| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP000730 | 796,114 | C→A | 22.6% | S345Y (TCT→TAT) | recQ1 → | ATP‑dependent helicase RecQ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 796,114 | 0 | C | A | 22.6% | 36.1 / 4.3 | 17 | S345Y (TCT→TAT) | recQ1 | ATP‑dependent helicase RecQ |
| Reads supporting (aligned to +/- strand): ref base C (9/4); new base A (2/2); total (11/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.84e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGCTTTGTTATTCATTATAATATGCCTGGAGATTTAGAATCTTATTATCAAGAAGCGGGTCGTGCAGGTCGTGACGGGTTGAAAAGTGAATGTATTTTGTTATTTAGCGAACGCGATATCAATTTACACGAGTATTTTATAACAGTCTCTCAAGCTGATGATGACTATAAAGATAAAATGGGCGAAAAGTTAACTAAAATGATTCAATATACAAAAACAAAAAAATGTCTAGAAGCAACAATTGTCCATTATTTTGAACCGAATGAAAAATTAGAAGAATGTGAACAATGTAGT > CP000730/795966‑796259 | cGCTTTGTTATTCATTATAATATGCCTGGAGATTTAGAATCTTATTATCAAGAAGCGGGTCGTGCAGGTCGTGACGGGTTGAAAAGTGAATGTATTTTGTTATTTAGCGAACGCGATATCAATTTACACGAGTATTTTATAACAGtctc > 1:100818/1‑149 (MQ=255) tcTTTGTGATTCAGTATAATATGCCTGGAGATTTAGAATCTTATTATCAAGAATCGGGTCGTGCATGTCGTGACGGGTTGAAAAGTGAATGTATTTTATTATTTAAAGGACGTGATATCAAGTTACACGAGTATTTTATAACAGtctct < 2:241073/148‑1 (MQ=255) ttCATTANAATATGCCTGGAGATTTAGAATCTTATTATCAAGAAGCGGGTCGTGTAGGTCGTGACGGGTTGAAAAGTGAATGTATTTTGTTATTTAGCGAACGCGATATCAATTTACACGAGTATTTTATAACAGTCTCTCAAGCtgat < 2:226296/149‑1 (MQ=255) taataTGCCTGGAGATTTAGAATCTTATTATCAAGAAGCGGGTCGTGCAGGTCGTGACGGGTTGAAAAGTGAATGTATTTTGTTATTTAGCGAACGCGATATCAATTTACACGAGTATTTTATAACAGTCTCTCAAGCTGATGATGACt < 2:292448/149‑1 (MQ=255) aataTGCCTGGAGATTTAGAATCTTATTATCAAGAAGCGGGTCGTGCAGGTCGTGACGGGTTGAAAAGTGAATGTATTTTGTTATTTAGCGAACGCGATATCAATTTACACGAGTATTTTATAACAGTCTATCAAGCTGATGATGACta > 2:17604/1‑149 (MQ=255) aataTGCCTGGAGATTTAGAATCTTATTATCAAGAAGCGGGTCGTGCAGGTCGTGACGGGTTGAAAAGTGAATGTATTTTGTTATTTAGCGAACGCGATATCAATTTACACGAGTATTTTATAACAGTCTATCAAGCTGATGATGACta > 2:17690/1‑149 (MQ=255) tatGCCTGGAGATTTAGAATCTTATTATCAAGAAGCGGGTCGTGCAGGTCGTGACGGGTTGAAAAGTGAATGTATTTTGTTATTTAGCGAACGCGATATCAATTTACACGAGTATTTTATAACAGTCTCTCAAGCTGATGATGACtata > 2:237885/1‑149 (MQ=255) atGCCTGGAGATTTAGAATCTTATTATCAAGAAGCGGGTCGTGCAGGTCGTGACGGGTTGAAAAGTGAATGTATTTTGTTATTTAGCGAACGCGATATCAATTTACACGAGTATTTTATAACAGTCTCTCAAGCTGATGATGACTATaa < 1:258525/149‑1 (MQ=255) aaTCTTATTATCAAGAAGCGGGTCGTGCAGGTCGTGACGGGTTGAAAAGTGAATGTATTTTGTTATTTAGCGAACGCGATATCAATTTACACGAGTATTTTATAACAGTCTCTCAAGCTGATGATGACTATAAAGATAAAATGGGCGaa > 1:278084/1‑149 (MQ=255) cTTATTATCAAGAAGCGGGTCGTGCAGGTCGTGACGGGTTGAAAAGTGAATGTATTTTGTTATTTAGCGAACGCGATATCAATTGACACTAGTATTTGATAACAGTCTCTCAAGCTTATGATGACTATAAAGATAAAATGGGCGAAAAg > 1:301902/1‑149 (MQ=255) atCAAGAAGCGGGTCGTGCAGGTCGTGACGGGTTGAAAAGTGAATGTATTTTGTTATTTAGCGAACGCGATATCAATTTACACGAGTATTTTATAACAGTCTCTCAAGCTGATGATGACTATAAAGATAAAATGGGCGAAAAGTTAACt > 2:114447/1‑149 (MQ=255) gTGCAGGTCGTGACGGGTTGAAAAGTGAATGTATTTTGTTATTTAGCGAACGCGATATCAATTTACACGAGTATTTTATAACAGTCTCTCAAGCTGATGATGACTATACAGATAAAATGGGCGAAAAGTTAACTAAAATGATTCAatat < 2:107354/149‑1 (MQ=255) cAGGTCGTGACGGGTTGAAAAGTGAATGTATTTTGTTATTTAGCGAACGCGATATCAATTTACACGAGTATTTTATAACAGTCTCTCAAGCTGATGATGACTATAAAGATAAAATGGGCGAAAAGTTAACTAAAATGATTCAATATaca > 1:14535/1‑149 (MQ=255) gggTTGAAAAGTGAATGTATTTTGTTATTTAGCGAACGCGATATCAATTTACACGAGTATTTTATAACAGTCTCTCAAGCTGATGATGACTATAAAGATAAAATGGGCGAAAAGTTAACTAAAATGATTCAATATACAAAAACaaaaaa > 2:169124/1‑149 (MQ=255) ttttGTTATTTAGCGAACGCGATATCAATTTACACGAGTATTTTATAACAGTCTCTCAAGCTGATGATGACTATAAAGATAAAATGGGCGAAAAGTTAACTAAAATGATTCAATATACAAAAACAAAAAAATGTCTAGAAGCAACAAtt > 2:181593/1‑149 (MQ=255) cGAACGCGATATCAATTTACACGAGTATTTTATAACAGTCTCTCAAGCTGATGATGACTATAAAGATAAAATGGGCGAAAAGTTAACTAAAATGATTCAATATACAAAAACAAAAAAATGTCTAGAAGCAACAATTGTCCATTATTTTg > 1:262483/1‑149 (MQ=255) cGAGTATTTTATAACAGTCTCTCAAGCTGATGATGACTATAAAGATAAAATGGGCGAAAAGTTAACTAAAATGATTCAATATACAAAAACAAAAAAATGTCTAGAAGCAACAATTGTCCATTATTTTGAACCGAATGAAAAATTagaag > 1:215651/1‑149 (MQ=255) gTATTTTATAACAGTCTATCAAGCTGATGATGACTATAAAGATAAAATGGGCGAAAAGTTAACTAAAATGATTCAATATACAAAAACAAAAAAATGTCTAGAAGCAACAATTGTCCATTATTTTGAACCGAATGAAAAATTAGAAGAAt < 1:17690/149‑1 (MQ=255) gTATTTTATAACAGTCTATCAAGCTGATGATGACTATAAAGATAAAATGGGCGAAAAGTTAACTAAAATGATTCAATATACAAAAACAAAAAAATGTCTAGAAGCAACAATTGTCCATTATTTTGAACCGAATGAAAAATTAGAAGAAt < 1:17604/149‑1 (MQ=255) tctctcAAGCTGATGATGACTATAAAGATAAAATGGGCGAAAAGTTAACTAAAATGATTCAATATACAAAAACAAAAAAATGTCTAGAAGCAACAATTGTCCATTATTTTGAACCGAATGAAAAATTAGAAGAATGTGAACAATgtagt > 1:25541/1‑149 (MQ=255) | CGCTTTGTTATTCATTATAATATGCCTGGAGATTTAGAATCTTATTATCAAGAAGCGGGTCGTGCAGGTCGTGACGGGTTGAAAAGTGAATGTATTTTGTTATTTAGCGAACGCGATATCAATTTACACGAGTATTTTATAACAGTCTCTCAAGCTGATGATGACTATAAAGATAAAATGGGCGAAAAGTTAACTAAAATGATTCAATATACAAAAACAAAAAAATGTCTAGAAGCAACAATTGTCCATTATTTTGAACCGAATGAAAAATTAGAAGAATGTGAACAATGTAGT > CP000730/795966‑796259 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |