Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	667,735	A→C	5.4%	intergenic (+767/‑281)	ywfO → / → adh	phosphohydrolase/alcohol dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	667,735	0	A	C	5.4%	209.2 / 4.4	74	intergenic (+767/‑281)	ywfO/adh	phosphohydrolase/alcohol dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (24/46);  new base C (2/2);  total (26/48)
Fisher's exact test for biased strand distribution p-value = 6.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.90e-01

AAATAATGTCTTAGATTGATTGGGAGTTTTTTTAATTTTTTTGAAATTAAATTAATCTGTAGTTAATAAAAAATTTGAATAACTGACACATTTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCATATCGATTATGTCATTTCTTTTAACATATTAACTAGGGAAACGTTAAAAGTTAACGGTTGATATCTAACTA  >  CP000730/667596‑667875                                                                                                                                            |                                                                                                                                             aagtaatGTCTTAGATTGATTGGGAGTTTTTTTAATTTTTTTGAAATTAAATTAATCTGTAGTTAATAAAAAATTTGAATAACTGACACATTTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATAag                                                                                                                                             <  2:352541/138‑1 (MQ=255) aaaTAATGTCTTAGATTGATTGGGAGTTTTTTTAATTTTTTTGAAATTAAATTAATCTGTAGTTAATAAAAAATTTGAATAACTGACACATTTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATAag                                                                                                                                             <  2:237769/141‑1 (MQ=255) aaaTAATGTCTTAGATTGATTGGGAGTTTTTTTAATTTTTTTGAAATTAAATTAATCTGTAGTTAATAAAAAATTTGAATAACTGACACATTTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATAag                                                                                                                                             <  1:440870/141‑1 (MQ=255) aaaTAATGTCTTAGATTGAGTGGAAGTTTTTTTAATTTTTTTTAAATTAAATTAATCTGTAGTTAATAAAAAATTTGAATAACTGACACATTTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATAag                                                                                                                                             <  1:368195/141‑1 (MQ=255)       tGTCTTAGATTGATTGGGAGTTTTTTTAATTTTTTTGAAATTAAATTAATCTGTAGTTAATAAAAAATTTGAATAACTGACACATTTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATAAGAGTGaa                                                                                                                                       <  1:686622/141‑1 (MQ=255)       tGTCTTAGATTGATTGGGAGTTTTTTTAATTTTTTTGAAATTAAATTAATCTGTAGTTAATAAAAAATTTGAATAACTGACACATTTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATAAGAGTGaa                                                                                                                                       <  1:509881/141‑1 (MQ=255)             aGATTGATTGGGAGTTTTTTTAATTTTTTTGAAATTAAATTAATCTGTAGTTAATAAAAAATTTGAATAACTGACACATTTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATAAGAGTGAAGATAag                                                                                                                                 >  1:231663/1‑141 (MQ=255)             aGATTGATTGGGAGTTTTTTTAATTTTTTTGAAATTAAATTAATCTGTAGTTAATAAAAAATTTGAATAACTGACACATTTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACAATATAATAAGAGTGAAAATaaa                                                                                                                                 >  2:117653/1‑140 (MQ=255)                tttattgGGAGTTTTTTCAATTTTTTTGAAATTAAATTAATCTGTAGTTAATAAAAAATTTGAATAACTGACACATTTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATAAGAGTGAAGATAAGAGt                                                                                                                              <  2:140532/138‑1 (MQ=255)                    ttgGGAGTTTTTTTAATTTTTTTGAAATTAAATTAATATGTAGTTAATAAAAAATTTGAATAACTGACACATTTTTTTGATCATAGCTATATAATTTGTGGTTTAATTCACATTATAATAAGAGTAAAGATAAGAGtatta                                                                                                                          >  2:226860/1‑141 (MQ=255)                      gggAGTTTTTTTAATTTTTTTGAAATTAAATTAATCTGTAGTTAATAAAAAATTTGAATAACTGACACATTTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATAAGAGTGAAGATAAGAGTATtata                                                                                                                        >  1:27281/1‑141 (MQ=255)                           tttttttAATTTTTTTGAAATTAAATTAATCTGTAGTTAATAAAAAATTTGAATAACTGACACATTTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTa                                                                                                                   >  2:402868/1‑141 (MQ=255)                              ttttAATTTTTTANAAAATAAATTAATCTCNAATTAATAAAAAATCTGAATAACTGACAAATTTTTTTGATCATCGCTATATACTTTGTGAATTAATTCAAATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCt                                                                                                                <  1:55763/141‑1 (MQ=255)                               tttAATTTTTTTGAAATTAAATTAATCTGTAGTTAATAAAAAATTTGAATAACTGACACATTTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCtt                                                                                                               <  2:674606/141‑1 (MQ=255)                                    tttttttGAAATTAAATTAATCTGTAGTTAATAAAAAATTTGAATAACTGACACATTTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATACGAGTGAAGATAAGAGTATTATAAATTATCTTtaaat                                                                                                          >  1:706721/1‑141 (MQ=255)                                    tttttttGAAATTAAATTAATCTGTAGTTAATAAAAAATTTGAATAACTGACACATTTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATACGAGTGAAGATAAGAGTATTATAAATTATCTTtaaat                                                                                                          >  1:86907/1‑141 (MQ=255)                                                 aaattaaTCTGTAGTTAATAAAAAATTTGAATAACTGACACATTTTTTTTATTTTTGCTATATACTTTGTGAATTAATTCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGaa                                                                                             >  2:29355/1‑141 (MQ=255)                                                 aaattaaTCTGTAGTTAATAAAAAATTTGAATAACTGACACATTTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGaa                                                                                             >  1:770838/1‑141 (MQ=255)                                                  aattaattTGTAGTTAATAAAAAATTTGAATAACTGACACATTTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAg                                                                                            <  2:489143/141‑1 (MQ=255)                                                  aattaaTCTGTATTTAATAAAAAATTTGAATAACTGACACATTTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACATTATATTAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAg                                                                                            <  1:543440/141‑1 (MQ=255)                                                  aattaaTCTGTAGTTAATAAAAAATTTGAATAACTGACACATTTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAg                                                                                            <  1:320556/141‑1 (MQ=255)                                                  aattaaACTGTAATTAATAAAAAATTTGAATAACTGACACATTTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATAAGAGTGCAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAg                                                                                            <  2:433025/141‑1 (MQ=255)                                                    ttaaTCTGTAGTTAATAAAAAATTTGAATAACTGACACATTTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTa                                                                                          <  1:463688/141‑1 (MQ=255)                                                    ttaaTCTGTAGTTAATAAAAAATTTGAATAACTGACACATTTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTa                                                                                          <  1:270159/141‑1 (MQ=255)                                                    ttaaTCTGTAGTTAATAAAAAATTTGAATAACTGACACATTTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTa                                                                                          >  1:785709/1‑141 (MQ=255)                                                     taaTCTGTAGTTAATAAAAAATTTGAATAACTGACACATTTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTaa                                                                                         >  1:470407/1‑141 (MQ=255)                                                        tCTGTAGTTAATAAAAAATTTGAATAACTGACACATTTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTaaaaa                                                                                      >  2:178997/1‑141 (MQ=255)                                                         cTGTAGTTAATAAAAAATTTGAATAACTGACACATTTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAAAAt                                                                                     <  2:756818/141‑1 (MQ=255)                                                                   taaAGAATTTGAATAACTGACACATGTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACATTCTAATAAGAGTGAAGATACGAGTATTATCAATTATCTTTAATTAAATATATGTGACGGAGAAGTTACACGtttg                                                                           >  2:430083/1‑139 (MQ=255)                                                                   taaAAAATTTGAATAACTGACACATTTTCTTGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAg                                                                           >  2:91639/1‑141 (MQ=255)                                                                      aaaaTTTGAATAACTGACACATTTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATACGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCat                                                                        <  2:706721/141‑1 (MQ=255)                                                                      aaaaTTTGAATAACTGACACATTTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATACGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCat                                                                        <  2:86907/141‑1 (MQ=255)                                                                      aaaaTTTGAATAACTGACACATTTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCat                                                                        <  2:669374/141‑1 (MQ=255)                                                                      aaaaGTGGAATAACTGACACATTTTTTCTATCATATCTATATACTTGGGGAATTTATGCACATGATAAGAAGAGTGAAGATAAGATTATTAGAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCat                                                                        <  1:364574/141‑1 (MQ=255)                                                                       aaaTTTGAATAACTGACACATTTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCata                                                                       >  2:208040/1‑141 (MQ=255)                                                                       aaaTTTGAATAACTGACACATTTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCata                                                                       >  1:774066/1‑141 (MQ=255)                                                                                tAACTGACACATTTTTTTGATTATAGCTATATACGTTGGTAATTAATTCAAATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCATATCGATTATg                                                              <  2:631546/141‑1 (MQ=255)                                                                                tAACTGACACATTTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCATATCGATTATg                                                              >  2:231123/1‑141 (MQ=255)                                                                                 aaCTGACATATTTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCATATCGATTATGt                                                             <  2:697320/141‑1 (MQ=255)                                                                                 aaCTGACACATTTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCATATCGATTATGt                                                             <  2:119257/141‑1 (MQ=255)                                                                                 aaCTAACACATTTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAACATTACACGTTAGCATATCGATTATGt                                                             <  2:502642/141‑1 (MQ=255)                                                                                  aCTGACACATTTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACATTATATTCAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATATATATATGTGAAGTAAAAATTACACGTTAGCATATCGATTATGTc                                                            <  2:431423/141‑1 (MQ=255)                                                                                    tGACACATTTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCATATCTATTCTTTCAt                                                          >  2:130912/1‑141 (MQ=255)                                                                                    tGACACATTTTTTTGATCATAGATATATACTTTGTGAATTAATTCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCATATCGATTATGTCAt                                                          <  2:750880/141‑1 (MQ=255)                                                                                    tGACAAATTTTTTTGATCATAGCTATAAACTTTTTGAATTAATTCAAATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCATATCGATTATGTCAt                                                          <  1:171372/141‑1 (MQ=255)                                                                                    tGAAACATTTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCATATCGATTATGTCAt                                                          <  2:200654/141‑1 (MQ=255)                                                                                     gACACATTTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATAAGAGTGAAGATAAGAGTATTATACATTATCTTTTAATAAATCTATGTGCAGTAAACATTACACGTTAGCATATCGATTATGTCAtt                                                         <  2:799299/141‑1 (MQ=255)                                                                                        ataTTTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCATATCGATTATGTCATTTCt                                                      <  2:79708/139‑1 (MQ=255)                                                                                         caTTTTTTTGATCAGAGCTATATACTTTGTGAATTAATTCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGCAGTAAAAATTACACGTTAGCATATCGATTATGTCATTTCtt                                                     <  2:491455/141‑1 (MQ=255)                                                                                         caTTTTTTTGAGCATAGCTATATATTTTGTTAATTAATTCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCATATCGATTATGTCATTTCtt                                                     <  2:36476/141‑1 (MQ=255)                                                                                             tttttGATCATAGCTATATACTTTGTGAATTAATTCACATTATCTTCAAACTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCATATCGATTATGTCATTTCTTTTaa                                                 <  1:383059/141‑1 (MQ=255)                                                                                             tttttGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCATATCGATTATGTCATTTCTTTTaa                                                 >  2:227364/1‑141 (MQ=255)                                                                                             tttttGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCATATCGATTATGTCATTTCTTTTaa                                                 <  2:351213/141‑1 (MQ=255)                                                                                              ttttGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCATATCGATTATGTCATTTCTTTTAAc                                                <  1:259425/141‑1 (MQ=255)                                                                                              ttttGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCATATCGATTATGTCATTTCTTTTAAc                                                <  1:92752/141‑1 (MQ=255)                                                                                                 tGATCATAGCTATATACTTAGTGAATTAATTCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATCTGTGAAGTAAAAATTACACGTTAGCATATCGATTATGTCATTTCTTTTAACaat                                             >  1:454159/1‑139 (MQ=255)                                                                                                        aGCTATATACTTTGTGAATTAATTCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCATATCGATTATGTCATTTCTTTTAACATATTAACTa                                      <  1:82050/141‑1 (MQ=255)                                                                                                           tatataCTTTGTGAATTAATTCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCATATCGATTATGTCATTTCTTTTAACATATTAACTAgga                                   >  1:341422/1‑140 (MQ=255)                                                                                                            atataCTTTGTGAATTAATTCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCATATCGATTATGTCATTTCTTTTAACATATTAACTAGGGa                                  >  1:153963/1‑141 (MQ=255)                                                                                                             tataCTTTGTGTATTAATTCACATTATAATAAGAGTGAAGATAAGAGTATTATAACTTATCTTTAAATAAATATATGTGAAGTAAAAACTACACGTTAGCATATCGATTATGTCATTTCTTTTAACATATTAACTAGGGaa                                 <  1:218584/141‑1 (MQ=255)                                                                                                             tataCTTTGTGAATTAATTCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCATATCGATTATGTCATTTCTTTTAACATATTAACTAGGGaa                                 <  2:241094/141‑1 (MQ=255)                                                                                                               taCTTTGTGAATTAATTCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCATATCGATTATGTCATTTCTTTTAACATATTAACTAGGGAAAc                               >  2:542174/1‑141 (MQ=255)                                                                                                               taCTTTGTGAATTAATTCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATCATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCATATCGATTATGTCATTTCTTTTAACATATTAACTAGGGAAAc                               <  1:402868/141‑1 (MQ=255)                                                                                                                 cTTTGTGAATTAATTCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTGTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCATATCGATTATGTCATTTCTTTTAACATATTAACTAAGGAAACGt                             >  1:253265/1‑141 (MQ=255)                                                                                                                  tttGTGAATTAATTCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCATATCGATTATGTCATTTCTTTTAACATATTAACTAGGGAAACGtt                            <  1:31428/141‑1 (MQ=255)                                                                                                                   ttGTGAATTAATTCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCATATCGATTATGTCATTTCTTTTAACATATTAACTAGGGAAACGTTa                           <  2:143950/141‑1 (MQ=255)                                                                                                                      tgAATTAATTCAGATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCATATCGATTATGTCATTTCTTTTAACATATTAACTAGGGAAACGTTaaaa                        <  2:455504/141‑1 (MQ=255)                                                                                                                      tgAATTAATTCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCATATCGATTATGTCATTTCTTTTAACATATTAACTAGGGAAACGTTaaaa                        <  2:27281/141‑1 (MQ=255)                                                                                                                       gAATTAATTCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCATATCGATTATGTCATTTCTTTTAACATATTAACTAGGGAAACGTTAAAAg                       <  1:347050/141‑1 (MQ=255)                                                                                                                         attaattCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACAGGGTAGCATATCGATTATGTCATTTCTTTTAAAATATTAACTAGGGGAACGTGAAAAGtg                     >  1:145988/1‑140 (MQ=255)                                                                                                                         attaattCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCATATCGATTATGTCATTTCTTTTAACATATTAACTAGGGAAACGTTAAAAGtt                     >  1:404150/1‑141 (MQ=255)                                                                                                                              ttCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCATATCGATTATGTCATTTCTTTTAACATATTAACTAGGGAAACGTTAAAAGTTAACgg                <  1:231123/141‑1 (MQ=255)                                                                                                                              ttCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCATATCGATTATGTCATTTCTTTTAACATATTAACTAGGGAAACGTTAAAAGTTAACgg                <  2:231663/141‑1 (MQ=255)                                                                                                                              ttCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCATATCGATTATGTCATTTCTTTTAACATATTAACTAGGGAAACGTTAAAAGTTAACgg                <  1:117653/141‑1 (MQ=255)                                                                                                                                cacaTTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCATCTCGATTATGTCATTTCTTTTAACATATTAACTAGGGAAACGTTAAAAGTTAACGGtt              <  2:799254/141‑1 (MQ=255)                                                                                                                                cacaTTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCATATCGATTATGTCATTTCTTTTAACATATTAACTAGGGAAACGTTAAAAGTTAACGGtt              <  1:661073/141‑1 (MQ=255)                                                                                                                                cacaTTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCATATCGATTATGTCATTTCTTTTAACATATTAACTAGGGAAACGTTAAAAGTTAACGGtt              <  1:29355/141‑1 (MQ=255)                                                                                                                                     tataATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCATATCGATTATGTCATTTCTTTTAACATATTAACTAGGGAAACGTTAAAAGTTAACGGTTGatat         <  1:705675/141‑1 (MQ=255)                                                                                                                                       taataaGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCATATCGATTATGTCATTTCTTTTAACATATTAACTAGGGAAACGTTAAAAGTTAACGGTTGATATct       <  1:91639/141‑1 (MQ=255)                                                                                                                                       aaataaGAGTAAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCATATCGATTATGTCATTTCTTTTAACATATTAACTAGGGAAACGTTAAAAGTTAACGGTTGATATct       <  1:404062/140‑1 (MQ=255)                                                                                                                                        aataaGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCATATCGATTATGTCATTTCTTTTAACATATTAACTAGGGAAACGTTAAAAGTTAACGGTTGATATcta      >  2:788173/1‑141 (MQ=255)                                                                                                                                         ataaGAGTGAAGATAAGAGTATTGTAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCATATCGATTATGTCATTTCTTTTAACATATTAACTAGGGAAACGTTAAAAGTTAACGGTTGATATctaa     >  2:372738/1‑141 (MQ=255)                                                                                                                                           aaGAGTGAAGAGAAGAGTATTATAAATTATCTTTAAATAAATAGATGTGAAGTAAAAAGTACACGTTAGCATATCGATTATGTCATTTCTTTTAACATATTAACTAGGGAAACGTTAAAAGTTAACGGTTGATATctaact   <  1:769558/141‑1 (MQ=255)                                                                                                                                            agagGGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCATATCGATTATGTCATTTCTTTTAACATATTAACTAGGGAAACGTTAAAAGTTAACGGTTGATATctaacta  <  1:399471/141‑1 (MQ=255)                                                                                                                                            |                                                                                                                                             AAATAATGTCTTAGATTGATTGGGAGTTTTTTTAATTTTTTTGAAATTAAATTAATCTGTAGTTAATAAAAAATTTGAATAACTGACACATTTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCATATCGATTATGTCATTTCTTTTAACATATTAACTAGGGAAACGTTAAAAGTTAACGGTTGATATCTAACTA  >  CP000730/667596‑667875

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑