Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	2,241,539	T→C	intergenic (‑182/+284)	murZ ← / ← fbaA	UDP‑N‑acetylglucosamine 1‑carboxyvinyltransferase/fructose‑bisphosphate aldolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,241,539	0	T	C	100.0%	87.7 / NA	24	intergenic (‑182/+284)	murZ/fbaA	UDP‑N‑acetylglucosamine 1‑carboxyvinyltransferase/fructose‑bisphosphate aldolase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/7);  total (17/7)

TTGCATTATTAGCATTATAAATTAATTTTACTTTATTTGTAAAATAGTTTATTTTAATCGTTAAAATCTTGTCTTTCTATTTTCGATTGATTAATAACAAATCATTCACCTTTACTAATATATGCTGTTCTTCCCCACTTGACCATGGTCTAAAGTCTGATTATTCAACTAAATATTGACGTTGTTTTGACAATTATTTAGTTTTAATTCTACGGTTTTCACGTAAACTTTTTTCATAAATTGCTCATTTTCCTTATAATTATATCACAATCAATTTTGATATAT  >  CP000730/2241394‑2241678                                                                                                                                                  |                                                                                                                                            ttGCATTATTAGCATTATAAATTAATTTTACTTTATTTGTAAAATAGTTTATTTTAATCGTTAAAATCTTGTCTTTCTATTTTCGATTGATTAATAACAAATCATTCACCTTTACTAATATATGCTGTTCTTCCCCACTTGACCACGGt                                                                                                                                          <  2:103058/149‑1 (MQ=255)              aTTATAAATTAATTTTACTTTATTTGTAAAATAGTTTATTTTAATCGTTAAAATCTTGTCTTTCTATTTTCGATTGATTAATAACAAATCATTCACCTTTACTAATATATGCTGTTCTTCCCCACTTGACCACGGTCTAAAGTCTGatt                                                                                                                             >  2:212846/1‑149 (MQ=255)                   aaaTTAATTTTACTTTATTTGTAAAATAGTTTATTTTAATCGTTAAAATCTTGTCTTTCTATTTTCGATTGATTAATAACAAATCATTCACCTTTACTAATATATGCTGTTCTTCCCCACTTGACCACGGTCTAAAGTCTGATTATTCa                                                                                                                        <  2:232209/149‑1 (MQ=255)                   aaaTTAATTTTACTTTATTTGTAAAATAGTTTATTTTAATCGTTAAAATCTTGTCTTTCTATTTTCGATTGATTAATAACAAATCATTCACCTTTACTAATATATGCTGTTCTTCCCCACTTGACCACGGTCTAAAGTCTGATTATTCa                                                                                                                        <  2:232103/149‑1 (MQ=255)                           tttACTTTATTTGTAAAATAGTTTATTTTAATCGTTAAAATCTTGTCTTTCTATTTTCGATTGATTAATAACAAATCATTCACCTTTACTAATATATGCTGTTCTTCCCCACTTGACCACGGTCTAAAGTCTGATTATTCAACTAAata                                                                                                                <  1:72950/149‑1 (MQ=255)                                   aTTTGTAAAATAGTTTATTTTAATCGTTAAAATCTTGTCTTTCTATCTTCGATTGATTAATAACAAATCATTCACCTTTACTAATATATGCTGTTCTTCCCCACTTGACCACGGCCTAAAGTCTGATTATTCAACTAAATATTGACgtt                                                                                                        <  2:275730/149‑1 (MQ=255)                                          aaaTAGTTTATTTTAATCGTTAAAATCTTGTCTTTCTATTTTCGATTGATTAATAACAAATCATTCACCTTTACTAATATATGCTGTTCTTCCCCACTTGACCACGGTCTAAAGTCTGATTATTCAACTAAATATTGACGTTGTTTTGa                                                                                                 >  1:218216/1‑149 (MQ=255)                                                            gTTAAAATCTTGTCTTTCTATTTTCGATTGATTAATAACAAATCATTCACCTTTACTAATATATGCTGTTCTTCCCCACTTGACCACGGTCTAAAGTCTGATTATTCAACTAAATATTGACGTTGTTTTGACAATTATTTAGTTTTAAt                                                                               >  1:264295/1‑149 (MQ=255)                                                                aaaTCTTGTCTTTCTATTTTCGATTGATTAATAACAAATCATTCACCTTTACTAATATATGCTGTTCTTCCCCACTTGACCACGGTCTAAAGTCTGATTATTCAACTAAATATTGACGTTGTTTTGACAATTATTTAGTTTTAATTCTa                                                                           >  2:62000/1‑149 (MQ=255)                                                                           ttCTATTTTCGATTGATTAATAACAAATCATTCACCTTTACTAATATATGCTGTTCTTCCCCACTTGACCACGGTCTAAAGTCTGATTATTCAACTAAATATTGACGTTGTTTTGACAATTATTTAGTTTTAATTCTACGGTTTTCACg                                                                <  2:135663/149‑1 (MQ=255)                                                                           ttCTATTTTCGATTGATTAATAACAAATCATTCACCTTTACTAATATATGCTGTTCTTCCCCACTTGACCACGGTCTAAAGTCTGATTATTCAACTAAATATTGACGTTGTTTTGACAATTATTTAGTTTTAATTCTACGGTTTTCACg                                                                >  1:131893/1‑149 (MQ=255)                                                                                                     aTCATTCACCTTTACTAATATATGCTGTTCTTCCCCACTTGACCACGGTCTAAAGTCTGATTATTCAACTAAATATTGACGTTGTTTTGACAATTATTTAGTTTTAATTCTACAGTTTTCACGTAAACTTTTTTCATAAATTGCTCAtt                                      >  2:333074/1‑149 (MQ=255)                                                                                                          tCACCTTTACTAATATATGCTGTTCTTCCCCACTTGACCACGGTCTAAAGTCTGATTATTCAACTAAATATTGACGTTGTTTTGACAATTATTTAGTTTTAATTCTACGGTTTTCACGTAAACTTTTTTCATAAATTGCTCATTTTCCt                                 <  1:170431/149‑1 (MQ=255)                                                                                                                    tAATATATGCTGTTCTTCCCCACTTGACCACGGTCTAAAGTCTGATTATTCAACTAAATATTGACGTTGTTTTGACAATTATTTAGTTTTAATTCTACGGTTTTCACGTAAACTTTTTTCATAAATTGCTCATTTTCCTTATAATtata                       >  2:107509/1‑149 (MQ=255)                                                                                                                    tAATATATGCTGTTCTTCCCCACTTGACCACGGTCTAAAGTCTGATTATTCAACTAAATATTGACGTTGTTTTGACAATTATTTAGTTTTAATTCTACGGTTTTCACGTAAACTTTTTTCATAAATTGCTCATTTTCCTTATAATtata                       >  2:54146/1‑149 (MQ=255)                                                                                                                           tGCTGTTCTTCCCCACTTGACCACGGTCTAAAGTCTGATTATTCAACTAAATATTGACGTTGTTTTGACAATTATTTAGTTTTAATTCTACGGTTTTCACGTAAACTTTTTTCATAAATTGCTCATTTTCCTTATAATTATATCAcaat                >  1:23564/1‑149 (MQ=255)                                                                                                                            gCTGTTCTTCCCCACTTGACCACGGTCTAAAGTCTGATTATTCAACTAAATATTGACGTTGTTTTGACAATTATTTAGTTTTAATTCTACGGTTTTCACGTAAACTTTTTTTTTTTTTTTCTCATTTTCCTTATAATTATATCAcaatc               >  2:131243/1‑149 (MQ=255)                                                                                                                            gCTGTTCTTCCCCACTTGACCACGGTCTAAAGTCTGATTATTCAACTAAATATTGACGTTGTTTTGACAATTATTTAGTTTTAATTCTACGGTTTTCACGTAAACTTTTTTCATAAATTGCTCATTTTCCTTATAATTATATCAcaatc               >  1:303258/1‑149 (MQ=255)                                                                                                                            gCTGTTCTTCCCCACTTGACCACGGTCTAAAGTCTGATTATTCAACTAAATATTGACGTTGTTTTGACAATTATTTAGTTTTAATTCTACGGTTTTCACGTAAACTTTTTTCATAAATTGCTCATTTTCCTTATAATTATATCAcaatc               >  2:179134/1‑149 (MQ=255)                                                                                                                             cTGTTCTTCCCCACTTGACCACGGTCTAAAGTCTGATTATTCAACTAAATATTGACGTTGTTTTGACAATTATTTAGTTTTAATTCTACGGTTTTCACGTAAACTTTTTTCATAAATTGCTCATTTTCCTTATAATTATATCAcaatca              >  1:303029/1‑149 (MQ=255)                                                                                                                             cTGTTCTTCCCCACTTGACCACGGTCTAAAGTCTGATTATTCAACTAAATATTGACGTTGTTTTGACAATTATTTAGTTTTAATTCTACGGTTTTCACGTAAACTTTTTTCATAAATTGCTCATTTTCCTTATAATTATATCAcaatca              >  1:37297/1‑149 (MQ=255)                                                                                                                              tGTTCTTCCCCACTTGACCACGGTCTAAAGTCTGATTATTCAACTAAATATTGACGTTGTTTTGACAATTATTTAGTTTTAATTCTACGGTTTTCACGTAAACTTTTTTCATAAATTGCTCATTTTCCTTATAATTATATCAcaatcaa             >  2:216657/1‑149 (MQ=255)                                                                                                                                   ttcCCCACTTGACCACGGTCTAAAGTCTGATTATTCAACTAAATATTGACGTTGTTTTGACAATTATTTAGTTTTAATTCTACGGTTTTCACGTAAACTTTTTTCATAAATTGCTCATTTTCCTTATAATTATATCACAATCAATTTTg        >  2:148284/1‑149 (MQ=255)                                                                                                                                         aCTTGACCACGGTCTAAAGTCTGATTATTCAACTAAATATTGACGTTGTTTTGACAATTATTTAGTTTTAATTCTACGGTTTTCACGTAAACTTTTTTCATAAATTGCTCATTTTCCTTATAATTATATCACAATCAATTTTGatatat  >  2:93087/1‑149 (MQ=255)                                                                                                                                                  |                                                                                                                                            TTGCATTATTAGCATTATAAATTAATTTTACTTTATTTGTAAAATAGTTTATTTTAATCGTTAAAATCTTGTCTTTCTATTTTCGATTGATTAATAACAAATCATTCACCTTTACTAATATATGCTGTTCTTCCCCACTTGACCATGGTCTAAAGTCTGATTATTCAACTAAATATTGACGTTGTTTTGACAATTATTTAGTTTTAATTCTACGGTTTTCACGTAAACTTTTTTCATAAATTGCTCATTTTCCTTATAATTATATCACAATCAATTTTGATATAT  >  CP000730/2241394‑2241678

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑