Predicted mutation
evidence seq id position mutation annotation gene description
RA CP000730 2,289,262 C→G W76C (TGG→TGC)  ybbP ← hypothetical membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,289,2620CG97.3% 135.2 / NA 37W76C (TGG→TGC) ybbPhypothetical membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base G (18/18);  minor base T (0/1);  total (18/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGATTGAATCAATTTCTCTTCATCTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATGAGAAGATAAAGT  >  CP000730/2289129‑2289406
                                                                                                                                     |                                                                                                                                                
ccGATTGAATCAATTTCTCTTCATCTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCGCATTGAATAACGata                                                                                                                                   <  2:290857/149‑1 (MQ=255)
  gATTGAATCAATTTCTCTTCATCTTTTCTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCGCATTGAATAACGATATc                                                                                                                                 <  2:269439/149‑1 (MQ=255)
                 tctTCATCTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCGCATTGAATAACGATATCGAATAATTTAGATGt                                                                                                                  <  1:135207/149‑1 (MQ=255)
                  ctTCATCTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCGCATTGAATAACGATATCGAATAATTTAGATGtt                                                                                                                 <  1:62082/149‑1 (MQ=255)
                  ctTCATCTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCGCATTGAATAACGATATCGAATAATTTAGATGtt                                                                                                                 <  2:27852/149‑1 (MQ=255)
                       tCTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCGCATTGAATAACGATATCGAATAATTTAGATGTTGCAGt                                                                                                            <  2:127187/149‑1 (MQ=255)
                         tttACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCTCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCa                                                                                                          <  1:35371/149‑1 (MQ=255)
                         tttACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCGCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCa                                                                                                          <  1:43651/149‑1 (MQ=255)
                               ataCGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCGCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCa                                                                                                    >  2:160407/1‑149 (MQ=255)
                                  cGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCGCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAata                                                                                                 >  1:50603/1‑149 (MQ=255)
                                  cGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCGCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAata                                                                                                 <  1:314230/149‑1 (MQ=255)
                                  cGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCGCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAata                                                                                                 >  1:125463/1‑149 (MQ=255)
                                   gTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCGCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAatat                                                                                                >  1:278769/1‑149 (MQ=255)
                                    tATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCGCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATc                                                                                               >  2:281883/1‑149 (MQ=255)
                                    tATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCGCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATc                                                                                               <  1:321331/149‑1 (MQ=255)
                                         gAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGACTATTACTATTAAAGCTAATACCCCGCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACt                                                                                          >  2:242054/1‑149 (MQ=255)
                                          aaGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCGCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACtt                                                                                         >  2:269671/1‑149 (MQ=255)
                                          aaGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCGCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACtt                                                                                         >  2:322627/1‑149 (MQ=255)
                                          aaGTATAGCGAAATAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCGCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACtt                                                                                         >  2:309593/1‑149 (MQ=255)
                                               taGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCGCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATctg                                                                                    >  2:61191/1‑149 (MQ=255)
                                                    ttttAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTCCTGGTTGGAATATTACTATTAAAGCTAATACCCCGCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGAc                                                                               >  1:179310/1‑149 (MQ=255)
                                                             gCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCGCATTGAATAACTATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACaa                                                                      <  2:220411/149‑1 (MQ=255)
                                                                        ccaGTTGTTCTAGCCCACGTCTAATTTCTGTTTGGAATATTACTATAAAAGCTAATACCCCGCATTGAATAACGATACCGAATAATCTAGATGTTGCAGTCAAGTTCAATATCATACTTCTCTGCTGACCAATAACAATTAATAATATc                                                           <  2:132243/147‑1 (MQ=255)
                                                                        cAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCGCATTGAATAACGATTTCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATc                                                           <  2:257305/149‑1 (MQ=255)
                                                                         aaGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCGCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATcc                                                          <  1:228368/149‑1 (MQ=255)
                                                                                     cGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATGCCCCGCGTTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAANTAAtt                                              >  1:9266/1‑149 (MQ=255)
                                                                                        aCGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCGCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTa                                           <  1:177912/149‑1 (MQ=255)
                                                                                               aTTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCGCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCttt                                    <  1:160407/149‑1 (MQ=255)
                                                                                                            atatTACTATTAAAGCTAATACCCCGCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAg                       >  1:96888/1‑149 (MQ=255)
                                                                                                              atTACTATTAAAGCTAATACCCCGCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGAc                     <  1:228726/149‑1 (MQ=255)
                                                                                                                    aTTAAAGCTAATACCCCGCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGtgat               >  1:48284/1‑149 (MQ=255)
                                                                                                                       aaaGCTAATACCCCGCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATgag            >  1:51371/1‑149 (MQ=255)
                                                                                                                       aaaGCTAATACCCCGCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATgag            >  1:237831/1‑149 (MQ=255)
                                                                                                                        aaGCTAATACCCCGCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATgaga           >  1:165497/1‑149 (MQ=255)
                                                                                                                          gCTAATACCCCGCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATGagaag         <  1:177068/149‑1 (MQ=255)
                                                                                                                          gCTAATACCCCGCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATGagaag         <  1:152487/149‑1 (MQ=255)
                                                                                                                              aTACCCCGCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATGAGAAGATaa     >  1:11227/1‑149 (MQ=255)
                                                                                                                                 ccccgcATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATGAGAAGATAAAGt  >  1:234022/1‑149 (MQ=255)
                                                                                                                                     |                                                                                                                                                
CCGATTGAATCAATTTCTCTTCATCTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATGAGAAGATAAAGT  >  CP000730/2289129‑2289406

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑