Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	2,010,363	C→T	C146Y (TGT→TAT)	ygaD ←	ABC superfamily ATP binding cassette transporter, membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,010,363	0	C	T	100.0%	171.3 / NA	54	C146Y (TGT→TAT)	ygaD	ABC superfamily ATP binding cassette transporter, membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/31);  total (23/31)

GATCTTTCACGTGTCAATTTTCTTAATCTTCCAAAGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATACAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACTTAAAGCTTGTAAATGGTTGTATA  >  CP000730/2010222‑2010501                                                                                                                                              |                                                                                                                                           gagcgttCACGTGTAAATTTTCTTAACCTGCCAAAGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTAAATTTCAAATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTa                                                                                                                                      <  2:365294/143‑1 (MQ=255)   cttgtCATGTGCCAAGTTTCTTAATCTTCCAACGAAAACGTCCACCGTTAAAAGGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTAAATTTCACATCTAAAAAGAACATTATGGAGAGTGCAATAATAATTGTTATATAATCTAAc                                                                                                                                    <  2:484715/144‑1 (MQ=255)           gtttctaTTTTCTTTATCTTTCATAGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATAtt                                                                                                                            <  1:45254/142‑1 (MQ=255)           gtgtCAATTTTCTTAATCTTCCAAAGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATAtt                                                                                                                            <  2:341980/148‑1 (MQ=255)            tgtCAATTTTCTTAATCTTCCAAAGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTc                                                                                                                           <  1:50084/148‑1 (MQ=255)             gttaaTTGTCTTAATCTGCCAAAGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCa                                                                                                                          <  1:611782/145‑1 (MQ=255)             gtCAATTTTCTTAATCTTCCAAAGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCa                                                                                                                          >  1:367025/1‑148 (MQ=255)                    ttCTTAATCTTCCAAAGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAAccc                                                                                                                   >  2:131582/1‑148 (MQ=255)                    tcattcatagtacAAAAAAAAAGTTGACGGTTAAGAGGTTAGATTGGATGATAAAGAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAAAAATTGTTATATAATCTAACCAAATATTCATTCAcca                                                                                                                   >  2:527402/13‑147 (MQ=255)                      cTTAATCTTCCAAAGAAAACGTACAACGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGCTAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCgg                                                                                                                 <  1:620705/148‑1 (MQ=255)                         aaTCTTCCAAAGAAAACGTAAACAGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTGAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTa                                                                                                              >  2:124444/1‑148 (MQ=255)                                 aaaGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATAGTTATATAATCTAACCAAATATTCATTAACCCTGTTAAAATTaaa                                                                                                      >  2:433365/1‑148 (MQ=255)                                      aaaCGTACACCGTAAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGCTAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCttt                                                                                                 <  2:396609/148‑1 (MQ=255)                                                     aaatgtaaaatgGGAAGATAAACAGTGCTGCTAAAGTCACTTTCACAATTACATATGTGATTATGGATATTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGCTTGTTCAAcatc                                                                                  <  1:429200/148‑1 (MQ=255)                                                      aatgtaaaatgGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAAcatca                                                                                 >  2:337912/1‑148 (MQ=255)                                                            aaatgGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAAta                                                                           >  2:404867/1‑147 (MQ=255)                                                             aatgGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCa                                                                          >  2:597095/1‑148 (MQ=255)                                                             aatgGGAAGAAAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAATATTATGGTTAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCa                                                                          <  2:243045/148‑1 (MQ=255)                                                               tgGGAAGATAACCCGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCAct                                                                        <  1:210631/148‑1 (MQ=255)                                                                   aaGATAAACAGTGCTGCAGAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTATGTTCAACATCATTAATCACTCTat                                                                    >  1:461587/1‑147 (MQ=255)                                                                       tAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGatat                                                                >  1:508839/1‑148 (MQ=255)                                                                         aaCAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATACCTCTAGATATTa                                                              >  2:464468/1‑148 (MQ=255)                                                                         aaCAGTGCTGCTAAAGTCAAGTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTa                                                              >  1:401376/1‑148 (MQ=255)                                                                              ttctgctAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGa                                                         <  2:607009/146‑1 (MQ=255)                                                                              tgctgcTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGa                                                         <  1:576027/148‑1 (MQ=255)                                                                              tgctgcTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGa                                                         <  1:611162/148‑1 (MQ=255)                                                                                ctgctAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGGcc                                                       >  1:187447/1‑148 (MQ=255)                                                                                ctgctAAAGTCAATGTCACATCTAAAAAGAACATTATGGATAGGGCAATAATAATTGTTATATAATCTGACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACGTCATTAATCACTCTAGATATTACTTGAcc                                                       <  1:439516/148‑1 (MQ=255)                                                                                  gctAAAGTAAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTa                                                     <  1:44016/148‑1 (MQ=255)                                                                                      aaGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTg                                                 <  1:540402/148‑1 (MQ=255)                                                                                       aGTCAATTCCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGa                                                <  1:331190/148‑1 (MQ=255)                                                                                        gtaaaTTTCAAATCTAAAAAGAACATTATTGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGat                                               <  2:600319/145‑1 (MQ=255)                                                                                           aaTTTCACATCTCAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGattat                                            <  1:551717/148‑1 (MQ=255)                                                                                           aaTTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGattat                                            <  1:565691/148‑1 (MQ=255)                                                                                            aTTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATTATTAATCACACTAGATATtatttgatttatttgattcta                                           >  2:357944/1‑145 (MQ=255)                                                                                            aTTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGattatt                                           <  2:274721/148‑1 (MQ=255)                                                                                            aTTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGattatt                                           <  2:274668/148‑1 (MQ=255)                                                                                              ttCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAg                                         <  1:608937/148‑1 (MQ=255)                                                                                              ttCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAg                                         >  2:523083/1‑148 (MQ=255)                                                                                              ttCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTAGATAAGCTAACCAAATATTCATTGACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCCAGATATTACTTGACCTACTTGATTATTAg                                         >  2:526846/1‑148 (MQ=255)                                                                                                             aaCATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCAc                          <  1:337912/148‑1 (MQ=255)                                                                                                                  tATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACTTaaa                     <  2:367025/148‑1 (MQ=255)                                                                                                                     gggtAGTGCAATAATAATTGTTATATAATCTAACCAAATATTAATTAACCCAGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACTTAAAGCt                  >  2:624235/4‑148 (MQ=255)                                                                                                                      gATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACTTAAAGCtt                 >  1:492970/1‑148 (MQ=255)                                                                                                                      gATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACTTAAAGCtt                 >  2:405595/1‑148 (MQ=255)                                                                                                                      gATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACTTAAAGCtt                 >  2:405559/1‑148 (MQ=255)                                                                                                                       aTAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACTTAAAGCTTg                >  2:505236/1‑148 (MQ=255)                                                                                                                            gCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGGTAAAAGGAAATCTTTTGTTTGTTCAACATCATTAATCACACTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACTTAAATATTGTAAAt           >  1:475012/1‑148 (MQ=255)                                                                                                                               ataaatatTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCAATAATCACTCTAGCTATTACTTGACCTACTTTATTATTAGCATAAAATCTCGCACTTAAAGCTTGTAAATGGt        <  2:64669/142‑1 (MQ=255)                                                                                                                                 aataatTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTCCTTGACCTACTTGATTATTAGCATAAAATCTCGCACTTAAAGCTTGTAAATGGTTg      <  2:295863/148‑1 (MQ=255)                                                                                                                                   tagttGTTATATAATCTAATCAAATATTCATTAACCCGGTTAATATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACTTAAAGCTTGTAAATGGTTGta    <  1:182548/145‑1 (MQ=255)                                                                                                                                   taatTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACTTAAAGCTTGTAAATGGTTGta    <  2:520667/148‑1 (MQ=255)                                                                                                                                   taatTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACTTAAAGCTTGTAAATGGTTGta    <  2:523491/148‑1 (MQ=255)                                                                                                                                     atTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACTTAAAGCTTGTAAATGGTTGtata  >  1:29361/1‑148 (MQ=255)                                                                                                                                              |                                                                                                                                           GATCTTTCACGTGTCAATTTTCTTAATCTTCCAAAGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATACAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACTTAAAGCTTGTAAATGGTTGTATA  >  CP000730/2010222‑2010501

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑