Predicted mutation
evidence seq id position mutation annotation gene description
RA CP000730 2,709,576 C→T G451S (GGT→AGT)  oatA ← possible acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,709,5760CT96.8% 100.0 / NA 31G451S (GGT→AGT) oatApossible acetyltransferase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (18/12);  total (19/12)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTGACCTTTTTTAGCATAGTCTTTATATTGCGATTTCACAATTGGTGTAGCATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGACTTTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTGTTTTAAATTCCGTCTTCTTCTCTTTCTTCTCGGCTTCATGTTGT  >  CP000730/2709432‑2709715
                                                                                                                                                |                                                                                                                                           
tttGACCTTTTTTAGCATAGTCTTTATATTGCGATTTCACAATTGGTGTAGCATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACTAAtt                                                                                                                                         <  1:214458/149‑1 (MQ=255)
        tttttAGCATAGTCTTTATATTGCGATTTCACAATTGGTGTAGCATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACTAATTAATAgtgg                                                                                                                                 >  1:247853/1‑149 (MQ=255)
                    tCTTTATATTGCGATTTCACAATTGGTGTAGCATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTATAGACATTACCAATATCCACCATGACCGAGTCACTAATTAATAGTGGTGATGACttttt                                                                                                                     >  2:22806/1‑149 (MQ=255)
                       ttATATTGCGATTTCACAATTGGTGTAGCATCAACGAGTTGCCGTCCACCTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGCGTAACTAATTAATACTGGTGATGACTTTTTAat                                                                                                                  >  2:117987/1‑149 (MQ=255)
                            ttGCGATTTCACAATTGGTGTAGCATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACTAATTAATAGTGGTGATGACTTTTTAATATCct                                                                                                             <  1:338698/149‑1 (MQ=255)
                               cggtttCACAATTGGTGTAGCATCAACGCGTTGCCGTCCAACTTTAACATCAATTTGTGCATTTGGTATTTTCTTAGTAAAAACATTATCAATATCCATCATGAACGAGATACCAATTAAAAGTTGTTATGACTTTTTatttttccttt                                                                                                          >  2:146200/4‑139 (MQ=255)
                               cGATTTCACAATTGGTGTAGCATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACTAATTAATAGTGGTGATGACTTTTTAATATCCTCtt                                                                                                          >  2:134713/1‑149 (MQ=255)
                               cGATTTCACAATTGGTGTAGCATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACTAATTAATAGTGGGGATGACTTTTTAATATACTCtt                                                                                                          >  2:199451/1‑149 (MQ=255)
                                gATTTCACAATTGGTGTAGCATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACTAATTAATAGTGGTGATGACTTTTTAATATCCTCttt                                                                                                         >  1:72560/1‑149 (MQ=255)
                                            ggTGTAGCATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACTAATTAATAGTGGTGATGACTTTTTAATATCCTCTTTGCTATTCGCTGt                                                                                             >  2:40908/1‑149 (MQ=255)
                                               gtAGCATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACTAATTAATAGTGGTGATGACTTTTTAATATCCTCTTTGCTATTCGCTGTCTg                                                                                          <  2:16725/149‑1 (MQ=255)
                                                       aaCGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACTAATTAATAGTGGTGATGACTTTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCct                                                                                  <  2:77885/149‑1 (MQ=255)
                                                                   tCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACTAATTAATAGTGGTGATGACTTTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCtt                                                                      >  1:10157/1‑149 (MQ=255)
                                                                           tACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACTAATTAATAGTGGTGATGACTTTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACg                                                              >  1:313759/1‑149 (MQ=255)
                                                                           tACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACTAATTAATAGTGGTGATGACTTTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACg                                                              <  2:12209/149‑1 (MQ=255)
                                                                                 cAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACTAATTAATAGTGGTGATGACTTTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACtttt                                                        >  1:339228/1‑149 (MQ=255)
                                                                                                  aTTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACTAATTAATAGTGGTGATGACTTTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTGTTTTaaa                                       >  2:224679/1‑149 (MQ=255)
                                                                                                           gTAAAGACATTACCAATATCGACCATGATCGAGTAACTAATTAATAGTGGTGATGACTTTTTAATAGTCTCTTTGCTATTCGCTGTCTGATTATCCGCTTGCTTATCTTTTTTATTGACTTTTTTTTTCGTTGTTTTATATTccttttt                              >  2:218274/1‑144 (MQ=255)
                                                                                                            tAAAGACATTACCAATATCCACCATGACCGAGTCACTAATTAATAGTGGTGATGACTTTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTGTTTTAAATTCCGtcttc                             >  2:294590/1‑149 (MQ=255)
                                                                                                             aaaGACATTACCAATATCCACCATGACCGAGTCACTAATTAATAGTGGTGATGACTTTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTGTTTTAAATTCCGtcttct                            <  1:35244/149‑1 (MQ=255)
                                                                                                              aaGACATTACCAATATCCACCATGACCGAGTCACTAATTAATAGTGGTGATGACTTTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTGTTTTAAATTCCGtcttctt                           >  2:188404/1‑149 (MQ=255)
                                                                                                               aGACATTACCAATATCCACCATGACCGAGTCACTAATTAATAGTGGTGATGACTTTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTGTTTTAAATTCCGtcttcttc                          <  1:289814/149‑1 (MQ=255)
                                                                                                                gACATTACCAATATCCACCATGACCGAGTCACTAATTAATAGTGGTGATGACTTTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTGTTTTAAATTCCGtcttcttct                         >  1:91771/1‑149 (MQ=255)
                                                                                                                 aCATTACCAATATCCACCATGACCGAGTCACTAATTAATAGTGGTGATGACTTTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTGTTTTAAATTCCGTCTTCTtctc                        >  2:74709/1‑149 (MQ=255)
                                                                                                                    ttACCAATATCCACCATGACCGAGTCACTAATTAATAGTGGTGATGACTTTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTGTTTTAAATTCCGTCTTCTTCtcttt                     <  2:99159/149‑1 (MQ=255)
                                                                                                                         aaTATCCACCATGACCGAGTCACTAATTAATAGTGGTGATGACTTTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTGTTTTAAATTCCGTCTTCTTCTCTTtcttct                >  2:59415/1‑149 (MQ=255)
                                                                                                                             tCCACCATGACCGAGTCACTAATTAATAGTGGTGATGACTTTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTGTTTTAAATTCCGTCTTCTTCTCTTTCTTCTCGGc            <  1:134713/149‑1 (MQ=255)
                                                                                                                                 ccaTGACCGAGTCACTAATTAATAGTGGTGATGACTTTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTGTTTTAAATTCCGTCTTCTTCTCTTTCTTCTCGGCTTCa        <  2:10157/149‑1 (MQ=255)
                                                                                                                                      aCCGAGTCACTAATTAATAGTGGTGATGACTTTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTGTTTTAAATTCCGTCTTCTTCTCTTTCTTCTCGGCTTCAtgttg   >  1:162723/1‑149 (MQ=255)
                                                                                                                                       ccGAGTCACTAATTAATAGTGGTGATGACTTTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTGTTTTAAATTCCGTCTTCTTCTCTTTCTTCTCGGCTTCAtgttgt  <  1:9744/149‑1 (MQ=255)
                                                                                                                                       ccGAGTCACTAATTAATAGTGGTGATGACTTTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTGTTTTAAATTCCGTCTTCTTCTCTTTCTTCTCGGCTTCAtgttgt  <  2:10877/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                           
TTTGACCTTTTTTAGCATAGTCTTTATATTGCGATTTCACAATTGGTGTAGCATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGACTTTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTGTTTTAAATTCCGTCTTCTTCTCTTTCTTCTCGGCTTCATGTTGT  >  CP000730/2709432‑2709715

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑