Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 110,651 C→T 10.5% D170D (GAC→GAT USA300HOU_0109 → staphylococcal tandem lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730110,6510CT10.5% 227.5 / 14.8 86D170D (GAC→GATUSA300HOU_0109staphylococcal tandem lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (32/44);  new base T (5/4);  total (38/48)
Fisher's exact test for biased strand distribution p-value = 4.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.14e-01

AAGATATCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAA  >  CP000730/110513‑110785
                                                                                                                                          |                                                                                                                                      
aaGATATCCGGTTAAAATGGTCAATAATAAAATCATTCCAACAAAAGAAATTAAAGATTAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACg                                                                                                                                       <  1:101946/140‑1 (MQ=255)
 agatttcttgTTAAATTTGTTTATAATAAAATGATTCAAACAAAAAAAATTAAATATGAAAAAATAAAAAAATAAATCGAAAACTTTAAGTTCTTTGTTCAATATGTCGACTTTAAAAATTTGAAAAATTATAAAGACgg                                                                                                                                      <  1:170190/131‑1 (MQ=18)
   atatCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTAAAGGACGTTGTTCAATAGGGCGACTTTAAAAATTTGAAAAATTATAAAGACGgag                                                                                                                                    >  1:552463/1‑140 (MQ=18)
    tatCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGgaga                                                                                                                                   <  1:63146/140‑1 (MQ=255)
    tatCCGGTTAAAATGGTAGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAGATAGAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAATATTTGAAAAATTATAAAGACGgaga                                                                                                                                   <  2:253960/140‑1 (MQ=255)
       ccGGTTAAAATGGTCGATAATAAAATCATTCAAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAATCTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGatat                                                                                                                                <  1:267501/140‑1 (MQ=255)
         ggTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATAttt                                                                                                                              <  1:739995/140‑1 (MQ=255)
              aactgttCGATAATTAAATCATTCCAAAAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCatat                                                                                                                         <  1:41221/134‑1 (MQ=255)
              aaaTGGTAGATAATAAAAACATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCatat                                                                                                                         <  1:221786/140‑1 (MQ=255)
                aTGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATaa                                                                                                                       <  1:162774/140‑1 (MQ=255)
                 tGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGTAATCGAAAACTTTAAGTGCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAAt                                                                                                                      <  1:196749/140‑1 (MQ=255)
                  ggTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATc                                                                                                                     <  1:657970/140‑1 (MQ=255)
                      aaaaataaAATCATTACAACAAAAGAAATTAAAGAAAAAAAAAAAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCaga                                                                                                                 <  2:661237/137‑1 (MQ=17)
                             aaaTCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCg                                                                                                          >  2:624696/1‑140 (MQ=255)
                             aaaTCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCAAAAGGTGACg                                                                                                          >  2:655045/1‑140 (MQ=255)
                               aTCATTCCAACAAAAGGAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAg                                                                                                        <  2:112713/140‑1 (MQ=255)
                               aTCATTCAAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAg                                                                                                        <  2:484329/140‑1 (MQ=255)
                                ttaTTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGt                                                                                                       <  2:734161/138‑1 (MQ=255)
                                tCATTCCAACAAAAAAAATTAAAAATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCCTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGt                                                                                                       <  2:58403/140‑1 (MQ=255)
                                   ttCCACCAAAATAAATTCAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTAt                                                                                                    <  1:476576/140‑1 (MQ=255)
                                      caacaaAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGAAGATATTTCATATAATACAGAGGTGCCGAGTTAttag                                                                                                 >  1:282134/1‑138 (MQ=255)
                                      caacaaAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATAGCAACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCg                                                                                                 >  1:388573/1‑140 (MQ=255)
                                       aacaaAAGAAATTAAAGATGAAAAAATAAAAAAGGAAATTGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCgg                                                                                                <  1:111471/140‑1 (MQ=255)
                                       aacaaAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAAAATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCgg                                                                                                >  2:387331/1‑140 (MQ=255)
                                       aacaaAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACGATAAGTACTTGGTTCAAAATGGCGACTTTAAAAATTTGAAAAATTATAAAGCCGGAGATATTTCTTATAATCCAGAGGTGCCGAGTTATTCgg                                                                                                >  2:57871/1‑140 (MQ=17)
                                        acaaAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAAATATTTCATATCATCAAGAGGTGCAGAAGTACTCGGc                                                                                               >  2:433725/1‑140 (MQ=17)
                                              gAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAata                                                                                         <  1:724852/140‑1 (MQ=255)
                                              gAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTAATATAATCCAGAGGTGCCGAGTTATTCGGCTAAata                                                                                         >  1:320387/1‑140 (MQ=255)
                                                aaTTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATAAAATCAAGAGTTGCCGAGTTATTTAGCTAAATATc                                                                                       >  1:420801/1‑140 (MQ=17)
                                                   tAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAAt                                                                                    >  2:137652/1‑140 (MQ=255)
                                                         tGAAAAAATAAAAAAAGAAATCGAAAACGTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTa                                                                              <  2:688849/140‑1 (MQ=255)
                                                             aaaaTAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAatga                                                                          <  2:714254/140‑1 (MQ=255)
                                                               aagaaaaaaaGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAACATTTGAAAAATTATAAAGACGGAGATATTTCTTATAATCTAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAatgatg                                                                        <  2:404973/137‑1 (MQ=255)
                                                               aaTAAAAAAAGAAATCTAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAatgatg                                                                        <  1:186600/140‑1 (MQ=255)
                                                               aaTAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGCAAAATTATAAAGACGGAGACATTTCATATAATCAAGAGATAGCGACTTATTCAGCTAAATATCAATTAACTaagtagg                                                                        >  2:334165/1‑135 (MQ=14)
                                                               aaTAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAatgatg                                                                        >  2:77894/1‑140 (MQ=255)
                                                               aaTAAAAAAAGAAATCGAAAACTTTAAGGTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAatgatg                                                                        <  2:702548/140‑1 (MQ=255)
                                                                aTAAAAAAAGAAATCTAAAACTTGAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCTAGTTATTCGGCTAAATATCAATTAACTAAtgatga                                                                       <  2:366045/140‑1 (MQ=255)
                                                                 tAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATgatgat                                                                      >  2:90290/1‑140 (MQ=255)
                                                                    aaaaaGAAATCGAAAACTTTAAGTTCTTTTTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATtat                                                                   <  2:236411/140‑1 (MQ=255)
                                                                    aaaaaGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATtat                                                                   >  2:660546/1‑140 (MQ=255)
                                                                    aaaaaGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATtat                                                                   >  1:169941/1‑140 (MQ=255)
                                                                    aaaaaGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATtat                                                                   >  1:429023/1‑140 (MQ=255)
                                                                    aaaaaGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGACGAGTTATTCGGCTAAATATCAATTAACTAATGATGATtat                                                                   >  1:90318/1‑140 (MQ=255)
                                                                           aaTCGAAAACTTTAAGTTCTTTGTTCAATATGGCGATTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTaa                                                            <  1:140923/140‑1 (MQ=255)
                                                                           aaTCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTaa                                                            <  2:663928/140‑1 (MQ=255)
                                                                             tCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTaaaa                                                          <  2:244064/140‑1 (MQ=255)
                                                                               gAAAACTTTAAGTTCTTTGTTCAATATGTCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACa                                                        >  2:367295/1‑140 (MQ=255)
                                                                               gAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAAcc                                                        >  2:227535/1‑139 (MQ=255)
                                                                               gAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACa                                                        >  2:655897/1‑140 (MQ=255)
                                                                               gAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACa                                                        >  1:473027/1‑140 (MQ=255)
                                                                               gAAAACTTTAAGTTCTCTGGTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGACATATTTCAGATACTCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACa                                                        <  2:30456/140‑1 (MQ=17)
                                                                                  accTTTAAGTGTTTTGTCCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAAtt                                                     <  2:370229/138‑1 (MQ=255)
                                                                                   aCTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTa                                                    <  2:715014/140‑1 (MQ=255)
                                                                                    cTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAAATATTTCATATAATCCAGAGGTGCCGAGTTATGCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTAc                                                   >  2:324875/1‑140 (MQ=255)
                                                                                    agttAAGTTCTTTGTTAAATATGGATACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTCCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGCTTATAATGTAAAACAATTAc                                                   <  1:459433/138‑1 (MQ=14)
                                                                                     tttAAGTTCTTTGTTCCATATCGCTACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCATAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACg                                                  <  2:174271/140‑1 (MQ=255)
                                                                                      ttAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGc                                                 >  1:733586/1‑140 (MQ=255)
                                                                                       tAAGTTCTTTTTTCAATATTGCGACTTGAAAAATTTGAAAAATTATAAATACGGTAATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCa                                                <  1:17399/140‑1 (MQ=17)
                                                                                       tAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCa                                                <  1:33530/140‑1 (MQ=255)
                                                                                       tAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCa                                                <  2:605639/140‑1 (MQ=255)
                                                                                         aGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCaaa                                              >  1:708075/1‑140 (MQ=255)
                                                                                                  ttCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAgatatgat                                     <  2:672675/140‑1 (MQ=255)
                                                                                                   tCAATATTTCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAgatatgata                                    <  2:330015/140‑1 (MQ=255)
                                                                                                           gCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACcgacg                            <  1:113829/140‑1 (MQ=255)
                                                                                                             gACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAg                          <  2:708075/140‑1 (MQ=255)
                                                                                                              aCTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATAATATACCGACGAGt                         >  2:135989/1‑140 (MQ=255)
                                                                                                                  tAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGAAAAAGATATGATATACCGACGAGTAAAg                     >  2:283748/1‑140 (MQ=255)
                                                                                                                   aaagatttaatgaaTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGc                    >  2:97491/13‑140 (MQ=11)
                                                                                                                    aagatttaatgaaTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCt                   >  2:168469/12‑140 (MQ=11)
                                                                                                                    aagatttaatgaaTTATAAAGATGGAGATATATCATATAATCCAGAGGAGCCGAGTTATTTGGCTAAATATCAATTAACTAATGATAATTATAATGTAAAACAATTACGCAAAAGTTAGGATATACAGACGAGTAAAGca                   >  2:76053/12‑139 (MQ=11)
                                                                                                                     agatttaatgaaTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTTATGATGATTATAATGTAAAACAGTTAGGCAAAAGATATGTTATACCGACGAGTGAAGATc                  >  1:670369/11‑140 (MQ=11)
                                                                                                                      gatttaatgaaTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTcc                 <  2:432989/131‑1 (MQ=14)
                                                                                                                      aaTTTGAAAAATTATAAAGAAGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGAGATGATATACCGACGAGTAAAGCTcc                 >  1:459731/1‑140 (MQ=255)
                                                                                                                        tttaatgaaTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCaa               <  2:414743/133‑1 (MQ=255)
                                                                                                                         ttGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAGATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGATCCaca              >  2:509634/1‑138 (MQ=255)
                                                                                                                             aaaaTTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTTCGCAAAAGATATAATTTACCCACGAGTAAAGCTCCAGAGttc          >  1:662675/1‑139 (MQ=17)
                                                                                                                             aaaaTTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTa          >  1:212007/1‑140 (MQ=255)
                                                                                                                             aaaaTTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTa          <  1:52389/140‑1 (MQ=255)
                                                                                                                              aaaTTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTAt         <  1:640083/140‑1 (MQ=255)
                                                                                                                                  tataAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGtt     <  1:467032/140‑1 (MQ=255)
                                                                                                                                    taAAGATGGAGATAAATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTaa   <  2:32690/140‑1 (MQ=255)
                                                                                                                                     aaaGATGGAGATATATCATATAATACAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCACCAAAGTTATTGTTaaa  >  1:346356/1‑140 (MQ=17)
                                                                                                                                     aaaGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTaaa  >  2:655310/1‑140 (MQ=255)
                                                                                                                                     aaaGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTaaa  <  1:408387/140‑1 (MQ=255)
                                                                                                                                     aaaGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTaaa  >  2:385719/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                      
AAGATATCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAA  >  CP000730/110513‑110785

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: