Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	874,718	A→C	K541N (AAA→AAC)	clfA →	fibrinogen‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	874,718	0	A	C	100.0%	148.6 / NA	38	K541N (AAA→AAC)	clfA	fibrinogen‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (25/13);  total (25/13)

ATAGCAAAGGTGATTTAGCTTTACGTTCAACTTTATATGGGTATAACTCGAATATAATTTGGCGCTCTATGTCATGGGACAACGAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGATAAACCAGTTGTTCCTGAACAACCTGATGAGCCTGGTGAAATTGAACCAATTCCAGAGGATTCAGATTCTGACCCAGGTTCAGATTCTGGCAGCGATTCTAATTCAGATAGCGGTTCAGATTCGGGTAGTGATTCTACATCAGA  >  CP000730/874588‑874858                                                                                                                                   |                                                                                                                                             aTAGCAAAGGTGATTTAGCTTTACGTTCAACTTTATATGGGTATAACTCGAAAATACTTTGGCAAAATATGTCCTGGCACAACGAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGaacaa                                                                                                                            <  1:237749/149‑1 (MQ=255)                           tCAACTTTATATGGGTATAACTCGAATATAATTTGGCGCTCTATGTCATGGGACAACGAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGCCTGGTGAAATTGAAcc                                                                                                  >  1:306902/1‑149 (MQ=255)                                   atatGGGTATAACTCGAATATAATTTGGCGCTCTATGTCATGGGACAACGAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGCCTGGTGAAATTGAACCAATTCCag                                                                                          <  1:78740/149‑1 (MQ=255)                                     atGGGTATAACTCGAATATAATTTGGCGCTCTATGTCATGGGACAACGAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGCCTGGTGAAATTGAACCAATTCCagag                                                                                        >  2:174665/1‑149 (MQ=255)                                     atGGGTATAACTCGAATATAATTTGGCGCTCTATGTCATGGGACAACGAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGCCTGGTGAAATTGAACCAATTCCagag                                                                                        >  2:154998/1‑149 (MQ=255)                                      tGGGTATAACTCGAATATAATTTGGCGCTCTATGTCATGGGACAACGAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGCCTGGTGAAATTGAACCAATTCCAGAgg                                                                                       >  1:15545/1‑149 (MQ=255)                                             aaCTCGAATATAATTTGGCGCTCTATGTCATGGGACAACGAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGCCTGGTGAAATTGAACCAATTCCAGAGGATTCAGa                                                                                >  1:123813/1‑149 (MQ=255)                                             aaCTCGAATATAATTTGGCGCTCTATGTCATGGGACAACGAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGCCTGGTGAAATTGAACCAATTCCAGAGGATTCAGa                                                                                >  1:148685/1‑149 (MQ=255)                                                     tataATTTGGCGCTCTATGTCATGGGACAACGAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGCCTGGTGAAATTGAACCAATTCCAGAGGATTCAGATTCTGAcc                                                                        >  1:90403/1‑149 (MQ=255)                                                           ttGGCGCTCTATGTCATGGGACAACGAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGCCTGGTGAAATTGAACCAATTCCAGAGGATTCAGATTCTGACCCAGGtt                                                                  <  2:152115/149‑1 (MQ=255)                                                           ttGGCGCTCTATGTCATGGGACAACGAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGCCTGGTGAAATTGAACCAATTCCAGAGGATTCAGATTCTGACCCAGGtt                                                                  <  2:170826/149‑1 (MQ=255)                                                            tGGCGCTCTATGTCATGGGACAACGAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGCCTGGTGAAATTGAACCAATTCCAGAGGATTCAGATTCTGACCCAGGTTc                                                                 >  2:11867/1‑149 (MQ=255)                                                              gcgcTCTATGTCATGGGACAACGAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGCCTGGTGAAATTGAACCAATTCCAGAGGATTCAGATTCTGACCCAGGTTCAg                                                               <  1:29641/149‑1 (MQ=255)                                                              gcgcTCTATGTCATGGGACAACGAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGCCTGGTGAAATTGAACCAATTCCAGAGGATTCAGATTCTGACCCAGGTTCAg                                                               <  2:335622/149‑1 (MQ=255)                                                                        tCATGGGACAACGAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGCCTGGTGAAATTGAACCAATTCCAGAGGATTCAGATTCTCACCCAGGTTCAGATTCTGGCAg                                                     >  1:227335/1‑149 (MQ=255)                                                                         cATGGGACAACGAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGCCTGGTGAAATTGAACCAATTCCAGAGGATTCAGATTCTGACCCAGGTTCAGATTCTGGCAGc                                                    >  1:74466/1‑149 (MQ=255)                                                                               aCAACGAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGCCTGGTGAAATTGAACCAATTCCAGAGGATTCAGATTCTGACCCAGGTTCAGATTCTGGCAGCGATTCt                                              <  2:190436/149‑1 (MQ=255)                                                                                             tttAATAACGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGCCTGGTGAAATTGAACCAATTCCAGAGGATTCAGATTCTGACCCAGGTTCAGATTCTGGCAGCGATTCTAATTCAGATAGCgg                                >  1:216876/1‑149 (MQ=255)                                                                                               taataaCGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGCCTGGTGAAATTGAACCAATTCCAGAGGATTCAGATTCTGACCCAGGTTCAGATTCTGGCAGCGATTCTAATTCAGATAGCGGtt                              <  1:176590/149‑1 (MQ=255)                                                                                                 ataaCGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGCCTGGTGAAATTGAACCAATTCCAGAGGATTCAGATTCTGACCCAGGTTCAGATTCTGGCAGCGATTCTAATTCAGATAGCGGTTCa                            >  1:307086/1‑149 (MQ=255)                                                                                                   aaCGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGCCTGGTGAAATTGAACCAATTCCAGAGGATTCAGATTCTGACCCAGGTTCAGATTCTGGCAGCGATTCTAATTCAGATAGCGCTTCAGa                          >  2:211389/1‑149 (MQ=255)                                                                                                    aCGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGCCTGGTGAAATTGAACCAATTCCAGAGGATTCAGATTCTGACCCAGGTTCAGATTCTGGCAGCGATTCTAATTCAGATAGCGGTTCAGAt                         >  2:79859/1‑149 (MQ=255)                                                                                                    aCGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGCCTGGTGAAATTGAACCAATTCCAGAGGATTCAGATTCTGACCCAGGTTCAGATTCTGGCAGCGATTCTAATTCAGATAGCGGTTCAGAt                         >  2:97624/1‑149 (MQ=255)                                                                                                     cGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGCCTGGTGAAATTGAACCAATTCCAGAGGATTCAGATTCTGACCCAGGTTCAGATTCTGGCAGCGATTCTAATTCAGATAGCGGTTCAGAtt                        >  2:121500/1‑149 (MQ=255)                                                                                                     cGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGCCTGGTGAAATTGAACCAATTCCAGAGGATTCAGATTCTGACCCAGGTTCAGATTCTGGCAGCGATTCTAATTCAGATAGCGGTTCAGAtt                        >  2:117505/1‑149 (MQ=255)                                                                                                          cAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGCCTGGTGAAATTGAACCAATTCCAGAGGATTCAGATTCTGACCCAGGTTCAGATTCTGGCAGCGATTCTAATTCAGATAGCGGTTCAGATTCGGGt                   >  2:212834/1‑149 (MQ=255)                                                                                                          cAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGCCTGGTGAAATTGAACCAATTCCAGAGGATTCAGATTCTGACCCAGGTTCAGATTCTGGCAGCGATTCTAATTCAGATAGCGGTTCAGATTCGGGt                   <  1:243125/149‑1 (MQ=255)                                                                                                             gTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGCCTGGTGAAATTGAACCAATTCCAGAGGATTCAGATTCTGACCCAGGTTCAGATTCTGGCAGCGATTCTAATTCAGATAGCGGTTCAGATTCGGGTAGt                <  2:15545/149‑1 (MQ=255)                                                                                                                  ggTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGCCTGGTGAAATTGAACCAATTCCAGAGGATTCAGATTCTGACCCAGGTTCAGATTCTGGCAGCGATTCTAATTCAGATAGCGGTTCAGATTCGGGTAGTGATTc           >  1:130568/1‑149 (MQ=255)                                                                                                                  ggTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGCCTGGTGAAATTGAACCAATTCCAGAGGATTCAGATTCTGACCCAGGTTCAGATTCTGGCAGCGATTCTAATTCAGATAGCGGTTCAGATTCGGGTAGTGATTc           <  1:337419/149‑1 (MQ=255)                                                                                                                     gACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGCCTGGTGAAATTGAACCAATTCCAGAGGATTCAGATTCTGACCCAGGTTCAGATTCTGGCAGCGATTCTAATTCAGATAGCGGTTCAGATTCGGGTAGTGATTCTAc        >  2:226343/1‑149 (MQ=255)                                                                                                                     gACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGCCTGGTGAAATTGAACCAATTCCAGAGGATTCAGATTCTGACCCAGGTTCAGATTCTGGCAGCGATTCTAATTCAGATAGCGGTTCAGATTCGGGTAGTGATTCTAc        >  2:318793/1‑149 (MQ=255)                                                                                                                      aCGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGCCTGGTGAAATTGAACCAATTCCAGAGGATTCAGATTCTGACCCAGGTTCAGATTCTGGCAGCGATTCTAATTCAGATAGCGGTTCAGATTCGGGTAGTGATTCTACa       <  2:313321/149‑1 (MQ=255)                                                                                                                         gTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGCCTGGTGAAATTGAACCAATTCCAGAGGATTCAGATTCTGACCCAGGTTCAGATTCTGGCAGCGATTCTAATTCAGATAGCGGTTCAGATTCGGGTAGTGATTCTACATCa    >  2:315742/1‑149 (MQ=255)                                                                                                                         gTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGCCTGGTGAAATTGAACCAATTCCAGAGGATTCAGATTCTGACCCAGGTTCAGATTCTGGCAGCGATTCTAATTCAGATAGCGGTTCAGATTCGGGTAGTGATTCTACATCa    >  1:23915/1‑149 (MQ=255)                                                                                                                         gTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGCCAGGTGAAATTGAACCAATTCCAGAGGATTCAGATTCTGACCCAGGTTCAGATTCTGGCAGCGATTCTAATTCAGATAGCGGTTCAGATTCGGGTAGTGATTCTACAtaa    >  1:120068/1‑147 (MQ=255)                                                                                                                          tATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGCCTGGTGAAATTGAACCAATTCCAGAGGATTCAGATTCTGACCCAGGTTCAGATGCTGGCAGCGATTCTAATTCAGATAGCGGTTCAGATTCGGGTAGTGATTCTACATCAg   >  1:67028/1‑149 (MQ=255)                                                                                                                           aTCGATAACCCAGTTGTTCCTGAACAACCTGATGAGCCTGGTGAAATTGAACCAATTCCAGAGGATTCAGATTCTGACCCAGGTTCAGATTCTGGCAGCGATTCTAATTCAGATAGCGGTTCAGATTCGGGTAGTGATTCTACATCAGa  <  2:234254/149‑1 (MQ=255)                                                                                                                                   |                                                                                                                                             ATAGCAAAGGTGATTTAGCTTTACGTTCAACTTTATATGGGTATAACTCGAATATAATTTGGCGCTCTATGTCATGGGACAACGAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGATAAACCAGTTGTTCCTGAACAACCTGATGAGCCTGGTGAAATTGAACCAATTCCAGAGGATTCAGATTCTGACCCAGGTTCAGATTCTGGCAGCGATTCTAATTCAGATAGCGGTTCAGATTCGGGTAGTGATTCTACATCAGA  >  CP000730/874588‑874858

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑