| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP000730 | 1,064,254 | Δ1 bp | coding (235/1218 nt) | brpA ← | LytR family transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 1,064,253 | 0 | C | . | 96.2% | 116.7 / ‑1.7 | 26 | coding (236/1218 nt) | brpA | LytR family transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base . (14/11); total (14/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.62e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01 | |||||||||||
TGCATGGGTAATCTTATCGTAATAGCCAACTTTAGGAATGTAGCTGATAGTATCACGTGGAATACTAAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGTCCTTTTTTTCTTCTTCCATCGTTATCATCGATACCTAAGAAAAGAATAGAGATAGGTTGTTCTTCGGGATTGACTTTATTATCTCTTAAGTTGGATTGACGATTAGCATTTTTGCTGTCTTGAG > CP000730/1064108‑1064376 | tgcatgGGTAATCTTATCGTAATAGCCAACTTTAGGAATGTAGCTGATAGTATCACGTGGAATACTAAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGTCttt < 1:64263/149‑1 (MQ=255) tAGCCAACTGTAGGAAGGTAGCTTAGAGTATCACGGGGTATACTAATCATTCTAATTTGATGTTTTAATTGATTCAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCTTCCTGT‑CTTTTTTTCTTCTTCCATCTTTatct > 2:119581/1‑148 (MQ=255) aGCCAACTTTAGGAATGTAGCTGATAGTATCACGTGGAATACTAAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGT‑CTTTTTTTCTTCTTCCATCGTTatcat < 1:263162/149‑1 (MQ=255) aGCCAACTTTAGGAATGTAGCTGATAGTATCACGTGGAATACTAAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGT‑CTTTTTTTCTTCTTCCATCGTTatcat > 1:320413/1‑149 (MQ=255) cAACTTTAGGAATGTAGCTGATAGTATCACGTGGAATACTAAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGT‑CTTTTTTTCTTCTTCCATCGTTATCATCGa > 1:292992/1‑149 (MQ=255) cAACTTTAGGAATGTAGCTGATAGTATCACGTGGAATACTAAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGT‑CTTTTTTTCTTCTTCCATCGTTATCATCGa > 1:325939/1‑149 (MQ=255) cTTTAGGACTGTAGCTGATAGTATCACGTGGAATACTCAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATTGCGTCTGATCTAGAGTGTTCCGCATCCTGT‑CTTATTTTCTTCTACCATCGTTATCATCGATAc < 2:220316/149‑1 (MQ=255) cTTTAGGAATGTAGCTGATAGTATCACGTGGAATACTAAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGT‑CTTTTTTTCTTCTTCCATCGTTATCATCGATAc < 1:307751/149‑1 (MQ=255) tttAGGAATGTAGCTGATAGTATCACGTGGAATACTAAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGT‑CTTTTTTTCTTCTTCCATCGTTATCATCGATAcc < 1:247263/149‑1 (MQ=255) tttAGGAATGTAGCTGATAGTATCACGTGGAATACTAAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGT‑CTTTTTTTCTTCTTCCATCGTTATCATCGATAcc < 2:85103/149‑1 (MQ=255) tttAGGAATGTAGCTGATAGTATCACGTGGAATACTAAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGT‑CTTTTTTTCTTCTTCCATCGTTATCATCGATAcc < 1:58249/149‑1 (MQ=255) gAATGTAGCTGATAGTATCACGTGGAATACTAAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGT‑CTTTTTTTCTTCTTCCATCGTTATCATCGATACCTaaga > 2:208791/1‑149 (MQ=255) aaTGTAGCTGATAGTATCACGTGGAATACTAAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGT‑CTTTTTTTCTTCTTCCATCGTTATCATCGATACCTaagaa > 2:214431/1‑149 (MQ=255) cttaTAGTATCACGTGGAATACTAAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGT‑CTTTTTTTCTTCTTCCATCGTTATCATCGATACCTAAGAAAAGAATa > 1:121684/4‑149 (MQ=255) cTAAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGT‑CTTTTTTTCTTCTTCTATCGTTATCATCGATACCTAAGAAAAGAATAGAGATAGGTTGTTCTTCGGGa > 2:127064/1‑149 (MQ=255) cTAAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGT‑CTTTTTTTCTTCTTCCATCGTTATCATCGATACCTAAGAAAAGAATAGAGATAGGTTGTTCTTCGGGa < 2:251940/149‑1 (MQ=255) gCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGT‑CTTTTTTTCTTCTTCCATCGTTATCATCGATACCTAAGAAAAGAATAGAGATAGGTTGTTCTTCGGGATTGa > 2:112343/1‑149 (MQ=255) cATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGT‑CTTTTTTTCTTCTTCCATCGTTATCATCGATACCTAAGAAAAGAATAGAGATAGGTTGTTCTTCGGGATTGAc > 1:123770/1‑149 (MQ=255) tAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGT‑CTTTTTTTCTTCTTCCATCGTTATCATCGATACCTAAGAAAAGAATAGAGATAGGTTGTTCTTCGGGATTGACTttat < 1:107236/149‑1 (MQ=255) ttgattAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGT‑CTTTTTTTCTTCTTCCATCGTTATCATCGATACCTAAGAAAAGAATAGAGATAGGTTGTTCTTCGGGATTGACTTTATTATCTCTTAAGTTGGa > 1:163586/1‑149 (MQ=255) aaaaGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGT‑CTTTTTTTCTTCTTCCATCGTTATCATCGATACCTAAGAAAAGAATAGAGATAGGTTGTTCTTCGGGATTGACTTTATTATCTCTTAAGTTGGATTGACg < 2:309359/149‑1 (MQ=255) aTCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGT‑CTTTTTTTTTTTTTTCATCGTTATCATCGACACATAAGAAAAGAATAGTGATAGGTTGTTCTTCGGGTTTTACTTTATTATCTCTTAATTTGGTTTGACGATTAGCATTTTTg > 2:132201/1‑149 (MQ=255) gCGTCTGATCTAGAGTGTTCAGCATCCTGT‑CTTTTTTTCTTCTTCCATCGTTATCATCGATACCTAAGAAAAGAATAGAGATAGGTTGTTCTTCGGGATTGACTTTATTATCTCTTAAGTTGGATTGACGATTAGCATTTTTGCTGttt > 1:12102/1‑147 (MQ=255) gCGTCTGATCTAGAGTGTTCAGCATCCTGT‑CTTTTTTTCTTCTTCCATCGTTATCATCGATACCTAAGAAAAGAATAGAGATAGGTTGTTCTTCGGGATTGACTTTATTATCTCTTAAGTTGGATTGACGATTAGCATTTTTGCTGTCt < 1:294117/149‑1 (MQ=255) cGTCTGATCTAGAGTGTTCAGCATCCTGT‑CTTTTTTTCTTCTTCCATCGTTATCATCGATACCTAAGAAAAGAATAGAGATAGGTTGTTCTTCGGGATTGACTTTATTATCTCTTAAGTTGGATTGACGATTAGCATTTTTGCTGTCtt > 1:176406/1‑149 (MQ=255) cTGATCTAGAGTGTTCAGCATCCTGT‑CTTTTTTTCTTCTTCCATCGTTATCATCGATACCTAAGAAAAGAATAGAGATAGGTTGTTCTTCGGGATTGACTTTATTATCTCTTAAGTTGGATTGACGATTAGCATTTTTGCTGTCTTgag < 1:214431/149‑1 (MQ=255) | TGCATGGGTAATCTTATCGTAATAGCCAACTTTAGGAATGTAGCTGATAGTATCACGTGGAATACTAAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGTCCTTTTTTTCTTCTTCCATCGTTATCATCGATACCTAAGAAAAGAATAGAGATAGGTTGTTCTTCGGGATTGACTTTATTATCTCTTAAGTTGGATTGACGATTAGCATTTTTGCTGTCTTGAG > CP000730/1064108‑1064376 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |