Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	629,681	C→A	L84I (CTA→ATA)	hxlA →	possible 3‑hexulose‑6‑phosphate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	629,681	0	C	A	100.0%	154.7 / NA	49	L84I (CTA→ATA)	hxlA	possible 3‑hexulose‑6‑phosphate synthase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (24/25);  total (24/25)

ACAACGAAGGTTTACCAGCAGTTAAACATATGGCAGACAACATTAGTAATGTAAAAGTATTAGCAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATACTAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTGCTGTTCAAGATTTAGAAAAACGTGCAAAAGAACTAGATGAAATGGGTGCTGATTATAT  >  CP000730/629541‑629823                                                                                                                                             |                                                                                                                                               aCAACGAAGGTTTACCAGCAGTTAAACATATGGCAGACAACATTAGTAATGTAAAAGTATTAGCAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGgtgt                                                                                                                                         >  2:95042/1‑148 (MQ=255)  cAACGAAGGTTTACCAGCAGTTAAACATATGGCAGACAACATTAGTAATGTAAAAGTATTAGCAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGtt                                                                                                                                        >  2:22159/1‑148 (MQ=255)  cAACGAAGGTTTACCAGCAGTTAAACATATGGCAGACAACATTAGTAATGTAAAAGTATTAGCAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTCtt                                                                                                                                        >  2:308879/1‑148 (MQ=255)  cAACGAAGGTTTACCAGCAGTTAAACATAAGGCAGACAACATTAGTTATGTAAAAGTATTAGAAGACATGAAAATTATAAATGCAGCTGATTATGAAGTTAGCCAAACAATTAAATTCGGCGCGGATGTAAGTAAAATAATAAgtgtg                                                                                                                                        >  2:590788/1‑147 (MQ=255)          gattAACAGCAGTTAAACATATGGCAGACAACATTAGTAATGTAAAATTATTAACAGACATTAAAATTATAGATGCAGTTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCagaaga                                                                                                                                <  1:474689/146‑1 (MQ=255)               ccAGCAGTTAAACATATGGCAGACAACATTAGTAATGTAAAAGTATTAGCAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGcgggggaagcaa                                                                                                                           >  2:600804/1‑137 (MQ=255)                          aCATATGGCAGACAACATTAGTAATGTAAAAGTATTAGCAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAagca                                                                                                                <  1:25788/148‑1 (MQ=255)                           cATATGGCAGACAACATTAGTAATGTAAAAGTATTAGCAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCTGAAGATGCATCAATTAAagcag                                                                                                               <  1:337218/148‑1 (MQ=255)                               tGGCAGACAACATTAGTAATGTAAAAGTATTAGCAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGCTGTAATTACAATAATAGGTGTTGCAGACGATGCATCAATTAAAGCAGCTAt                                                                                                           >  1:212537/1‑148 (MQ=255)                                ggCAGACAACATTAGTAATGTAAAAGTATTAGCAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAGGCAGCTAtt                                                                                                          <  1:445913/148‑1 (MQ=255)                                       aacaTTAGTAATGTAAAAGTATTAGCAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGaagaag                                                                                                   <  1:189510/148‑1 (MQ=255)                                                      aaaGTATTAGCAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAataata                                                                                    >  2:292558/1‑148 (MQ=255)                                                      aaaGTATTAGCAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCCCATAAAAataata                                                                                    >  2:355464/1‑148 (MQ=255)                                                          tATTAGCAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACa                                                                                >  1:280070/1‑148 (MQ=255)                                                          tATTAGCAGACATGAAAAGTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACa                                                                                >  2:545769/1‑148 (MQ=255)                                                            ttAGCAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGAAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAAt                                                                              <  1:218557/148‑1 (MQ=255)                                                              aGCAGACATGAAAATTATAGCTGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTa                                                                            <  2:16439/148‑1 (MQ=255)                                                              aGCAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTa                                                                            <  2:462223/148‑1 (MQ=255)                                                               gCAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTAc                                                                           >  2:448048/1‑148 (MQ=255)                                                               gCAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCGCATAAAAATAATAAACAATTAc                                                                           >  2:210095/1‑148 (MQ=255)                                                               gCAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAACTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTAc                                                                           >  1:431126/1‑148 (MQ=255)                                                                cAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACt                                                                          >  1:348203/1‑148 (MQ=255)                                                                cAGACATGAAAATTATAGATGCAGCCGACCATGAATTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATACAAATAATAAACAATTACt                                                                          >  1:305402/1‑148 (MQ=255)                                                                    cATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGtt                                                                      <  1:95042/148‑1 (MQ=255)                                                                                       gCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAATGCAGCTATTGACGAAGATCGTAAAAATAATAAACAATTACTAGTTGATATGATTGCTGTTCAAg                                                   <  1:302120/148‑1 (MQ=255)                                                                                         tGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTGCTGTTCAAGAt                                                 <  1:283628/148‑1 (MQ=255)                                                                                          gATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTGCTGTTCAAGAtc                                                <  2:581396/148‑2 (MQ=255)                                                                                            ttATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTGCTGTTCAAGATTTa                                              <  1:363761/148‑1 (MQ=255)                                                                                              aTGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTGCTGTTCAAGATTTAGa                                            <  1:415757/148‑1 (MQ=255)                                                                                               tGAAGTTAGCCAAGCAATTACATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGCAACTGCATCAATTAAAGCAGCTATTGGAGAAGCACATAAAAATAATAAACAATTACTAGTTGATATGATTGCTGGTCAAGATTTaaaa                                           >  2:279629/1‑148 (MQ=255)                                                                                                    ttAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTGCTGTTCAAGATTTAGAAAAACg                                      <  1:360867/148‑1 (MQ=255)                                                                                                     tAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTGCTGTTCAAGATTTAGAAAAACGt                                     >  2:547047/1‑148 (MQ=255)                                                                                                     tAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTGCTGTTCAAGATTTAGAAAAACGt                                     >  1:302891/1‑148 (MQ=255)                                                                                                       gCCAGGCAATTAAATTTGGCGCGGATGTAATTACAATAATCGGAGTTGCACACGTTGCATCAATTAAAGCAGCTATTCAAGTAGCTCATAAAAATAATTAATCATTACTAGTTGATATGATTGCTGTTCACGATTTAGAAAAACGTGc                                   <  2:168604/148‑1 (MQ=255)                                                                                                           aGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTGCTGTTCAAGATTTAGAAAAACGTGCaaaa                               >  1:568104/1‑148 (MQ=255)                                                                                                              aaTTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTGCTAGTTGATATGATTGCGGTGCAAGATTTAGAAAAACGTGCAAAAGaa                            <  2:212537/148‑1 (MQ=255)                                                                                                              aaTTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTGCTGTTCAAGATTTAGAAAAACGTGCAAAAGaa                            <  1:132882/148‑1 (MQ=255)                                                                                                              aaTTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTGCTGTTCAAGATTTAGAAAAACGTGCAAAAGaa                            <  1:22159/148‑1 (MQ=255)                                                                                                                ttAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTGCTGTTCAAGATTTAGAAAAACGTGCAAAAGAACt                          <  1:69914/148‑1 (MQ=255)                                                                                                                          cgcgGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATGACTAGTTGATATGATTGCTGTTCAAGATTTAGAAAAACGTGCAAAAGAACTAGATGAAATg                <  1:621630/148‑1 (MQ=255)                                                                                                                            cgGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAATCAGCTATTGAAGGAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTGCTGTTCAAGATTTAGAAAAACGTGCAAAAGAACTAGATGAAATggg              >  1:148332/1‑148 (MQ=255)                                                                                                                                    aTTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTGCTGTTCAAGATTTAGAAAAACGTGCAAAAGAACTAGATGTACTGGGTTCTGAtt      <  2:545637/148‑1 (MQ=255)                                                                                                                                    aTTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTGCTGTTCAAGATTTAGAAAAACGTGCAAAAGAACTAGATGAAATGGGTGCTGAtt      >  1:495549/1‑148 (MQ=255)                                                                                                                                    aTTACAATAATAGGTGTTGCAGAAAATGAATCAATTAAAGCAGCAATTGAAGAAGCTCATAAAAATAATAAACAATTACGAGTTGATATGATTGCTGTTCAAGATTTAGAAAAACGTGCAAAAGAACTAGATGAAATGGGTGCTGAtt      <  2:130876/148‑1 (MQ=255)                                                                                                                                     ttACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTGCTGTTCAAGATTTAGAAAAACGTGCAAAAGAACTAGATGAAATGGGTGCTGATta     <  1:292558/148‑1 (MQ=255)                                                                                                                                     ttACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTGCTGTTCAAGATTTAGAAAAACGTGCAAAAGAACTAGATGAAATGGGTGCTGATta     <  1:308879/148‑1 (MQ=255)                                                                                                                                      tACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCCGATAGTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTGCTGTTCAAGATTTAGAAAAACGTGCAAAAGAACTAGATGAAATGGGGGCTGATtat    >  2:576489/1‑148 (MQ=255)                                                                                                                                      tACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTGCTGTTCAAGATTTAGAAAAACGTGCAAAAGAACTAGATGAAATGAGTGCTGATtat    >  2:301723/1‑148 (MQ=255)                                                                                                                                        cAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTGCTGTTCAAGATTTAGAAAAACGTGCAAAAGAACTAGATGAAATGGGTGCTGGTtatag  >  1:44846/1‑147 (MQ=255)                                                                                                                                             |                                                                                                                                               ACAACGAAGGTTTACCAGCAGTTAAACATATGGCAGACAACATTAGTAATGTAAAAGTATTAGCAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATACTAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTGCTGTTCAAGATTTAGAAAAACGTGCAAAAGAACTAGATGAAATGGGTGCTGATTATAT  >  CP000730/629541‑629823

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑