑140 (MQ=255)
                                       ttATTTTAATCGTTAAAATCTTGTCTTTCTATTTTCGATTGATTAATAACAAATCATTCACCTTTACTAATATATGCTGTTCTTCCCCACTTGACCACGGTCTAAAGTCTGATTATTCAACTAAATATTGACGTTGtttt                                                                                             >  2:39065/1‑140 (MQ=255)
                                        tATTTTAATCGTTAAAATCTTGTCTTTCTATTTTCGATTGATTAATAACAAATCATTCACCTTTACTAATATATGCTGTTCTTCCCCACTTGACCACGGTCTAAAGTCTGATTATTCAACTAAATATTGACGTTGTTTTg                                                                                            <  2:626123/140‑1 (MQ=255)
                                            ttAATCGTTAAAATCTTGTCTTTCTATTTTCGATTGATTAATAACAAATCATTCACCTGTACTAATATATGCTGTTCTTCCCCACTTGACCATGGTCTAAAGTCTGATTATTCAGCTAAATATTGACGTTGTTTTGACaa                                                                                        >  1:467001/1‑140 (MQ=255)
                                               aTCGTTAAAATCTTGTCTTTCTATTTTCGATTGATTAATAACAAATCATTCACCTTTACTAATATATGCTGTTCTTCCCCACTTGACCACGGTCTAAAGTCTGATTATTCAACTAAATATTGACGTTGTTTTGACAatta                                                                                     >  2:446411/1‑140 (MQ=255)
                                               aTCGTTAAAATCTTGTCTTTCTATTTTCGATTGATTAATAACAAATCATTCACCTTTACTAATATATGCTGTTCTTCCCCACTTAACCACGGTCTAAAGTCTGATTATTCAACTAAATATTGACGTTGTTTTGACAatta                                                                                     >  1:68669/1‑140 (MQ=255)
                                                   ttAAAATCTTGTCTTTCTATTTTCGATTGATTAATAACAAATCATTCACCTTTACTAATATATGCTGTTCTTCCCCACTTGACCATGGTCTAAAGTCTGATTATTCAACTAAATATTGACGTTGTTTTGACAATTATTTa                                                                                 <  2:243133/140‑1 (MQ=255)
                                                       aagcTTATCTTTCTATTTTCGATTGATTAATAACAAATCATTCACCTTTACTAATATATGCTGTTCTTCCCCACTTGACCACGGTCTAAAGTCTGATTATTCAACTAAGTCTGGGGGTTTTTGTGACAATTATTTAGttt                                                                             <  2:734604/137‑1 (MQ=255)
                                                           ttGTCTTTCTATTTTCGATTGATTAATAACAAATCATTCACCTTTACTAATATATGCTGTTCTTCCCCACTTGACCATGGTCTAAAGTCTGATTATTCAACTAAATATTGACGTTGTTTTGACAATTATTTAGTTTTAAt                                                                         >  1:640560/1‑140 (MQ=255)
                                                           ttGTCTTTCTATTTTCGATTGATTAATAACAAATCATTCACCTTTACTAATATATGCTGTTCTTCCCCACTTGACCATGGTCTAAAGTCTGATTATTCAACTAAATATTGACGTTGTTTTGACAATTATTTAGTTTTAAt                                                                         >  1:163729/1‑140 (MQ=255)
                                                             gTCTTTCTATTTTCGATTGATTAATAACAAATCATTCCCCTTTACTAATACATGCTGTTCTTCCCCACTTGACCATGGTCTAAAGTCTGATTATTCAACTAAATATTGACGTGGTGTTGAAAATTATTTAGTTTTAATTc                                                                       <  2:502925/140‑1 (MQ=255)
                                                              tCTTTCTATTTTCGATTGATTAATAACAAATCATTCACCTTTACTATTATATGCTGTTCTTCCCCAATTGACCACGGTCTAAATTCTGATTATTCAACTAAATATTGACGTTGTTTTGACAATTATTTAGTTTTAATTCt                                                                      <  1:405066/140‑1 (MQ=255)
                                                              tCTTTCTATTTTCGATTGATTAATAACAAATCATTCACCTTTACTAATATATGCTGTTCTTCCCCACTTGACCACGGTCTAAAGTCTGATTATTCAACTAAATATTGACGTTGTTTTGACAATTATTTAGTTTTAATTCt                                                                      <  1:84838/140‑1 (MQ=255)
                                                                 ttCTATTTGCGATTGATTAATAACAAATCATTCACCTTTACTAATATATGCTGTTCTTCCCCACTTGACCACGGTCTAAAGTCTGATTATTCAACTAAATATTGACGTTGTTTTGACAATTATTTAGTTTTAATTCTACg                                                                   <  2:528271/140‑1 (MQ=255)
                                                                           gattgattAATAACAAATCATTCACCTTTACTAATATATGCTGTTCTTCCCCACTTGACCACGGTCTAAAGTCTGATTATTCAACTAAATATTGACGTTGTTTTGACAATTATTTAGTTTTAATTCTACGGTTTTCACGt                                                         <  1:39065/140‑1 (MQ=255)
                                                                              tgattAATAACAAATCATTCACCTTTACTAATATATGCTGTTCTTCCCCACTTGACCACGGTCTAAAGTCTGATTATTCAACTAAATATTGACGTTGTTTTGACAATTATTTAGTTTTAATTCTACGGTTTTTACGTaaa                                                      >  1:724710/1‑140 (MQ=255)
                                                                                     taaCAAATCATTCATCTTTACTAATATATGCTGTTCTTCCCCACTTGACCATGGTCTAAAGTCTGATTATTCAACTAAATATTGACGTTGTTTTGACAATTATTTAGTTTTAATTCTACGGTTTTCACGTAAACcttttt                                               <  1:25688/140‑1 (MQ=255)
                                                                                       aCAAACCATTCACCTTTACTAATATATGCTGTTCTCCCCCACTTGACCATGGTCTAAAGTCTGATTATTCAACTAAATATTGACGTTGTTTTGACAATTATTTAGTTTTAATTCTACGGTTTTCACGTAAACTTTTTTCa                                             <  2:523851/140‑1 (MQ=255)
                                                                                         aaaTCATTCACCTTTACTAATATATGCTGTTCTTCCCCACTTGACCACGGTCTAAAGTCTGATTATTCAACTAAATATTGACGTTGTTTTGACAATTATTTAGTTTTAATTCTACGGTTTTCACGTAAACTTTTTTCATa                                           >  2:702207/1‑140 (MQ=255)
                                                                                          aaTCATTCACCTTTACTAATATATGCTGTTCTTCCCCACTTGACCACGGTCTAAAGTCTGATTATTCAACTAAATATTGACGTTGTTTTGACAATTATTTAGTTTTAATTCTACGGTTTTCACGTAAACTTTTTTCATaa                                          >  2:729591/1‑140 (MQ=255)
                                                                                            tCATTCACCGTTACTAATATATGCTGTTCTTCCCCACTTGACCATGGTCTAAAGTCTGATTATTCAACTAAATATTGACGTGGTTTTGACAATTATTTAGTTTTAATTCTACGGTTTTCACGTAAACTTTTTTCATaact                                        >  1:557953/1‑138 (MQ=255)
                                                                                             cATTCACCTTTACTAATATATGCTGTTCTTCCCCACTTGACCACGGTCTAAAGTCTGATTATTCAACTAAATATTGACGTTGTTTTGACAATTATTTAATTTTAATTCTACGGTTTTCACGTAAACTTTTTTCATAAAtt                                       >  1:98901/1‑140 (MQ=255)
                                                                                               ttCACCTTTACTAATATATGCTGTTCTTCCCCACTTGACCATGGTCTAAAGTCTGCTTATTCAACTAAATATTGACGTTGTTTTGACAATTATTTAGTTTTAATTCTACGGTTTTCACGTAAACTTTTTTCATAAATTGc                                     >  1:117579/1‑140 (MQ=255)
                                                                                                tCACCTTTACTAATATACGCTGTTCTTCCCCACTTGACCACGGTCTAAAGTCTGAATATTCAACTAAATATGGACGTTGTCTTGACAATTATTTAGCTTTGATTCTACGGATTTCACGTAAATTTTTTTCTTAATTTGCt                                    >  2:67032/1‑140 (MQ=255)
                                                                                                    cTTTACTAATATATGCTGTTCTTCCCAACTTGACCATGGTCTAAAGTCTGATTATTCAACTAAATATTGACGTTGTTTTGACAATTATTGAGTTTTAATTCTACGGTTTTCACGTAAACTTTTTTCATAAATTGCTCAtt                                <  1:632485/140‑1 (MQ=255)
                                                                                                     tttACTAATATATGCTGTTCTTCCCCACTTTACCACGGTCTAAAGTCTGATTATTCAACTAAAGATTGACGTTGTGTTGACTATTATTTAGTTGTAATTCTACGGTTTTCACGTAAACTTTTTTCATAAATTGCTCAttt                               <  2:66401/140‑1 (MQ=255)
                                                                                                     tttACTAATATATGCTGTTCTTCCCCACTTGACCACGGTCTAAAGTCTGATTATTCAACTAAATATTGACGTTGTTTTGACAATTATTTAGTTTTAATTCTACGGTTTTCACGTAAACTTTTTTCATAAATTGCTCAttt                               <  2:597683/140‑1 (MQ=255)
                                                                                                      gtgcTAATATTTGCTGTTCTTCCCCTCTTGGCCATGGTCTAAAGTCTGATTATTCAACTAAATATTGACGTTGTTTTGACAATTATTTAGTTTTAATTCTACGGTTTTCACGTAAACTGTTTTCATAAATTGTTCAtttt                              <  2:219044/137‑1 (MQ=255)
                                                                                                       tACTAATATATGCTGTTCTTCCCCACTTGACCACGGTCTAAAGTCTGATTATTCAACTAAATATTGACGTTGTTTTGACAATTATTTAGTTTTAATTCTACGGTTTTCACGTAAACTTTTTTCATAAATTGCTCATTTTc                             <  1:288308/140‑1 (MQ=255)
                                                                                                         ttAATATATGCTTTTCTTCCCCACTTGACCATGGTCTAAAGTCTGATTATTCAACTAAATATTGACGTTGGTTTGACAATTATTTATTTTTAATTTTACTGTTCTCACGTAAACTTTTTTCATAAATTTCTCATTTTCCt                           <  2:249041/139‑1 (MQ=255)
                                                                                                                   cTGTTCTTCCCCACTTGACCATGGTCTAAAGTCTGATTATTCAACTAAATATTGACGTTGTTTTGACAATTATTTAGTTTTACTTCTACGGTTTTCACGTAAACTTTTTTCATAAATTGCTCATTTTCCTTATAATtata                 >  1:288037/1‑140 (MQ=255)
                                                                                                                   cTGTTCTTCCCCACTTGACCATGGTCTAAAGTCTGATTATTCAACTAAATATTGACGTTGTTTTGACAATTATTTAGTTTTAATTCTACGGTTTTCACGTAAACTTTTTTCATAAATTGCTCATTTTCCTTATAATtata                 >  2:31861/1‑140 (MQ=255)
                                                                                                                   cTGTTCTTCCCCACTTGACCATGGTCTAAAGTCTGATTATTCAACTAAATATTGACGTTGTTTTGACAATTATTTAGTTTTAATTCTACGGTTTTCACGTAAACTTTTTTCATAAATTGCTCATATTCCTTATAATtata                 >  1:157402/1‑140 (MQ=255)
                                                                                                                     gTTCTTCCCCACTTGACCATGGTCTAAAGTCTGATTATTCAACTAAATATTGACGTTGTTTTGACAATTATTTAGTTTTAATTTTACGGTTTTCACGTAAACTTTTTTCATTAATTGCTCATTTTCCTTATAATTATATc               >  2:227579/1‑140 (MQ=255)
                                                                                                                     gTTCTTCCCCACTTGACCATGGTCTAAAGTCTGATTATTCAACTAAATATTGACGTTGTTTTGACAATTATTTAGTTTTAATTCTACGGTTTTCACGTAAACTTTTTTCATAAATTGTCTATTTTCCTTATAATTATATc               >  1:215079/1‑140 (MQ=255)
                                                                                                                     gTTCTTCCCCACTTGACCATGGTCTAAAGTCTGATTATTCAACTAAATATTGACGTTGTTTTGACAATTATTTAGTTTTAATTCTACGGTTTTCACGTAAACTTTTTTCATAAATTGCTCATTTTCCTTATAATTATATc               >  2:198025/1‑140 (MQ=255)
                                                                                                                        cttcCCCACTTGACCACGGTCTAAAGTCTGATTATTCAACTAAATATTGACGTTGTTTTGACAATTATTTAGTTTTAATTCTACGGTTTTCACGTAAACTTTTTTCATAAATTGCTCATTTTCCTTATAATTATATcaca            <  1:446411/140‑1 (MQ=255)
                                                                                                                        cttcCCCACTTGACCACGGTCTAAAGTCTGATTATTCAACTAAATATTGACGTTGTTTTGACAATTATTTAGTTTTAATTATTCGGTTTTCACGTAAACTTTTTTCATAAATTGCTCATTTTCCTTATAATTATATtaca            >  2:175465/1‑140 (MQ=255)
                                                                                                                         ttcCCCACTTGACCATGGTCTAAAGTCTGATTATTCAACTAAATATTGACGTTGTTTTGACAATTATTTAGTTTTAATTCTACGGTTTTCACGTAAACTTTTTTCATAAATTGCTCATTTTCCTTATAATTATATCAcaa           <  1:574234/140‑1 (MQ=255)
                                                                                                                           ccccACTTGACCATGGTCTAAAGTCTGATTATTCAACTAAATATTGACGTTGTTTTGACAATTATTTAGTTTTAATTCTACGGTTTTCACGTAAACTTTTTTCATAAATTGCTCATTTTCCTTATAATTATATCAcaatc         >  2:484183/1‑140 (MQ=255)
                                                                                                                           ccccACTTGACCACGGTCTAAAGTCTGATTATTCAACTAAATATTGACGTTGTTTTGACAATTATTTAGTTTTAATTCTACGGTTTTCAAGTAAACTTTTTTCATAAATTGCTCATTTTCCTTATAATTATATCAcaatc         >  1:397255/1‑140 (MQ=255)
                                                                                                                             ccACTTGACCACGGTCTAAAGTCTGATTATTCAACTAAATATTGCCGTTGTTTTGACAATTATTTAGTTTTAATTCTACGGTTTTCACGTAAACTTTTTTCATAAATTGCTCATTTTCCTTATAATTATATCAcaatcaa       >  1:221086/1‑140 (MQ=255)
                                                                                                                             ccACTTGACCACGGTCTAAAGTCTGATTATTCAACTAAATATTGACGATGTTTTGACAATTATTTAGTTTTAATTCTACGGTTTTGACGTAAACTTTTTTCATAAATTGCTCATTTTCCTTATAATTATATCAcaatcaa       >  2:41997/1‑140 (MQ=255)
                                                                                                                                cTTGACCACGGTCTAAAGTCTGATTATTCAACTAAATATTGACGTTGTTTTGACAATTATTTAGTTTTAATTCTACGGTTTTCACGTAAACTTTTTTCATAAATTGCTCATTTTCCTTATAATTATGTCACAATCAAttt    >  1:543019/1‑140 (MQ=255)
                                                                                                                                  tGACCATGGTCTAAAGTCTGATTATTCAACTAAATATTGACGTTGTTTTGACAATTATTTAGTTTTAATTCTACGGTTTTCACGTAAACTTTTTTCATAAATTGCTCATTTTCCTTATAATTATATCACAATCAATTTTg  >  2:169770/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                     
TAGCATTATAAATTAATTTTACTTTATTTGTAAAATAGTTTATTTTAATCGTTAAAATCTTGTCTTTCTATTTTCGATTGATTAATAACAAATCATTCACCTTTACTAATATATGCTGTTCTTCCCCACTTGACCATGGTCTAAAGTCTGATTATTCAACTAAATATTGACGTTGTTTTGACAATTATTTAGTTTTAATTCTACGGTTTTCACGTAAACTTTTTTCATAAATTGCTCATTTTCCTTATAATTATATCACAATCAATTTTG  >  CP000730/2241403‑2241672

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑