Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	396,540	G→A	A208T (GCA→ACA)	USA300HOU_0364 →	possible lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	396,540	0	G	A	98.4%	198.5 / NA	64	A208T (GCA→ACA)	USA300HOU_0364	possible lipoprotein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (39/24);  minor base C (1/0);  total (40/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCTGATACATTAGATATCATACCAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCGCACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATAACAACGGCGGTTATG  >  CP000730/396395‑396672                                                                                                                                                  |                                                                                                                                     aGCTGATACATTAGATATCATACCAAAATTAATGTTACAAGGTTCTGTCGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGcaca                                                                                                                                    <  2:322588/148‑1 (MQ=255)     gATACATTAGATATCATACCAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATATATTCACATACAGATTTATATGATTATAAAGCGAACGTTGAAGGCACACaaa                                                                                                                                >  2:572362/1‑148 (MQ=255)          aTTAGATATCATACCAAAATTAATGTTACAAGGTTCTGCTGACCTATTAAATGCAGTTGCAACTTCTAAAATCAAAGGTGACGAAGAAATTTATTTATATACAGATTTATATGATTTGATATTTGACGTTGAAGGCACACAAAAAAtt                                                                                                                           >  2:622704/1‑148 (MQ=255)           ttAGATATCATACCAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAAATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAAttt                                                                                                                          >  2:502641/1‑148 (MQ=255)            tAGATATCATACCAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTa                                                                                                                         >  1:504382/1‑148 (MQ=255)               atatCATACCAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGa                                                                                                                      <  1:561847/148‑1 (MQ=255)               atatCATACCAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGa                                                                                                                      >  2:495407/1‑148 (MQ=255)               atatCATACCAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAAAGTTGAAGGCACCAAAAAAATTTATGa                                                                                                                      >  2:16823/1‑148 (MQ=255)               atatCATACCAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATAAAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGa                                                                                                                      <  2:35989/148‑1 (MQ=255)                     tACCAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATAAAGACTTATATGCTCTCACAGTTAACGTAGTAATCACAATAAAATTTTATGACTTAtt                                                                                                                <  2:368058/148‑1 (MQ=255)                        cAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTaa                                                                                                             <  1:559664/148‑1 (MQ=255)                        aaaatTTAATGTTAAAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGATGGCACATAAAAAATTTATGACTTATTTaa                                                                                                             <  2:432133/147‑1 (MQ=255)                         aataTTAATGTTACCAGGTTCTGTTGACCTATTAAATGACGTTCCCACTTCTAGCATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATTTGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTaaa                                                                                                            <  2:443254/145‑1 (MQ=255)                         aaaaTTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTaaa                                                                                                            <  1:451871/148‑1 (MQ=255)                                  gTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTa                                                                                                   >  2:294952/1‑148 (MQ=255)                                  gTTAAAAGGTGCTGTTGACCTATTAAATGAAGTTGCAACTTCGAAATTCACAGGTGAAGAAGAAATTTATTCACATACATATTTACATGATTTTAAAGCGAACGTTGAATGCACACAAAAAATTTATGACTTATTTAAGCCTATTTTa                                                                                                   <  2:70131/148‑1 (MQ=255)                                     aCAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTagag                                                                                                >  2:414651/1‑148 (MQ=255)                                      cAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAGTTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTGAACCCTATTTTAaaga                                                                                               >  2:252919/1‑148 (MQ=255)                                      cAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAgaga                                                                                               >  2:153786/1‑148 (MQ=255)                                       aaGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGaa                                                                                              >  1:179151/1‑148 (MQ=255)                                        aGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGaaa                                                                                             >  2:484343/1‑148 (MQ=255)                                         ggTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGaaaa                                                                                            >  1:254036/1‑148 (MQ=255)                                                    cccaTTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATaaaaaa                                                                                 <  1:506774/145‑1 (MQ=255)                                                     cTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAAt                                                                                <  1:332845/148‑1 (MQ=255)                                                     atATTAAATGAAGTTGCAAGTTCTAAAATCACAGGTCAATAAGAAATTAATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAAGGTATGACTTATTTATACCTATTTTAGAGAAAAAAGATAAAAAAt                                                                                <  2:145724/147‑1 (MQ=255)                                                       aTTAAATGAAGTTGCAACTTCTAAAATGACAGGGGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACAAAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTa                                                                              <  1:602811/148‑1 (MQ=255)                                                       aTTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTa                                                                              >  2:564080/1‑148 (MQ=255)                                                       aTTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTa                                                                              <  2:389589/148‑1 (MQ=255)                                                           aaTGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGtg                                                                          <  1:414651/148‑1 (MQ=255)                                                           aaTGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATCTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTCTTTTAGAGAAAAAAGATAAAAAATTAAGtg                                                                          >  2:8018/1‑148 (MQ=255)                                                            aTGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGtga                                                                         >  1:320837/1‑148 (MQ=255)                                                               aaGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGtgatga                                                                      >  2:459395/1‑148 (MQ=255)                                                                 gTTGCAACTTCTAAAATCACAGGTGAAGAAGAAAGTTATTCACATACAGATTTATATGATTTTAAAGCGAAAGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGata                                                                    >  2:377622/1‑148 (MQ=255)                                                                           cTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGaa                                                          >  2:159888/1‑148 (MQ=255)                                                                             aaaaaaaaaaaagaagaagaaATTTATTCAAATACAGATTTATATGAAAATAAAGCGAACGTTAAAGCCACACAAAAAATTTATGACTTATTTCAACCTATTTTAGAAAAAAAAAAAAAAAAATTAAGTGATGATATCCAAATGAACt                                                        <  2:266110/136‑1 (MQ=255)                                                                                  cacaGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGCAGGCACACATAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAGAAAATTACGGGCTGATATCCAAATGAACTTAGAt                                                   >  1:162150/1‑148 (MQ=255)                                                                                   acaGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTAGATa                                                  >  2:183141/1‑148 (MQ=255)                                                                                      ggTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAg                                               <  1:588180/148‑1 (MQ=255)                                                                                       gTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGGTGATATCCAAATGAACTTCGATAAAGt                                              >  1:38029/1‑148 (MQ=255)                                                                                         gaagaagaaATTTATTCACATACAGATTTATATGATTTTAAAGCCAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCGAAATGAACTTCGATAAAGTGa                                            >  1:436753/1‑148 (MQ=255)                                                                                           agaagaaATTTATTCACATACAGAATTATATGATTTTAAAGCGAACATTGAAGGCACACAAAAAATTTATGACGTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAAt                                          <  1:142814/148‑1 (MQ=255)                                                                                              agaaATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATGTCCAAATGAACTTCGATAAAGTGAATCaa                                       >  1:519305/1‑148 (MQ=255)                                                                                              agaaATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCaa                                       <  1:319855/148‑1 (MQ=255)                                                                                              agaaATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCaa                                       >  2:581315/1‑148 (MQ=255)                                                                                               gaatataatgcagaTACAGACTTACATGATTTCAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAat                                      <  1:615853/135‑1 (MQ=255)                                                                                               gaaATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAat                                      >  2:138588/1‑148 (MQ=255)                                                                                                aaaaTTATTCACAAACAGATTTATATGATGTTAAAGCGAACGTTGAAAGAAAAAAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAatt                                     <  2:399474/148‑1 (MQ=255)                                                                                                aaaTTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAGGTGTTGCTTTACAAATGAACTTCGATAAAGTGAATCAatt                                     >  1:238587/1‑148 (MQ=255)                                                                                                         cacaTACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATaa                            <  1:212860/148‑1 (MQ=255)                                                                                                             tACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAatat                        >  2:249032/1‑148 (MQ=255)                                                                                                             tACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAatat                        >  2:249301/1‑148 (MQ=255)                                                                                                             tACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAatat                        <  1:502641/148‑1 (MQ=255)                                                                                                              aCAGATTTATATGATTTTAAAACGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAAtata                       <  1:217047/148‑1 (MQ=255)                                                                                                                 ggatcaaatGATTTTAAAGGCAAGAGCGAAGGCCCTCAAACACTTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGACGATATCCAAATGAACTTCGATGAAGTGAATCAATTATTGGATAAAAATAAAg                    >  1:465347/8‑148 (MQ=255)                                                                                                                        atGATTTTAAAGCGAACGTTGAAGGCACGCAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTAGAAATAGTAAGACAATTATTGGATAAATATAAAGATaacaa             >  1:389275/1‑148 (MQ=255)                                                                                                                        atGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATaacaa             <  1:89929/148‑1 (MQ=255)                                                                                                                          gATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAACAAGATGCAAAATTAAGTGATGATATCCAAATGAACTTCGGTAAAGTGAATCAATTATTGGATAAATATAAAGATAACAAcg           >  1:33845/1‑148 (MQ=255)                                                                                                                          gATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATAACAAcg           >  2:168347/1‑148 (MQ=255)                                                                                                                           aTTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGCTGATATCCAAATGAACTTCGATCAAGTGAATCAATTATTGGATAAATATAAAGATAACAAcgg          >  2:456156/1‑148 (MQ=255)                                                                                                                           aTTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAGGATAACAAcgg          >  1:126438/1‑148 (MQ=255)                                                                                                                           aTTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATAACAAcgg          >  1:41895/1‑148 (MQ=255)                                                                                                                           aTTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATAACAAcgg          >  1:245582/1‑148 (MQ=255)                                                                                                                                  aGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATAACAACGGCGGTTAt   >  1:218789/1‑148 (MQ=255)                                                                                                                                  aGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATAACAACGGCGGTTAt   >  1:344557/1‑148 (MQ=255)                                                                                                                                   gCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATAAATTATTGGATAAATATAAAGATAACAACGGCGGTTATg  >  1:572955/1‑148 (MQ=255)                                                                                                                                                  |                                                                                                                                     AGCTGATACATTAGATATCATACCAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCGCACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATAACAACGGCGGTTATG  >  CP000730/396395‑396672

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑