Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	3,790	G→A	A32T (GCA→ACA)	yaaA →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	3,790	0	G	A	100.0%	148.1 / NA	40	A32T (GCA→ACA)	yaaA	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/11);  total (29/11)

AAAGGTATAATATATTAATGACATACAAAGAAATGGAGTGATTATTTTGGTTCAAGAAGTTGTAGTAGAAGGAGACATTAATTTAGGTCAATTTCTAAAAACAGAAGGGATTATTGAATCTGGTGGTCAAGCAAAATGGTTCTTGCAAGACGTTGAAGTATTAATTAATGGAGTGCGTGAAACACGTCGCGGTAAAAAGTTAGAACATCAAGATCGTATAGATATCCCAGAATTACCTGAAGATGCTGGTTCTTTCTTAATCATTCATCAAGGTG  >  CP000730/3660‑3934                                                                                                                                   |                                                                                                                                                 aaaGGTATAATATATTAATGACATACAAAGAAATGGAGTGATTATTTTGGTTCAAGAAGTTGTAGTAGAAGGAGACATTAATTTAGGTCAATTTCTAAAAACAGAAGGGATTATTGAATCTGGTGGTCAAACAAAATGGTTCTTGCAAg                                                                                                                                >  2:277210/1‑149 (MQ=255) aaaGGTATAATATATTAATGACATACAAAGAAATGGAGTGATTATTTTGGTTCAAGAAGTTGTAGTAGAAGGAGACATTAATTTAGGTCAATTTCTAAAAACAGAAGGGATTATTGAATCTGGTGGTCAAACAAAATGGTTCTTGCAAg                                                                                                                                >  1:107907/1‑149 (MQ=255)      tataATATATTAATGACATACAAAGAAATGGAGTGATTATTTTGGTTCAAGAAGTTGTAGTAGAAGGATACATTAATTTAGGTCAATTTCTAAAAACAGAAGGGATTATTGAATCTGGTGGTCAAACAAAATGGTTCTTGCAAGACGtt                                                                                                                           <  1:270621/149‑1 (MQ=255)      tataATATATTAATGACATACAAAGAAATGGAGTGATTATTTTGGTTCAAGAAGTTGTAGTAGAAGGAGACATTAATTTAGGTCAATTTCTAAAAACAGAAGGGATTATTGAATCTGGTGGTCAAACAAAATGGTTCTTGCAAGACGtt                                                                                                                           <  1:270631/149‑1 (MQ=255)           tatatTAATGACATACAAAGAAATGGAGTGATTATTTTGGTTCAAGAAGTTGTAGTAGAAGGAGACATTAATTTAGGTCAATTTCTAAAAACAGAAGGGATTATTGAATCTGGTGGTCAAACAAAATGGTTCTTGCAAGACGTTGAAGt                                                                                                                      >  1:38853/1‑149 (MQ=255)           tatatTAATGACATACAAAGAAATGGAGTGATTATTTTGGTTCAAGAAGTTGTAGTAGAAGGAGACATTAATTTAGGTCAATTTCTAAAAACAGAAGGGATTATTGAATCTGGTGGTCAAACAAAATGGTTCTTGCAAGACGTTGAAGt                                                                                                                      >  1:52235/1‑149 (MQ=255)            atatTAATGACATACAAAGAAATGGAGTGATTATTTTGGTTCAAGAAGTTGTAGTAGACGGAGACATTAATTTATGTCAATTTCTAAAAACAAAAGGGATTATTTAATCTGGTGGTCAAACAAAATGGATCTTGCAAGACGTTGAAGTa                                                                                                                     >  2:301963/1‑149 (MQ=255)             tatTAATGACATACAAAGAAATGGAGTGATTATTTTGGTTCAAGAAGTTGTAGTAGAAGGAGACATTAATTTAGGTCAATTTCTAAAAACAGAAGGGATTATTGAATCTGGTGGTCAAACAAAATGGTTCTTGCAAGACGTTGAAGTat                                                                                                                    <  2:146865/149‑1 (MQ=255)              atTAATGACATACAAAGAAATGGAGTGATTATTTTGGTTCAAGAAGTTGTAGTAGAAGGAGACATTAATTTAGGTCAATTTCTAAAAACAGAAGGGATTATTGAATCTGGTGGTCAAACAAAATGGTTCTTGCAAGACGTTGAAGTatt                                                                                                                   <  2:161876/149‑1 (MQ=255)                tAATGACATACAAAGAAATGGAGTGATTATTTTGGTTCAAGAAGTTGTAGTAGAAGGAGACATTAATTTAGGTCAATTTCTAAAAACAGAAGGGATTATTGAATCTGGTGGTCAAACAAAATGGTTCTTGCAAGACGTTGAAGTattaa                                                                                                                 >  2:89858/1‑149 (MQ=255)                           aaaGAAATGGAGTGATTATTTTGGTTCAAGAAGTTGTAGTAGAAGGAGACATTAATTTAGGTCAATTTCTAAAAACAGAAGGGATTATTGAATCTGGTGGTCAAACAAAATGGTTCTTGCAAGACGTTGAAGTATTAATTAATGGAGTg                                                                                                      >  1:140156/1‑149 (MQ=255)                             aGAAATGGAGTGATTATTTTGGTTCAAGAAGTTGTAGTAGAAGGAGACATTAATTTAGGTCAATTTCTAAAAACAGAAGGGATTATTGAATCTGGTGGTCAAACAAAATGGTTCTTGCAAGACGTTGAAGTATTAATTAATGGAGTGCg                                                                                                    >  2:22219/1‑149 (MQ=255)                                         attattTTGGTTCAAGAAGTTGTAGTAGAAGGAGACATTAATTTAGGTCAATTTCGAAAAACAGAAGGGATTATGGAATCTGGTGGTCAAACAAAATGGTTCTCGCAAGACGTTGAAGTATTAATTAATGGAGTGCGTGAAACACGTcg                                                                                        >  1:100147/1‑149 (MQ=255)                                          ttattTTGGTTCAAGAAGTTGTAGTAGAAGGAGACATTAATTTAGGTCAATTTCTAAAAACAGAAGGGATTATTGAATCTGGTGGTCAAACAAAATGGTTCTTGCAAGACGTTGAAGTATTAATTAATGGAGTGCGTGAAACACGTcgc                                                                                       >  1:110113/1‑149 (MQ=255)                                              tttGGTTCAAGAAGTTGTAGTAGAAGGAGACATTAATTTAGGTCAATTTCTAAAAACAGAAGGGATTATTGAATCTGGTGGTCAAACAAAATGGTTCTTGCAAGACGTTGAAGTATTAATTAATGGAGTGCGTGAAACACGTCGCGGTa                                                                                   >  1:103048/1‑149 (MQ=255)                                                    tCAAGAAGTTGTAGTAGAAGGAGACATTAATTGAGGTCAATTTCTAAAAACAGAAGGGATTATTGAATCTGGTGGTCAAACAAAATGGTTCTTGCAAGACGTTGAAGTATTAATTAATGGAGTGCGTGAAACACGTCGCGGTAAAAAGt                                                                             >  2:240406/1‑149 (MQ=255)                                                         aagTTGTAGTAGAAGGAGACATTAATTTAGGTCAATTTCTAAAAACAGAAGGGATTATTGAATCTGGTGGTCAAACAAAATGGTTCTTGCAAGACGTTGAAGTATTAATTAATGGAGTGCGTGAAACACGTCGCGGTAAAAAGTTAGaa                                                                        <  2:79248/149‑1 (MQ=255)                                                         aagTTGTAGTAGAAGGAGACATTAATTTAGGTCAATTTCTAAAAACAGAAGGGATTATTGAATCTGGTGGTCAAACAAAATGGTTCTTGCAAGACGTTGAAGTATTAATTAATGGAGTGCGTGAAACACGTCGCGGTAAAAAGTTAGaa                                                                        >  2:212652/1‑149 (MQ=255)                                                             tGTAGTAGAAGGAGACATTAATTTAGGTCAATTTCTAAAAACAGAAGGGATTATTGAATCTGGTGGTCAAACAAAATGGTTCTTGCAAGACGTTGAAGTATTAATTAATGGAGTGCGTGAAACACGTCGCGGTAAAAAGTTAGAACATc                                                                    >  1:7186/1‑149 (MQ=255)                                                                    gAAGGAGACATTAAGTTAGGTCAATTTCTAAAAACAGAAGGGATTATTGAACCTGGTGGTCAAACAAAATGGTTCTTGCAAGACGTTGAAGTATTAATTAATGGAGTGCGTGAAACACGTCGCGGTAAAAAGTTAGAACATCAAGATCg                                                             <  1:117384/149‑1 (MQ=255)                                                                         agaCATTAATTTAGGTCAATTTCTAAAAACAGAAGGGATTATTGAATCTGGTGGTCAAACAAAATGGTTCTTGCAAGACGTTGAAGTATTAATTAATGGAGTGCGTGAAACACGTCGCGGTAAAAAGTTAGAACATCAAGATCGTATAg                                                        >  2:174708/1‑149 (MQ=255)                                                                                 aTTTAGGTCAATTTCTAAAAACAGAAGGGATTATTGAATCTGGTGGTCAAACAAAATGGTTCTTGCAAGACGTTGAAGTATTAATTAATGGAGTGCGTGAAACACGTCGCGGTAAAAAGTTAGAACATCAAGATCGTATAGATATCCCa                                                >  1:237870/1‑149 (MQ=255)                                                                                 aTTTAGGTCAATTTCTAAAAACAGAAGGGATTATTGAATCTGGTGGTCAAACAAAATGGTTCTTGCAAGACGTTGAAGTATTAATTAATGGAGTGCGTGAAACACGTCGCGGTAAAAAGTTAGAACATCAAGATCGTATAGATATCCCa                                                >  1:242264/1‑149 (MQ=255)                                                                                 aTTTAGGTCAATTTCTAAAAACAGAAGGGATTATTGAATCTGGTGGTCAAACAAAATGGTTCTTGCAAGACGTTGAAGTATTAATTAATGGAGTGCGTGAAACACGTCGCGGTAAAAAGTTAGAACATCAAGATCGTATAGATATCCCa                                                >  2:310015/1‑149 (MQ=255)                                                                                  tttAGGTCAATTTCTAAAAACAGAAGGGATTATTGAATCTGGTGGTCGAACAAAATGGTTCTTGCAAGACGTTGAAGTATTAATTAATGGAGTGCGTGAAACACGTCGCGGTAAAAAGTTAGAACATCAAGATCGTATAGATATCCCAg                                               <  1:233774/149‑1 (MQ=255)                                                                                   ttAGGTCAATTTCTAAAAACAGAAGGGATTATTGAATCTGGTGGTCAAACAAAATGGTTCTTGCAAGACGTTGAAGTATTAATTAATGGAGTGCGTGAAACACGTCGCGGTAAAAAGTTAGAACATCAAGATCGTATAGATATCCCAGa                                              >  2:59178/1‑149 (MQ=255)                                                                                    tAGGTCAATTTCTAAAAACAGAAGGGATTATTGAATCTGGTGGTCAAACAACATCATTCTTGCAAGACGTTGAAGTATTAATTAATGGAGTGCGTGAAACACGTCGCGGTAAAAAGTTAGAACATCAAGATCGTATAGATATCCCAGaa                                             <  1:277210/149‑1 (MQ=255)                                                                                    tAGGTCAATTTCTAAAAACAGAAGGGATTATTGAATCTGGTGGTCAAACAAAATGGTTCTTGCAAGACGTTGAAGTATTAATTAATGGAGTGCGTGAAACACGTCGCGGTAAAAAGTTAGAACATCAAGATCGTATAGATATCCCAGaa                                             <  2:107907/149‑1 (MQ=255)                                                                                       gTCAATTTCTAAAAACAGAAGGGATTATTGAATCTGGTGGTCAAACAAAATGGTTCTTGCAAGACGTTGAAGTATTAATTAATGGAGTGCGTGAAACACGTCGCGGTAAAAAGTTAGAACATCAAGATCGTATAGATATCCCAGAATTa                                          >  2:26108/1‑149 (MQ=255)                                                                                                tAAAAACAGAAGGGATTATTGAATCTGGTGGTCAAACAAAATGGTTCTTGCAAGACGTTGAAGTATTAATTAATGGAGTGCGTGAAACACGTCGCGGTAAAAAGTTAGAACATCAAGATCGTATAGATATCCCAGAATTACCTGAAGAt                                 >  2:236407/1‑149 (MQ=255)                                                                                                   aaaCAGAAGGGATTATTGAATCTGGTGGTCAAACAAAATGGTTCTTGCAAGACGTTGAAGTATTAATTAATGGAGTGCGTGAAACACGTCGCGGTAAAAAGTTAGAACATCAAGATCGTATAGATATCCCAGAATTACCTGAAGCTGcc                              >  2:63317/1‑148 (MQ=255)                                                                                                             gATTATTGAATCTGGTGGTCAAACAAAATGGTTCTTGCAAGACGTTGAAGTATTAATTAATGGAGTGCGTGAAACACGTCGCGGTAAAAAGTTAGAACATCAAGATCGTATAGATATCCCAGAATTACCTGAAGATGCTGGttctttct                    <  1:73796/149‑1 (MQ=255)                                                                                                              attattGAATCTGGTGGTCAAACAAAATGGTTCTTGCAAGACGTTGAAGTATTAATTAATGGAGTGCGTGAAACACGTCGCGGTAAAAAGTTAGAACATCAAGATCGTATAGATATCCCAGAATTACCTGAAGATGCTGGTtctttctt                   >  2:25821/1‑149 (MQ=255)                                                                                                                    gAATCTGGTGGTCAAACAAAATGGTTCTTGCAAGACGTTGAAGTATTAATTAATGGAGTGCGTGAAACACGTCGCGGTAAAAAGTTAGAACATCAAGATCGTATAGATATCCCAGAATTACCTGAAGATGCTGGTTCTTTCTTAAtcat             >  1:110801/1‑149 (MQ=255)                                                                                                                    gAATCTGGTGGTCAAACAAAATGGTTCTTGCAAGACGTTGAAGTATTAATTAATGGAGTGCGTGAAACACGTCGCGGTAAAAAGTTAGAACATCAAGATCGTATAGATATCCCAGAATTACCTGAAGATGCTGGTTCTTTCTTAAtcat             >  2:307181/1‑149 (MQ=255)                                                                                                                       tCTGGTGGTCAAACAAAATGGTTCTTGCAAGACGTTGAAGTATTAATTAATGGAGTGCGTGAAACACGTCGCGGTAAAAAGTTAGAACATCAAGATCGTATAGATATCCCAGAATTACCTGAAGATGCTGGTTCTTTCTTAAtcattca          <  1:214778/149‑1 (MQ=255)                                                                                                                         tggtggTCAAACAAAATGGTTCTTGCAAGACGTTGAAGTATTAATTAATGGAGTGCGTGAAACACGTCGCGGTAAAAAGTTAGAACATCAAGATCGTATAGATATCCCAGAATTACCTGAAGATGCTGGTTCTTTCTTAATCATtcatc        >  1:36385/1‑149 (MQ=255)                                                                                                                          ggtggtCAAACAAAATGGTTCTTGCAAGACGTTGAAGTATTAATTAATGGAGTGCGTGAAACACGTCGCGGTAAAAAGTTAGAACATCAAGATCGTATAGATATCCCAGAATTACCTGAAGATGCTGGTTCTTTCTTAATCATtcatca       >  1:13639/1‑149 (MQ=255)                                                                                                                           gtggtCAAACAAAATGGTTCTTGCAAGACGTTGAAGTATTAATTAATGGAGTGCGTGAAACACGTCGCGGTAAAAAGTTAGAACATCAAGATCGTATAGATATCCCAGAATTACCTGAAGATGCTGGTTCTTTCTTAATCATTCATCaa      >  1:163788/1‑149 (MQ=255)                                                                                                                               tCAAACAAAATGGTTCTTGCAAGACGTTGAAGTATTAATTAATGGAGTGCGTGAAACACGTCGCGGTAAAAAGTTAGAACATCAAGATCGTATAGATATCCCAGAATTACCTGAAGATGCTGGTTCTTTCTTAATCATTCATCAAGGTg  >  2:167300/1‑149 (MQ=255)                                                                                                                                   |                                                                                                                                                 AAAGGTATAATATATTAATGACATACAAAGAAATGGAGTGATTATTTTGGTTCAAGAAGTTGTAGTAGAAGGAGACATTAATTTAGGTCAATTTCTAAAAACAGAAGGGATTATTGAATCTGGTGGTCAAGCAAAATGGTTCTTGCAAGACGTTGAAGTATTAATTAATGGAGTGCGTGAAACACGTCGCGGTAAAAAGTTAGAACATCAAGATCGTATAGATATCCCAGAATTACCTGAAGATGCTGGTTCTTTCTTAATCATTCATCAAGGTG  >  CP000730/3660‑3934

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑