Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 111,512 A→G 33.9% S194S (TCA→TCG USA300HOU_0110 → possible staphylococcal tandem lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730111,5120AG33.9% 109.2 / 74.5 96S194S (TCA→TCGUSA300HOU_0110possible staphylococcal tandem lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (35/28);  new base G (16/17);  total (51/45)
Fisher's exact test for biased strand distribution p-value = 5.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAA  >  CP000730/111376‑111649
                                                                                                                                        |                                                                                                                                         
cTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCt                                                                                                                                        <  2:693024/140‑1 (MQ=255)
  gAGGAAGTTAAAGAACAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCa                                                                                                                                      <  2:64200/140‑1 (MQ=255)
   aGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATACAGAGGTGCCGAGTTATTCAGCTCaa                                                                                                                                     >  1:34743/1‑140 (MQ=255)
    ggAAGTTAAAGAAGAAAACATAAAAAAAGCAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAat                                                                                                                                    <  2:484926/140‑1 (MQ=255)
    ggAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAat                                                                                                                                    <  1:314481/140‑1 (MQ=255)
    ggAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAat                                                                                                                                    <  1:15685/140‑1 (MQ=255)
    ggAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAat                                                                                                                                    <  2:622326/140‑1 (MQ=255)
     gAAGTTAAAGAAGAAAACATAAAAAAATTAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAata                                                                                                                                   <  1:625373/140‑1 (MQ=255)
     gAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGATGCCGAGTTATTCAGCTCAata                                                                                                                                   >  1:677887/1‑140 (MQ=255)
      aaattAAAGAAGAAAACATAAAAAAAGAAATCGAATACTTTTAGTTTTTTGCGCAATATAGTAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAatat                                                                                                                                  <  1:102967/137‑1 (MQ=17)
      aaGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATATTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATACAGAGGTGCCGAGTTATTAAGCTCAatat                                                                                                                                  >  2:613663/1‑140 (MQ=255)
       aGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGAAGATATATCATATAATTCAGAGATACCGAGTTATTCAGCTCAATATc                                                                                                                                 >  1:245854/1‑140 (MQ=255)
        gTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCACTATAGCAATTTTAAAGATTTAATGAATTATAAAGAAGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCACAATATCa                                                                                                                                <  2:561111/140‑1 (MQ=255)
        gTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCa                                                                                                                                <  1:521684/140‑1 (MQ=255)
        gTTAAAGAAGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGGGATAAATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCa                                                                                                                                <  1:146543/140‑1 (MQ=255)
         ttAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCaa                                                                                                                               >  2:26318/1‑140 (MQ=255)
          tAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGGAATTTTAAAGATTTAAGGAGTTAAAAAGATGGAGATATATCATATAATACAGAGGTGGCGAATTGTTCGGTGAAATATCAAt                                                                                                                              >  2:201882/1‑140 (MQ=17)
          tAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAAt                                                                                                                              <  2:399235/140‑1 (MQ=255)
           aaaGAAGAGAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCATTTTAGCAATTTTAAAGATTTTATGATTTTTTATGTTGGTGGTATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAAtt                                                                                                                             <  1:56476/140‑1 (MQ=17)
           aaaGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTACCGAGTTATTCGGCTAAATATCAAtt                                                                                                                             >  1:274761/1‑140 (MQ=255)
            aagaagAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTa                                                                                                                            >  1:467069/1‑140 (MQ=255)
              gaagaaAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAAc                                                                                                                          <  1:628576/140‑1 (MQ=255)
              gaagaaAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAAc                                                                                                                          <  1:73057/140‑1 (MQ=255)
              gaagaaAACATAAAAAAAGAAATCGAAAACTTTAAGTCTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAAc                                                                                                                          >  2:124687/1‑140 (MQ=255)
                 gaaAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCTAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTaa                                                                                                                       >  2:720974/1‑140 (MQ=255)
                 gaaAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAACATGGAGATATATCATATAATCCAGAGATGCCGAGTTATTCGGCTCAATATCAATTAACTaa                                                                                                                       >  1:485741/1‑140 (MQ=255)
                  aaaaCATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAat                                                                                                                      <  2:656231/140‑1 (MQ=255)
                  aaaaCATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATAAATTAACTAat                                                                                                                      >  1:762197/1‑140 (MQ=255)
                  aaaaCATAAAAAAAGAAATCGAAAACTGTAAGGTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAat                                                                                                                      <  2:727646/140‑1 (MQ=18)
                    aaCATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTTCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATACGGCGAAATATCAATTAAATAatga                                                                                                                    >  2:199593/1‑140 (MQ=17)
                      cATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAatgatg                                                                                                                  <  2:96582/140‑1 (MQ=255)
                        tAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATgatgat                                                                                                                >  1:432989/1‑140 (MQ=255)
                           aaaaaGAAATCGAAAACTTTACGTTTTTTGCGCAATATAGCTATTTTAAAGATTTAATGAATTATAAATATGGAGATATATAATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATtat                                                                                                             <  1:217898/140‑1 (MQ=255)
                           aaaaaGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATtat                                                                                                             <  1:433265/140‑1 (MQ=255)
                             aaaGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATaa                                                                                                           <  2:481891/140‑1 (MQ=255)
                              aataaaTCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAATGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCATGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAAt                                                                                                          <  2:125613/137‑1 (MQ=255)
                              aaGAAATCGAAAACTTTAAGTTTTTTGCGCAATATACAAATTATAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAAt                                                                                                          <  1:326810/140‑1 (MQ=255)
                                gAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTGATGATGATTATAATGt                                                                                                        >  1:510111/1‑140 (MQ=255)
                                gAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGt                                                                                                        >  2:153652/1‑140 (MQ=255)
                                  aaTCGAATACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTCAATGAATCATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATCATGATTATAATGTaa                                                                                                      <  1:561361/140‑1 (MQ=17)
                                  aaTCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATAGCAATTAACTAATGATGATTATAATGTaa                                                                                                      <  1:149739/140‑1 (MQ=255)
                                  aaTCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTACAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTaa                                                                                                      <  2:502279/140‑1 (MQ=255)
                                   aTCGAAAACTTTAATTTTTTTGCGAAATATAACAATTTTAAAGATTTAATGAATTATAAAGATAGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTaaa                                                                                                     <  2:376155/140‑1 (MQ=255)
                                   aTCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTaaa                                                                                                     <  2:697153/140‑1 (MQ=255)
                                    tCGAAAACTTTAAGTTTTTTGCTCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTaaaa                                                                                                    >  2:394502/1‑140 (MQ=255)
                                    tCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTaaaa                                                                                                    <  1:433696/140‑1 (MQ=255)
                                      gAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTaaacaa                                                                                                  >  2:564772/1‑137 (MQ=255)
                                      gAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACa                                                                                                  >  2:263803/1‑140 (MQ=255)
                                        aaaCTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAAATATAAAGAAGGTTAAAGATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAAt                                                                                                <  1:748335/140‑1 (MQ=18)
                                         aaCTTTAAGTTTTTTGCGCAATATAGTAATTTTAAAGATTTGATGTATTATAAAGTTGGAGATATATCATATAATCTAGAGGTGCCGAGTTATTCAGCTCAATATCAATTATTTAATGATGATTATAATGTAAAACAttt                                                                                               >  1:207802/1‑140 (MQ=18)
                                         aaCTTTAAGTTCTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAAtt                                                                                               <  2:187935/140‑1 (MQ=255)
                                              tAAGTTTTTTGCGCAAGATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACgca                                                                                          >  2:542851/1‑138 (MQ=255)
                                                agtttttttttttttattGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTAAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACTTaaa                                                                                        >  2:84511/15‑140 (MQ=18)
                                                 gTTTTTTTCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCACAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTaaaa                                                                                       <  1:201567/140‑1 (MQ=255)
                                                           gaaTATAGCAATCTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACAt                                                                             <  1:62493/139‑1 (MQ=255)
                                                                 aGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATAccgac                                                                       <  2:431083/140‑4 (MQ=11)
                                                                 aGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACcaac                                                                       <  1:524311/140‑1 (MQ=255)
                                                                   taaTTTTAAACATTTAATGAATTATAAAGATTGATATATATCATATAATCCAGAGGTGCCGAGTTATTCATCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACcaacaa                                                                     <  1:70535/139‑1 (MQ=255)
                                                                   cAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACcaacaa                                                                     <  1:75404/140‑1 (MQ=255)
                                                                    aaTTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATCTCAATTAACTAATGATGATTATAATGTAAAACACTTACGCAAAAGATATgctataacgatggt                                                                    >  2:716938/1‑127 (MQ=14)
                                                                    aaTTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACcgacgag                                                                    >  1:639605/1‑137 (MQ=11)
                                                                    aaTTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACaaa                                                                    <  1:702648/140‑1 (MQ=255)
                                                                       tttAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCGAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACcgacgagaaa                                                                 >  2:572793/1‑134 (MQ=11)
                                                                           aaGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAgcg                                                             >  1:413645/1‑140 (MQ=255)
                                                                            aGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCAACGAATAAAgctc                                                            >  1:288971/1‑138 (MQ=11)
                                                                               tttAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATAACATACCAACAAATAAAGCTCCGa                                                         >  2:505847/1‑140 (MQ=255)
                                                                               tttAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTAAGTAAAAGATATGACATACCAACAAATAAAGCGCCGa                                                         >  2:298759/1‑140 (MQ=255)
                                                                               tttAATGAATTATAAAGATGGAGATATATCATAGAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATAACATACCAACAAATAAAGCGCCGa                                                         >  1:753876/1‑140 (MQ=255)
                                                                                  aaTGAATTATAAACATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGCTGATTATAATGTAAAACAATTACGCTAAAGATATGACATACCAACAAACAACGCGGCGACGc                                                      >  2:751029/1‑140 (MQ=17)
                                                                                   aTGAATTATAAAGATGGAGATATATCATATAATCCAGAGGCGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAGGCt                                                     >  1:650647/1‑140 (MQ=255)
                                                                                           taAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTAATGAGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGAAATACCAAAAAATAACGCAACGAAGCTGTTGTGGa                                             >  2:498684/1‑140 (MQ=14)
                                                                                             gaGATGGAAATATATCATATAATCCAGGGGTGGCAAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGaaa                                           <  2:690998/139‑1 (MQ=255)
                                                                                             acgATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATAAAGCGCAATATCAATTAACTAATGATGATTATAATATAAAACAATTACGTCACAGATATGACATGCAAACAAATAAAGCACCGAAACCGTTGATGcaa                                           >  2:318845/3‑140 (MQ=14)
                                                                                              aGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAg                                          >  2:591425/1‑140 (MQ=255)
                                                                                              aGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGAAAAAGATATGACATACAAACAAATAAAGCAACAAAGCTATTATTGAAAg                                          >  1:473743/1‑140 (MQ=14)
                                                                                              aGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATGACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAg                                          >  2:561274/1‑140 (MQ=255)
                                                                                                     gaTATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAgg                                   >  1:726760/1‑140 (MQ=255)
                                                                                                        atatCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGaa                                <  1:726488/140‑1 (MQ=255)
                                                                                                        atatCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGaa                                <  1:595883/140‑1 (MQ=255)
                                                                                                        atatCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGATGAAAGGTACAGGGaa                                >  1:128281/1‑140 (MQ=255)
                                                                                                        atatCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGAAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGaa                                >  1:193226/1‑140 (MQ=255)
                                                                                                                 aaTCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAACGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAgg                       >  1:55692/1‑140 (MQ=255)
                                                                                                                  aTCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGt                      >  1:155999/1‑140 (MQ=255)
                                                                                                                   tCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGtt                     >  1:711429/1‑140 (MQ=255)
                                                                                                                    tcAGAGGTGCCGTGTGAGTCGGCGAAATATCAATTAACTAATGATGATTATGAGGTAAAAGAATTACGTAAAAGTTATGAGAAACCCACAAAAGGAGCGCCGAAGCTGTTGTGGAAAGGTACAGGGCATTTAAAAGGTtc                    <  2:485741/139‑1 (MQ=2)
                                                                                                                     caaagGTGCCGAATTATTCAGCTCAATATCAATTAACTAATGAAGAATATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtca                   <  1:720974/137‑1 (MQ=255)
                                                                                                                     cAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtca                   >  2:362590/1‑140 (MQ=255)
                                                                                                                      agagGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtcat                  >  1:206617/1‑140 (MQ=255)
                                                                                                                       gagGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtcatc                 >  1:43364/1‑140 (MQ=255)
                                                                                                                       gagGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAACTTAAAAGGTtcatg                 >  2:716439/1‑139 (MQ=255)
                                                                                                                       gagGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTGGAAAGGTACAGGGAAATTAAAAGGTtcctc                 >  2:759849/1‑140 (MQ=255)
                                                                                                                          gTGCGGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGAACAGGGAATTTAGAAGGTTCATCAGt              >  1:542835/1‑140 (MQ=255)
                                                                                                                               gAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTGAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGat         >  2:660496/1‑140 (MQ=255)
                                                                                                                               gAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATAAGTTGGaa         >  2:37144/1‑139 (MQ=255)
                                                                                                                                    aatCAACTCAATAACAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGAAATGACATACCAACAAATAAAGCGCCGAAGCTGATGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATaaa    <  1:227963/138‑1 (MQ=14)
                                                                                                                                      tCAGCTAAATATCAATTAACTAATGATGATTATAATTTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATaaaaa  <  2:245854/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                         
CTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAA  >  CP000730/111376‑111649

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: