Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 637,932 G→A 5.1% V241V (GTC→GTT thiD ← phosphomethylpyrimidine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730637,9320GA 5.1% 231.0 / 2.4 78V241V (GTC→GTTthiDphosphomethylpyrimidine kinase
Reads supporting (aligned to +/- strand):  ref base G (31/43);  new base A (2/2);  total (33/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.21e-01

GTTGATTTATAGATGAACCGCCTAATCGTTAAACCTCTGTTACTTCAACATCGATATGTTCAATACGGTTGTATGCACCGTGATCCACAGGACCAACAAAATCATTCATTTTCCAACCGTTTTTAATAGCAGAAGCGACGAAAGCTTTCGCGCTAATCACAGCTTCTTTCGGTGACTTACCGTTAGCTAAATATGCAGTTGTTGCCGCAGCAAATGTACAACCAGCACCATGGTTATAACTTTGTTGGAACATGTCTGTTGTTAGTTGA  >  CP000730/637796‑638064
                                                                                                                                        |                                                                                                                                     
gTTGATTTATAGATGAACCGCCTAATCGTTAAACCTCTGTTACTTCAACATCGATATGTTCAATACGGTTGTATGCACCGTGATCCACAGGACCAACAAAATCATTCATTTTCCAACCGTTTTTAATAGCAGAAGcgacga                                                                                                                                   <  2:609747/141‑1 (MQ=255)
 ttGATTTATAGATGAACCGCCTAATCGTTAAACCTCTGTTACTTCAACATCGATATGTTCAATACGGTTGTATGCACCGTGATCCACAGGACCAACAAAATCATTCATTTTCCAACCGTTTTTAATAGCAGAAGCGACGaa                                                                                                                                  <  1:353392/141‑1 (MQ=255)
 ttGATTTATAGATGAACCGCCTAATCGTTAAACCTCTGTTACTTCAACATCGATATGTTCAATACGGTTGTATGCACCGTGATCCACAGGACCAAAAAAATCATTCATTTTCCAACCGTTTTTAATAGCAGAAGCGACGaa                                                                                                                                  <  1:555646/141‑1 (MQ=255)
        ataGATGAACCGCCTAATCGTTAAACCTCTGTTACTTCAACATCGATATGTTCAATACGGTTGTATGCACCGTGATCCACAGGACCAACAAAATCATTCATTTTCCAACCGTTTTTAATACCAGAAGCGACGAAAGCTTTc                                                                                                                           >  1:535918/1‑141 (MQ=255)
                aCCGCCTAATCGTTAAACCTCTGTTACTTCAACATCGATATGTTCAATACGGTTGTATGCACCGTGATCCACAGGACCAACAAAATCATTCATTTTCCAACCGTTTTTAATAGCAGAAGCGACGAAAGCTTTCGCGCTAAt                                                                                                                   >  2:172748/1‑141 (MQ=255)
                aCCGCCTAATCGTTAAACCTCTGTTACTTCAACATCGATATGTTCAATACGGTTGTATGCACCGTGATCCACAGGACCAACAAAATCATTCATTTTCCAACCGTTTTTAATAGCAGAAGCGACGAAAGCTTTCGCGCTAAt                                                                                                                   >  1:196687/1‑141 (MQ=255)
                  cGCCTAATCGTTAAACCTCTGTTACTTCAACATCGATATGTTCAATACGGTTGTATGCACCGTGATCCACAGGACCAACAAAATCATTCATTTTCCAACCGTTTTTAATAGCAGAAGCAACGAAAGCTTTCGCGCTAATca                                                                                                                 >  1:95395/1‑141 (MQ=255)
                  cGCCAAATCGTTAAACCTCTGTTACTTCAACATCGATATGTTCAATACGGTTGTATGCACCGTGATCCACAGGACCAACAAAATCATTCATTTTCCAACCGTTCTTAATAGCAGAAGCAACGAAAGCTTTCGCGCTAATca                                                                                                                 >  1:571703/1‑141 (MQ=255)
                   gtcTAATCGGTAAACCTGTGTTACTTCAACCTCGATATGTTCAATACGGTTGTATGCACCGTGATCCACAGGACCAACAAAATCATTCATTTTCCAACCGTTTTTAATAGCAGAAGCGACGAAAGCTTTCGCGCTAATcac                                                                                                                <  2:230190/139‑1 (MQ=255)
                   gCCTAATCGTTAAACCTCTGTTACTTCAACATCGATATGTTCAATACGGTTGTATGCACCGTGATCCACAGGACCAACAAAATCATTCATTTTCCAACCGTTTTTAATAGCAGAAGCGACGAAAGCTTTCGCGCTAATcac                                                                                                                >  1:757848/1‑141 (MQ=255)
                      tAATCGTTAAACCTCTGTTACTTCAACATCGATATGTTCAATACGGTTGTATGCACCGTGATCCACAGGACCAACAAAATCATTCATTTTCCAACCGTTTTTAATAGCAGAAGCGACGAAAGCTTTCGCGCTAATCACAGc                                                                                                             <  2:279971/141‑1 (MQ=255)
                      tAATCGTTAAACCTCTGTTACTGCAACATCGATATGTTCAATACGGTTGTATGCACCGTGATCCACAGGACCAACAAAATCATTCATTTTCCAACCGTTTTTAATAGCAGAAGCGACGAAAGCTTTCGCGCTAATCACAGc                                                                                                             <  2:102920/141‑1 (MQ=255)
                       aaTCGTTAAACCTCTGTTACTTCAACATCGATATGTTCAATACGGTTGTATGCACCGTGATCCACAGGACCAACAAAATCATTCATTTTCCAACCGTTTTTAATAGCAGAAGCGACGAAAGCTTTCGCGCTAATCACAGct                                                                                                            <  2:122442/141‑1 (MQ=255)
                       aaTCGTTAAACCTCTGTTACTTCAACACCGATATGTTCAATACGGTTGTATGCACCGTGATCCACAGGACCAACAAAATCATTCATTTTCCAACCGTTTTTAATAGCAGTAGCGACGAAAGCTTTCGCGCTAATCACAGct                                                                                                            <  2:7421/141‑1 (MQ=255)
                          cGTTAAACCTCTGTTACGTCCACATCGATATGTTCAATACGGTTGTATGCACCGTGATCCACAGGACCAACAAAATCATTCATTTTCCAACCGTTTTTAATAGCAGAAGCGACGAAAGCTTTCGCGCTAATCACAGcttct                                                                                                         <  1:219373/141‑1 (MQ=255)
                           gTTAAACCTCTGTTACTTCAACATCGATATGTTCAATACGGTTGTATGCACCGTGATCCACAGGACCAACAAAATCATTCATTTTCCAACCGTTTTTAATAGCAGAAGCGACGAAAGCTTTCGCGCTAATCACAGcttctt                                                                                                        >  1:150749/1‑141 (MQ=255)
                                         aCTTCAACATCGATATGTTCAATACGGTTGTATGCACCGTGATCCACAGGACCAACAAAATCATTCATTTTCCAACCGTTTTTAATAGCAGAAGCGACGAAAGCTTTCGCGCTAATCACAGCTTCTTTCGGTGACTTACCg                                                                                          >  1:668130/1‑141 (MQ=255)
                                              aaCATCGATATGTTCAATACGGTTGTATGCACCGTGATCCACAGGACCAACAAAATCATTCATTTTCCAACCGTTTTTAATAGCAGAAGCGACGAAAGCTTTCGCGCTAATCACAGCTTCTTTCGGTGACTTACCGTTAGc                                                                                     >  1:86268/1‑141 (MQ=255)
                                                  tCGATATGTTCAATACGGTTGTATGCACCGTGATCCACAGGACCAACAAAATCATTCATTTTCCAACCGTTTTTAATAGCAGAAGCAACGAAAGCTTTCGCGCTAATCACAGCTTCTTTCGGTGACTTACCGTTAGCTaaa                                                                                 <  2:571703/141‑1 (MQ=255)
                                                  tCGATATGTTCAATACGGTTGTATGCACCGTGATCCACAGGACCAACAAAATCATTCATTTTCCAACCGTTTTTAATAGCAGAAGCAACGAAAGCTTTCGCGCTAATCACAGCTTCTTTCGGTGACTTACCGTTAGCTaaa                                                                                 <  2:95395/141‑1 (MQ=255)
                                                  ccGATATGTTCAATACGGTTGTATGCACCGTGATCCACAGGACCAACAAAATCATTCATTTTCCAACCGTTTTTAATAGCAGAAGCGACGAAAGCTTTCGCGCTAATCACAGCTTCTTTCGGTGACTTACCGTTAGCTaaa                                                                                 <  1:271690/140‑1 (MQ=255)
                                                   cgctatGTTCAATAAGGTTGTATGCACCGTGATCCACAGGACCAACAAAATCATTCATGTTCCAACCGTTTTCAATAGCAGAAGCGACGAAAGCTTTCGCGCTAAGAACAGCTTCTTTCGGTGACTTACCGTTAGCTAAat                                                                                >  2:160135/4‑141 (MQ=255)
                                                   cGATATGTTCAATACGGTTGTATGCACCGTGATCCACAGGACCAACAAAATCATTCATTTTCCAACCGTTTTTAATAGCAGAAGCGACGAAAGCTTTCGCGCTAATCACAGCTTCTTTCGGTGACTTACCGTTAGCTAAat                                                                                >  2:259359/1‑141 (MQ=255)
                                                   cGATATGTTCAATACGGTTGAATGCACCGTGATCCACAGGACCAACAAAATCATACATTTTCCAACCGTTTTTAATAGCAGAAGCGACGAAAGCTTTCGCGCTAATCACAGCTTCTTTCGGTGACTTACCGTTAGCTAAat                                                                                >  1:525479/1‑141 (MQ=255)
                                                        tGTTCAATACGGTTGTATGCACCGTGATCCACAGGACCAACAAAATCATTCATTTTCCAACCGTTTTTAATAGCAGAAGCGACGAAAGCTTTCGCGCTAATCACAGCTTCTTTCGGTGACTTACCGTTAGCTAAATATGCa                                                                           >  1:399765/1‑141 (MQ=255)
                                                         gttccttccattggTATGAACCGTGTTCTACAGGACCAACAAAATCATTCATTTTCCAACCGTTTTTAATAGCAGAAGCGACGAAAGCTTTCGCGCTAATCACAGCTTCTTTCGGTGACTTACCGTTAGCTAAATATGCAg                                                                          <  1:182685/128‑1 (MQ=255)
                                                                 cGGTTGTATGCACCGTGATCCACAGGACCAACAAAATCATTCATTTTCCAACCGTTTTTAATAGCAGAAGCGACGAAAGCTTTCGCGCTAATCACAGCTTCTTTCGGTGACTTACCGTTAGCTAAATATGCAGTTGTTGcc                                                                  >  2:58612/1‑141 (MQ=255)
                                                                 cGGTTGTATGCACCGTGATCCACAGGACCAACAAAATCATTCATTTTCCAACCGTTTTTAATAGCAGAAGCGACGAAAGCTTTCGCGCTAATCACAGCTTCTTTCGGTGACTTACCGTTAGCTAAATATGCAGTTGTTGcc                                                                  >  2:753111/1‑141 (MQ=255)
                                                                       tATGCACCGTGATCCACAGGACCAACAAAATCATTCATTTTCCAACCGTTTTTAATAGCAGAAGCGACGAAAGCTTTCGCGCTAATCACAGCTTCTTTCGGTGACTTACCGTTAGCTAAATATGCAGTTGTTGCCgcagca                                                            <  2:496975/141‑1 (MQ=255)
                                                                       tATGCACCGTGATCCACAGGACCAACAAAATCATTCATTTTCCAACCGTTTTTAATAGCAGAAGCGACGAAAGCTTTCGCGCTAATCACAGCTTCTTTCGGTGACTTACCGTTAGCTAAATATGCAGTTGTTGCCgcagca                                                            <  1:456348/141‑1 (MQ=255)
                                                                       tATGCACCGTGATCCACAGGACCAACAAAATCATTCATTTTCCAACCGTTTTTAATAGCAGAAGCGACGAAAGCTTTCGCGCTAATCACAGCTTCTTTCGGTGACTTACCGTTAGCTAAATATGCAGTTGTTGCCgcagca                                                            <  2:196844/141‑1 (MQ=255)
                                                                       tATGCACCGTGATCCACAGGACCAACAAAATCAATCATTTTCCAACCGTTTTTCATAGCAGAAGCGACGAAAGCTTTCGCGCTAATCACAGCTTCTCTCGGTGACTTACCGTTAGCTAAATATGCAGTTGTTGCCgcagca                                                            <  2:444326/141‑1 (MQ=255)
                                                                       tATCCACCGTGATCTACAGTACCAACAAAATCATTCATTTTCCATCCGTTTTTAATAGCAGAAGCGACTAAAGCTTTCGCGCTAATCACCGCTTCTTTCGGTTACTTACCGTTAGCTAAATATGCAGTTGTTGCCgcagca                                                            <  2:415003/141‑1 (MQ=255)
                                                                         tGCACCGTGATCCACAGGACCAACAAAATCATTCATTTTCCAACCGTTTTTAATAGCAGAAGCGACGAAAGCTTTCGCGCTAATCACAGCTTCTTTCGGGGACTTACCGTTAGCTCAATAGTCAGTTGTTGCCGCAGCaaa                                                          >  1:672641/1‑141 (MQ=255)
                                                                         tGCACCGCGATCCACAGGACCCACACAATCATTCATTTTCCAACCCTTTTTAATAGCAGAAGCGACGAAAGCTTTCGCGCTAATCACAGCTTCTTTCGGTGACTTACCGTTAGCTAAATATGCAGTTGTTGCCGCAGCaaa                                                          <  1:82087/141‑1 (MQ=255)
                                                                         cgCACCGTGATCCACAGGACCAACAAAATCATTCATTTTCCAACCGTTTTTAATAGCAGAAGCGACGAAAGCTTTCGCGCTAATCACAGCTTCTTTCGGTGACTTACCGTTAGCTAAATATGCAGTTGTTGCCGCAGCaaa                                                          <  1:53864/140‑1 (MQ=255)
                                                                             ccGTGATCCACAGGACCAACAAAATCATTCATTTTCCAACCGTTTTTAATAGCAGCCGCGCCGAAACCTTTCGCGCTAATCACAGCTTCTTTCGCTGACTTACCGTTAGCTAAATATGCAGTTGTTACCGCAGCAGATGTa                                                      >  1:73385/1‑141 (MQ=255)
                                                                             ccGTGATCCACAGGACAAACAAAATCATTCATTTTCCAACCGTTTTTAATAGCAGAAGCGACGAAAGCTTTCGCGCTAATCACAGCTTCTTTCGGTGACTTACCATTAGCTAAATATGCAGTTGTTGCCGCAGCAAATTTa                                                      >  1:450253/1‑141 (MQ=255)
                                                                               gTGATCCACAGGACCAACAAAATCATTCATTTTCCAACCGTTTTTAATAGCAGAAGCGACGAAAGCTTTCGCGCTAATCACAGCTTCTTTCGGTGACTTACCGTTAGCTAAATATGCAGTTGTTGCCGCAGCAAATGTACa                                                    <  2:804383/141‑1 (MQ=255)
                                                                                   tCCACAGGACCAACAAAATCATTCGTTACCCAACCGTTTTTAATAGCAGAAGCGACGAAAGCTTTCGCGCTAATCACAGCTTCTTTCGGTGACTTACCGTTAGCTAAATATGCAGTTGTTGCCGCAGCAAATGTACAACCa                                                >  1:799978/1‑141 (MQ=255)
                                                                                     cacaGGACCAACAAAATCATTCATTTTCCAACCGTTTTTAATAGCAGAAGCGACGAAAGCTTTCGCGCTAATCACAGCTTCTTTCGGTGACTTACCGTTAGCTAAATATGCAGTTGTTGCCGCAGCAAATGTACAACCAGc                                              >  1:720075/1‑141 (MQ=255)
                                                                                     cacaGGACCAACAAAATCATTCATTTTCCAACCGTTTTTAATAGCAGAAGCGACGAAAGCTTTCGCGCTAATCACAGCTTCTTTCGGTGACTTACCGTTAGCTAAATATGCAGTTGTTGCCGCAGCAAATGTACAACCAGc                                              <  1:259359/141‑1 (MQ=255)
                                                                                         ggACCAACAAAATCATTCATTTTCCAACCGTTTTTAATAGCAGAAGCGACGAAAGCTTTCGCGCTAATCACAGCTTCTTTCGGTGACTTACCGTTAGCTAAATATGCAGTTGTTGCCGCAGCAAATGTGCAACCAGCACCa                                          >  2:324467/1‑141 (MQ=255)
                                                                                         ggACCAACAAAATCATTCATTTTCCAACCGTTTTTAATAGCAGAAGCGACGAAAGCTTTCGCGCTAATCACAGCTTCTTTCGGTGACTTACCGTTAGCTAAATATGCAGTTGTTGCCGCAGCAAATGTACAACCAGCACCa                                          >  1:689106/1‑141 (MQ=255)
                                                                                         ggACCAACAAAATCATTCATTTTCCAACCGTGTTTAATAGCAGAAGCGACGAAAGCTTTCGCGCTAATCACAGCTTCTTTCGGTGACTTACCGTTAGCTAAATTTGCAGTTGTTGATGAAGCAATTGTActaacggctcct                                          >  1:748953/1‑130 (MQ=255)
                                                                                             caacaaAATCATTCATTTTCCAACCGTTTTTAATAGCAGAAGCGACGAAAGCTTTCGCGCTAATCACAGCTTCTTTCGGTGACTTACCGTTAGCTAAATATGCAGTTGTTGCCGCAGCAAATGTACAACCAGCACCATGGt                                      <  1:113490/141‑1 (MQ=255)
                                                                                                caaAATCATTCATTTTCCAACCGTTTTTAATAGCAGAAGCGACGAAAGCTTTCGCGCTAATCACAGCTTCTTTCGGTGACTTACCGTTAGCTAAATATGCAGTTGTTGCCGCAGCAAATGTACAACCAGCAGCATGGTtat                                   <  1:593045/141‑1 (MQ=255)
                                                                                                caaAATCATTCATTTTCCAACCGTTTTTAATAGCAGAAGCGACGAAAGCTTTCGCGCTAATCACAGCTTCTTTCGGTGACTTACCGTTAGCTAAATATGCAGTTGTTGCCGCAGCAAATGTACAACCAGCACCATGGTtat                                   <  2:500548/141‑1 (MQ=255)
                                                                                                caaAATCATTCATTTTCCAACCGTTTTTAATAGCAGAAGCGACGAAAGCTTTCGCGCTAATCACAGCTTCTTTCGGTGACTTACCGTTAGCTAAATATGCAGTTGTTGCCGCAGCAAATGTACAACCAGCACCATGGTtat                                   <  1:330680/141‑1 (MQ=255)
                                                                                                caaAATCATTCATTTTCCAACCGTTTTTAATAGCAGAAGCGACGAAAGCTTTCGCGCTAATCACAGCTTCTTTCGGTGACTTACCGTTAGCTAAATATGCAGTTGTTGCCGCAGCAAATGTACAACCAGCACCATGGTtat                                   <  1:590963/141‑1 (MQ=255)
                                                                                                caaAATCATTCATTTTCCAACCGTTTTTAATAGCAGAAGCGACGAAAGCTTTCGCGCTAATCACAGCTTCTTTCGGTGACTTACCGTTAGCTAAATATGCAGTTGTTGCCGCAGCAAATGTACAACCAGCACCATGGTtat                                   <  1:689945/141‑1 (MQ=255)
                                                                                                  aaaTCATTCATTTTCCACCCTTTTTTAACAGCAGAAGCGACGAACGTTTTCGCGCTAATCTCCGCTTCTTTCTGTGACGTACCGTTAGCTAAATATGCAGTTGTTGCCGCAGCAAATGTACAACCAGCACCATGGTTATaa                                 <  1:167481/141‑1 (MQ=255)
                                                                                                  aaaTCATTCATTTTCCAACCGTTTTTAATAGCAGAAGCGACGAAAGCTTTCGCGCTAATCACAGCTTCTTTCGGTGACTTACCGTTAGCTAAATATGCAGTTGTTGCCGCAGCAAATGTACAACCAGCACCATGGTTATaa                                 <  2:137074/141‑1 (MQ=255)
                                                                                                  aaaTCATTCATTTTCCAACCGTTTTTAATAGCAGAAGCGACGAAAGCTTTCGCGCTAATCACAGCTTCTTTCGGTGACTTACCGTTAGCTAAATATGCAGTTGTTGCCGCAGCAAATGTACAACCAGCACCATGGTTATaa                                 <  1:699209/141‑1 (MQ=255)
                                                                                                  aaaTCATTCATTTTCCAACCGTTTTTAATAGCAGAAGCGACGAAAGCTTTCGCGCTAATCACAGCTTCTTTCGGTGACTTACCGTTAGCTAAATATGCAGTTGTTGCCGCAGCAAATGTACAACCAGCACCATGGTTATaa                                 <  2:513435/141‑1 (MQ=255)
                                                                                                  aaaTCATTCATTTTCCAACCGTTTTTAATAGCAGAAGCGACGAAAGCTTTCGCGCTAATCACAGCTTCTTTCGGTGACTTACCGTTAGCTAAATATGCAGTTGTTGCCGCAGCAAATGTACAACCAGCACCATGGTTATaa                                 <  1:608831/141‑1 (MQ=255)
                                                                                                          ccttttCCAACCGTTTTTAATAGCAGAAGCGACGAAAGCTTTCCCGCTAATCACAGCTTCTTTCGGTGACTTACCGTTAGCTAAATATGCAGTTGTTGCCGCAGCAAATGTACAACCAGCACCATGGTTATAACTttgttg                         <  2:64714/139‑1 (MQ=255)
                                                                                                           attTTCCAACCGTTTTTAATAGCAGAAGCGACGAAAGCTTTCGCGCTAATCACAGCTTCTTTCGGTGACTTACCGTTAGCTAAATATGCAGTTGTTGCCGCAGCAAATGTACAACCAGCACCATGGTTATAACTTTGTTgg                        <  1:273869/141‑1 (MQ=255)
                                                                                                           attTTCCAACCGTTTTTAATAGCAGAAGCGACGAAAGCTTTCGCGCTAATCACAGCTTCTTTCGGTGACTTACCGTTAGCTAAATATGCAGTTGTTGCCGCAGCAAATGTACAACCAGCACCATGGTTATAACTTTGTTgg                        <  1:274158/141‑1 (MQ=255)
                                                                                                            ttttCCAACCGTTTTTAATAGCAGAAGCGACGAAAGCTTTCGCGCTAATCACAGCTTCTTTCGGTGACTTACCGTTAGCTAAATATGCAGTTGTTGCCGCAGCAAATGTACAACCAGCACCATGGTTATAACTTTGTTGGa                       <  2:463895/141‑1 (MQ=255)
                                                                                                                    ccGTTTTTAATAGCAGAAGTGACGAAAGCTTTCGCGCTAATCACAGCTTCTTTCGGTGACTTACCGTTAGCTAAATATGCAGTTGTTGCCGCAGCAAATGTACAACCAGCACCATGGTTATAACTTTGTTGGAACATGTCt               >  1:443971/1‑141 (MQ=255)
                                                                                                                    ccGTTTTTAATAGCAGAAGCGACGAAAGCTTTCGCTCTAATCACAGCTTCTTTCGGGGACTTACCGCTAGCTAAATATGCAGTTGTTGCCGCAGCAAATGTACAACCAGCACCATGGTTATAACTTTGTTAGacactggct               >  2:520942/1‑133 (MQ=255)
                                                                                                                    ccGTTTTTAATAGCAGAAGCGACGAAAGCTTTCGCGCTAATCACAGCTTCTTTCGGTGACTTACCGTTAGCTAAATATGCAGTTTTTGCCGCGGCAAGTGAACACCCAGCACCATGGTTATCACTTTGTTGGCATATGTCt               >  1:349656/1‑141 (MQ=255)
                                                                                                                    ccGTTTTTAATAGCAGAAGCGACGAAAGCTTTCGCGCTAATCACAGCTTCTTTCGGTGACTTACCGTTAGCTAAATATGCAGTTGTTGCCGCGGCAAATGTACAACCAGCACCATGGTTATAACTTTGTTGGAACATGTCt               >  1:152452/1‑141 (MQ=255)
                                                                                                                    ccGTTTTTAATAGCAGAAGCGACGAAAGCTTTCGCGCTAATCACAGCTTCTTTCGGTGACTTACCGTTAGCTAAATATGCAGTTGTTGCCGCAGCAAATGTACAACCAGCACCATGGTTATAACTTTGTTGGAACATGTCt               <  1:172748/141‑1 (MQ=255)
                                                                                                                    ccGTTTTTAATAGCAGAAGCGACGAAAGCTTTCGCGCTAATCACAGCTTCTTTCGGTGACTTACCGTTAGCTAAATATGCAGTTGTTGCCGCAGCAAATGTACAACCAGCACCATGGTTATAACTTTGTTGGAACATGTCt               >  1:804384/1‑141 (MQ=255)
                                                                                                                    ccGTTTTTAATAGCAGAAGCGACGAAAGCTTTCGCGCTAATCACAGCTTCTTTCGGTGACTTACCGTTAGCTAAATATGCAGTTGTTGCCGCAGCAAATGTACAACCAGCACCATGGGTATAACTTTGTTGGACCATGCCt               >  2:500952/1‑141 (MQ=255)
                                                                                                                      gggtttcagaGCAGAAGCGACGAAAGCTTTCGCGCTAATCACAGCTTCTTTCGGTGACTTACCGTTAGCTAAATATGCAGTTGTTGCCGCAGCAAATGTACAACCAGCACCATGGTTATAACTTTGTTGGAACATGTCtgt            <  1:764192/132‑1 (MQ=255)
                                                                                                                       tttttAATAGCAGAAGCGACGAAAGCTTTCGCGCTAATCACAGCTTCTTTCGGTGACTTACCGTTAGCTAAATATGCAGTTGTTGCCGCAGCAAATGTACAACCAGCACCATGGTTATAACTTTGTTGGAACATGTCtgtt           <  1:761448/141‑1 (MQ=255)
                                                                                                                        ttttAATAGCATAAGCGACGAAAGCTTTCGCGCTAATCACAGCTTCTTTCGGTGACTTACCGTTAGCTAAATATGCAGTTGTTGCCGCAGCAAATGTACAACCAGCACCATGGTTATAACTTTGTTGGAACATGTCtgttg          <  1:74828/141‑1 (MQ=255)
                                                                                                                        ttttAATAGCAGAAGCGACGCAAGCTTTCGCGCTAATCACAGCTTCTTTCGGTGACTTACCGTTAGCTAAATATGCAGTTGTTGCCGCAGCAAATGTACAACCAGCACCATGGTTATAACTTTGTTGGAACATGTCtgttg          <  1:104851/141‑1 (MQ=255)
                                                                                                                        ttttAATAGCAGAAGCGACGAAAGCTTTCGCTCTAATCAGAGCACCGTGCTGCGACTCACCGTTAGCTAAATAGGCCGGCGTTGCCGCAGCAAATGTACACCCAGTACCATGGTTATAACTTTGTTGGAACATGTCtgttg          <  2:390819/141‑1 (MQ=255)
                                                                                                                             aTAGCAGAAGCGACGAAAGCTTTCGCGCTAATCACAGCTTCTTTCGGTGACTTACCGTTAGCTAAATATGCAGTTGTTGCCGCAGCAAATGTACAACCAGCACCATGGTTATAACTTTGTTGGAACATGTCTGTTGTTAGt     <  1:611521/141‑1 (MQ=255)
                                                                                                                             aTAGCAGAAGCGACGAAAGCTTTCGCGCTAATCACAGCTTCTTTCGGTGACTTACCGTTAGCTAAATATGCAGTTGTTGCCGCAGCAAATGTACAACCAGCACCATGGTTATAACTTTGTTGGAACATGTCTGTTGTTAGt     <  2:86268/141‑1 (MQ=255)
                                                                                                                                gCAGAAGCGACGAAAGCTTTCGCGCTAATCACAGCTTCTTTCGGTGACTTACCGTTAGCTAAATATGCAGTTGTTGCCGCAGCAAATGTACAACCAGCACCATGGTTATAACTTTGTTGGAACATGTCTGTTGTTAGTTGa  >  1:463347/1‑141 (MQ=255)
                                                                                                                                gCAGAAGCGACGAAAGCTTTCGCGCTAATCACAGCTTCTTTCGGTGACTTACCGTTAGCTAAATATGCAGTTGTTGCCGCAGCAAATGTACAACCAGCACCATGGTTATAACTTTGTTGGAACATGTCTGTTGTTAGTTGa  <  2:462355/141‑1 (MQ=255)
                                                                                                                                gCAGAAGCGACGAAAGCTTTCGCGCTAATCACAGCTTCTTTCGGTGACTTACCGTTAGCTAAATATGCAGTTGTTGCCGCAGCAAATGTACAACCAGCACCATGGTTATAACTTTGTTGGAACATGTCTGTTGTTAGTTGa  >  1:785945/1‑141 (MQ=255)
                                                                                                                                gCAGAAGCGACGAAAGCTTTCGCGCTAATCACAGCTTCTTTCGGTGACTTACCGTTAGCTAAATATGCAGTTGTTGCCGCAGCAAATGTACAACCAGCACCATGGTTATAACTTTGTTGGAACATGTCTGTTGTTAGTTGa  >  1:768417/1‑141 (MQ=255)
                                                                                                                                gCAGAAGCGACGAAAGCTTTCGCGCTAATCACAGCTTCTTTCGGTAACTTACCGTTAGCTAAATATGCAGTTGTTGCCGCAGCAAATGTACAACCAGCACCATGGTTATAACATTATTGAAACAGTCCGTTTTTTAGTTGa  >  1:391512/1‑141 (MQ=255)
                                                                                                                                        |                                                                                                                                     
GTTGATTTATAGATGAACCGCCTAATCGTTAAACCTCTGTTACTTCAACATCGATATGTTCAATACGGTTGTATGCACCGTGATCCACAGGACCAACAAAATCATTCATTTTCCAACCGTTTTTAATAGCAGAAGCGACGAAAGCTTTCGCGCTAATCACAGCTTCTTTCGGTGACTTACCGTTAGCTAAATATGCAGTTGTTGCCGCAGCAAATGTACAACCAGCACCATGGTTATAACTTTGTTGGAACATGTCTGTTGTTAGTTGA  >  CP000730/637796‑638064

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: