Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	1,292,117	G→A	13.3%	E597K (GAA→AAA)	infB →	translation initiation factor IF‑2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,292,117	0	G	A	13.3%	83.7 / 4.8	30	E597K (GAA→AAA)	infB	translation initiation factor IF‑2
Reads supporting (aligned to +/- strand):  ref base G (15/11);  new base A (2/2);  total (17/13)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

ACGTGACACTTGCTAATGCCTCAAATGGTATTATCATTGGTTTCAATGTTCGTCCAGACAGTGGTGCAAAACGTGCTGCAGAAGCTGAAAATGTTGATATGCGTTTACACAGAGTTATTTATAATGTTATCGAAGAAATTGAATCAGCGATGAAAGGTTTACTTGATCCAGAATTTGAAGAACAAGTTATCGGACAAGCTGAAGTTCGTCAAACATTCAAAGTTTCTAAAGTTGGTACTATTGCTGGATGTTATGTTACTGAAGGTAAAATTACGCGAAATGCTGGTG  >  CP000730/1291977‑1292264                                                                                                                                             |                                                                                                                                                    aCGTGACACTTGCTAATGCCTCAAATGGTATTATCATTGGTTTCAATGTTCGTCCAGACAGTGGTGCAAAACGTGCTGCAGAAGCTGAAAATGTTGATATGCGTTTACACAGAGTTATTTATAATGTTATCGAAGAAATTGAATCAGCg                                                                                                                                             >  2:73487/1‑149 (MQ=255) aCGTGACACTTGCTAATGCCTCAAATGGTATTATCATTGGTTTCAATGTTCGTCCAGACAGTGGTGCAAAACGTGCTGCAGAAGCTGAAAATGTTGATATGCGTTTACACAGAGTTATTTATAATGTTATCGAAGAAATTGAATCAGCg                                                                                                                                             >  2:150075/1‑149 (MQ=255) aCGTGACACTTGCTAATGCCTCAAATGGTATTATCATTGGTTTCAATGTTCGTCCAGACAGTGGTGCAAAACGTGCTGCAGAAGCTGAAAATGTTGATATGCGTTTACACAGAGTTATTTATAATGTTATCGAAGAAATTAAATCAGCg                                                                                                                                             >  2:198805/1‑149 (MQ=255) aCGTGACACTTGCTAATGCCTCAAATGGTATTATCATTGGTTTCAATGTTCGTCCAGACAGTGGTGCAAAACGTGCTGCAGAAGCTGAAAATGTTGATATGCGTTTACACAGAGTTATTTATAATGTTATCGAAGAAATTAAATCAGCg                                                                                                                                             >  2:38810/1‑149 (MQ=255)  cGTGACACTTGCTAATGCCTCAAATGGTATTATCATTGGTTTCAATGTTCGTCCAGACAGTGGTGCAAAACGTGCTGCAGAAGCTGAAAATGTTGATATGCGTTTACACAGAGTTATTTATAATGTTATCGAAGAAATTGAATCAGCGa                                                                                                                                            <  1:78736/149‑1 (MQ=255)                   ccTCAAATGGTATTATCATTGGTTTCAATGTTCGTCCAGACAGTGGTGCAAAACGTGCTGCAGAAGCTGAAAATGTTGATATGCGTTTACACAGAGTTATTTATAATGTTATCGAAGAAATTGAATCAGCGATGAAAGGTTTACTTGAt                                                                                                                           >  2:241611/1‑149 (MQ=255)                                  tCATTGGTTTCAATGTTCGTCCAGACAGTGGTGCAAAACGTGCTGCAGAAGCTGAAAATGTTGATATGCGTTGACACAGATCTTGTTATAATGTAATCGAAGAAATTGAATCAGCGATGAAAGGTTTACTTGATCCAGAATTTgaagaa                                                                                                            >  1:255793/1‑149 (MQ=255)                                        gTTTCAATGTTCGTCCAGACAGTGGTGCAAAACGTGCTGCAGAAGCTGAAAATGTTGATATGCGTTTACACAGAGTTATGTATAATGTTATCGAAGAAATTGAATCAGCGATGAAAGGTTTACTTGATCCAGAATTTGAAGAACAAGtt                                                                                                      >  1:301978/1‑149 (MQ=255)                                                gTTCGACCAGACAATGGTCCACAACGCGCTGCAGAAGCTGAAAATGTTCACATGCGTTTACACAGAGCTATTTATAATGTTGTCGAAGAAATTGAATCAGCGACGAAAGGTTTACTTGATCCAGAATTTGAAGAACAAGTTATCGGACa                                                                                              >  2:271958/1‑149 (MQ=255)                                                                gtgCAAAACGTGCTGCAGAAGCTGAAAATGTTGATATGCGTTTACACAGAGTTATTTATAATGTTATCGAAGAAATTGAATCAGCGATGAAAGGTTTACTTGATCCAGAATTTGAAGAACAAGTTATCGGACAAGCTGAAGTTCGTCaa                                                                              <  1:99503/149‑1 (MQ=255)                                                                 tgCAAAACGTGCTGCAGAAGCTGAAAATGTTGATATGCGTTTACACAGAGTTATTTATAATGTTATCGAAGAAATTGAATCAGCGATGAAAGGTTTACTTGATCCAGAATTTGAAGAACAAGTTATCGGACAAGCTGAAGTTCGTCaaa                                                                             >  1:147055/1‑149 (MQ=255)                                                                 tgCAAAACGTGCTGCAGAAGCTGAAAATGTTGATATGCGTTTACACAGAGTTATTTATAATGTTATCGAAGAAATTGAATCAGCGATGAAAGGTTTACTTGATCCAGAATTTGAAGAACAAGTTATCGGACAAGCTGAAGTTCGTCaaa                                                                             <  1:31193/149‑1 (MQ=255)                                                                             tgcAGAAGCTGAAAATGTTGATATGCGTTTACACAGAGTTATTTATAATGTTATCGAAGAAATTGAATCAGCGATGAAAGGTTTACTTGATCCAGAATTTGAAGAACAAGTTATCGGACAAGCTGAAGTTCGTCAAACATTCAAAGttt                                                                 <  2:265439/149‑1 (MQ=255)                                                                                       gAAAATGTTGATATGCGTTTACACAGAGTTATTTATAATGTTATCGAAGAAATTGAATCAGCGATGAAAGGTTTACTTGATCCAGAATTTGAAGAACAAGTTATCGGACAAGCTGAAGTTCGTCAAACATTCAAAGTTTCTAAAGTTgg                                                       <  1:187189/149‑1 (MQ=255)                                                                                       gAAAATGTTGATATGCGTTTACACAGAGTTATTTATAATGTTATCGAAGAAATTGAATCAGCGATGAAAGGTTTACTTGATCCAGAATTTGAAGAACAAGTTATCGGACAAGCTGAAGTTCGTCAAACATTCAAAGTTTCTAAAGTTgg                                                       <  1:213411/149‑1 (MQ=255)                                                                                       gAAAATGTTGATATGCGTTTACACAGAGTTATTTATAATGTTATCGAAGAAATTAAATCAGCGATGAAAGGTTTACTTGATCCAGAATTTGAAGAACAAGTTATCGGACAAGCTGAAGTTCGTCAAACATTCAAAGTTTCTAAAGTTgg                                                       <  1:198805/149‑1 (MQ=255)                                                                                       gAAAATGTTGATATGCGTTTACACAGAGTTATTTATAATGTTATCGAAGAAATTAAATCAGCGATGAAAGGTTTACTTGATCCAGAATTTGAAGAACAAGTTATCGGACAAGCTGAAGTTCGTCAAACATTCAAAGTTTCTAAAGTTgg                                                       <  1:38810/149‑1 (MQ=255)                                                                                         aaaTGTTGATATGCGTTTACACAGAGTTATTTATAATGTTATCGAAGAAATTGAATCAGCGATGAAAGGTTTACTTGATCCAGAATTTGAAGAACAAGTTATCGGACAAGCTGAAGTTCGTCAAACATTCAAAGTTTCTAAAGTTGGTa                                                     >  1:91946/1‑149 (MQ=255)                                                                                           aTGTTGATATGCGTTTACACAGAGTTATTTATAATGTTATCGAAGAAATTGAATCAGCGATGAAAGGTTTACTTGATCCAGAATTTGAAGAACAAGTTATCGGACAAGCTGAAGTTCGTCAAACATTCAAAGTTTCTAAAGTTGGTACt                                                   >  1:131038/1‑149 (MQ=255)                                                                                                      cGTTTACACAGAGTTATTTATAATGTTATCGAAGAAATTGAATCAGCGATGAAAGGTTTACTTGATCCAGAATTTGAAGAACAAGTTATCGGACAAGCTGAAGTTCGTCAAACATTCAAAGTTTCTAAAGTTGGTACTATTGCTGGatg                                        <  1:31739/149‑1 (MQ=255)                                                                                                         ttACACAGAGTTATTTATAATGTTATCGAAGAAATTGAATCAGCGATGAAAGGTTTACTTGATCCAGAATTTGAAGAACAAGTTATCGGACAAGCTGAAGTTCGTCAAACATTCAAAGTTTCTAAAGTTGGTACTATTGCTGGatgtta                                     >  2:185179/1‑149 (MQ=255)                                                                                                         ttACACAGAGTTATTTATAATGTTATCGAAGAAATTGAATCAGCGATGAAAGGTTTACTTGATCCAGAATTTGAAGAACAAGTTATCGGACAAGCTGAAGTTCGTCAAACATTCAAAGTTTCTAAAGTTGGTACTATTGCTGGatgtta                                     >  2:222076/1‑149 (MQ=255)                                                                                                          tACACAGAGTTATTTATAATGTTATCGAAGAAATTGAATCAGCGATGAAAGGTTTACTTGATCCAGAATTTGAAGAACAAGTTATCGGACAAGCTGAAGTTCGTCAAACATTCAAAGTTTCTAAAGTTGGTACTATTGCTGGatgttat                                    <  2:242314/149‑1 (MQ=255)                                                                                                          tACACAGAGTTATTTATAATGTTATCGAAGAAATTGAATCAGCGATGAAAGGTTTACTTGATCCAGAATTTGAAGAACAAGTTATCGGACAAGCTGAAGTTCGTCAAACATTCAAAGTTTCTAAAGTTGGTACTATTGCTGGatgttat                                    <  2:278851/149‑1 (MQ=255)                                                                                                                       ttatAATGTTATCGAAGAAATTGAATCAGCGATGAAAGGTTTACTTGATCCAGAATTTGAAGAACAAGTTATCGGACAAGCTGAAGTTCGTCAAACATTCAAAGTTTCTAAAGTTGGTACTATTGCTGGATGTTATGTTACTGAAGGTa                       >  2:302438/1‑149 (MQ=255)                                                                                                                                    gaagaaATTGAATCAGCGATGAAAGGTTTACTTGATCCAGAATTTGAAGAACAAGTTATCGGACAAGCTGAAGTTCGTCAAACATTCAAAGTTTCTAAAGTTGGTACTATTGCTGGATGTTATGTTACTGAAGGTAAAATTACGCGaaa          >  2:41979/1‑149 (MQ=255)                                                                                                                                       gaaATTGAATCAGCGATGAAAGGTTTACTTGATCCAGAATTTGAAGAACAAGTTATCGGACAAGCTGAAGTTCGTCAAACATTCAAAGTTTCTAAAGTTGGTACTATTGCTGGATGTTATGTTACTGAAGGTAAAATTACGCGAAATGc       >  1:36361/1‑149 (MQ=255)                                                                                                                                       gaaATTGAATCAGCGATGAAAGGTTTACTTGATCCAGAATTTGAAGAACAAGTTATCGGACAAGCTGAAGTTCGTCAAACATTCAAAGTTTCTAAAGTTGGTACTATTGCTGGATGTTATGTTACTGAAGGTAAAATTACGCGAAATGc       >  1:8007/1‑149 (MQ=255)                                                                                                                                         aaTTGAATCAGCGATGAAAGGTTTACTTGATCCAGAATTTGAAGAACAAGTTATCGGACAAGCTGAAGTTCGTCAAACATTCAAAGTTTCTAAAGTTGGTACTATTGCTGGATGTTATGTTACTGAAGGTAAAATTACGCGAAATGCTg     <  2:255793/149‑1 (MQ=255)                                                                                                                                            tGAATCAGCGATGAAAGGTTTACTTGATCCAGAATTTGAAGAACAAGTTATCGGACAAGCTGAAGTTCGTCAAACATTCAAAGTTTCTAAAGTTGGTACTATTGCTGGATGTTATGTTACTGAAGGTAAAATTACGCGAAATGCTGgtg  <  1:119241/149‑1 (MQ=255)                                                                                                                                             |                                                                                                                                                    ACGTGACACTTGCTAATGCCTCAAATGGTATTATCATTGGTTTCAATGTTCGTCCAGACAGTGGTGCAAAACGTGCTGCAGAAGCTGAAAATGTTGATATGCGTTTACACAGAGTTATTTATAATGTTATCGAAGAAATTGAATCAGCGATGAAAGGTTTACTTGATCCAGAATTTGAAGAACAAGTTATCGGACAAGCTGAAGTTCGTCAAACATTCAAAGTTTCTAAAGTTGGTACTATTGCTGGATGTTATGTTACTGAAGGTAAAATTACGCGAAATGCTGGTG  >  CP000730/1291977‑1292264

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑