Predicted mutation
evidence seq id position mutation annotation gene description
RA CP000730 1,744,362 G→A H104Y (CAC→TAC)  apt ← adenine phosphoribosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,744,3620GA97.9% 130.1 / ‑5.0 47H104Y (CAC→TAC) aptadenine phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (29/17);  minor base C (0/1);  total (29/18)
Fisher's exact test for biased strand distribution p-value = 3.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.43e-01

TTCAATTCAATTATAAATGCAATACCTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACG  >  CP000730/1744220‑1744500
                                                                                                                                              |                                                                                                                                          
ttcaattcaaTTATAAATGCAATACCTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTg                                                                                                                                       >  2:613464/1‑148 (MQ=255)
 tcaattcaatTATAAATGCAATACCTACTACGATACCGCCTAATTTTTAAACTAATTTTATTGCTGCTTCAATCGTACAACCAGTAGCTAATAAATCATCTGTAATTAACCAACGTTGACCAGGTTTAATTGAATCTTTGTAGATTGt                                                                                                                                      >  2:173762/1‑148 (MQ=255)
  caattccattATATATGCAATTCCCACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCATTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGtt                                                                                                                                     <  1:274794/148‑1 (MQ=255)
          ttATAATTGCAATACCTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACAtt                                                                                                                             <  1:476985/148‑1 (MQ=255)
           tattAATGCAATACCTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACAttt                                                                                                                            <  2:151036/148‑1 (MQ=255)
             taAATGCAATACCTACTACGATACCGCCTAATTTTTTTTTTTTTTTTTTTACTGCTACAATCGTACCACCATTAGCTAATAAATCATCTGTAATTAACACACGTTCCCCTGGTTTAATTGCATCTTTCTACATTCTTCAAACAttttt                                                                                                                          >  2:60317/1‑146 (MQ=255)
             taAATGCAATACCTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGt                                                                                                                          >  2:627084/1‑148 (MQ=255)
              aaaTGCAATACCTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTa                                                                                                                         <  2:283451/148‑1 (MQ=255)
              aaaTGCAATACCTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGGTAAAACATTTGTa                                                                                                                         >  2:337126/1‑148 (MQ=255)
                aTGCAATACCTACTACGATGATGGCTAATAATACAACTTCTTTTTTAAGAGCAACAATCGTACCACCCGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTAcc                                                                                                                       <  1:454854/148‑1 (MQ=255)
                aTGCAATACCTACTACGATACCGCTTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTAcc                                                                                                                       >  1:350356/1‑148 (MQ=255)
                   cAATACCTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCata                                                                                                                    >  1:362948/1‑148 (MQ=255)
                    aaTACCTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTGGACCTGATTTAATTGCATATTTGTACATTGTTAAAACATTTGTACCattt                                                                                                                   >  2:614691/1‑146 (MQ=255)
                        ccTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCAGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACGTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTa                                                                                                               >  2:416308/1‑148 (MQ=255)
                         ctactaCGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAg                                                                                                              >  1:540573/1‑148 (MQ=255)
                                    ccGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCAt                                                                                                   >  1:387763/1‑148 (MQ=255)
                                      gCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTCCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATaa                                                                                                 <  2:463158/148‑1 (MQ=255)
                                      gCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATaa                                                                                                 <  1:308577/148‑1 (MQ=255)
                                        cTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTTCCATATTCTAGGTCATACTCATAACg                                                                                               >  2:503756/1‑148 (MQ=255)
                                               ttCAACTAATTTTATTGCTGCTTCAATCGTCATGACATGGGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACt                                                                                        >  1:150542/1‑148 (MQ=255)
                                                 cAACTAATTTTATTGCTGCTTCAATCGTACCTCCTTAAGCTAATAATTCATCTGTAATTAACACACGTTTACCTTGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGTTCATACTCATATCGAATGACTTc                                                                                      <  2:379565/148‑1 (MQ=255)
                                                   aCTAATTTTATCGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACCCACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCAc                                                                                    <  1:597040/148‑1 (MQ=255)
                                                    cTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACg                                                                                   >  1:72477/1‑148 (MQ=255)
                                                            aTTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAAtt                                                                           >  1:288443/1‑148 (MQ=255)
                                                             ttGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGACATCCTCATAACGAATGACTTCACGAGGTAAttt                                                                          <  1:151215/148‑1 (MQ=255)
                                                                 tgctTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCct                                                                      >  2:248602/1‑148 (MQ=255)
                                                                 tgctTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCct                                                                      >  2:429842/1‑148 (MQ=255)
                                                                       aaTCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTTACGAGGTAATTTCCCTTCtttt                                                                >  1:490355/1‑148 (MQ=255)
                                                                          cGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTa                                                             <  2:225443/148‑1 (MQ=255)
                                                                               caccaGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAgg                                                        >  1:114109/1‑148 (MQ=255)
                                                                                 ccaGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTg                                                      <  1:412891/148‑1 (MQ=255)
                                                                                          aataaATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCaa                                             >  1:589121/1‑148 (MQ=255)
                                                                                              aaTCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATccc                                         <  2:72477/148‑1 (MQ=255)
                                                                                                tcatcTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTCGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCa                                       >  2:248616/1‑148 (MQ=255)
                                                                                                tcatcTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTCGTACATTCATAAAACATTTGTACCATATTATAGGTCATACTCCTAACGAATGACTTAACGAGGTAATTTCCATTCTTTTCTAACAGGTGCaaaacaaaagcaca                                       >  2:616699/1‑137 (MQ=255)
                                                                                                  atcTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCAtt                                     <  2:287430/148‑1 (MQ=255)
                                                                                                     tGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATACTAGGAAATAAACATAATTAAAGTATTGTGGTGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGaa                                  >  2:68087/1‑148 (MQ=255)
                                                                                                        aaTTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATaa                               >  2:621073/1‑148 (MQ=255)
                                                                                                           tAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCt                            <  1:97939/148‑1 (MQ=255)
                                                                                                             acacacGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTAc                          <  2:567612/148‑1 (MQ=255)
                                                                                                               acacGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACGATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAg                        >  2:336531/1‑148 (MQ=255)
                                                                                                                       aCCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCa                <  1:233560/148‑1 (MQ=255)
                                                                                                                           ggTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGa            <  1:597426/148‑1 (MQ=255)
                                                                                                                                 aTTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTACCAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCCATCATAAAGc      >  2:498408/1‑148 (MQ=255)
                                                                                                                                 aTTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGc      >  2:189342/1‑148 (MQ=255)
                                                                                                                                 aTTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTAACGAGGTAATTTCCATTCTTTTCCAACAGGTGCAAAGCCAATGCCCATTGAATAAGCTAAAGGTAAGCGAATGCTAAAGc      >  2:498528/1‑148 (MQ=255)
                                                                                                                                     cATCTTTGTACATTGTTAAAACAATTGTATCTCGTTCTAGGTCATACTCATACCGAATGCCTTCACGAGGTAATTTCCCTTCTTTTCTAACAGCTGCAAAGCCAATCCCCATTGAATAAGCTACAGGTCAGCCAATGATaaatctaca  >  2:135570/1‑142 (MQ=255)
                                                                                                                                              |                                                                                                                                          
TTCAATTCAATTATAAATGCAATACCTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACG  >  CP000730/1744220‑1744500

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑