Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 665,392 T→G 100% L79* (TTA→TGA)  iolS → possible acetytransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730665,3920TG99.8% 150.3 / ‑7.1 46L79* (TTA→TGA) iolSpossible acetytransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base G (29/16);  minor base C (1/0);  total (30/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GACAACAGTTAATCACTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACTTAGAAGTAGATAATAAAAATGAAGAA  >  CP000730/665255‑665528
                                                                                                                                         |                                                                                                                                        
gACAACAGTTAATCACTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATgatt                                                                                                                                       >  2:426828/1‑140 (MQ=255)
gACAACAGTTAATCACTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATgata                                                                                                                                       >  1:633105/1‑141 (MQ=255)
gACAACAGTTAATCACTTGTTAGAAGCTTAATATAATGAACATCATTTATAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAGTgata                                                                                                                                       >  1:156113/1‑141 (MQ=255)
gACAACAGTTAATCACTGGTTAGAAGCGTCATATAATGAACATCATTTAAAGAAAAGACTTAAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATgata                                                                                                                                       >  2:73297/1‑141 (MQ=255)
 acaacaGTTCATCACTTGTTAGAAGCTTCATCTCTGGTGCAGCTGGTCAAGAAAAGACTTCAAGGACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGatat                                                                                                                                      >  1:592366/1‑141 (MQ=255)
  caacaGTTAATCACTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATAtt                                                                                                                                     >  1:499083/1‑141 (MQ=255)
         tAATCACTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTACCTCTATTTACGGTGAAAAATGATATTTATCAGc                                                                                                                              >  2:352336/1‑141 (MQ=255)
         tAATCACTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATAATTATCAGc                                                                                                                              >  2:491083/1‑141 (MQ=255)
          actcccagtttAGAAGCTGCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCt                                                                                                                             <  2:427202/132‑1 (MQ=255)
            tCACTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCa                                                                                                                           >  2:788680/1‑141 (MQ=255)
            tCACTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTAAAGACCAATTATTAATAGTAGTTGAAGAAGGAAATGGAATCGTTGGCTTTGCTAACTTTATTTACGGTAAAGAATGATATTGATTAGcgcg                                                                                                                           >  2:279850/1‑138 (MQ=255)
              aCTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCAtt                                                                                                                         <  1:765470/141‑1 (MQ=255)
              aCTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCAtt                                                                                                                         <  1:280372/141‑1 (MQ=255)
               cTTGTTAGAAGCTTCGTATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTAAttt                                                                                                                        >  1:599061/1‑140 (MQ=255)
                ttGTTAGAAGCTTCATATCATGAACATCATTTAAAGAAAAGACTTCAAGCCCAATTATTCTTAGCAGTGGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTCTTTACGGTCAAGAATGATATTTATCAGCTCAttct                                                                                                                       >  2:703766/1‑139 (MQ=255)
                   ttAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGtt                                                                                                                    >  1:176718/1‑141 (MQ=255)
                   ttAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACGTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGtt                                                                                                                    >  1:132240/1‑141 (MQ=255)
                        aGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAAcc                                                                                                               <  1:363322/141‑1 (MQ=255)
                                        aatcatTTAAAGAAAAGATGTCAAGAACAATTATGTTTAGTCGTTGATGAAGGGAATGACATCGTTGGCTTTTCTAACTTTATTTACGGTGAAGAAGGTTATTTATCAGCTCATTATGTTAAACCAGAAGCGCAACATACa                                                                                               <  2:109788/140‑1 (MQ=255)
                                        aatcatTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACa                                                                                               <  2:658998/140‑1 (MQ=255)
                                        aatcatTTAAAGAAAAGACTGCAAGAACAATTATTCTTAGTCGTTGAATAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAATAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACa                                                                                               <  2:109666/140‑1 (MQ=255)
                                          tcatTTAAAGAAAAGAATTCAAGAACAATTATTCTTAGGCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAgg                                                                                             >  1:451767/1‑141 (MQ=255)
                                                   gaaaagaCTTCATGAACACTTATTCTTAGTCGTTGAAGAAGGTAATGTCATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTACCTGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTAc                                                                                    <  2:8663/141‑1 (MQ=255)
                                                   gaaaagaCTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTAc                                                                                    <  1:801999/141‑1 (MQ=255)
                                                   gaaaagaCTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTAc                                                                                    <  2:488759/141‑1 (MQ=255)
                                                    aaaagaCTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACa                                                                                   >  2:288949/1‑141 (MQ=255)
                                                      aagaCTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATAGCAACATACAGGTTATGGTAAAGc                                                                                 >  2:41035/1‑141 (MQ=255)
                                                              aaGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAAGCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTaa                                                                         >  2:72096/1‑141 (MQ=255)
                                                              aaGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCCTTGTTaa                                                                         >  1:400457/1‑141 (MQ=255)
                                                              aaGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTaa                                                                         >  2:211921/1‑141 (MQ=255)
                                                                    aaTTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTACCTTTATTTACGGTGACGAAACTTTCTTATCAGCTAATTATGTTAATCCAGAATCGCAACATACAGTTTATGGTACAGCATTGTTAAATGAAg                                                                   >  1:796235/1‑141 (MQ=255)
                                                                     attagtCGTAGTCGGTGAAGAAGTTAATGACATCGTTGGCTTTGCTAACTTTATTTACCGTGAAGAATGAGATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCAGTGTTAAATGAAgg                                                                  <  1:476810/141‑1 (MQ=255)
                                                                         ttCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGGTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATTGTACAGCATTGTTAAATGAAGGATTa                                                              <  2:484986/141‑1 (MQ=255)
                                                                               gTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACTGCATTGTTAAATGAAGGATTATCACGt                                                        >  2:797239/1‑141 (MQ=255)
                                                                               gTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGGTTATCACGt                                                        >  2:350972/1‑141 (MQ=255)
                                                                               gTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGt                                                        >  2:507915/1‑141 (MQ=255)
                                                                               gTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGt                                                        >  1:420890/1‑141 (MQ=255)
                                                                                        aaagGTAATGACATCATTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGAt                                               <  2:475325/140‑1 (MQ=255)
                                                                                         aagGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGACTGATATTTCTCAGCTCCTTATGTTAAAGTAGAATCGGAACATACAGGTTAAGGTACATCTTTGTTAAATGAAGGAATATCACGGTTTGAAGATa                                              >  2:470001/1‑141 (MQ=255)
                                                                                         aagGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATa                                              >  1:144308/1‑141 (MQ=255)
                                                                                              aaTGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAAttt                                         >  2:499277/1‑141 (MQ=255)
                                                                                                 gACATCGTTGGCTTTTCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGaa                                      <  2:560605/141‑1 (MQ=255)
                                                                                                 gACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGaa                                      >  1:711663/1‑141 (MQ=255)
                                                                                                                gCTCACTTTATTTGCGGTGAAGAATGATATTTTTCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACTTAGaa                       >  2:274394/1‑141 (MQ=255)
                                                                                                                        tatttaCGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAGTTAACGCTGACAAGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACTTAGAAGTAGataa               <  1:352336/141‑1 (MQ=255)
                                                                                                                         atttaCGGTGAAGAATGATATTTATCAGCTCAATATGTTAAAACAGAACCGCAACGTACCGGTTATGGTAGAGCATTGTTAAATGAAGGATTAGCACGTTTTGAAGATAAATTTGAAGGTGTTTACTTAGAAGTAGataat              <  2:592366/141‑1 (MQ=255)
                                                                                                                          tttaCGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACTTAGAAGTAGataata             >  1:6970/1‑141 (MQ=255)
                                                                                                                             aCGGCGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCCCCACACACCGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACTTAGAAGTAGATAATaaaa          >  1:551866/1‑141 (MQ=255)
                                                                                                                                   aagaaTGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACTTAGAAGTAGATAATAAAAATgaag    >  1:312609/1‑141 (MQ=255)
                                                                                                                                     gaaTGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACTTAGAAGTAGATAATAAAAATgaagaa  >  1:716203/1‑141 (MQ=255)
                                                                                                                                         |                                                                                                                                        
GACAACAGTTAATCACTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACTTAGAAGTAGATAATAAAAATGAAGAA  >  CP000730/665255‑665528

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑