Predicted mutation
evidence seq id position mutation annotation gene description
RA CP000730 36,378 C→T L58L (TTG→TTA)  tpn ← IS431mec transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073036,3780CT89.0% 1049.9 / 122.9 566L58L (TTG→TTA) tpnIS431mec transposase
Reads supporting (aligned to +/- strand):  ref base C (25/36);  new base T (255/241);  total (288/278)
Fisher's exact test for biased strand distribution p-value = 1.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.92e-01

Only 100 of 566 total aligned reads displayed.

TGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTA‑CGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAA‑TTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGT  >  CP000730/36232‑36523
                                                                                                                                                   |                                                                                                                                                  
    tACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATA‑CGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTTTTCCAAATTTGATATAAAA‑t                                                                                                                                              >  1:595745/1‑148 (MQ=255)
        taaCCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATA‑CGTCTCATCTATATGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAA‑TTggg                                                                                                                                          <  1:148856/147‑1 (MQ=11)
         aaCCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTA‑CGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAA‑TTgggg                                                                                                                                         <  1:338852/148‑1 (MQ=255)
          actaaaTATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATA‑CGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAA‑TTGGGGc                                                                                                                                        <  2:70973/145‑1 (MQ=255)
          aCCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATA‑CGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAA‑TTGGGGc                                                                                                                                        <  2:477327/148‑1 (MQ=255)
           ccAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATA‑CGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAA‑TTGGGGCa                                                                                                                                       <  1:107875/148‑1 (MQ=255)
            cAAATATCTAATGTATGTCCCTCTGCAAACATGTGACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTA‑CGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTCCCAACTTTGATCTAAAA‑TTGGGGCag                                                                                                                                      >  1:463882/1‑147 (MQ=11)
                 atCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATA‑CGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAA‑TTGGGGCATATTCt                                                                                                                                 <  1:32444/148‑1 (MQ=255)
                  tCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTA‑CGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAA‑TTGGGGCATATTCtt                                                                                                                                <  1:80951/148‑1 (MQ=37)
                   cTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATA‑CGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTTTTCCTAATTTTATATAAAC‑TGAGGGCATTATCTTg                                                                                                                               >  2:316131/1‑148 (MQ=11)
                     aaTGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATA‑CGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAA‑TTGGGGCATATTCTTGaa                                                                                                                             >  2:596018/1‑148 (MQ=255)
                      atgtatgtCCCTCTGCATCAATGGGACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTA‑CGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAA‑TTGGGGCATATTCTTGAAc                                                                                                                            >  1:312849/1‑148 (MQ=255)
                      atgtatgtCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTA‑CGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTTTTCCAAATTTGATACAAAA‑TTGGGGCATATTGTTGaag                                                                                                                            >  1:279880/1‑147 (MQ=255)
                         tatgtCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATA‑CGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAA‑TTGGGGCATATTCTTGAACCCa                                                                                                                         <  1:358511/148‑1 (MQ=255)
                           tgtCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATA‑CGTCTCATCAATACGCCATTTGTAATAAGCGTTTTTATGCTTTTTCTTCCAAATTTGATATAAAA‑TTGGGGCATATTCTTGAACCCAAc                                                                                                                       <  1:417364/148‑1 (MQ=255)
                           tgtCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTA‑CGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAA‑TTGGGGCATATTCTTGAACCCAAc                                                                                                                       >  2:231173/1‑148 (MQ=37)
                           tgtCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCATTTTATTTTGATGTA‑CGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTTATATAAAA‑TTGGGGCATATTTTTGAActataa                                                                                                                       >  2:36346/1‑143 (MQ=11)
                            ttCCCTCTGCATCAATGGCACGATATCAATAGCTCCATTTTTCTTTTATTTTGATGTA‑CGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAA‑TTGGGGCATATTCTTGAACCCAACg                                                                                                                      <  1:245362/147‑1 (MQ=255)
                                    gtaTCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATA‑CGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAA‑TTGGGGCATATTCTTGAACCCAACGGTAGATCg                                                                                                              <  2:147448/146‑1 (MQ=255)
                                     taTCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATA‑CGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAA‑TTGGGGCATATTCTTGAACCCAACGGTAGATCGt                                                                                                             >  1:412165/2‑148 (MQ=255)
                                       tCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTA‑CGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAA‑TTGGGGCATATTCTTGAACCCAACGGTAGACCGTTg                                                                                                           <  2:265866/148‑1 (MQ=40)
                                       tCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATA‑CGTTTCGTCACTACGCCATTTGTAATGAGCTTTTTTATGGTTTTTCTTGCAAATTTGTTGTAAAA‑TTTGGGCTTATTCTTGAACGCATCgtttgatggttg                                                                                                           >  2:302375/1‑137 (MQ=11)
                                         aaTGGCCCCATATAAATAGCTCCTTTTTCCTTTCATTTATATGTATCGCCGC‑CCAATACGCCAATTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAA‑TTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGaa                                                                                                         <  1:394601/148‑1 (MQ=14)
                                            ggCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTA‑CGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAA‑TTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAatga                                                                                                      <  2:293685/148‑1 (MQ=18)
                                              cACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTA‑CGTCCCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAA‑TTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAatgatg                                                                                                    <  2:603732/148‑1 (MQ=21)
                                                cgtaataAATACCTCCATTTTCCTTTTCTCTAGAGATA‑CATCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAA‑TTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGaa                                                                                                  <  1:485228/144‑1 (MQ=11)
                                                cGATATAAATAGCTCCATTTTCCTTTTATTTTGATATA‑CGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAA‑TTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGaa                                                                                                  <  1:208780/148‑1 (MQ=255)
                                                 gATATAAATAGCTCCATTTTCCTTTTATTTTGATATA‑CGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAA‑TTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGCAc                                                                                                 >  2:423014/1‑148 (MQ=255)
                                                  atatAAATAGCTCCATTTTCCTTTTATTTTGATGTA‑CGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAA‑TTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACg                                                                                                <  1:42583/148‑1 (MQ=39)
                                                    ataAATAGCTCCATTTTCCTTTTATTTTGATGTA‑CGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAA‑TTGGGGCATATTCTTGAACCCAACGGTAGACAGTTGAATGATGACCGtt                                                                                              >  2:277540/1‑148 (MQ=255)
                                                    ataAATAGCTCCATTTTCCTTTTATTTTGATATA‑CGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAA‑TTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGtt                                                                                              >  1:30698/1‑148 (MQ=255)
                                                    ataAATAGCTCCATTTTCCTTTTATTTTGATATA‑CGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAA‑TTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGtt                                                                                              >  1:254378/1‑148 (MQ=255)
                                                    ataAATAGCTCCATTTTCCTTTTATTTTGATATA‑CGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAA‑TTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGtt                                                                                              <  1:443221/148‑1 (MQ=255)
                                                     taAATAGCTCCATTTTCCTTTTATTTTGATATA‑CGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAA‑TTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGgtt                                                                                             >  2:348009/1‑148 (MQ=255)
                                                     taAATAGCTCCATGTTCCTTTCATTTTTGTATA‑CGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAA‑TTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGttt                                                                                             <  2:518206/148‑1 (MQ=11)
                                                      aaaTAGCTCCATTTTCCTTTTATTATGATATA‑CGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCACATTTGCTATAAAA‑TTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTa                                                                                            <  1:560553/148‑1 (MQ=11)
                                                         tAGCTCCATTTTCCTTTTATTTTGATGTA‑CGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAA‑TTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTacac                                                                                         >  2:568857/1‑148 (MQ=21)
                                                         tAGCTCCATTTTCCTTTTATTTTGATATA‑CGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAA‑TTGGGGCATATTCTTGAACCCAACGGTCGATCGTTGAATTATGAACGTTTacca                                                                                         >  1:222258/1‑146 (MQ=11)
                                                         tAGCTCCATTTTCCTTTTATTTTGATATA‑CGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAA‑TTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTacac                                                                                         >  1:525795/1‑148 (MQ=255)
                                                          aGCTCCATTTTCCTTTTATTTTTATATA‑CGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAA‑TTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcacc                                                                                        <  1:100207/148‑1 (MQ=255)
                                                           gCTCCATTTTCCTTTTATTTTGATGTA‑CGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATCTCAAT‑TTGGGGAATATTCTTGAACCCAACGGTGGACCGTTGAATGATGAACGTATAcacca                                                                                       >  2:432671/1‑148 (MQ=11)
                                                           gCTCCATTTTCCTTTTATTTTGATGTA‑CGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAA‑TTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTacacga                                                                                       >  1:535293/1‑146 (MQ=25)
                                                           gCTCCATTTTCCTTTTATTTTGATGTA‑CGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAA‑TTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTAcacca                                                                                       <  2:552404/148‑1 (MQ=18)
                                                            cTCCATTTTCCTTTTATTTTGATGTA‑CGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAA‑TTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTAcaccac                                                                                      >  1:511371/1‑148 (MQ=21)
                                                             tCCATTTTCCTTTTATTTTGATGTA‑CGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAA‑TTGGGGCATATTCTTGAACCCAAAGGTGGACCGTTGAATGATGAACGTTTacacacag                                                                                     >  1:601113/1‑144 (MQ=255)
                                                             tCCATTTTCCTTTTATTTTGATGTA‑CGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAA‑TTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACacgacg                                                                                     >  1:548771/1‑148 (MQ=255)
                                                             tCCATTTTCCTTTTATTTTGATATA‑CGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAA‑TTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACg                                                                                     >  1:17033/1‑148 (MQ=255)
                                                              ccATTTTCCTTTTATTTTGATGTA‑CGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAA‑TTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGt                                                                                    >  2:44577/1‑148 (MQ=25)
                                                              ccATTTTCCTTTTATTTTGATATA‑CGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAA‑TTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGt                                                                                    >  2:618535/1‑148 (MQ=255)
                                                              ccATTTTCCTTTTATTTTGATATA‑CGTCTCATCAATACGCCATTTGTAATAACCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAA‑TTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACgc                                                                                    >  2:334930/1‑147 (MQ=11)
                                                               cATTTTCCTTTTATTTTGATGTA‑CGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTAGTCTTTTTCTTCATATTTTGTTTTAAAA‑TTGGGACATATTATTGATCCCACCTGTAGACCGTTGAAGCTTGCACGTTTACTCCACGtt                                                                                   >  2:39807/1‑148 (MQ=11)
                                                                 ttttCCTTTTATTTTGATGTA‑CGTCTCATCAATACGCCATTTGTAATCAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAA‑TTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGtggtgtaaacgttca                                                                                 <  1:38365/148‑15 (MQ=11)
                                                                 ttttCCTTTTATTTTGATGTA‑CGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAA‑TTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTcc                                                                                 <  2:378450/148‑1 (MQ=18)
                                                                   ttCCTTTTATTTTTGTGTA‑CGTCTCATTAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAA‑TTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCt                                                                               <  2:4917/148‑1 (MQ=255)
                                                                     ccTTTTATTTTGATATA‑CGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAA‑TTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTa                                                                             <  1:300012/148‑1 (MQ=255)
                                                                     ccTTTTATTTTGATATA‑CGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAA‑TTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTa                                                                             <  2:188179/148‑1 (MQ=11)
                                                                      cTTTTATTTTGATGTA‑CGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAA‑TTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTaa                                                                            <  2:53870/148‑1 (MQ=21)
                                                                        tttATTTTGATGTA‑CGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAA‑TTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAata                                                                          >  1:372442/1‑148 (MQ=25)
                                                                          tATTTTGACATA‑CGTTTCATCAATACGGCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAA‑TTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATAtt                                                                        <  2:220590/148‑1 (MQ=11)
                                                                           aTTTTGATGTA‑CGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAA‑TTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATAttt                                                                       >  1:541868/1‑148 (MQ=25)
                                                                            ttttGATGTA‑CGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAA‑TTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTc                                                                      <  1:434118/148‑1 (MQ=40)
                                                                            ttttGATATA‑CGTCTCATCATTACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAA‑TTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTc                                                                      <  1:26158/148‑1 (MQ=11)
                                                                            ttttGATATA‑CGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAA‑TTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCGCGTTCTCTTAATATTTc                                                                      <  2:405054/148‑1 (MQ=11)
                                                                             tttGATATA‑CGTTTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAA‑TTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCa                                                                     <  2:389842/148‑1 (MQ=11)
                                                                              ttGATGTA‑CGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAA‑TTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAg                                                                    >  1:182144/1‑148 (MQ=40)
                                                                              ttGATATA‑CGTCTCATCAATACGCCATTTGTAATAAGAAATTTTATGCTTTTTCTTCCAAATTTGATATAAAA‑TTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAg                                                                    >  1:624526/1‑148 (MQ=11)
                                                                               tGATGTA‑CGTCTCATCAATACGCCAATGGTAATAAGCTTTTTTATGCTTTTTCTTCCGAATTTGATATAAAA‑ATAGGGCATATGCTTGAACCCAACGGTAGACAGCTTACGGATGAACGTTTACACCACGTTCCCTTAATATTTCAGa                                                                   >  2:168178/1‑148 (MQ=11)
                                                                               gggtGTA‑CGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAA‑TTGGGGCATATTCTTTAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGa                                                                   <  2:81224/145‑1 (MQ=255)
                                                                                gATGTA‑CGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAA‑TTGGGGCATATACTTGAACCAAACGGTAGACCGTTGAATAATGAACGTTTACACCACGTTCCCTTAATATTTCAgct                                                                  >  2:327305/1‑146 (MQ=255)
                                                                                 atatA‑CGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTTTTTTTTTTTTTCCAAATTTGATATAAAA‑TTGGGTCATATTCTTGAACCCAACGGTAGATCGTTGCATTCTGACCGTTTTCACCACGTTCTCTTAATATTTctaata                                                                 >  2:509851/4‑143 (MQ=11)
                                                                                 atatA‑CGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAA‑TTGGGGTATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGata                                                                 <  1:577882/145‑1 (MQ=11)
                                                                                    tA‑CGTCTCATCAATACGCCATTTGTAATAATCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAA‑TTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCGCGTTCTCTTAATATTTCAGatatat                                                              <  1:97506/148‑1 (MQ=11)
                                                                                    tA‑CGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAA‑TTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGatatat                                                              >  1:289278/1‑148 (MQ=255)
                                                                                    tA‑CGTCTCATCAATACGACCTTTGTAATAAGCTTTTTTCTGCTTTTTCTTCCAAATTTCATATAAAA‑TTGGGGCATATTCTTTAACCCAACGGTCGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGacatat                                                              >  2:250229/1‑148 (MQ=11)
                                                                                     a‑CGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAA‑TTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATc                                                             >  2:239337/1‑148 (MQ=255)
                                                                                       cGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTGTGCTTTTTCTTCCAAATTTGATATAAAA‑TTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCCCGTTCCCTTAATATTTCAGATATATCa                                                            >  1:235916/1‑148 (MQ=255)
                                                                                        gTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAA‑TTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCAc                                                           <  1:119821/148‑1 (MQ=255)
                                                                                         tctcATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAA‑TTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACg                                                          >  2:341949/1‑148 (MQ=21)
                                                                                         tctcATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTAATATAAAA‑TTAGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAAACCACGTCATCTTACTATTTCAGCTATACCACg                                                          >  2:468173/1‑148 (MQ=11)
                                                                                         tctcATAAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAA‑TTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACg                                                          <  1:293293/148‑1 (MQ=255)
                                                                                          ctcATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAA‑TTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGa                                                         <  2:182047/148‑1 (MQ=255)
                                                                                             atcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAA‑TTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATaa                                                      <  2:587227/148‑1 (MQ=40)
                                                                                              tcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAACTCGGGACAT‑TTCTTGAACTCAACGGTAGACCGTTGAACGATGAACGTTTACACCACGTTCCCTTACTATTTCAGATATATCACGATAAc                                                     >  2:572598/1‑148 (MQ=11)
                                                                                              tcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAA‑TTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTCCCCCACGTTCCCTTAATATTTCAGATATATCACGATagc                                                     >  1:448190/1‑146 (MQ=255)
                                                                                              tcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAA‑TTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATagc                                                     >  1:132006/1‑146 (MQ=255)
                                                                                                 aTACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAA‑TTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCa                                                  <  2:457160/148‑1 (MQ=11)
                                                                                                   cccccATTTGTATTAAGCTTTTTTATGCTTTTTCTTCCAAATGTGATATAAAA‑TTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAAt                                                <  2:155414/145‑1 (MQ=255)
                                                                                                   aCGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAA‑TTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAAt                                                <  1:63739/148‑1 (MQ=11)
                                                                                                     gCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCACATTTGATATAAAA‑TTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATgc                                              <  2:14472/148‑2 (MQ=11)
                                                                                                     gCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAA‑TTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATgc                                              <  2:115939/148‑2 (MQ=11)
                                                                                                      ccATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAA‑TTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAAtcaa                                             >  1:472600/1‑145 (MQ=21)
                                                                                                         tttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAA‑TTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATGCatat                                          >  1:200605/1‑148 (MQ=11)
                                                                                                            gTAATAATCTCTTTTATGCTTCTTCTTCCAAATTTGATACAAAA‑TTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATTTCACGATAACTCAATGTATATCtt                                       <  1:494219/148‑1 (MQ=255)
                                                                                                            gTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAA‑TTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGCATATCtt                                       <  1:195189/148‑1 (MQ=2)
                                                                                                             taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAA‑TTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATGCATATCTTa                                      <  1:9311/148‑1 (MQ=11)
                                                                                                             taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAA‑TTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGCATATCTTa                                      <  2:450970/148‑1 (MQ=14)
                                                                                                                                     ttcCAAATTTGCTACAAAA‑TTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGa              <  2:2080/148‑1 (MQ=25)
                                                                                                                                          aaTTTGATACAAAA‑TTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACa         <  2:484975/148‑1 (MQ=35)
                                                                                                                                             ttGATACAAAA‑TGGGGGCATATTCTTGCACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGAAATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATcc      <  2:132970/148‑1 (MQ=255)
                                                                                                                                                 tACAAAA‑TTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGt  <  1:154403/148‑1 (MQ=35)
                                                                                                                                                   |                                                                                                                                                  
TGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTA‑CGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAA‑TTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGT  >  CP000730/36232‑36523

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑