Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 76,969 G→A 100% intergenic (+1027/‑107) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073076,9690GA98.1% 262.3 / ‑3.0 91intergenic (+1027/‑107)USA300HOU_0072/USA300HOU_0075universal stress protein/lysophospholipase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (45/42);  minor base T (3/0);  total (49/42)
Fisher's exact test for biased strand distribution p-value = 2.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TCTTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAGGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAATGCATTATATAAAATTTATTGAGTC  >  CP000730/76831‑77101
                                                                                                                                          |                                                                                                                                    
tctTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAagg                                                                                                                                    >  2:265885/1‑141 (MQ=255)
 ctTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTATGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAAGATGGGCTTAAAAGGt                                                                                                                                   <  1:597037/141‑1 (MQ=255)
         cTACGAATATTCGCCAGGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTtat                                                                                                                           <  2:188026/141‑1 (MQ=255)
              gATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGCCATGATACATTAGTGGTTAACTCTATTTTGTTCATTTGCAAAAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATTGt                                                                                                                      >  2:288044/1‑141 (MQ=17)
                   tCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGGTAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTat                                                                                                                 >  1:695061/1‑140 (MQ=255)
                   tCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTAc                                                                                                                 <  2:236660/141‑1 (MQ=255)
                   tCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTAc                                                                                                                 <  2:274645/141‑1 (MQ=255)
                     gCCATGCCACGAAATTAGCCTAATACTTATAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTAAATAGTCTTACTg                                                                                                               >  2:372695/1‑141 (MQ=18)
                       cATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCa                                                                                                             >  2:277049/1‑141 (MQ=255)
                       cATGCCACGAAATTAGCATCATGCTAACAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCa                                                                                                             >  2:502533/1‑141 (MQ=255)
                                  aTTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGAttt                                                                                                  >  2:415973/1‑141 (MQ=255)
                                  aTTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGAttt                                                                                                  >  1:515349/1‑141 (MQ=255)
                                    tAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTgg                                                                                                <  1:177619/141‑1 (MQ=255)
                                     aGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTAAAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTgggg                                                                                               >  2:541732/1‑141 (MQ=255)
                                     aGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGATTCGCTATCTTTTTTTACTTTGCAACAGAACCTATAAAAAACGTGGGCGAAAAAGGTTTATTTATATAGTATTACTGCAAACATTAATTTggg                                                                                               >  2:528046/1‑141 (MQ=18)
                                     aGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATAAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATACGCGTTTACGATTTATTTATATCGTATTAATACCAAAATTGATTTggt                                                                                               >  2:83706/1‑140 (MQ=14)
                                       catcatGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAAAATTGATTTGGGTc                                                                                             >  2:672534/1‑141 (MQ=255)
                                       catcatGCCAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTc                                                                                             <  1:252736/141‑1 (MQ=255)
                                        atcatGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAAAATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTAATGTGGGTCa                                                                                            >  2:316210/1‑141 (MQ=255)
                                        aacagacagacAAGATAAGCGAACACTCACATGATAAATTAGAGGTTAGCTATATTTTTTTACTTTGCAAAAAAAAAAAAAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCa                                                                                            <  2:164499/131‑1 (MQ=21)
                                                  cAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAggg                                                                                  >  1:681592/1‑141 (MQ=255)
                                                     gTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTa                                                                               <  1:378473/141‑1 (MQ=255)
                                                     gTTAAGCGAACACTGACATGATAAATTAGTGGGTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTa                                                                               <  1:514882/141‑1 (MQ=255)
                                                      ttAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCCACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTCGGCCAAAAATTAGGGGTAt                                                                              <  1:453292/141‑1 (MQ=255)
                                                      ttAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCTAAAATTAGGGGTAt                                                                              <  1:443286/141‑1 (MQ=255)
                                                      ttAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTAt                                                                              <  1:685297/141‑1 (MQ=255)
                                                      ttAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTAt                                                                              <  1:648597/141‑1 (MQ=255)
                                                      ttAACCGAACACTGACACTATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACAATGATTTGGGTCAAAAATTAGGGGTAt                                                                              <  1:244533/141‑1 (MQ=37)
                                                      tgaggCGAACACTGCCATGATAAATTAGTGGTTAGCTATAGTTTTTTACTTTGCAACAGAACCTGTAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTAt                                                                              <  2:584169/137‑1 (MQ=37)
                                                           cGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTGAAAAAGTAGGGGTATTaaaa                                                                         >  1:671775/1‑141 (MQ=255)
                                                           cGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTaaaa                                                                         >  1:540216/1‑141 (MQ=255)
                                                           cGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTaaaa                                                                         >  1:267255/1‑141 (MQ=255)
                                                            gAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATGaaaaa                                                                        >  2:28699/1‑141 (MQ=255)
                                                            gAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTAATTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTTCAAACATTGATTTGGGTCAAAAATTAGGGGTTTTagaca                                                                        >  2:80535/1‑137 (MQ=37)
                                                              acacTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTTTTaaaaaaa                                                                      >  1:646033/1‑141 (MQ=255)
                                                              acacTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTGGGGTCACAAATTAGGGGTATTaaaaaaa                                                                      >  1:88852/1‑141 (MQ=255)
                                                                     cATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAatgaatga                                                               >  2:682707/1‑141 (MQ=255)
                                                                            aaTTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAACAAACATGGGCTTAATAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAGATTAGGGGTATTAAAAAAATGAATGAATCATaa                                                        >  2:678310/1‑141 (MQ=255)
                                                                              ttATTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACACGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAAtt                                                      <  2:552927/141‑1 (MQ=255)
                                                                              ttAGTTGTTAGCTATATTTTTTTACTTTGCAATAGAACCTATAAATAAAATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAAtt                                                      <  2:86461/141‑1 (MQ=39)
                                                                                aGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAAtttt                                                    >  1:63142/1‑141 (MQ=255)
                                                                                   ggTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTAtt                                                 >  2:249404/1‑140 (MQ=255)
                                                                                     ttAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAACTGAATGAATCATAATTTTATCaa                                               >  2:611170/1‑141 (MQ=255)
                                                                                     ttAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATAAATCATAATTTTATCaa                                               >  2:410849/1‑141 (MQ=255)
                                                                                     ctAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAGACAAAGGCTTAAGAGGTTACTTTGTATTGTATTTGTGCAAACATTGATTTTGGTCCAAGAGTAGGGGAATTAAAAAAATGAATGAATCATAATTTTATCaa                                               >  2:133431/2‑141 (MQ=255)
                                                                                         cTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTg                                           <  1:415973/141‑1 (MQ=255)
                                                                                         cTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTg                                           <  2:515349/141‑1 (MQ=255)
                                                                                           atatTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGAt                                         <  1:471226/141‑1 (MQ=255)
                                                                                           atatTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAAGTTTATCAAGCTGAt                                         <  1:434129/141‑1 (MQ=255)
                                                                                           atatTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGACTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGAt                                         <  2:686649/141‑1 (MQ=255)
                                                                                                tttttAATTTGAAACAGAAAAAATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGTGTCAAAAATTAAAAAAAAAAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGgag                                    <  2:42349/141‑1 (MQ=255)
                                                                                                 ttttACTTTGCAACAGACCCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGgaga                                   <  1:116162/141‑1 (MQ=255)
                                                                                                 ttttACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGgaga                                   <  1:224043/141‑1 (MQ=255)
                                                                                                 ttttACTTTGCAACAGAACCTATAAAAAACATGGGCGTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGgaga                                   <  1:147976/141‑1 (MQ=255)
                                                                                                    tACTTTGCAACAGAACCTATAAAAAACATGGGCTTGAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGTGGTAGTAAAAAAATGAATGAATCATAATTTTATCAAGCTGCTGGGAAAGGt                                >  1:691306/1‑141 (MQ=255)
                                                                                                      agttGCAAAAAAACCTAAAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGTGTCAAAAATTAAGAGTAATAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTa                              <  2:674684/139‑1 (MQ=255)
                                                                                                       tttttttcAGAACCTATAAAAAACATGGGCTTAAATTGTTAATTTATATAGTATTACTGTAAACTTTGATTTGGGTTTTTTTTTTTGTGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTTATTGGAGGGGTGaa                             >  2:384631/8‑141 (MQ=255)
                                                                                                       tttGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGTAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTaa                             >  2:542020/1‑141 (MQ=255)
                                                                                                       tttGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATACTTTTATCAAGCTGATTGGAGAGGTTaa                             >  2:262948/1‑141 (MQ=255)
                                                                                                       tttGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTaa                             >  1:274481/1‑141 (MQ=255)
                                                                                                          tttttaGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCTAAAATTATGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAAt                          >  2:117511/6‑141 (MQ=255)
                                                                                                          tctaCAGAACCTATAAAAAACACGGGCTTAAATGATTAATTTATATAGTATTACTGCAAACATTGATTTGTGTCATAAATTAGTGATGTTAAAAAAATGAATGAGTCATAATTTTATCAAGCTGAATGGCGTGGTTaaagt                          >  2:487039/4‑139 (MQ=255)
                                                                                                          gCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAAt                          >  1:11003/1‑141 (MQ=255)
                                                                                                          gCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAAAGGTTaaact                          >  2:229488/1‑139 (MQ=255)
                                                                                                          gCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATGATAGAGGGTaaagg                          >  2:156567/1‑139 (MQ=255)
                                                                                                           cAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAATg                         >  1:460860/1‑141 (MQ=255)
                                                                                                           cAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTAGGTCAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAATGc                        >  2:61759/1‑141 (MQ=255)
                                                                                                           aaaaaGAACCTAAAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATAAAAAAAAAAAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTaaatac                         <  2:122276/137‑4 (MQ=255)
                                                                                                               aGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAATGCAtt                     >  1:169207/1‑141 (MQ=255)
                                                                                                               aGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAATGCAtt                     >  1:62392/1‑141 (MQ=255)
                                                                                                               aGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATGTGGGGCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTAGCAAGCTGATTGGAGAGGTTAAAATGCAtt                     >  1:11616/1‑141 (MQ=255)
                                                                                                               aGAACCTATAAAAAACAAGGACTTTATTAAAAAATGTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAATGCAtt                     >  1:193812/1‑141 (MQ=255)
                                                                                                                aaaCATATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGGCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAATGCATta                    <  1:616626/140‑1 (MQ=255)
                                                                                                                  aacaatattaaaCATGGGCTTAAAAGGTTAATTTATATTGGATTACTGCAAACAGTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAATGCATtata                  <  2:186275/132‑1 (MQ=255)
                                                                                                                    cTATAAAAAACATGGGCTTAGAAGGTTAGTTTATATAGTATTACTGCAAACATTTATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAATGCATtatata                >  1:524054/1‑141 (MQ=255)
                                                                                                                    cTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTGAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAATGCATtatata                >  2:380963/1‑141 (MQ=255)
                                                                                                                     tataCAAAACATTGGCTTAAAAGCATAATTGGTATAGTTTTCTTGTCATTACTAAGTTTGGTCAAAAATTAGGTGTATTAAAAAAATGAATAAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAATGCATTATATaa               <  1:789447/141‑1 (MQ=255)
                                                                                                                      ataAAAAACATGGGATTAAAAGGTTAAATTATATAGTATTACTGCAAACATAAAAAAAAAAAAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAATGCATTATATaaa              <  2:635197/141‑1 (MQ=255)
                                                                                                                       taAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATTATAATTTGATCAAGCTGATTGGAGAGGTTAAAATGCATTATATaaaa             <  1:156552/141‑1 (MQ=255)
                                                                                                                       taAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAATGCATTATATaaaa             <  1:655290/141‑1 (MQ=255)
                                                                                                                          aaaaCATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAATGCATTATATACAAttt          >  2:344649/1‑141 (MQ=255)
                                                                                                                             aCATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAATGCATTATATAAAATTTAtt       <  1:758585/141‑1 (MQ=255)
                                                                                                                             aCATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGAGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAATGCATTATATAAAATTTAtt       <  2:291108/141‑1 (MQ=255)
                                                                                                                              cATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAATGCATTATATAAAATTTATTg      >  2:103282/1‑141 (MQ=255)
                                                                                                                               aTGGGCTGAAAAGGTTAATTTATATAGTCTTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATAAAGCTGATTGGAGAGGTTAAAATGCATTATATAAAATATATTGa     >  2:101500/1‑141 (MQ=255)
                                                                                                                                tGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAATGCATTATATAAAATTTATTGAg    <  2:467182/141‑1 (MQ=255)
                                                                                                                                tGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAATGCATTATATAAAATTTATTGAg    <  1:400387/141‑1 (MQ=255)
                                                                                                                                tGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAATGCATTATATAAAATTTATTGAg    <  1:425582/141‑1 (MQ=255)
                                                                                                                                tGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAATGCATTATATAAAATTTATTGAg    >  1:164792/1‑141 (MQ=255)
                                                                                                                                ggggCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAATGCATTATATAAAATTTATTGAg    <  1:324596/140‑1 (MQ=255)
                                                                                                                                 gggCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAATGCATTATATAAAATTTATTGAGt   <  1:249404/141‑1 (MQ=255)
                                                                                                                                  ggCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAATGCATTATATAAAATTTATTGAGTc  <  2:695061/141‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                    
TCTTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAGGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAATGCATTATATAAAATTTATTGAGTC  >  CP000730/76831‑77101

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: