Predicted mutation
evidence seq id position mutation annotation gene description
RA CP000730 195,286 T→A intergenic (‑97/‑82) USA300HOU_0183 ← / → USA300HOU_0184 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730195,2860TA98.0% 157.3 / ‑5.2 52intergenic (‑97/‑82)USA300HOU_0183/USA300HOU_0184hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (1/0);  major base A (27/23);  minor base C (0/1);  total (28/24)
Fisher's exact test for biased strand distribution p-value = 4.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01

TCTTTTTGTTTCTAGCTAAACTTGCTACGACAGATTCTTTATCCATAATGATAGCCCCCTATATATATGTTTATTTACTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTTAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCTTATTAATGACATTATTTTTATTAGAAGCTAACAATCTTGATTTTGCATCAACGAAAGAAGAACTAGAA  >  CP000730/195144‑195430
                                                                                                                                               |                                                                                                                                                
tCTTTTTGTTTCTAGCTAAACTTGCTACGACAGATTCTTTATCCATAATGATAGCCCCCTATATATATGTTTATTTACTTATACCCTAACATGATTTTTATATTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTaaaaaa                                                                                                                                             >  1:314632/1‑148 (MQ=255)
tCTTTTTGTTTCTAGCTAAACTTGCTACGACAGATTCTTTATCCATAATGATAGCCCCCTATATATATGTTTATTTACTTATACCCTAACATGATTTTTATACTCTTTGTAAATATTTTTTACAGAATTTTATCTAAATTTTaataaa                                                                                                                                            >  1:171264/1‑148 (MQ=255)
 cTTTTTGTTTCTAGCTAAACTTGCTACGACAGATTCTTTATCCATAATGATAGCCCCCTATATATATGTTTATTTACTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAaaaaaa                                                                                                                                            <  1:189180/148‑1 (MQ=255)
          tCTAGCTAAACTTGCTACGACAGAGTCTTTATCCATAATGATAGCCCCCTATATATATGTTTATTTACTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTaa                                                                                                                                   >  1:314462/1‑148 (MQ=255)
              gCTAAACTTGCTACGACAGATTCTTTATCCATAATGATAGCCCCCTATATATATGTTTATTTACTTATACCTTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATc                                                                                                                               <  2:539247/148‑1 (MQ=255)
              gCTAAACTTGCTACGACAGATTCTTTATCCATAATGATAGCCCCCTATATATATGTTTATTTACTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATc                                                                                                                               <  1:295440/148‑1 (MQ=255)
               cTAAACTTGCTACGACAGATTCTTTATCCATAATGATAGCCCCCTATATATATGTTTATTTACTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAAATATCTTAATATc                                                                                                                               <  1:123158/148‑1 (MQ=255)
                tAAACTTGCTACGACAGATTCTTTATCCATAATGATAGCCCCCTATATATATGTTTATTTACTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCt                                                                                                                             >  1:369948/1‑148 (MQ=255)
                  aaCTTGCTACGACAGATTCTTTATCCATAATGATAGCCCCCTATATATATGTTTATTTACTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGCATTTTTTCTAACAATCAAAAAAAATATAtttggaaccacg                                                                                                                           >  1:296259/1‑138 (MQ=255)
                  aaCTTGCTACGACAGATTCTTTATCCATAATGATAGCCCCCTATATATATGTTTATTTACTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTg                                                                                                                           >  2:391323/1‑148 (MQ=255)
                            gACAGATTCTTTATCCATAATGATAGCCCCCTATATATATGTTTATTTACTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATa                                                                                                                 >  1:627924/1‑148 (MQ=255)
                               aGATTCTTTATCCATAATGATAGCCCCCTATATATATGTTTATTTACTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTTATCCGATAAGa                                                                                                              >  1:310314/1‑148 (MQ=255)
                                  ttCTTTATCCATAATGATAGCCCCCTATATATATGTTTATTTACTTATACCCTAACATGCTTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTATAAtacaaatagtatt                                                                                                           >  2:323456/1‑136 (MQ=255)
                                    cTTTATCCATAATGATAGCCCCCTATATATATGTTTATTTACTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATtat                                                                                                         <  1:100009/148‑1 (MQ=255)
                                          ccATAATGATACCCCCTTATATATATGTTTATTAACTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAat                                                                                                   <  2:32071/148‑1 (MQ=255)
                                             tAATGATAGCGCCCTATATATATGTTTATTTACTTATACCCTAACATGATTTTTTTTTTCTTTTCAATTATATTTTACAGAATTTTATCTAAATATTAGAATGTATATCTTAATATTCTTGTAATCCGCTAATAATTATAGTAACAtt                                                                                                >  2:278319/1‑148 (MQ=255)
                                               aTGATAGCCCCCTATATATATGTTTATTTACTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATAtttt                                                                                              >  1:152812/1‑148 (MQ=255)
                                                tGATAGCCCCCTATATATATGTTTATTTACTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATAttttt                                                                                             >  1:474555/1‑148 (MQ=255)
                                                  aTAGCCCCCTATATATATGTTTATTTACTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATATTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTc                                                                                           >  1:88833/1‑148 (MQ=255)
                                                      cccccTATATATATGTTTATTTACTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAccc                                                                                       >  2:32169/1‑148 (MQ=255)
                                                      cccccTATATATATGTTTATTTACTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAAcc                                                                                       >  2:392481/1‑148 (MQ=255)
                                                      cccccTATATATATGTTTATTTACTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAAAATTATAGTAATATTTTTTaaaac                                                                                       >  2:427659/1‑146 (MQ=255)
                                                           tatatataTGTTTATTTACTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGt                                                                                  <  1:521434/148‑1 (MQ=255)
                                                            atatatatGTTTATTTACTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGtt                                                                                 <  2:512436/148‑1 (MQ=255)
                                                                  atGTTTATTTACTTATACCCTAACATGATTTTTATAATCTTTGAAAATATATTTTACAGAATTTTATCTAACTATTAAAAAAAATATCTTAATATCCTTTTATTCCGATAAGAATTATAGTAGTTTTTTTTCAACCATTGTtatctga                                                                           >  1:162553/1‑144 (MQ=255)
                                                                    gTTTATTTACTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATaggagg                                                                         >  2:104724/1‑148 (MQ=255)
                                                                     tttatttaCTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGt                                                                        <  1:250825/148‑1 (MQ=255)
                                                                       tatttaCTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCt                                                                      >  2:356303/1‑148 (MQ=255)
                                                                        atttaCTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCtt                                                                     <  1:475676/148‑1 (MQ=255)
                                                                         tttaCTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCtta                                                                    >  2:135982/1‑148 (MQ=255)
                                                                         tttaCTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCtta                                                                    >  2:516862/1‑148 (MQ=255)
                                                                         gaaaagtacaCCCTAACATTAATTTTATACTTATAGAAAAAATATTCTACATAATTTTATAAAAAAAAAAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCtta                                                                    <  2:110802/139‑1 (MQ=255)
                                                                          ttaCTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAACTATTAAAAAACATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCCACCAATCTAATAGAGGGTCttat                                                                   >  2:219981/1‑148 (MQ=255)
                                                                              ttATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCTTATTAAt                                                               >  1:201524/1‑148 (MQ=255)
                                                                               tataCCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCTTATTAATg                                                              <  2:580617/148‑1 (MQ=255)
                                                                                  aCCCTAACCTGATTTTTATCCTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCTTATTAATGACa                                                           <  1:13084/148‑1 (MQ=255)
                                                                                  aCCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCTTATTAATGACa                                                           <  2:480320/148‑1 (MQ=255)
                                                                                  aCCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTAACTAAAAAATAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCTTATTAATGACa                                                           <  2:629721/148‑1 (MQ=255)
                                                                                    ccTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGATATAGGAGGTCTTATTAATTACttt                                                         >  2:194795/1‑148 (MQ=255)
                                                                                      tAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAACAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCTTATTAATTACattat                                                       >  2:193207/1‑148 (MQ=255)
                                                                                         ttccaTTTTTATACGCATCCAAATTAGAGTTGACTGAATTTTATCTAAATATTAAAAAAAATATCCTAATATCCTTGTATTCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCTTATTAATGACATTAtttt                                                    <  1:13016/144‑1 (MQ=255)
                                                                                           tGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCTTATTAATGACATTATTTtta                                                  <  2:629441/148‑1 (MQ=255)
                                                                                                      ctctTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCTTATTAATGACATTATTTTTATTAGAAGCTaa                                       >  2:81593/1‑148 (MQ=255)
                                                                                                           tGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCTTATTAATGACATTATTTTTATTAGAAGCTAACAATc                                  <  1:249363/148‑1 (MQ=255)
                                                                                                             aaaaTATATTTTACAGAATTTTATCTAAATGTTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCTTATTAATGACATTATTTTTATTAGAAGCTAACAATCtt                                >  1:626472/1‑148 (MQ=255)
                                                                                                                atatatTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCTTATTAATGACATTATTTTTATTAGAAGCTAACAATCTTGAt                             <  2:523332/148‑1 (MQ=255)
                                                                                                                atatatTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCTTATTAATGACATTATTTTTATTAGAAGCTAACAATCTTGAt                             <  2:486109/148‑1 (MQ=255)
                                                                                                                 tatatTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATAATAGTAATATTTTTTCAACCATTGTTATAGGGGGTCTTATTAATAACATTATTTTTATTAGAAGCTAACAATCTTCAtt                            >  2:554059/1‑148 (MQ=255)
                                                                                                                     ttttACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCTTATTAATGACATTATTTTTATTAGAAGCTAACAATCTTAAtttttc                        >  2:585499/1‑146 (MQ=255)
                                                                                                                                    cccaaaTATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCCTTGTTACAGGAGGTCTTACCAATCATATTATTTTTATTAGAAGCTAACAATCTTGATTTTGCATCAACGAAagaaga         <  2:575184/145‑1 (MQ=255)
                                                                                                                                     cTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCTTATTAATGACATCATTTTTATTAGAAGCTAACAATCTTGATTTTGCATCAACGAAAgaagaa        <  2:583549/148‑1 (MQ=255)
                                                                                                                                        agtatTCAAAAAAATATATTATTCAGCATCGAATGCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCTTATTAATGACATTATTTTTATTAGAAGCTAACAATCTTGATTTTGCATCAACGAAAGAAGAACTa     <  1:295955/146‑1 (MQ=255)
                                                                                                                                           atTAAAAAAAATATCTTAATATCCTATTAATCCGATAAGAATTATAGTAACATTCTTTCAACCATTGTTATAGGAGGCCTTATTAATGACATTATTATTATTAGAAGCTAACAATCTTGATTTTCCATCAACGAAAGAAGAACTAGaa  <  1:316758/148‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                
TCTTTTTGTTTCTAGCTAAACTTGCTACGACAGATTCTTTATCCATAATGATAGCCCCCTATATATATGTTTATTTACTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTTAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCTTATTAATGACATTATTTTTATTAGAAGCTAACAATCTTGATTTTGCATCAACGAAAGAAGAACTAGAA  >  CP000730/195144‑195430

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: