Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,409,590 A→G 5.9% Q127R (CAA→CGA)  ureG → urease accessory protein UreG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,409,5900AG 5.9% 218.1 / 3.1 80Q127R (CAA→CGA) ureGurease accessory protein UreG
Reads supporting (aligned to +/- strand):  ref base A (31/42);  new base G (4/2);  total (36/44)
Fisher's exact test for biased strand distribution p-value = 3.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01

AACTTTGCAGCAATAGACGAATTATTAGAACGTCATGACGATATAGAACTAATTTTCATAGAATCTGGTGGCGATAACTTAGCAGCAACATTTAGTCCAGAACTTGTTGACTTTTCAATATATATTATCGATGTTGCTCAAGGTGAAAAGATTCCACGTAAAGGTGGTCAAGGTATGATTAAGTCAGATTTCTTTGTAATTAACAAAACTGATTTAGCTCCCTATGTAGGTGCATCATTAGAACAAATGGCTGAAGATACTAAAGTATTTCGTGGTA  >  CP000730/2409451‑2409727
                                                                                                                                           |                                                                                                                                         
gaCTTTTGAGCAATAGACGAATTATTAGAACGTCATGGCAATGTAGCACTAGTGTTCATAGTATCTGGTTGCGATAACTTAGCAGCTCCATTTAGTCCAGAACTTGTGGAGTTTTCAATGTGTATTATCGAGGTTGCTCa                                                                                                                                           <  2:177362/139‑1 (MQ=255)
aaCTTTGCAGCAATAGACGGATTATTAGAACGTCATGACGATATAGAACTAATTTTCATAGAATCTGGTGGCGATAACTTAGCAGCAACATTTAGTCCAGAACTTGTTGACTTTTCAATATATATTATCGATGTTGCTCa                                                                                                                                           <  2:726355/140‑1 (MQ=255)
aaCTTGTCAGCAATAGACGAATTATTAGAACGTCATGACGATATAGAACTAATTTTCATAGAATCTGGTGGCGATAACTTAGCAGCAACATTTAGTCCAGAACTTGTTGACTTTTCAATATCTATTATCGATGTTGCTCa                                                                                                                                           <  2:576590/140‑1 (MQ=255)
   ttttCAGCAATAGACGAATTATTAGAACGTCATGACGATATAGAACTAATTTTCATAGAATCTGGTGGCGATAACTTAGCAGCAACATTTAGTCCAGAACTTGTTGACTTTTCAATATATATTATCGATGTTGCTCAAgg                                                                                                                                        <  1:281718/140‑1 (MQ=255)
        agcaATAGACGAATTATTAGAACGTCATGACGATATAGAACTAATTTTCTTAGAATCTGGTTGCGATAACTTATTAGCAATATTTAGTCCAGAACTTGTTGACTTTTCAATATATATTATCGATGTTGCTCAAGGTGaaa                                                                                                                                   <  1:8850/140‑1 (MQ=255)
         gcaATAGACGAATTATTAGATCGTCATGACGATATAGAACTAATTTTCATAGAATCTGGTGGCGATAACTTAGCAGCAACATTTAGTCCAGAACTTGTTGACTTTTCAATATATATTATCGATGTTGCTCAAGGTGaaaa                                                                                                                                  <  1:742887/140‑1 (MQ=255)
             tAGACGAATTATTAGAACGTCATGACGATATAGAACTAATTTTCATAGAATCTGGTGGCGATAACTTAGCAGCAACATTTAGTCCAGAACTTGTTGACTTTTCAATATATATTATCGATGTTGCTCAAGGTGAAAAGAtt                                                                                                                              <  1:695299/140‑1 (MQ=255)
                  gAATTATTAGAACGTCATGACGATATAGAACTAATTTTACTAGAACATGGAGGCAATAACTGATCATCAACATTTACTGGAATTACGGCATACGTGACAATATATATGATCGATGATTCTCAAGGTGAAAAGATTCAACg                                                                                                                         >  2:345860/1‑140 (MQ=255)
                       attaGAATGTTATGACGGTATCGAACTGATTTTTATAGATTCTGGTGGTGATAACTTAGCAGCAACATTTAGTTCAGAATTTGTTGACTTTTCAATATATATTATCGATGTTGCTCAAGGTGAAAAGATTCCACGTAAAg                                                                                                                    <  1:343461/140‑1 (MQ=255)
                       attaGAACGTCATGACGATATAGAACTAATTTTCATAGAATCTGGTGGCGATAACTTAGCAGCAACATTTAGTCCAGAACTTGTTGACTTTTCAATATATATTATCGATGTTGCTCCAGGTGAAAAGATTCCACGTAAAg                                                                                                                    >  2:427908/1‑140 (MQ=255)
                       attaGAACGTCATGACGATATAGAACTAATTTTCATAGAATCTGGTGGCGATAACTTAGCAGCAACATTTAGTCCAGAACTTGTTGACTTTTCAATATATATTATCGATGTTGCTCAAGGTGAAAAGATTCCACGTAAAg                                                                                                                    <  1:296604/140‑1 (MQ=255)
                        ataGAACGTCATGACGATATAGAACTAATTTTCATAGAATCTGGTGGCGATAACTTAGCAGCAACATTTAGTCCAGAACTTGTTGACTTTTCAATATATATTATCGATGTTGCCCAAGGTGAAAAGATTCCACGTAAAgg                                                                                                                   >  2:542478/2‑140 (MQ=255)
                         tagacgcgcATGACGCTATAGAACTAATTTTCATAGAATCTGGTGGCGATAACTTAGCAGCAACATTTAGTCAAGAACTTGTTGACTGTTCAATATATATTATCGATGTTGCTCAAGGTGAAAAGATTCCACGTAAAggt                                                                                                                  <  2:568792/132‑1 (MQ=255)
                         taGAACGTCATGACGTTATAGGTCTAATTTTCATAGAATCTGGTGGCGATTTCTTAGCAGCATCATTTAGTCCAGAACTTGTTGACTTTTCAATATATATTATCGATGTTGCTCAAGGTGAAAAGATTCCACGTAAAggt                                                                                                                  <  1:121676/140‑1 (MQ=255)
                         taGAACGTCATGACGATATAGAACTAATTTTCATAGAATCTGGTGGCGATAACTTAGCAGCAACATTTAGTCCAGAACTTGTTGACTTTTCAATATATATTATCGATGTTGCTCAAGGTGAAAAGATTCCACGTAAAggt                                                                                                                  >  1:749702/1‑140 (MQ=255)
                         taGAACGTCATGACGATATAGAACTAATTTTCATAGAATCTGGTGGCGATAACTTAGCAGCAACATTTAGTCCAGAACTTGTTGACTTTTCAATATATATTATCGATGTTGCTCAAGGTGAAAAGATTCCACGTAAAggt                                                                                                                  <  1:684956/140‑1 (MQ=255)
                         taGAACGTCATGACGATATAGAACTAATTTTCATAGAATCTGGTGGCGATAACTTAGCAGCAACATTTAGTCCAGAACTTGTTGACTTTTCAATATATATTATCGATGTTGCTCAAGGTGAAAAGATTCCACGTAAAggt                                                                                                                  <  2:147709/140‑1 (MQ=255)
                           gAACGTCATGACGATATAGAACTCCCTAAAATATTATCTGGTGGCGATAACGTAGCAGCAACATTTAGTCCAGAACTTGTTGACTTTGCAATAAATATTATCGATGTTGGTCGTGGTGAAAAGATTCCAATTAAAtgtgg                                                                                                                >  1:367561/1‑140 (MQ=255)
                           gAACGTCATGACGATATAGAACTAATTTTCATAGAATCTGGTGGCGATAACTTAGCAGCAACATTTAGTCCAGAACTTGTTGACTTTTCAATATATATTATCGATGTTGCTCAAGGTGAAAAGATTCCACGTAAAggtgg                                                                                                                <  1:108422/140‑1 (MQ=255)
                                 cATGACGATATAGAACTAATTTTCATAGAATCTGGTGGCGATAACTTAGCAGCAACATTTAGTCCAGAACTTGTTGACTTTTCAATATATATTATCGATGTTGCTCAAGGTGAAAAGATTCCACGTAAAGGTGGTCAAgg                                                                                                          <  1:170161/140‑1 (MQ=255)
                                   tGACGATATAGAACTAATTTTCATAGAATCTGGTGGCGATAACTTAGCAGCAACATTTAGTTCAGTACTTGTTGACTTTTCAATATATATTATCGATGTTGCTCAAGGTGAAAAGATTCCACGTAAAGGTGGTCAAGGTa                                                                                                        <  1:78975/140‑1 (MQ=255)
                                   tGACGATATAGAACTAATTTTCATAGAATCTGGTGGCGATAACTTAGCAGCAACATTTAGTCCAGAACTTGTTGACTTTTCAATATATATTATCGATGTTGCTCAAGGTGAAAAGATTCCACGTAAAGGTGGTCAAGGTa                                                                                                        <  1:468318/140‑1 (MQ=255)
                                     aCGATATAGAACTAATTTTCCTAGAATCTGGTGGCGATAACTTACCAGCAACATTTAGTCCAGAACTTGTTGACTTTTCAATATATATTATCGATGTTGCTCAAGGAGAAAAGACTCCACGTAAAGGTGGTCAAGGTATg                                                                                                      >  1:462341/1‑140 (MQ=255)
                                       gATATAGAACTAATTTTCATAGAATCTGGTGGCGATAACTTAGCAGCAACATTTAGTCCAGAACTTGTTGACTTTTCAATATATATTATCGATGTTGCTCAAGGTGAAAAGATTCCACGTAAAGGTGGTCAAGGTATGAt                                                                                                    <  1:733548/140‑1 (MQ=255)
                                       gATATAGAACTAATTTTCATAGAATCTGGTGGCGATAACTTAGCAGCAACATTTAGTCCAGAACTTGTTGACTTTTCAATATATATTATCGATGTTGCTCAAGGTGAAAAGATTCCACGTAAAGGTGGTCAAGGTATGAt                                                                                                    >  1:291765/1‑140 (MQ=255)
                                       gATATAGAACGAATTTACATAGAATCTGGTGGCGATAACTTAGCAGCAACATTTAGTCAAGAACTTGTTGACTTTTCAATATATATTATCGATGTTGCTCGAGGTGAAAAGATTCCACGTAAAAGGGGTCAAGGTCTGAt                                                                                                    >  2:206239/1‑140 (MQ=255)
                                         tataGAACTAATTTTCATAGAATCTGGTGGCGATAACTTAGCAGCAACATTTAGTCCAGAACTTGTTGACTTTTCAATATATATTATCGATGTTGCTCAAGGTGAAAAGATTCCACGTAAAGGTGGTCAAGGTATGATTa                                                                                                  >  1:608813/1‑140 (MQ=255)
                                           taGTACTAATTTTCATAGAATGTGGTGGCGATAACTTAGCAGCAACATTTAGTCCAGAACTTGTTGACTTTTCAATATATATTATCGGTGTTGCTCAAGGTGAAAAGATTCCACGTAAAGGTGGTCAAGGTATGATTAAg                                                                                                <  2:176204/140‑1 (MQ=255)
                                                  aaTTCTCATAGAATCTGGTGGCGATAACTTAGCAGCAACATTTAGTCCAGAACGTGTTGACTTTTCAATATATATTATCGATGTTGCTCAAGGTGAAAAGATTCCACGTAAAGGTGGTCAAGGTATGATTAAGTCAGAtt                                                                                         <  2:722293/140‑1 (MQ=255)
                                                   tttttCATAGAATCTGGTGGCGATAACTTAGCAGCAACATTTAGTCCAGAACTTGTTGACTTTTCAATATATATTATCGATGTTGCTCAAGGTGAAAAGATTCCACGTAAAGGTGGTCAAGGTATGATTAAGTCAGAttt                                                                                        <  2:358109/139‑1 (MQ=255)
                                                      ttCATAGAATCTGGTGGCGATAACTTAGCAGCAACATTTAGTCCAGAACTTGTTGACTTTTCAATATATATTATCGATGTTGCTCAAGGTGAAAAGATTCCACGTAAAGGTGGTCAAGGTATGATTAAGTCAGATTTCtt                                                                                     <  1:688900/140‑1 (MQ=255)
                                                      ttCATAGAATCTGGTGGCGATAACTTAGCAGCAACATTTAGTCCAGAACTTGTTGACTTTTCAATATATATTATCGATGTTGCTCAAGGTGAAAAGATTCCACGTAAAGGTGGTCAAGGTATGATTAAGTCAGATTTCtt                                                                                     >  2:606028/1‑140 (MQ=255)
                                                        cATAGAATCTGGCGGCGATAACTTAGCAGCAACAATTAGTCCAGAAGTTGTTGACTTTTCAATATACATTATCGACGTTGCTCAAGGTGAAAACATTCCACGTAAAGGTGGTCAAGGAATGATTAAGCAAGATTTCTTTg                                                                                   >  2:83721/1‑140 (MQ=255)
                                                          tAGAATCTGGTGGCGATAACTTAGCAGCAACATTTAGTCCAGAACTTGTTGACTTTTCAATATATATTATCGATGTTGCTCAAGGTGAAAAGATTCCACGTAAAGGTGGTCAAGGTATGATTAAGTCAGATTTCTTTGTa                                                                                 >  1:383556/1‑140 (MQ=255)
                                                           aGAATCTGGTGGTGAAAACTTAGCAGCAACATGTAGTCCAGAACTTGTTGACTTTTCAATATATATTATCGATGTTGCTCAAGGTGAAAAGATTCCACGTAAAGGTGGTCAAGGTATGATTAAGTCAGATTTCTTTGTaa                                                                                <  2:380008/140‑1 (MQ=255)
                                                           aGAATCTGGTGGCGATAACTTAGCAGAACAATTTAGTCCAGAACTTGTTGACTTTTCAATATATATTATCGATGTTGCTCAAGGTGAAAAGATTCCACGTAAAGGTGGTCAAGGTATGATTAAGTCAGATTTCTTTGTaa                                                                                >  2:128536/1‑140 (MQ=255)
                                                              atcttgaagcaaTAACTTAGCAGCAACATTTAGTCCAGAACTTGTTGACTTTTCAATATCTATTATCGATGTTGCTCAAGGTGAAAAGATTCCACGTAAAGGGGGTCAAGGTATGATTAAGTCAGATTTCTTTGTAATTa                                                                             >  2:290167/12‑140 (MQ=255)
                                                              aTCTGGTGGTGATACCTTAGCAGCAACATTTAGTCCAGAACTTGTTGATTTTTCAATATATATTATCGATGTTGCGCAAGGTGAAAAGATTCCACGTAAAGGTGGTCAAGGTATGATTAAGTCAGATTTCTTTGTAATTa                                                                             <  1:264875/140‑1 (MQ=255)
                                                              aTCTGGTGGCGATAACTTAGCAGCAACATTTAGTCCAGAACTTGTTGACTTTTCAATATATATTATCGATGTTGCTCAAGGTGAGCACATTCCAAGTAAAGGGATTCAAGGTATGATTAAGTCAGATTTCTTTGTAATTa                                                                             <  1:364682/140‑1 (MQ=255)
                                                              aTCTGGTGGCGATAACTTAGCAGCAACATTTAGTCCAGAACTTGTTGACTTTTCAATATATATTATCGATGTTGCTCAAGGTGAAAAGATTCCACGTAAAGGTGGTCAAGGTATGATTAAGTCAGATTTCTTTGTAATTa                                                                             <  2:169056/140‑1 (MQ=255)
                                                              aTCTGGTGGCGATAACTTAGCAGCAACATTTAGTCCAGAACTTGTTGACTTTTCAATATATATTATCGATGTTGCTCAAGGTGAAAAGATTCCACGTAAAGGTGGTCAAGGTATGATTAAGTCAGATTTCTTTGTAATTa                                                                             >  1:427192/1‑140 (MQ=255)
                                                                 tggtggCGATAACTTAGCAGCAACATTTAGTCCAGAACTTGTTGACTTTTCAATATATATTATCGATGTTGCTCAAGGTGAAAAGATTCCACGTAAAGGTGGTCAAGGTATGATTAAGTCAGATTTCTTTGTAATTAACa                                                                          <  1:247638/140‑1 (MQ=255)
                                                                 tggtggCGATAACTTAGCAGCAACATTTAGTCCAGAACTTGTTGACTTTTCAACATATATTATCGATGTTGCTCAAGGTGAAAAGATTCCACGTAAAGGTGTTCAAGGTATGATTAAGTCCGATTTCTTTGTAATTAACa                                                                          <  1:424227/140‑1 (MQ=255)
                                                                        gATAACTTAGCAGCAACATTTAGTCCAGAACTTGTTGACTTTTCAATATATATTATCGATGTTGCTCAAGGTGAAAAGATTCCACGTAAAGGTGGTCAAGGTATGATTAAGTCAGATTTCTTTGTAATTAACAAAACTGa                                                                   <  2:749702/140‑1 (MQ=255)
                                                                         ataagatcgacgaaaCAGTGAGTCCAGAACTTGTTGACTGTTCAATATATATTATCGATGTTGCTCAAGGTGAAAAGATTCCACGTAAAGGTGGTCAAGGTATGATTAAGTCAGATTTCTTTGTAATTAACAAAACTGAt                                                                  >  2:352352/14‑140 (MQ=255)
                                                                          taaactAGCAGCAACATTTAGTCCATAACTTGGTGACTTTTCAATATATATTATCGATGTTGCTCAAGGTGAAAATATTCCACGTAAAGGTGGTCAAGGTATGATTAACTCAGATACCCGTGTAATTAACAAAACTGAtt                                                                 <  2:349576/135‑1 (MQ=255)
                                                                          tAACATTGCAGCAACATTTAGTCCAGAACTCTTTGACTTTTCAATATATATTATCAATGTTGCTCAATGTGAAAAGATTCCACGGAAAGGAGGTCAAGGTATGATCAAGTCAGATTTCTTTGTAATTAACAAAACTGAtt                                                                 <  1:626829/140‑1 (MQ=255)
                                                                             cTTAGCAGCAACATTTAGTCCAGAACTTGTTGACTTTTCAATATATATTATCGATGTTGCTCGAGGTGAAAAGATTCCACGTAAAGGTGGTCAAGGTATGATTAAGTCAGATTTCTTTGTAATTAACAAAACTGATTTAg                                                              >  2:624617/1‑140 (MQ=255)
                                                                             cTTAGCAGCAACATTTAGTCCAGAACTTGTTGACTTTTCAATATATATTATCGATGTTGCTCGAGGTGAAAAGATTCCACGTAAAGGTGGTCAAGGTATGATTAAGTCAGATTTCTTTGTAATTAACAAAACTGATTTAg                                                              >  2:228155/1‑140 (MQ=255)
                                                                             cTTAGCAGCAACATTTAGTCCAGAACTTGTTGACTTTTCAATATATATTATCGATGTTGCTCAAGGTGAAAAGATTCCACGTAAAGGTGGTCAAGGTATGATTAAGTCAGATTTCTTTGTAATTAACAAAACTGATTTAg                                                              >  2:571455/1‑140 (MQ=255)
                                                                                       acctttAGTCCAGAACTTGTTGACATGTCAATATATATTATCGATGTTGCTTAAGGTGAATAGATTCCACGTAAAGGTGGTCAAGGTATGATTAAGTCAGATTTCTTTGTAATTAACAAAACTGATTTAGCTCCCTATGt                                                    <  1:203433/137‑1 (MQ=255)
                                                                                       aCATTTAGTCCAGAACTTGTTGACTTTTCAATATATATTATCGATGTTGCTCAAGGTGAAAAGATTCCACGTAAAGGTGGTCAAGGTATGATTAAGTCAGATTTCTTTGTAATTAACAAAACTGATTTAGCTCCCTATGt                                                    >  2:512115/1‑140 (MQ=255)
                                                                                         aTTTAGTTCAGAACTTTTTGACTTTTCAATATATATTATCGATGTTGCTCAAGGTGAAAAGATTCCACGTAAAGGTGGTCAAGGTATGATTAAGTCAGATTTCTTTGTAATTAACAAAACTGATTTAGCTCCCTATGTAg                                                  <  2:60228/140‑1 (MQ=255)
                                                                                         aTTTAGTCCAGAACTTGTTGACTTTTCAATATATATTATCGATGTTGCTCAAGGTGAAAAGATTCCACGTAAATGTGGTCAAGGTATGATTAAGTCAGATTTCTTTGTAATTAACAAAACTGATTTAGCTCCCTATGTAg                                                  <  1:268720/140‑1 (MQ=255)
                                                                                          tttAGTCCAGAACTTGTTGACTTTTCAATATATATTATCGATGTTGCTCAAGGTGAAAAGATTCCACGTAAAGGTGGTCAAGGTATGATTAAGTCAGATTTCTTTGTAATTAACAAAACTGATTTAGCTCCCTATGTAgg                                                 <  2:33954/140‑1 (MQ=255)
                                                                                          tttAGTCCAGAACTTGTTGACTTTTCAATATATATTATCGATGTTGCTCAAGGTGAAAAGATTCCACGTAAAGGTGGTCAAGGTATGATTAAGTCAGATTTCTTTGTAATTAACAAAACTGATTTAGCTCCCTATGTAgg                                                 <  2:757866/140‑1 (MQ=255)
                                                                                          tttAGTACAGAACCTGTTGACTTTTCAATATATATTATCGATGTTGCTCAAGGTGAAAAGATTCCACGTAAAGGTGGTCAAGGTATGATTAAGTCAGATTTCTTTGTAATTAACAAAACTGATTTAGCTCCCTATGTAgg                                                 <  2:113864/140‑1 (MQ=255)
                                                                                          gttAGTCCAGAACTTGTTGACTTTTCAATATATATTATCGATGTTGCTCAAGGTGAAAAGATTCCACGTAAAGGTGGTCAAGGTATGATTAAGTCAGATTTCTTTGTAATTAACAAAACTGATTTAGCTCCCTATGTAgg                                                 <  2:666115/139‑1 (MQ=255)
                                                                                           ttAGTCCAGAACTTGTTGACTTTTCAATATATATTATCGATGTTGCTCAAGATGAAAAGATTCCACGTAAAGGTGGTCAAGGTATGATTAAGTCAGATTTCTTTGTAATTAACAAAACTGATTTAGCTCACTATGTAGGt                                                >  1:420085/1‑140 (MQ=255)
                                                                                             aGTCCAGAACTTGTTGACTTTTCAATATATATTATCGATGTTGCTCAAGGTGAAAAGATTCCACGTAAAGGTGGTCAAGGTATGATTAAGTCAGATTTCTTTGTAATTAACAAAACTGATTTAGCTCCCTATGTAGGTGc                                              <  2:398661/140‑1 (MQ=255)
                                                                                             aGTCCAGAACTTGTTGACTTTTCAATATATATTATCGATGTTGCTCAAGGTGAAAAGATTCCACGTAAAGGTGGTCAAGGTATGATTAAGTCAGATTTCTTTGTAATTAACAAAACTGATTTAGCTCCCTATGTAGGTGc                                              >  1:10231/1‑140 (MQ=255)
                                                                                                 cAGAACTTGTTGACTTTTCAATATATATTATCGATGTTGCTCAAGGTGAAAAGATTCCACGTAAAGGTGGTCAAGGTATGATTAAGTCAGATTTCTTTGTAATTAACAAAACTGATTTAGCGCCCTATGTAGGTGcatca                                          <  1:695651/140‑1 (MQ=255)
                                                                                                   aaaCTTGTTGACTTTTCAATATATATTATCGATGTTGCTCAAGGTGAAAAGATTCCACGTAAAGGTGGTCAAGGTATGATTAAGTCAGATTTCTTTGTAATTAACAAAACTGATTTAGCTCCCTATGTAGGTGCATCAtt                                        <  2:630153/139‑1 (MQ=255)
                                                                                                      ctggttgACTGTTCAATATATATTATAGATGTTGCTCAAGGTGAAAAGATTCCACGTAGAGGTGGTCAAGGAAGTATTAAGACAGATTTCATTGTTATGAACAAAACTGATTTAGCTCCCTATGTCGGTGCATCATTAGa                                     >  2:376172/4‑140 (MQ=255)
                                                                                                      cTTGTTGACTTTTCAATATATATTATCGATGTTGCTCAAGGTGAAAAGATTCCACGTAAAGGTGGTCAAGGTATGATTAAGTCAGATTTCTTTGTAATTAACAAAACTGATTTAGCTCCCTATGTAGGTGCATCATTAGa                                     >  1:375167/1‑140 (MQ=255)
                                                                                                      cTTGTTGACATTTCAATATATATTATCGATGTTGCTCAAGGTGAAAACATTCCACGTAAAGGTGGTCAAGGTATGATTAAGTCAGATTTCTTTGTAATTAACAAAACTGATTTAGCTCCCTATGTAGGTGAATATTTAGa                                     >  2:488296/1‑140 (MQ=255)
                                                                                                        tgttgACTTTACAATATAAATTATCGAGGTTGCTCAAGGTGAAAAGATTCCACGTAAAGGTGGTCAAGGTATGATTAAGTCAGATTTCTTTGTAATTAACAAAACTGATTTAGCTCCCTATGTAGGTGCATCATTAGAAc                                   <  2:36760/140‑1 (MQ=255)
                                                                                                            gACTTTTCAATATATATTATCGATGTTGCTCAAGGTGAAAAGATTCCACGTAAAGGTGGTCAAGGTATGATTAAGTCAGATTTCTTTGTAATTAACAAAACTGATTTAGCTCCCTATGTAGGTGCATCATTAGAACAAAt                               >  2:576810/1‑140 (MQ=255)
                                                                                                            gACTTTTCAATATATATTATCGATGTTGCTCAAGGTGAAAAGATTCCACGTAAAGGTGGTCAAGGTATGATTAAGCCAGATTTCTTTGTTATCAACAAAACTGATTTAGCTCCCTATGTAGGTGCATCATTAGAACAAAt                               >  2:535386/1‑140 (MQ=255)
                                                                                                               ttttCAATATATATTATCGATGTTGCTCAAGGTGAAAAGATTCCACGTAAAGGTGGTCAAGGTATGATTAAGTCCGATTTCTTTGTAATTAACAAAACTGATTTAGCTCCCTATGTAGGTGCATCATTAGAACAAATGGc                            <  1:83675/140‑1 (MQ=255)
                                                                                                                  tCAATATATATTATCGATGTTGCTCAAGGTGAAAAGATTCCACGTAAAGGTGGTCAAGGTATGATTAAGTCAGATTTCTTTGTAATTAACAAAACTGATTTAGCTCCCTATGTAGGTGCATCATTAGAACaccccccgga                         >  2:725423/1‑131 (MQ=255)
                                                                                                                         atatTATCGATGTTGCTCAAGGTGAAAAGATTCCACGTAAAGGTGGTCAAGGTATGATTAAGTCAGATTTCTTTGTAATTAACAAAACTGATTTAGCTCCCTATGTAGGTGCATCATTAGAACAAATGGCTGAAGATACt                  >  2:704818/1‑140 (MQ=255)
                                                                                                                         atatTATCGATGATGCTCAAGGAGAAAAGATTCCACGTAAAGGCGGTCAAGGTATGATTAAGTCAGATTTCTTTGTAATTAACAAAACTGATTTAGCTCCCTATGTAGGTGCATCATTAGAACAAATGGCTGAAGATACt                  >  2:704991/1‑140 (MQ=255)
                                                                                                                              atCGATGTTGCTTAAGGTGAAAAGATTCCACGTAAAGGTGGTCAAGGTATGATTAAGTCAGATTTCTTTGTAATTAACAAAACTGATTTAGCTCCCTATGTAGGTGCATCATTAGAACAAATGGCTGAAGATACTAAAGt             <  1:753573/140‑1 (MQ=255)
                                                                                                                              atCGATGTTGCTCGAGGTGAAAAGATTCCACGTAAAGGTGGTCAAGGTATGATTAAGTCAGATTTCTTTGTAATTAACAAAACTGATTTAGCTCCCTATGTAGGTGCATCATTAGAACAAATGGCTGAAGATACTAAAGt             <  1:228155/140‑1 (MQ=255)
                                                                                                                              atCGATGTTGCTCGAGGTGAAAAGATTCCACGTAAAGGTGGTCAAGGTATGATTAAGTCAGATTTCTTTGTAATTAACAAAACTGATTTAGCTCCCTATGTAGGTGCATCATTAGAACAAATGGCTGAAGATACTAAAGt             <  1:624617/140‑1 (MQ=255)
                                                                                                                              atCGATGTTGCTCAAGGTGAAAAGATTCCACGTAAAGGTGGTCAAGGTATGATTAAGTCAGATTTCTTTGTAATTAACAAAACTGATTTAGCTCCCTATGTAGGTGCATCATTAGAACAAATGGCTGAAGATACTAAAGt             <  1:571455/140‑1 (MQ=255)
                                                                                                                                 gATGTTGCTCAAGGTGAAAAGATTCCACGTAAAGGTGGTCAAGGTATGATTAAGTCAGATTTCTTTGTAATTAACAAAACTGATTTAGCTCCCTATGTAGGTGCATCATTAGAACAAATGGCTGAAGATACTAAAGTAtt          >  1:422529/1‑140 (MQ=255)
                                                                                                                                       gCTCAAGGTGAAAAGATTCCACGTAAAGGTGGTCAAGGTATGATTAAGTCAGATTTCTTTGTAATTAACAAAACTGATTTAGCTCCCTATGTAGGTGCATCATTAGAACAAATGGCTGAAGATACTAAAGTATTTCGTgt    >  2:510944/1‑139 (MQ=255)
                                                                                                                                        cTCAAGGTGAAAAGATTCCACGTAAAGGTGGTCAAGGTATGATTAAGTCAGATTTCTTTGTAATTAACAAAACTGATTTAGCTCCCTATGTAGGTGCATCATTAGAACAAATGGCTGAAGATACTAAAGTATTTCGTGGt   >  1:217471/1‑140 (MQ=255)
                                                                                                                                        cTCAAGGTGAAAAGATTCCACGTAAAGGTGGTCAAGGTATGATTAAGTCAGATTTCTTTGTAATTAACAAAACTGATTTAGCTCCCTATGTAGGTGCATCATTAGAACAAATGGCTGAAGATACTAAAGTATTTCGTGGt   >  2:546834/1‑140 (MQ=255)
                                                                                                                                        cTCAAGGTGAAAAGATTCCACGTAAAGGTGGTCAAGGTATGATTAAGTCAGATTTCTTTGTAATTAACAAAACTGATTTAGCTCCCTATGTAGGTGCATCATTAAAACAAATGGCTGAAGATACTAAAGAATTTCATGGt   >  1:86059/1‑140 (MQ=255)
                                                                                                                                         tCAAGGTGAAAAGATTCCACGTAAAGGTGGTCAAGGTATGATTAAGTCAGATTTCTTTGTAATTAACAAAACTGATTTAGCTCCCTATGTAGGTGCATCATTAGAACAAATGGCTGAAGATACTAAAGTATTTCGTGGTa  >  2:432306/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                         
AACTTTGCAGCAATAGACGAATTATTAGAACGTCATGACGATATAGAACTAATTTTCATAGAATCTGGTGGCGATAACTTAGCAGCAACATTTAGTCCAGAACTTGTTGACTTTTCAATATATATTATCGATGTTGCTCAAGGTGAAAAGATTCCACGTAAAGGTGGTCAAGGTATGATTAAGTCAGATTTCTTTGTAATTAACAAAACTGATTTAGCTCCCTATGTAGGTGCATCATTAGAACAAATGGCTGAAGATACTAAAGTATTTCGTGGTA  >  CP000730/2409451‑2409727

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: