BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	456,707	T→A	6.1%	S88R (AGT→AGA)	set25 →	staphylococcal exotoxin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	456,707	0	T	A	6.1%	290.5 / 6.1	104	S88R (AGT→AGA)	set25	staphylococcal exotoxin
Reads supporting (aligned to +/- strand): ref base T (55/42); new base A (3/4); total (58/46)
Fisher's exact test for biased strand distribution p-value = 6.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.47e-01

Only 100 of 104 total aligned reads displayed.

AAGCATTATACCGATACTACACTGGAAAGACTATGGAAATGAAAAATATTAGTGCTTTGAAACATGGTAAAAACAACTTACGTTTTAAGTTTAGAGGTATTAAGATTCAAGTTTTACTGCCTGGAAATGATAAAAGTAAATTTCAACAGCGTAGTTATGAGGGGTTAGATGTTTTCTTTGTTCAAGAAAAAAGAGATAAGCACGATATATTTTATACTGTTGGTGGTGTAATACAGAATAATAAAACATCTGGAGTTGTCAGTGCACCAATATT  >  CP000730/456571‑456844

aaGCATTATACCGATACTACACTGGAAAGACTATGGAAATGAAAAATATTAGTGCTTTGAAACATGGTAAAAACAACTTACGTTTTAAGTTTAGAGGTATTAAGATTCAAGTTTTACTGCCTGGAAATGATAAAAGTAAAt                                                                                                                                       <  1:67193/141‑1 (MQ=255)

 aGCATTATACCGATACTACACTGGAAAGACTATGGAAATGAAAAATATTAGTGCTTTGAAACATGGTAAAAACAACTTACGTTTTAAGTTTAGAGGTATTAAGATTCAAGTTTTACTGCCTGGAAATGATAAAAGTAAAtt                                                                                                                                      <  2:52171/141‑1 (MQ=255)

      tataCCGATACTACACTGGAAAGACTATGGAAATGAAAAATATTAGTGCTTTGAAACATGGTAAAAACAACTTACGTTTTAAGTTTAGAGGTATTAAGATTCAAGTTTTACTGCCTGGAAATGATAAAAGTAAATTTCAAc                                                                                                                                 <  2:324081/141‑1 (MQ=255)

      tataCCGATACTACACTGGAAAGACTATGGAAATGAAAAATATTAGTGCTTTGAAACATGGTAAAAACAACTTACGTTTTAAGTTTAGAGGTATTAAGATTCAAGTTTTACTGCCTGGAAATGATAAAAGTAAATTTCAAc                                                                                                                                 <  1:703121/141‑1 (MQ=255)

      tataCCGATACGACACTGGAAAGACTATGGAAATGAAAAATATTAGTGCTTTGAAACATGGTAAAAACAACTTACGTTTTAAGTTTAGAGGTATTAAGATTCAAGTTTTACTGCCTGGAAATGATAAAAGTAAATTTCAAc                                                                                                                                 <  2:267282/141‑1 (MQ=255)

       ataCCGATACTACACTGGAAAGACTATGGAAATGAAAAATATTAGTGCTTTGAAACATGGTAAAAACAACTTACGTTTTAAGTTTAGAGGAAGTAAGATTCAAGTTTTACTGCCTGGAAATGAGAAAAGTAAATGTCAACa                                                                                                                                >  2:709525/1‑141 (MQ=255)

        taCCGATACTACACTGGAAAGACTATGGAAATGAAAAATATTAGTGCTTTGAAACATGGTATAAACAACTTACGTTTTAAGTTTAGCGGTATTAAGATTCAAGTTTTACTGCCTGGAAATTATAAAAGTAAATTTCAACAg                                                                                                                               <  1:523960/141‑1 (MQ=255)

        taCCGATACTACACTGGAAAGACTATGGAAATGAAAAATATTAGTGCTTTGAAACATGGTAAAAACAACTTACGTTTTAAGTTTAGAGGTATAAAGATTCAAGTATTACTGCATGGAAATAATAAAAATAAATTTAAACAg                                                                                                                               >  2:424034/1‑141 (MQ=255)

        aaacGATACTACACTTGAAAGACTATGGAAATGAAAAATATTAGTGCTTTGAAACATGGTAAAAACAACTTACGTTTTAAGTTTAGAGGTATTAAGATTCAAGTTTTACTGCCTGGAAATGATAAAAGTAAATTTCAACAg                                                                                                                               <  2:580630/138‑1 (MQ=255)

        aaCCGATACTACACTGGAAAGACTATGGAAATGAAAAATATTAGTGCTTTGAAACATGGTAAAAACAACTTACGTTTTAAGTTTAGAGGTATTAAGATTCAAGTTTTACTGCCTGGAAATGATAAAAGTAAATTTCAACAg                                                                                                                               <  2:301430/140‑1 (MQ=255)

            gATACTACACTGGAAAGACTATGGAAATGAAAAATATTAGTGCTTTGAAACATGGTAAAAACAACTTACGTTTTAAATTTAGAGGTATTAAGATTCAAATTTTACTGCCTGGAAATGATAAAAGTAAATTTCAAAAGCGTa                                                                                                                           >  1:332368/1‑141 (MQ=255)

                    acTGGAAAGACTATGGAAATGAAAAATATTAGTGCTTTGAAACATTGTAAAAACAACTTACGTTTTAAGTTTAGAGGTATTAAGATTCAAGTTTTACTGCCTGGAAATGATAAAAGTAAATTTCAACAGCGTAGTTATGAg                                                                                                                   <  2:62724/141‑1 (MQ=255)

                    acTGGAAAGACTATGGAAATGAAAAATATTAGTGCTTTGAAACATGGTAAAAACAACTTACGTTTTAAGTTTAGAGGTATTAAGATTCAAGTTTTACTGCCTGGAAATGATAAAAGTAAATTTCAACAGCGTAGTTATGAg                                                                                                                   <  1:648127/141‑1 (MQ=255)

                     cTGGAAAGACTATGGAAATGAAAAATATTAGTGCTTTGAAACATGGTAAAAACAACTTACGTTTTAAGTTTAGAGGTATTAAGATTCAAGTTTTACTGCCTGGAAATGATAAAAGTAAATTTCAACAGCGTAGTTATGAgg                                                                                                                  >  2:415115/1‑141 (MQ=255)

                      tGGAAAGACTATGGAAATGAAAAATATTAGTGCTTTGAAACATGGTAAAAACAACTTACGTTTTAAGTTTAGAGGTATTAAGATTCAAGTTTTACTGCCTGGAAATGATAAAAGTAAATTTCAACAGCGTAGTTATGAggg                                                                                                                 <  1:30236/141‑1 (MQ=255)

                      tGGAAAGACTATGGAAATGAAAAATATTAGTGCTTTGAAACATGGTAAAAACAACTTACGTTTTAAGTTTAGAGGTATTAAGATTCAAGTTTTACTGCCTGGAAATGATAAAAGTAAATTTCAACAGCGTAGTTATGAggg                                                                                                                 <  1:65183/141‑1 (MQ=255)

                        gAAAGACTATGGAAATGAAAAATATTAGTGCTTTGAAACATGGTAAAAACAACTTACGTTTTAAGTTTAGAGGTATTAAGATTCAAGTTTTACTGCCTGGAAATGATAAAAGTAAATTTCAACAGCGTAGTTATGAGGGGt                                                                                                               >  2:108133/1‑141 (MQ=255)

                           aGACTATGGAAATGAAAAATATTAGTGCTTTGAAACATGGTAAAAACAACTTACGTTTTAAGTTTAGAGGTATTAAGATTCAAGTTTTACTGCCTGGAAATGATAAAAGTAAATTTCAACAGCGTAGTTATGAGGGGTTAg                                                                                                            >  1:674766/1‑141 (MQ=255)

                            gACTATGGAAATGAAAAATATTAGTGCTTTGAAACATGGTAAAAACAACTTACGTTTTAAGTTTAGAGGTATTAAGATTCAAGTTTTACTGCCTGGAAATGATAAAAGTAAATTTCAACAGCGTAGTTATGAGGGGTTAGa                                                                                                           >  2:742668/1‑141 (MQ=255)

                            gACTATGGAAATGAAAAATATTAGTGCTTTGAAACATGGTAAAAACAACTTACGTTTTAAGTTTAGAGGTATTAAGATTCAAGTTTTACTGCCTGGAAATGATAAAAGTAAATTTCAACAGCGTAGTTATGAGGGGTTAGa                                                                                                           >  2:412992/1‑141 (MQ=255)

                            gACTATGGAAATGAAAAATATTAGTGCTTTGAAACATGGTAAAAACAACTTACGTTTTAAGTTTAGAGGTATTAAGATTCAAGTTTTACTGCCTGGAAATGATAAAAGTAAATTTCAACAGCGTAGATATGAGGTGGTAGa                                                                                                           >  2:47575/1‑141 (MQ=255)

                            gACTATGGAAATGAAAAATATTAGTGCTTTGAAACATGGTAAAAACAACTTACGTTTTAAGTTTAGAGGTATTAAGATTCAAGTTTTACTGCCTGGAAATGATAAAAGTAAATTTCAACACAGTAGTTATGAGGGGTTAGa                                                                                                           >  1:370066/1‑141 (MQ=255)

                             aCTATGGAAATGAAAAATATTAGTGCTTTGAAACATGGTAAAAACAACTTACGTTTTAAGTTTAGAGGTATTAAGATTCAAGTTTTACTGCCTGGAAATGATAAAAGTAAATTTCAACAGCGTAGTTATGAGGGGTTAGAt                                                                                                          >  2:42748/1‑141 (MQ=255)

                               tATGGAAATGAAAAATATTAGTGCTTTGAAACATGGTAAAAACAACTTACGTTTTAAGTTTAGAGGTATTAAGATTCAAGTTTTACTGCCTGGAAATGATAAAAGTAAATTTCAACAGCGTAGTTTTGAGGGGTTAGATGt                                                                                                        >  2:388284/1‑141 (MQ=255)

                               tATGGAAATGAAAAATATTAGTGCTTTGAAACATGGTAAAAACAACTTACGTTTTAAGTTTAGAGGTATTAAGATTCAAGTTTTACTGCCTGGAAATGATAAAAGTAAATTTCAACAGCGTAGTTATGAGGGGTTAGATGt                                                                                                        >  2:426868/1‑141 (MQ=255)

                               tATGGAAATGAAAAATATTAGTGCTTTGAAACATGGTAAAAACAACTTACGTTTTAAGTTTAGAGGTATTAAGATTCAAGTTTTACTGCCTGGAAATGATAAAAGTAAATTTCAACAGCGTAGGTATGAGGGGTTAGATGt                                                                                                        >  2:106148/1‑141 (MQ=255)

                                 tGGAAATGAAAAATATTAGTGCTTTGAAACATGGTAAAAACAACTTACGTTTTAAGTTTAGAGGTATTAAGATTCAAGTTTTACTGCCTGGAAATGATAAAAGTAAATTTCAACAGCGTAGTTATGAGGGGTTAGATGttt                                                                                                      >  1:440754/1‑141 (MQ=255)

                                 tGGAAATGAAAAATATTAGTGCTTTGAAACATGGTAAAAACAACTTACGTTTTAAGTTTAGAGGTATTAAGATTCAAGTTTTACTGCCTGGAAATGATAAAAGTAAATTTCAACAGCGTAGTTATGAGGGGTTAGATGttt                                                                                                      >  1:45198/1‑141 (MQ=255)

                                 tGGAAATGAAAAATATTAGTGCTTTGAAACATGGTAAAAACAACTTACGTTTTAAGTTTAGAGGTATTAAGATTCAAGTTTTACTGCCTGGAAATGATAAAAGTAAATTTCAACAGCGTAGTTATGAGGGGTTAGATGttt                                                                                                      >  1:640996/1‑141 (MQ=255)

                                 tGGAAATGAAAAATATTAGTGCTTTGAAACATGGTAAAAACAACTTACGTTTTAAGTTTAGAGGTATTAAGATTCAAGTTTTACTGCCTGGAAATGATAAAAGTAAATTTCAACAGCGCAGTTATGAGGGGTTAGATGttt                                                                                                      >  2:101606/1‑141 (MQ=255)

                                 tGGAAATGAAAAATATTAGTGCTATGATACAAGTGAAAAACAACTTACGTTTTAAGTTTAGAGGTATTAAGATTCAAGTTTTACTGCCTGGAAATGATAAAAGTAAATTTCAACAGCGTAGTTATGAGGGGTTAGATGatt                                                                                                      >  1:392730/1‑141 (MQ=255)

                                 tGGAAATGAAAAATATGAGCGCTTTGAAACATGGTAAACACAACTTACGTTTTAAGTTTAGAGGTATTAAGATTCAAGTTTTACTGGCTGGAAATGATAAAAGTAAATTTCAACAGCGTAGTTATGAGGGGTTAGATGttt                                                                                                      >  1:452910/1‑141 (MQ=255)

                                 gggAAAAGAAAAATATGAGTGCTTTGAAACATGGTGAGAACAACTTACGTGTAAATTTAAGAGGTATTAAGATTCAAGTTTTACTGCCTGGAAATGATAAAAGTAAATTTCAACAGCGTAGTTATGGGGGGTTAGACGttt                                                                                                      <  2:567882/140‑1 (MQ=255)

                                  ggAAATGAAAAATATTAGTGCTTTGAAACATGGTAAAAACAACTTACGTTTTAAGTTTAGAGGTATTAAGATTCAAGTTTTACTGCCTGGAAATGATAAAAGTAAATTTCAACAGCGTAGTTATGAGGGGTTAGATGtttt                                                                                                     <  1:684564/141‑1 (MQ=255)

                                        gAAAAATATTAGTGCTTTGAAACATGGTAAAAACAACTTACGTTTTAAGTTTAGAGGTATTAAGATTCAAGTTTTACTGCCTGGAACGGATAAAAGTAAATTTCAACAACGTAGGTATGAGAGGATAGATGTTTTCTTTGt                                                                                               >  1:771545/1‑141 (MQ=255)

                                        gAAAAATATTAGTGCTTTGAAACATGGTAAAAACAACTTACGTTTTAAGTTTAGAGGTATTAAGATTCAAGTTTTACTGCCTGGAAATGATAAAAGTAAATTTCAACAGCGTAGTTATGAGGGGTTAGATGTTTTCTTTGt                                                                                               >  2:344327/1‑141 (MQ=255)

                                          aaaaTATTAGTGCTTTGAAACATGGTACAAACAACTTACGTTTTAAGTTTAGAGGTATTAAGATTCAAGTTTTACTGCCTGGAAATGATAAAAGTAAATTTCAACAGCGTAGTTATGAGGGGTTAGATGTTTTCTTTGTTc                                                                                             <  1:299914/141‑1 (MQ=255)

                                          aaaaTATTAGTGCTTTGAAACATGGTAAAAACAACTTACGTTTTAAGTTTAGAGGTATTAAGATTCAAGTTTTACTGCCTGGAAATGATAAAAGTAAATTTCAACAGCGTAGTTATGAGGGGTTAGATGTTTTCTTTGTTc                                                                                             <  1:258650/141‑1 (MQ=255)

                                          aaaaTATTAGTGCTTTGAAACATGGTAAAAACAACTTACGTTTTAAGTTTAGAGGTATTAAGATTCAAGTTTTACTGCCTGGAAATGATAAAAGTAAATTTCAACAGCGTAGTTATGAGGGGTTAGATGTTTTCTTTGTTc                                                                                             <  1:164819/141‑1 (MQ=255)

                                          aaaaTATTAGTGCTTTGAAACATGGTAAAAACAACTTACGTTTTAAGTTTAGAGGTATTAAGATTCAAGTTTTACTGCCTGGAAATGATAAAAGTAAATTTCAACAGCGTAGTTATGAGGGGTTAGATGTTTTCTTTGTTc                                                                                             <  1:77148/141‑1 (MQ=255)

                                          aaaaTATTAGTGCGTTGAAATATGGTAAAAACAACTTACGTTTTAAGTTTAGAGGTAATAAGATTCAAGTTTTACTGCCTGGAAATGATAAAAGTAAATTTCAACAGCGTAGTTATGAGGGGTTAGATGTTTTCTTTGTTc                                                                                             <  1:568298/141‑1 (MQ=255)

                                           aaaTATTAGTGCTTTGAAACATGGTAAAAACAACTTACGTTTTAAGTTTAGAGGTATTAAGATTCAAGTTTTACTGCCTGGGAATGATAAAAGTAAATTTCAACAGCGTAGTTATGAGGGGTTAGATGTTTTCTTTGTTCa                                                                                            >  1:479831/1‑141 (MQ=255)

                                            aaTATTAGTGCTTTGAAACATGGTAAAAACAACTTACGTTTTAAGTTTAGAGGTATTAAGATTCAAGTTTTACTGCCTGGAAATGATAAAAGTAAATTTCAACAGCGTAGTTATGCGGGGTTAGATGATTTCTTTGTTaaa                                                                                           >  2:339957/1‑141 (MQ=255)

                                            aaTATTAGTGCTTTGAAACATGGTAAAAACAACTTACGTTTTAAGTTTAGAGGTATTAAGATTCAAGTTTTACTGCCTGGAAATGATAAAAGTAAATTTCAACAGCGTAGTTATGAGGGGTTAGATGTTTTCTTTGTTCaa                                                                                           >  2:81789/1‑141 (MQ=255)

                                            aaTATTAGTGCTTTGAAACATGGTAAAAACAACTTACGTTTTAAGTTTAGAGGTATTAAGATTCAAGTTTTACTGCCTGGAAATGATAAAAGTAAATTTCAACAGCGTAGTTATGAGGGGTTAGATGTTTTCTTTGTTCaa                                                                                           <  2:194407/141‑1 (MQ=255)

                                            aaTATTAGTGCTTTGAAACATGGTAAAAACAACTTACGTTTTAAGTTTAGAGGTATTAAGATTCAAGTTTTACTGCCTGGAAATGATAAAAGTAAATTTCAAAAGCGTAGTTATAAGGGGTTAGATGTTTTCTTGGTTCaa                                                                                           >  2:47008/1‑141 (MQ=255)

                                            aaTATAAGTGCTTTGAAAAATGGTCAAAACAAATTACGTTTTAAGTTTAGCGCTCGTAAGATTGAAGGTTTACTGTCTGGAACTGTTAAAAGTAAAGTTCAACAGCGTAGTTATGAGGGGTTAGATGTTTTGTTTTTTCaa                                                                                           >  2:216980/1‑141 (MQ=255)

                                               atTAGTGCTTTGAAACATGGTAAAAACAACTTACGTTTTAAGTTTAGAGGTATTAAGATTCAAGTTTTACTGCCTGGAAATGATAAAAGTAAATTTCAACAGCGTAGTTATGAGGGGTTAGATGTTTTCTTTGTTCAAGaa                                                                                        >  2:694278/1‑141 (MQ=255)

                                                          gaacgaTGGTAAAAACAACTTACGTTTTAAGTTTAGAGGTATTAAGATTCAAGTTTCAGCGCGCTTTAATGATAAAAGTAAATTTCAACAGCGTAGTTATGAGGGGTTAGATGTTTTCTTTGTTCAAGAAAAAAGAGATaa                                                                             <  1:186988/136‑1 (MQ=255)

                                                               aTGGTAAAAACAACTTACGTTTTAAGTTTAGAGGTATTAAGATTCAAGTTTTACTGCCTGGAAATGATAAAAGTAAATTTCAACAGCGTAGTTATGAGGGGTTAGATGTTTTCTTTGTTCAAGAAAAAAGAGATAAGCACg                                                                        >  2:777421/1‑141 (MQ=255)

                                                               aTGGTAAAAACAACTTACGTTTTAAGTTTAGAGGTATTAAGATTCAAGTTTTACTGCCTGGAAATGATAAAAGTAAATTTCAACAGCGTAGTTATGAGGGGTTAGATGTTTTCTTTGTTCAAGAAAAAAGAGATAAGCACg                                                                        >  2:637583/1‑141 (MQ=255)

                                                               aTGGTAAAAACAACTTACGTTTTAAGTTTAGAGGTATTAAGATTCAAGTTTTACTGCCTGGAAATGATAAAAGAAAATTTCAACAGCGTAGTTATGAGGGGTTAGATGTTTTCTTTGTTCAAGAAAAAAGAGATAAGCACg                                                                        >  1:735839/1‑141 (MQ=255)

                                                               aTGGTAAAAACAACTTACGTTTTAAGTTTAGAGGTATTAAGATTCAAGTTTTACTGCCTGGAAATGATAAAAGAAAATTTCAACAGCGTAGTTATGAGGGGTTAGATGTTTTCTTTGTTCAAGAAAAAAGAGATAAGCACg                                                                        >  1:1239/1‑141 (MQ=255)

                                                               aTGGTAAAAACAACTTACGTTTTAAGTTTAGAGGTATTAAGATTCAAGTTTTACTGCCTGGAAATGATAAAAGAAAATTTCAACAGCGGAGTTATGAGGGGTTAGATGTTTTCTTTGTTCAAGAAAAAAAAGATATGCACg                                                                        >  1:387603/1‑141 (MQ=255)

                                                                 ggTAAAAACAACTTACGTTTTAAGTTTAGAGGTATTAAGATTCAAGTTTTACTGCCTGGAAATGATAAAAGTAAATTTCAACAGCGTAGTTATGAGGGGTTAGATGTTTTCTTTGTTCAAGAAAAAAGAGATAAGCACGat                                                                      >  1:726282/1‑141 (MQ=255)

                                                                   tAAAAACAACTTACGTTTTAAGTTTAGAGGTATTAAGATTCAAGTTTTACTGCCTGGAAATGATAAAAGTAAATTTCAACAGCGTAGTTATGAGGGGTTAGATGTTTTCTTTGTTCAAGAAAAAAGAGATAAGCACGatat                                                                    <  1:498331/141‑1 (MQ=255)

                                                                   tAAAAACAACTTACGTTTTAAGTTTAGAGGCATTAAGATTCAAGTTTTACTGCCTGGAAATGATAAAAGTAAATTTCAACAGCGTAGTTATGAGGGGTTAGATGTTTTCTTTGTTCAAGAAAAAAGAGACAAGCACGatat                                                                    >  2:776198/1‑141 (MQ=255)

                                                                      aaaCAACTTACGTTTTAAGTTTAGAGGTATTAAGATTCAAGTTTTACTGCCTGGAAATGATAAAAGTAAATTTCAACAGCGTAGTTATGAGGGGTTAGATGTTTTCTTTGTTCAAGAAAAAAGAGATAAGCACGataatat                                                                 >  1:69474/1‑137 (MQ=255)

                                                                      aaaCAACTTACGTTTTAAGTTTAGAGGTATTAAGATTCAAGTTTTACTGCCTGGAAATGATAAAAGTAAATTTCAACAGCGTAGTTATGAGGGGTTAGATGTTTTCTTTGTTCAAGAAAAAAGAGATAAGCACGATATAtt                                                                 >  1:640065/1‑141 (MQ=255)

                                                                      aaaCAACTTACGTTTTAAGTTTAGAGGTATTAAGATTCAAGTTTTACTGCCTGGAAATGATAAAAGTAAATTTCAACAGCGTAGTTATGAGGGGTTAGATGTTTTCTTTGTTCAAGAAAAAAGAGATAAGCACGATATAtt                                                                 >  2:132423/1‑141 (MQ=255)

                                                                           acTTACGTTTTAAGTTTAGAGGTATTAAGATTCAAGTTTTACTGCCTGGAAATGATAAAAGTAAATTTCAACAGCGTAGTTATGAGGGGTTAGATGTTTTCTTTGTTCAAGAAAAAAGAGATAAGCACGATATATTTtata                                                            >  2:684196/1‑141 (MQ=255)

                                                                           acTTACGTTTTAAGTTTAGAGGTATTAAGATTCAAGTTTTACTGCCTGGAAATGATAAAAGTAAATTTCAACAGCGTAGTTATGAGGGGTTAGATGTTTGCTTTGTTCAAGAAAAAAGAGATAAGCACGATATATTTtata                                                            >  2:804058/1‑141 (MQ=255)

                                                                           acTTACGTTTTAAGTTTAGAGGTATTAAGATTCAAGTTTTACTGCCTGGAAATGATAAAAGTAAATTTCAACAGCGTAGTTAGGAGGGGATAGATGTTTTCTTTGTTCAAGGAACAAGAGATAAGCCCGGTATATTTtata                                                            >  2:563516/1‑141 (MQ=255)

                                                                            cTTACGTTTTAAGTTTAGAGGTATTAAGATTCAAGTTTTACTGCCTGGAAATGATAAAAGTAAATTTCAACAGCGTAGTTATGAGGGGTTAGATGTTTTCTTTGTTCAAGAAAAAAGAGATAAGCACGATATATTTTATAc                                                           <  2:333885/141‑1 (MQ=255)

                                                                            cTTACGTTTTAAGTTTAGAGGTATTAAGATTCAAGTTTTACTGCCTGGAAATGATAAAAGTAAATTTCAACAGCGTAGTTATGAGGGGTTAGATGTTTTCTTTGTTCAAGAAAAAAGAGATAAGCACGATATATTTTATAc                                                           <  2:671510/141‑1 (MQ=255)

                                                                             ttACGTTTTAAGTTTAGAGGTATTAAGATTCAAGTTTTACTGCCTGGAAATGATAAAAGTAAATTTCAACAGCGTAGTTATGAGGGGTTAGATGTTTTCTTTGTTCAAGAAAAAAGAGATAAGCACGATATATTTTATACt                                                          <  1:803291/141‑1 (MQ=255)

                                                                              tACGTTTTAAGTTTAGAGGTATTAAGATTCAAGTTTTACTGCCTGGAAATGATAAAAGTAAATTTCAACAGCGTAGTTATGAGGGGTTAGATGTTTTCTTTGTTCAAGAAAAAAGAGATAAGCACGATATATTTTATACTg                                                         >  2:468874/1‑141 (MQ=255)

                                                                              tACCTTTTAATGTTAGAGGGATTAAGATTCAAGTTTTACTGCCTGGAAATGATAAAAGTAAATTTCAACAGCGTAGTTATGAGGGGTTAGATGTTTTCTTTGTTCAAGAAAAAAGAGATAAGCACGATATATTTTATACTg                                                         <  2:688818/141‑1 (MQ=255)

                                                                               aCGTTTTAAGTTTAGAGGTATTAAGATTCAAGTTTTACTGCCTGGAAATGATAAAAGTAAATTTCAACAGCGTAGTTATGAGGGGTTAGATGTTTTCTTTGTTCAAGAAAAAAGAGATAAGCACGATATATTTTATACTGt                                                        <  1:319468/141‑1 (MQ=255)

                                                                                   tttAAGTTTAGAGGTATTAAGATTCAAGTTTTACTGCCTGGAAATGATAAAAGTAAATTTCAACAGCGTAGTTATGAGGGGTTAGATGTTTTCTTTGTTCAAGAAAAAAGAGATAAGCACGATATATTTTATACTGAtgga                                                    >  1:121545/1‑140 (MQ=255)

                                                                                   tttAAGTTTAGAGGTATTAAGATTCAAGTTTTACTGCCTGGAAATGATAAAAGTAAATTTCAACAGCGTAGTTATGAGGGGTTAGATGTTTTCTTTGGCCTAGAAGAGAGGGTTAAGCACGATGTATTTTATACTGTtggt                                                    >  1:149728/1‑141 (MQ=255)

                                                                                   tttAAGATTAGAGGTATTAAGATTCAAGTTTTACTGCCTGGAAATGATAAAAGTAAATTTCAACAGCGTAGTTATGAGGGGTTAGATGTTTTCTTTGTTCAAGAAAAAAGAGATAAGCACGATATATTTTATACTGTtggt                                                    >  2:227106/1‑141 (MQ=255)

                                                                                    ttAAGTTTAGAAGTATTAAGATTCAAGTTTTACTGCCTGGAAATGATAAAAGTAAATGTCAACAGCGTAGTTATGAGGGGTTAGATGTTTTCTTTGTTCAAGAAAAAAGAGATAAGCACGATATATTTTATACTGTtggtg                                                   <  1:238294/141‑1 (MQ=255)

                                                                                      aaGTTTAGGGGTATTAAGATTCAAGTTTTACTGCCTGGAAATGATAAAAGTAAATTTCAACAGCGTAGTTATGAGGGGTTAGATGTTTTCTTTGTTCAAGAAAAAAGAGATAAGCACGATATATTTTATACTGTTggtggt                                                 <  2:682401/141‑1 (MQ=255)

                                                                                        gTTTAGAGGTATTAAGATTCAAGTTTTACTGCCTGGAAATGATAAAAGTAAATTTCAACAGCGTAGTTATGAGGGGTTAGATGTTTTCTTTGTTCAAGAAAAAAGAGATAAGCACGATATATTTTATACTGTTGGTGgtgt                                               >  2:487662/1‑141 (MQ=255)

                                                                                        gTTTAGAGGTATTAAGATTCAAGTTTTACTGCCTGGAAATGATAAAAGTAAATTTCAACAGCGTAGTTATGAGGGGTTAGATGTTTTCTTTGTTCAAGAAAAAAGAGATAAGCACGATATATTTTATACTGTTGGGGgtgt                                               >  2:540210/1‑141 (MQ=255)

                                                                                         tttAGAGGTATTAAGATTCAAGTTTTACTGCCTGGAAATGATAAAAGTAAATTTCAACAGCGTAGTTATGAGGGGTTAGATGTTTTCTTTGTTCAAGAAAAAAGAGATAAGCACGATATATTTTATACTGTTGGTGGTGTa                                              >  2:582900/1‑141 (MQ=255)

                                                                                                gTATTAAGATTCAAGTTTTACTGCCTGGAAATGATAAAAGTAAATTTCAACAGCGTTGTTATGAGGGGTTAGATGTTTTCTTGGTTCAAGAAAAAAGAGATAAGCACGATATATTTTATACTGTTGGTGGTTGAATACAGa                                       >  1:627853/1‑141 (MQ=255)

                                                                                                 tATTAAGAATCAAGTTTTACTGCCTGGAAATGATAAAAGTACATTTCAACAGCGTAGTTATGAGGGGTTAGATGTTGTCTTTGTTCAAGAAAAAAGAGATAAGCACGATATATTTTATACTGTTGGTGGTGTAACACAGaa                                      <  1:168823/141‑1 (MQ=255)

                                                                                                      aGATTCAAGTTTTACTGCCTGGAAATGATAAAAGTAAATTTCAACAGCGTAGTTATGAGGGGTTAGATGTTTTCTTTGTTCAAGAAAAAAGAGATAAGCACGATATATTTTATACTGTTGGTGGTGTAATACAGAataata                                 <  1:778345/141‑1 (MQ=255)

                                                                                                         ttCAAGTTTTACTGCCTGGAAATGATAAAAGTAAATTTCAACAGCGTAGTTATGAGGGGTTAGATGTTTTCTTTGTTCAAGAAAAAAGAGATAAGCACGATATATTTTATACTGTTGGTGGTGTAATACAGAATAATaaaa                              <  1:10606/141‑1 (MQ=255)

                                                                                                         ttCAAGTTTTACTGCCTGGAAATGATAAAAGTAAATTTCAACAGCGTAGTTATGAGGGGTTAGATGTTTTCTTTGTTCAAGAAAAAAGAGATAAGCACGATATATTTTATACTGTTGGTGGTGTAATACAGAATAATaaaa                              <  2:216789/141‑1 (MQ=255)

                                                                                                            aaGTTTTACTGCCTGGAAATGATAAAAGTAAATTTCAACAGCGTAGTTATGAGGGGTTAGATGTTTTCTTTGTTCAAGAAAAAAGAGATAAGCACGATATATCTTATACTGTTGGTGGTGTAATACAGAATAATAAAACAt                           >  2:337391/1‑141 (MQ=255)

                                                                                                            aaGTTTTACTGCCTGGAAATGATAAAAGTAAATTTCAACAGCGTAGTTATGAGGGGTTAGATGTTTTCTTTGTTCAAGAAAAAAGAGATAAGCACGATATATCTTATACTGTTGGTGGTGTAATACAGAATAATAAAACAt                           >  2:763575/1‑141 (MQ=255)

                                                                                                              gTTTTACTGCCTGGAAATGATAAAAGTAAATTTCAACAGCGTAGTTATGAGGGGTTAGATGTTTTCTTTGTTCAAGAAAGAAGAGATAAGCACGATATATTTTATACTTTTGGTGGTGTAATACAGACTATTAAAACAtgt                         >  2:606821/1‑139 (MQ=255)

                                                                                                                              aTGATAAAAGTAAATTTCAACAGCGTAGTTATGAGGGGTTAGATGTTTTCTTTGTTCAAGAAAAAAGAGATAAGCACGATATATTTTATACTGTTGGTGGTGTAATACAGAATAATAAAACATCTGGAGTTGTCAGTGCAc         >  1:210677/1‑141 (MQ=255)

                                                                                                                              aTGATAAAAGTAAATTTCAACAGCGTAGTTATGAGGGGTTAGATGTTTTCTTTGTTCAAGAAAAAAGAGATAAGCACGATATATTTTATACTGTTGGTGGTGTAATACAGAATAATAAAACATCTGGAGTTGTCAGTGCAc         >  2:290141/1‑141 (MQ=255)

                                                                                                                              aTGATAAAAGTAAATTTCAACAGCGTAGTTATGAGGGGTTAGATGTTTTCTTTGTTCAAGAAAAAAGAGATAAGCACGATATATTTTATACTGTTGGTGGTGTAATACAGAATAATAAAACATCTGGAGTTGTCAGTGCAc         >  2:537983/1‑141 (MQ=255)

                                                                                                                               tGAAAAAAGTAAATTTCAACAGCGTAGTTATGAGGGGTTAGATGTTTTCTTTGTTCAAGAAAAAAGAGATAAGCACGATATATTTTATACTGTTGGTGGTGTAATACAGAATAATAAAACATCTGGAGTTGTCAGTGCAcc        <  2:405888/141‑1 (MQ=255)

                                                                                                                               agATAAAAGAAAATTACAACAACGTAATTATGAAGGGTTAGATGTAATAAAAAAAAAAAAAAAAAGAGATAAGCACGATATATTTTATACTGTTGGTGGTGTAATACAGAATAATAAAACATCTGGAGTTGTCAGTGCAcc        <  2:219522/140‑1 (MQ=255)

                                                                                                                                 aTAAAAGTAAATTTCAACAGCGTAGTTATGAGGGGTTAGATGTTTTCTTTGTTCAAGAAAAAAGAGATAAGCACGATATATTTTATACTGTTGGTGGTGTAATACAGAATAATAAAACATCTGGAGTTGTCAGTGCACCaa      <  1:88301/141‑1 (MQ=255)

                                                                                                                                 aTAAAAGTAAATTTCAACAGCGTAGTTATGAGGGGTTAGATGTTTTCTTTGTTCAAGAAAAAAGAGATAAGCACGATATATTTTATACTGTTGGTGGTGTAATACAGAATAATAAAACATCTGGAGTTGTCAGTGCACCaa      <  1:637583/141‑1 (MQ=255)

                                                                                                                                 aTAAAAGTAAATTTCAACAGCGTAGTTATGAGGGGTTAGATGTTTTCTTTGTTCAAGAAAAAAGAGATAAGCACGATATATTTTATACTGTTGGTGGTGTAATACAGAATAATAAAACATCTGGAGTTGTCAGTGCACCaa      <  1:777421/141‑1 (MQ=255)

                                                                                                                                 aTAAAAGAAAATTTCAACAGCGTAGTTATGAGGGGTTAGATGTTTTCTTTGTTCAAGAAAAAAGAGATAAGCACGATATATTTTATACTGTTGGTGGTGTAATACAGAATAATAAAACATCTGGAGTTGTCAGTGCACCaa      <  2:1239/141‑1 (MQ=255)

                                                                                                                                 aTAAAAGAAAATTTCAACAGCGTAGTTATGAGGGGTTAGATGTTTTCTTTGTTCAAGAAAAAAGAGATAAGCACGATATATTTTATACTGTTGGTGGCGTAATACAGAATAATAAAACATCTGGAGTTGTCAGTGCACCaa      <  2:735839/141‑1 (MQ=255)

                                                                                                                                 aTAAAAGAAAATCTCAACAGAGTAATTAGGAGGGGTTAGAGTTTTTAAAAAAAAAAAAAAAAAGAGATAAGCACGATATATTTTATACTGTTGGTGGTGTAATACAGAATAATAAAACATCTGGAGTTGTCAGTGCACCaa      <  2:387603/141‑1 (MQ=255)

                                                                                                                                    aaaGTAAATTTCAACAGTGTAGTTATGTGGGGTTAGATGTTTTGTTTGTTACAGAAAAAAGAGATAAGCACGATATATTTTATACTGTTGGGGTTGTAATACGGAATAATAAAACATCTGGAGTTGTCAGTGCACCAatat   <  2:58717/141‑1 (MQ=255)

                                                                                                                                    aaaGTAAATTTCAACAGCGTAGTTATGAGGGGTTAGATGTTTTCTTTGTTCAAGAAAAAAGAGATAAGCACGATATATTTTATACTGTTGGTGGTGTAATACAGAATAATAAAACATCTGGAGTTGTCAGTGCACCAatat   <  1:709525/141‑1 (MQ=255)

                                                                                                                                    aaaGTAAATTTCAACAGCGGAGTTATGACGGAATCGAAATCTTCTTTGTTCAAGAAAAAAGAGATAAGCACGATATATTTTATACTGTTGGTGGTGTAATACGGAATAATAAAACATCTGGAGTTGTCAGTGCACCAatat   <  2:442493/141‑1 (MQ=255)

                                                                                                                                     aaGTAAATTTCAACAGCGTAGTTATGAGGGGTTAGATGTTTTCTTTGTTCAAGAAAAAAGAGATAAGCACGATATATTTTATACTGTTGGTGGTGTAATACAGAATAATAAAACATCTGGAGTTTTCAGTGCACAattatt  >  1:560359/1‑141 (MQ=255)

AAGCATTATACCGATACTACACTGGAAAGACTATGGAAATGAAAAATATTAGTGCTTTGAAACATGGTAAAAACAACTTACGTTTTAAGTTTAGAGGTATTAAGATTCAAGTTTTACTGCCTGGAAATGATAAAAGTAAATTTCAACAGCGTAGTTATGAGGGGTTAGATGTTTTCTTTGTTCAAGAAAAAAGAGATAAGCACGATATATTTTATACTGTTGGTGGTGTAATACAGAATAATAAAACATCTGGAGTTGTCAGTGCACCAATATT  >  CP000730/456571‑456844

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`