| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP000730 | 722,155 | A→T | 11.1% | K42N (AAA→AAT) | fhuC → | iron (Fe3+) ABC superfamily ATP binding cassette transporter, ABC protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 722,155 | 0 | A | T | 11.1% | 101.6 / 7.2 | 36 | K42N (AAA→AAT) | fhuC | iron (Fe3+) ABC superfamily ATP binding cassette transporter, ABC protein |
| Reads supporting (aligned to +/- strand): ref base A (14/18); new base T (2/2); total (16/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.41e-01 | |||||||||||
TTTTATTGAAAGTAGGGAAATTATGAATCGTTTGCATGGACAACAAGTTAAAATTGGTTACGGGGATAACACGATTATAAATAAATTAGATGTTGAAATACCAGATGGCAAAGTGACGTCAATCATTGGTCCTAACGGCTGCGGGAAATCTACTTTGCTAAAGGCATTGTCACGTTTATTGGCAGTTAAAGAAGGCGAAGTATTTTTAGATGGTGAAAATATTCATACACAATCTACGAAAGAGATTGCAAAAAAAATAGCCATTTTACCTCAATCACCTGAAGTAGCAGATGGC > CP000730/722008‑722302 | ttttATTGAAAGTAGGGAAATTATGAATCGTTTGCATGGACAACAAGTTAAAATTGGTTACGGGGATAACACGATTATAAATAAATTAGATGTTGAAATACCAGATGGCAAAGTGACGTCAATCATTGGTCCTAACGGCTGCGGGAAAt < 2:190318/149‑1 (MQ=255) tttATTGAAAGTAGGGAAATTATGAATCGTTTGCATGGACAACAAGTTAAAATTGGTTACGGGGATAACACGATTATAAATAAATTAGATGTTGAAATACCAGATGGCAAAGTGACGTCAATCATTGGTCCTAACGGCTGCGGGAAATc > 1:167736/1‑149 (MQ=255) ttATTGAAAGTAGGGAAATTATGAATCGTTTGCATGGACAACAAGTTAAAATTGGTTACGGGGATAACACGATTATAAATAAATTAGATGTTGAAATACCAGATGGCAAAGTGACGTCAATCATTGGTCCTAACGGCTGCGGGAAATCt < 1:267134/149‑1 (MQ=255) tGAAAGTAGGGAAATTATGAATCGTTTGCATGGACAACAAGTTAAAATTGGTTACGGGGATAACACGATTATAAATAAATTAGATGTTGAAATACCAGATGGCAAAGTGACGTCAATCATTGGTCCTAACGGCTGCGGGAAATCTACtt < 2:92190/149‑1 (MQ=255) aGGTAAATTATGAATCGTTTGCATGGACAACAAGTTAAAATTGGTTACGGGGATAACACGATTATAAATAAATTAGATGTTGAAATACCAGATGGCAAAGTGACGTCAATCATTGGTCCTAACGGCTGCGGGAAATCTACTTTGCTaaa < 1:152947/149‑1 (MQ=255) ggAAATTATGAATCGTTTGCATGGACAACAAGTTAAAATTGGTTACGGGGATAACACGATTATAAATAAATTAGATGTTGAAATACCAGATGGCAAAGTGACGTCAATCATTGGTCCTAACGGCTGCGGGAATTCTACTTTGCTAAAgg > 1:130833/1‑149 (MQ=255) ggAAATTATGAATCGTTTGCATGGACAACAAGTTAAAATTGGTTACGGGGATAACACGATTATAAATAAATTAGATGTTGAAATACCAGATGGCAAAGTGACGTCAATCATTGGTCCTAACGGCTGCGGGAATTCTACTTTGCTAAAgg > 1:130837/1‑149 (MQ=255) ggAAATTATGAATCGTTTGCATGGACAACAAGTTAAAATTGGTTACGGGGATAACACGATTATAAATAAATTAGATGTTGAAATACCAGATGGCAAAGTGACGTCAATCATTGGTCCTAACGGCTGCGGGAAATCTACTTTGCTAAAgg > 2:146629/1‑149 (MQ=255) ggAAATTATGAATCGTTTGCATGGACAACAAGTTAAAATTGGTTACGGGGATAACACGATTATAAATAAATTAGATGTTGAAATACCAGATGGCAAAGTGACGTCAATCATTGGTCCTAACGGCTGCGGGAAATCTACTTTGCTAAAgg < 2:111728/149‑1 (MQ=255) ggAAATTATGAATCGTTTGCATGGACAACAAGTTAAAATTGGTTACGGGGATAACACGATTATAAATAAATTAGATGTTGAAATACCAGATGGCAAAGTGACGTCAATCATTGGTCCTAACGGCTGCGGGAAATCTACTTTGCTAAAgg > 1:210713/1‑149 (MQ=255) tATGAATCGTTTGCATGGACAACAAGTTAAAATTGGTTACGGGGATAACACGATTATAAATAAATTAGATGTTGAAATACCAGATGGCAAAGTGACGTCAATCATTGGTCCTAACGGCTGCGGGAAATCTACTTTGCTAAAGGCATTGt > 1:167208/1‑149 (MQ=255) aTCGTTTGCATGGACAACAAGTTAAAATTGGTTACGGGGATAACACGATTATAAATAAATTAGATGTTGAAATACCAGATGGCAAAGAGACGTCAATCATTGGTCCTAACGGCTGCGGGAAATCTACTTTGCTAAAGGCATTGTCACGt > 1:90754/1‑149 (MQ=255) tGGACAACAAGTTAAAATTGGTTACGGGGATAACACGATTATAAATAAATTAGATGTTGAAATACCAGATGGCAAAGTGACGTCAATCATTGGTCCTAACGGCTGCGGGAAATCTACTTTGCTAAAGGCATTGTCACGTTTATTGGCAg < 1:48174/149‑1 (MQ=255) tGGACAACAAGTTAAAATTGGTTACGGGGATAACACGATTATAAATAAATTAGATGTTGAAATACCAGATGGCAAAGTGACGTCAATCATTGGTCCTAACGGCTGCGGGAAATCTACTTTGCTAAAGGCATTGTCACGTTTATTGGCAg < 1:54557/149‑1 (MQ=255) ggACAACAAGTTAAAATTGGTTACGGGGATAACACGATTATAAATAAATTAGATGTTGAAATACCAGATGGCAAAGTGACGTCAATCATTGGTCCTAACGGCTGCGGGAAATCTACTTTGCTAAAGGCATTGTCACGTTTATTGGCAGt < 2:147792/149‑1 (MQ=255) gACAACAAGTTAAAATTGGTTACGGGGATAACACGATTATAAATAAATTAGATGTTGAAATACCAGATGGCAAAGTGACGTCAATCATTGGTCCTAACGGCTGCGGGAAATCTACTTTGCTAAAGGCATTGTCACGTTTATTGGCAGtt > 2:222737/1‑149 (MQ=255) aaTTGGTTACGGGGATAACACGATTATAAATAAATTAGATGTTGAAATACCAGATGGCAAAGTGACGTCAATCATTGGTCCTAACGGCTGCGGGAAATCTACTTTGCTAAAGGCATTGTCACGTTTATTGGCAGTTAAAGAAGGCGAAg > 2:111081/1‑149 (MQ=255) ttATAAATAAATTAGATGTTGAAATACCAGATGGCAAAGTGACGTCAATCATTGGTCCTAACGGCTGCGGGAAATCTACTTTGCTAAAGGCATTGTCACGTTTATTGGCAGTTAAAGAAGGCGAAGTATTTTTAGATGGTGAAAATAtt < 1:143876/149‑1 (MQ=255) aaataaatTAGATGTTGAAATACCAGATGGCAAAGTGACGTCAATCATTGGTCCTAACGGCTGCGGGAAATCTACTTTGCTAAAGGCATTGTCACGTTTATTGGCAGTTAAAGAAGGCGAAGTATTTTTAGATGGTGAAAATATTCATa < 2:202003/149‑1 (MQ=255) taaatTAGATGTTGAAATACCAGATGGCAAAGTGACGTCAATCATTGGTCCTAACGGCTGCGGGAAATCTACTTTGCTAAAGGCATTGTCACGTTTATTGGCAGTTAAAGAAGGCGAAGTATTTTTAGATGGTGAAAATATTCATacac > 2:42765/1‑149 (MQ=255) aaatGAGATGTTGAAATACCAGATGGCAAAGTGACGTCAATCATTGGTCCTACCGGCGGCGGGAAATCTACTTTGCTAAAGGCATTGTCACGTTTATTGGCAGTTAAAGAAGGCGAAGTATTTTTAGATGGTGAAAATATTCATAcaca < 1:48786/149‑1 (MQ=255) aaTACCAGATGGCAAAGTGACGTCAATCATTGGTCCTAACGGCTGCGGGAAATCTACTTTGCTAAAGGCATTGTCACGTTTATTGGCAGTTAAAGAAGGCGAAGTATTTTTAGATGGTGAAAATATTCATACACAATCTACGAAAgaga > 2:261074/1‑149 (MQ=255) aaTACCAGATGGCAAAGTGACGTCAATCATTGGTCCTAACGGCTGCGGGAAATCTACTTTGCTAAAGGCATTGTCACGTTTATTGGCAGTTAAAGAAGGCGAAGTATTTTTAGATGGTGAAAATATTCATACACAATCTACGAAAgaga > 1:129255/1‑149 (MQ=255) aCCAGATGGCAAAGTGACGTCAATCATTGGTCCTAACGGCTGCGGGAAATCTACTTTGCTAAAGGCATTGTCACGTTTATTGGCAGTTAAAGAAGGCGAAGTATTTTTAGATGGTGAAAATATTCATACACAATCTACGAAAGAGATTg < 2:136461/149‑1 (MQ=255) gATGGCAAAGTGACGTCAATCATTGGTCCTAACGGCTGCGGGAAATCTACTTTGCTAAAGGCATTGTCACGTTTATTGGCAGTTAAAGAAGGCGAAGTATTTTTAGATGGTGAAAATATTCATACACAATCTACGAAAGAGATTGCaaa > 2:151977/1‑149 (MQ=255) aaaGTGACGTCAATCATTGGTCCTAACGGCTGCGGGAAATCTACTTTGCTAAAGGCATTGTCACGTTTATTGGCAGTTAAAGAAGGCGAAGTATTTTTAGATGGTGAAAATATTCATACACAATCTACGAAAGAGATTGCAAAAAAAAt < 1:84008/149‑1 (MQ=255) aTCATTTGTCCTAACGGCTGCGGGAAAGCTACTTTGCTAAAGGCATTGTCACGTTTATTGGCAGTTAAAGAAGGCGAAGTATTTTTAGATGGTGAAAATATTCATACACAATCTACGAAAGAGATTGCAAAAAAAATAGCCATTTTAcc < 2:8330/149‑1 (MQ=255) aTCATTGGTCCTAACGGCTGCGGGAAATCTACTTTGCTAAAGGCATTGTCACGTTTATTGGCAGTTAAAGAAGGCGAAGTATTTTTAGATGGTGAAAATATTCATACACAATCTACGAAAGAGATTGCAAAAAAAATAGCCATTTTAcc > 1:123736/1‑149 (MQ=255) tCCTAACGGCTGCGGGAAATCTACTTTGCTAAAGGCATTGTCACGTTTATTGGCAGTTAAAGAAGGCGAAGTATTTTTAGATGGTGAAAATATTCATACACAATCTACGAAAGAGATTGCAAAAAAAATAGCCATTTTACCTCAATCAc > 1:78595/1‑149 (MQ=255) aaCGGCTGCGGGAAATCTACTTTGCTAAAGGCATTGTCACGTTTATTGGCAGTTAAAGAAGGCGAAGTATTTTTAGATGGTGAAAATATTCATACACAATCTACGAAAGAGATTGCAAAAAAAATAGCCATTTTACCTCAATCACCTGa < 1:78015/149‑1 (MQ=255) aCGGCTGCGGGAAATCTACTTTGCTAAAGGCATTGTCACGTTTATTGGCAGTTAAAGAAGGCGAAGTATTTTTAGATGGTGAAAATATTCATACACAATCTACGAAAGAGATTGCAAAAAAAATAGCCATTTTACCTCAATCACCTGaa < 2:256839/149‑1 (MQ=255) ggCTGCGGGAATTCTACTTTGCTAAAGGCATTGTCACGTTTATTGGCAGTTAAAGAAGGCGAAGTATTTTTAGATGGTGAAAATATTCATACACAATCTACGAAAGAGATTGCAAAAAAAATAGCCATTTTACCTCAATCACCTGAAGt < 2:130833/149‑1 (MQ=255) ggCTGCGGGAATTCTACTTTGCTAAAGGCATTGTCACGTTTATTGGAAGTTAAAGAAGGCGAAGTATTTTTAGATGGTGAAAATATTCATACACAATCTACGAAAAAAAAAAAAAAAAAAATAGCCATTTTACCTCAATCACCTGAAGt < 2:130837/149‑1 (MQ=255) ggCTGCGGGAAATCTACTTTGCTAAAGGCATTGTCACGTTTATTGGCAGTTAAAGAAGGCGAAGTATTTTTAGATGGTGAAAATATTCATACACAATCTACGAAAGAGATTGAAAAAAAAATAGCCATTTTACCTCAATCACCTGAAGt < 2:210713/149‑1 (MQ=255) ggCTGCGGGAAATCTACTTTGCTAAAGGCATTGTCACGGGTAGTGGCAGTTAAAGAAGGCGAAGTATTTTTAGATGGTGAAAATATTCATACACAATATAAGAAAAAAAAAAAAAAAAAAATAGCCATTTTACCTCAATCACCTGAAGt < 1:146629/149‑1 (MQ=255) gggAAATCTACTTTGCTAAAGGCATTGTCACGTTTATTGGCAGTTAAAGAAGGCGAAGTATTTTTAGATGGTGAAAATATTCATACACAATCTACGAAAGAGATTGCAAAAAAAATAGCCATTTTACCTCAATCACCTGAAGTAGCAGa > 2:47082/1‑149 (MQ=255) aaTCTACTTTGCTAAAGGCATTGTCACGTTTATTGGCAGTTAAAGAAGGCGAAGTATTTTTAGATGGTGAAAATATTCATACACAATCTACGAAAGAAATAAAAAAAAAAATAGCCATTTTACCTCAATCACCTGAAGTAGCAGATGGc < 2:141513/149‑1 (MQ=255) | TTTTATTGAAAGTAGGGAAATTATGAATCGTTTGCATGGACAACAAGTTAAAATTGGTTACGGGGATAACACGATTATAAATAAATTAGATGTTGAAATACCAGATGGCAAAGTGACGTCAATCATTGGTCCTAACGGCTGCGGGAAATCTACTTTGCTAAAGGCATTGTCACGTTTATTGGCAGTTAAAGAAGGCGAAGTATTTTTAGATGGTGAAAATATTCATACACAATCTACGAAAGAGATTGCAAAAAAAATAGCCATTTTACCTCAATCACCTGAAGTAGCAGATGGC > CP000730/722008‑722302 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |