Predicted mutation
evidence seq id position mutation annotation gene description
RA CP000730 665,392 T→G L79* (TTA→TGA)  iolS → possible acetytransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730665,3920TG98.5% 217.0 / NA 67L79* (TTA→TGA) iolSpossible acetytransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base G (32/34);  minor base A (0/1);  total (32/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TACGCTGCGACAACAGTTAATCACTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACTTAGAAGTAGATAATAAAAATGAAGAAGCAGT  >  CP000730/665247‑665533
                                                                                                                                                 |                                                                                                                                             
taCGCTGCGACAACAGTTAATCACTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATgat                                                                                                                                             >  2:68722/1‑148 (MQ=255)
 aCGCTGCGACAACAGTTAATCACTTGTTAGAAGCTTCATATAATGAACATCCTTGAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTGGCTAACTTTATCTACGGGGAAGAATgata                                                                                                                                            >  2:556464/1‑148 (MQ=255)
 aCGCTGCGACAACAGTTAATCACTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATgata                                                                                                                                            >  1:238020/1‑148 (MQ=255)
 aCGCTGCGACAACAGTTAATCACTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGACCTTCATGGATGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATgata                                                                                                                                            >  1:298500/1‑148 (MQ=255)
  cGCTGCGACAACAGTTAATCACTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGatat                                                                                                                                           >  1:436404/1‑148 (MQ=255)
   gCTGCGACAACAGTTAATCACTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATAtt                                                                                                                                          >  1:107109/1‑148 (MQ=255)
   gCTGCGACAACAGTTAATCACTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATAtt                                                                                                                                          >  1:400377/1‑148 (MQ=255)
   gCTGCGACAACAGTTAATCACTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATAtt                                                                                                                                          >  1:110432/1‑148 (MQ=255)
   gCTGCGACAACAGTTAATCACTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATCTACGGTGAAGAATGATAtt                                                                                                                                          >  2:583217/1‑148 (MQ=255)
    gtGCGACAACAGTGAATCAATTGTTAGAAGCTTCATATAATGAATATCATTTACAGAAAAGACTTCAAGAACACTTATTCTGAGTAGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATAttt                                                                                                                                         <  2:105150/147‑1 (MQ=255)
    cTGCGACAACAGTTAATCACTTGTTAGAAGATTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAGTTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGGATGATAtta                                                                                                                                         >  1:483318/1‑147 (MQ=255)
     tGCGACAACAGTTAATCACTTGTTAGAAGCTTCATATCATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTa                                                                                                                                        >  1:89485/1‑148 (MQ=255)
       cGACAACAATTAATCACTTCTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATc                                                                                                                                      <  2:331798/148‑1 (MQ=255)
           aactGTTTATCACTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCt                                                                                                                                  <  2:431673/148‑1 (MQ=255)
            acaGTTAATCACTTGTTAGAAGCTTCAGATAATGAACATCATTTACAGAAAAGTCGTCAAGAACAATTATTCTAAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTc                                                                                                                                 <  2:157440/148‑1 (MQ=255)
            acaGTTAATCACTTGTGAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACGTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTc                                                                                                                                 <  1:583660/148‑1 (MQ=255)
                  aaTCACTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATg                                                                                                                           <  1:37858/148‑1 (MQ=255)
                  aaTCACTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCCTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATg                                                                                                                           <  1:449665/148‑1 (MQ=255)
                       cTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTaaa                                                                                                                      <  1:538715/148‑1 (MQ=255)
                           ttAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAAGGATATTTATCAGCTCATTATGTTAACCCAg                                                                                                                  >  2:83828/1‑148 (MQ=255)
                            tAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGa                                                                                                                 >  2:76680/1‑148 (MQ=255)
                             aGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTTGCTTTGCTAACTTTATTTACGGTGACGAATGATCTTTATCAGCTCATTATGTTAAACCAGaa                                                                                                                <  1:407817/148‑1 (MQ=255)
                               aaGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATc                                                                                                              >  2:108061/1‑148 (MQ=255)
                               aaGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATc                                                                                                              >  2:108049/1‑148 (MQ=255)
                                aGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCg                                                                                                             <  2:436404/148‑1 (MQ=255)
                                  cTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCa                                                                                                           <  1:254787/148‑1 (MQ=255)
                                     cATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACa                                                                                                        >  2:172649/1‑148 (MQ=255)
                                     cATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACa                                                                                                        >  1:446800/1‑148 (MQ=255)
                                       tataATAAACATCATTTAAAGAAAAGACTTCAAGAACTATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATa                                                                                                      <  1:19521/148‑1 (MQ=255)
                                         taataaACATCTTTTAAAAAAAAGCCTTCAAGAACAATTATTCTTCTTCGTTGAAGAAGGTAAGGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACa                                                                                                    <  1:290625/148‑1 (MQ=255)
                                           aTGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAgg                                                                                                  >  2:376144/1‑148 (MQ=255)
                                           aTGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCAAAAAATACAga                                                                                                  >  2:119601/1‑147 (MQ=255)
                                            tGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGt                                                                                                 <  2:238020/148‑1 (MQ=255)
                                               aCATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTAt                                                                                              >  1:58875/1‑148 (MQ=255)
                                                    atTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTAc                                                                                         <  2:88043/148‑1 (MQ=255)
                                                          agaaaagaCTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCAtt                                                                                   >  2:105517/1‑148 (MQ=255)
                                                            aaaagaCTTCAAGAAAAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGt                                                                                 >  2:591499/1‑148 (MQ=255)
                                                             gaagtCTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAGCTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACCGGATAAGGTACAGCATTGtt                                                                                >  2:109504/2‑148 (MQ=255)
                                                              aagaCTTCAAGAACAATTATTCGTAGTCGATGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGAGATTTATCAGCTCATTATGTTAAACCAGAATCGCACGATAAAGGTTAAGGAACAACATTGGTa                                                                               >  2:432111/1‑148 (MQ=255)
                                                               agaCTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAAATTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTaa                                                                              >  1:419383/1‑148 (MQ=255)
                                                                         aaCAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTCACTTTATTTACGGTGAAGAATGATATTTATAAGCTCATTATGTTAAACCAGAGTCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAgggat                                                                    >  2:263543/1‑145 (MQ=255)
                                                                         aaCAATTATTCTTAGTCGTTGAAAAAGGTGAGGACATGGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATATAGGTTATGGTACAGCATTGTTAAATGACGGaat                                                                    >  2:446978/1‑146 (MQ=255)
                                                                             attattCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCa                                                                >  1:330236/1‑148 (MQ=255)
                                                                                      aGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTGTTACGGTTGGaa                                                       <  2:545607/148‑1 (MQ=255)
                                                                                         cGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGAt                                                    <  2:250278/148‑1 (MQ=255)
                                                                                                gaagGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTg                                             <  2:219065/148‑1 (MQ=255)
                                                                                                gaagGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTg                                             <  1:329944/148‑1 (MQ=255)
                                                                                                          aCATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGGTATGGTACAGCATNGTTAAATGAAGGATGATCACGNTNTGAAGNTNAATTTGAAGGCGTTTa                                   >  1:630383/1‑148 (MQ=255)
                                                                                                          aCATCGTTGGCCTTGCTAACTTTATTTACGGCGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTTTTTa                                   <  2:441567/148‑1 (MQ=255)
                                                                                                            aTCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACt                                 <  2:322772/148‑1 (MQ=255)
                                                                                                             acGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAAGGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACtt                                <  2:87209/147‑1 (MQ=255)
                                                                                                               gTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACTTAg                              <  2:613462/148‑1 (MQ=255)
                                                                                                               gTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACTTAg                              <  2:369368/148‑1 (MQ=255)
                                                                                                               cttcgCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACTTAg                              <  2:600616/144‑1 (MQ=255)
                                                                                                                 ttgCTTTGGTAACTTTTTTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAACTGAAGGACTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACTTAGaa                            <  1:278451/146‑1 (MQ=255)
                                                                                                                      ttGCTAACTTTATTTAAGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTAAAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACTTAGAAGTAGa                       <  2:50754/148‑1 (MQ=255)
                                                                                                                       tGCTAACTTTATTGACGTGGAAGAATAATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTACATGAGGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACTTAGAAGTAGat                      <  2:226670/148‑1 (MQ=255)
                                                                                                                         cTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACTTAGAAGTAGataa                    <  1:552477/148‑1 (MQ=255)
                                                                                                                           aaCTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTAATTAGAAGTAGataata                  >  2:39405/1‑148 (MQ=255)
                                                                                                                            aCTTTATTTACGGTGAAGAATGAAATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACTTAGAAGTAGAtaataa                 <  2:550202/148‑1 (MQ=255)
                                                                                                                             cTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACTTAGAAGTAGATAATaaa                >  1:34245/1‑148 (MQ=255)
                                                                                                                               ttatttaCGGTGAAGAATGATATTTTTCAGCTCATTATGTTAAACCAGAAACGCAAAATACAGGTTATGGTACAGCATTGTTAAATGAAGTATTATCCCGTTTTGAAGATAAAGTTGTAGGTGTTTACTTAGAAAAAGATaaaaaaaa              <  2:526630/148‑1 (MQ=255)
                                                                                                                               ttatttaCGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACTTAGAAGTAGATAATaaaaa              >  2:415146/1‑148 (MQ=255)
                                                                                                                                     aCGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAAGCTATAGGTTATGGTTATGGAATGTGAGATGAAGGAGAAACAGGTTTTGAATTTAGATCTGTGGGTTTTTATTTAGCAGGAGATAATAAAATTgaaga        >  2:622789/1‑148 (MQ=255)
                                                                                                                                      cGGTGAAGAATGATATTTATCAGCTCATTCTGTTATGCGAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACTTAGAAGTGGATAATAAAAATgaagaa       >  2:288880/1‑148 (MQ=255)
                                                                                                                                      cGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACTTAGAAGTAGATAATAAAAATgaagaa       <  2:419383/148‑1 (MQ=255)
                                                                                                                                       ggTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACTTAGAAGTAGATAATAAAAATGaagaag      <  2:31430/148‑1 (MQ=255)
                                                                                                                                       cgTGAAGAATGATTTTTTACAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGTTTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACTTAGAAGTAGATAATAAAAATGaagaag      <  2:587616/147‑1 (MQ=255)
                                                                                                                                          gaagaaTGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACTTAGAAGTAGATAATAAAAATGAAGAAGCAg   <  1:481089/148‑1 (MQ=255)
                                                                                                                                          gaagaaTGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAAAGAAGGATTATCACGATTTGAAGATAAACTTGAAGGTGTTTCACTAGAAGTAGATAAGAAAAATGAAGAAGCAg   >  2:273180/1‑148 (MQ=255)
                                                                                                                                           aagaaTGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACTTAGAAGTAGATAATAAAAATGAAGAAGCAGt  >  2:491016/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                             
TACGCTGCGACAACAGTTAATCACTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACTTAGAAGTAGATAATAAAAATGAAGAAGCAGT  >  CP000730/665247‑665533

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑