Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 835,012 G→T 6.9% M229I (ATG→ATT prfB → peptide chain release factor 2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730835,0120GT 6.9% 261.5 / 5.6 89M229I (ATG→ATTprfBpeptide chain release factor 2
Reads supporting (aligned to +/- strand):  ref base G (46/35);  new base T (3/4);  total (50/39)
Fisher's exact test for biased strand distribution p-value = 6.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.47e-01

GAATTACCCACCACCCCTCAGGTATAGTTGTTAATAACCAAAATGAACGTTCTCAAATTAAAAACCGTGAAGCAGCTATGAAAATGTTAAAGTCTAAATTATATCAATTAAAATTGGAAGAGCAGGCACGTGAAATGGCTGAAATTCGTGGCGAACAAAAAGAAATCGGCTGGGGAAGCCAAATTAGATCATATGTTTTCCATCCATACTCAATGGTGAAAGATCATCGTACGAACGAAGAAACAGGTAAGGTTGATGCAGTGATGGATGGAGACAT  >  CP000730/834876‑835152
                                                                                                                                         |                                                                                                                                            
gatctACCCACCACCCTCCAGGTATAGCTTTTAATAACCAAAATGAACGTTCTCAAATTAAAAACCGTGAAGCAGCTATGAAAATGCTAAAGTCTAAATTATATCAATTAAGATTGGAAGAGCAGGAACGTGAAATGGCTg                                                                                                                                          <  2:199902/137‑1 (MQ=255)
gAATTACCCACCACCCCTCAGGTATAGTTGTTAATAACCAAAATGAACGTTCTCAAATTAAAAACCGTGAAGCAGCTATGAAAATGTTAAAGTCTAAATTATATCAATTAAAATTGGAAGAGCAGGCACGTGAAATGGCTg                                                                                                                                          <  1:780309/141‑1 (MQ=255)
  aTTACCCACCACCCCTCAGGTATAGTTGTTAATAACCAAAATGAACGTTCTCCACTTAAAAACCGTGAAGCAGCTATGAAAATGTTAAAGTCTAAATTATATCAATTAAAATTGGAAGAGCAGGCACGGGAAATGGCTGaa                                                                                                                                        >  1:24238/1‑141 (MQ=255)
  aTTACCCACCACCCCTCAGGTATAGTTGTTAATAACCAAAATGAACGTTCTCAAATTAAAAACCGTGAAGCAGCTATGAAAATGTTAAAGTCTAAATTATATCAATTAAAATTGGAGGAGCAGGCACGGGAAATGGCTGaa                                                                                                                                        >  1:202537/1‑141 (MQ=255)
  aTTACCCACCACCCCTCAGGTATAGTTGTTAATAACCAAAATGAACGTTCTCAAATTAAAAACCGTGAAGCAGCTATGAAAATGTTAAAGTCTAAATTATATCAATTAAAATTGGAAGAGCAGGCACGTGAAATGGCTGaa                                                                                                                                        >  1:408428/1‑141 (MQ=255)
  aTTACCCACCACCCCTCAGGTATAGTTGTTAATAACCAAAATGAACGTTCTCAAATTAAAAACCGTGAAGCAGCTATGAAAATGTTAAAGTCTAAATTATATCAATTAAAATTGGAAGAGCAGGCACGTGAAATAGCTGaa                                                                                                                                        >  2:770719/1‑141 (MQ=255)
       ccaccaCCCCTCAGGTATAGTTGTTAATAACCAAAATGAACGTTCTCAAATTAAAAACCGTGAAGCAGCTATGAAAATGTTAAAGTCTAAATTATATCAATTAAAATTGGAAGAGCAGGCACGTGAAATGGCTGAAATTCg                                                                                                                                   >  2:302431/1‑141 (MQ=255)
         agcaccacACAGGTATAGATGTTAATAACCAAAATGAACGTTATCAAATTAAAAACCGACAAGCAGCTATGAAAATGTTAAAGTCTAAATTATATCAATGAAAATTGGAAAAGCAGGCACGTGAAATGGCAGaacagcgta                                                                                                                                 >  2:95006/3‑134 (MQ=255)
         accaccCCTCAGGTATAGTTGTTAATAACCAAAATGAACGTTCTCAAATTAAAAACCGTGAAGCAGCTATGAAAATGTTAAAGTCTAAATTATATCAATTAAAATTGGAAGAGCAGGCACGTGAAATGGCTGAAATTCGTg                                                                                                                                 >  2:18074/1‑141 (MQ=255)
         accaccCCTCAGGTATAGTTGTTAATAACCAAAATGAACGTTCTCAAATTAAAAACCGTGAAGCAGCTATGAAAATGTTAAAGTCTAAATTATATCAATTAAAATTGGAAGAGCAGGCACGTGAAATGGCTGAAATTCGTg                                                                                                                                 >  2:183998/1‑141 (MQ=255)
            accCCTCAGGTATAGTTGTTAATAACCAAAATGAACGTTCTCAAATTAAAAACCGTGAAGCAGCTATGAAAATGTTAAAGTCTAAATTATATCAATTAAAATTGGAAGAGCAGGCACGTGAAATGGCTGAAATTCGTGGCg                                                                                                                              <  1:267929/141‑1 (MQ=255)
            accCCGCAGGTATAGTTGTTAATAACCAAAATGAACGTTCTCAAATTAAAAACCGTGAAGCAGCTATGAAAATGTTAAAGTCTAAATTATATCAATTAAAATTGGAAGAGCAGGCACGTGAAATGGCTGAAATTCGTGGCg                                                                                                                              <  2:757803/141‑1 (MQ=255)
              cccTCAGGTATAGTTGTTAATAACCAAAATGAACGTTCTCAAATTAAAAACCGTGAAGCAGCTATGAAAATGTTAAAGTCTAAATTATATCAATTAAAATTGGAAGAGCAGGCACGTGAAATGGCTGAAATTCGTGGCGaa                                                                                                                            >  1:572717/1‑141 (MQ=255)
                 tCAGGTATAGTTGTTAATAACCAAAATGAACGTTCTCAAATTAAAAACCGTGAAGCAGCTATGAAAATGTTAAAGTCTAAATTATATCAATTAAAATTGGAAGAGCAGGCACGTGAAATGGCTGAAATTCGTGGCGAACaa                                                                                                                         <  2:642500/141‑1 (MQ=255)
                 tCAGGTATAGTTGTTAATAACCAAAATGAACGTTCTCAAATTAAAAACCGTGAAGCAGCTATGAAAATGTTAAAGTCTAAATTATATCAATTAAAATTGGAAGAGCAGGCACGTGAAATGGCTGAAATTCGTGGCGAACaa                                                                                                                         <  2:426539/141‑1 (MQ=255)
                 tCAGGTATAGTTGTTAATAACCAAAATGAACGTTCTCAAATTAAAAACCGTGAAGCAGCTATGAAAATGTTAAAGTCTAAATTATATCAATTAAAATTGGAAGAGCAGGCACGTGAAATGGCTGAAATTCGTGGCGAACaa                                                                                                                         <  1:647330/141‑1 (MQ=255)
                 tCAAGTATAGTTGTAAATAACCAAAATGAACAAACAAAAAAAAAAAACCGTGAAGCAGCTATGAAAATGTTAAAGTCTAAATTATATCAATTAAAATTGGAAGAGCAGGCACGTGAAATGGCTGAAATTCGTGGCGAACaa                                                                                                                         <  2:719132/141‑1 (MQ=255)
                   aGGTATAGTTGTTAATAACCAAAATGAACGTTCTCAAATTAAAAACCGTGAAGCGGCTATGAAAATGTTAAAGTCTAAATTATATCAATTAAAATTGGAAGAGCAGGCTCGTGAAATGGCTGAAATTCGTGGCGAACaaaa                                                                                                                       >  1:539946/1‑141 (MQ=255)
                   aGGTATAGTTGTTAATAACCAAAATGAACGTTCTCAAATTAAAAACCGTGAAGCAGCTATGAAAATGTTAAAGTCTAAATTATATCAATTAAAATTGGAAGAGCAGGCACGTGAAATGGCTGAAATTCGTGGCGAACaaaa                                                                                                                       >  1:42299/1‑141 (MQ=255)
                   aGGTATAGTTGTTAATAACCAAAATGAACGTTCTCAAATTAAAAACCGTGAAGCAGCTATGAAAATGTTAAAGTCTAAATTATATCAATTAAAATTGGAAGAGCAGGCACGTGAAATGGCTGAAATTCGTGGCGAACaaaa                                                                                                                       >  2:252877/1‑141 (MQ=255)
                     gTATAGTTGTTAATAACCAAAATGAACGTTCTCAAATTAAAAACCGTGAAGCAGCTATGAAAATGTTAAAGTCTAAATTATATCAATTAAAATTGGAAGAGCAGGCACGTGAAATGGCTGAAATTCGTGGCGAACAAAAAg                                                                                                                     <  2:751860/141‑1 (MQ=255)
                     gTATAGTTGTTAATAACCAAAATGAACGTTCTCAAATTAAAAACCGTGAAGCAGCTATGAAAATGTTAAAGTCTAAATTATATCAATTAAAATTGGAAGAGCAGGCACGTGAAATGGCTGAAATTCGTGGCGAACAAAAAg                                                                                                                     <  1:86952/141‑1 (MQ=255)
                        taGTTGTTAATAACCAAAATGAACGTTCTCAAATTAAACACCGTGCCGAGCGCAAGAATAAGCTAAAGGCAATAGCATATCAATTACAAATAAGAGCCAATTCACGTGGGGTGGCTGAAATTCGGGGCGAACAAAAGGaaa                                                                                                                  >  1:796558/1‑141 (MQ=255)
                        taGTTGTTAATAACCAAAATGAACGTTCTCAAATTAAAAACCGTGAAGCAGCTATGAAAATGTTAAAGTCTAAATTATATCAATTAAAATTGGAAGAGCAGGCACGTGAAATGGCTGAAATTCGTGGCGAACAAAAAGaaa                                                                                                                  >  1:681020/1‑141 (MQ=255)
                        taGTTGTTAATAACCAAAATGAACGTTCTCAAATTAAAAACCGTGAAGCAGCTATGAAAATGTTAAAGTCTAAATTATATCAATTAAAATTGGAAGAGCAGGCACGTGAAATGGCTGAAATTCGTGGCGAACAAAAAGaaa                                                                                                                  >  1:141996/1‑141 (MQ=255)
                        taGTTGTTAATAACCAAAATGAACGTTCTCAAATTAAAAACCGTGAAGCAGCTATGAAAATGTTAAAGTCTAAATTATATCAATTAAAATTGGAAGAGCAGGCACGTGAAATGGCTGAAATTCGTGGCGAAAAAAAAGaaa                                                                                                                  >  2:518241/1‑141 (MQ=255)
                              ttAATACCCAAAAGGAATGTCCTCAAATTAAAAACCGTGAAGCAGCTATGCAAATGTTAAAGTCTAAATTATATCAATTAAAATTGGAAGAGCAGGCACGTGAAATGGCTGAAATTCGTTGCGAACTAAAAGAAATCGGCt                                                                                                            <  2:554822/141‑1 (MQ=255)
                              ttAATAACCAAAATGAACGTTCTCAAATTAAAAACCGTGAAGCAGCTATGAAAATGTTAAAGTCTAAATTATATCAATTAAAATTGGAAGAGCAGGCACGTGAAATGGCTGAAATTCGTGGCGAACAAAAAGAAATCGGcg                                                                                                            >  2:718654/1‑140 (MQ=255)
                                   aaCCAAAATGAACGTTCTCAAATTAAAAACCGTGAAGCAGCTAGGAAAATGTTAAAGTCTAAATTATATCAATTAAAATTGGAAGAGCAGGCACGTGAAATGGCTGAAATTCGTGGCGATCAAAAAGACATCGGCTGGGGa                                                                                                       <  1:421363/141‑1 (MQ=255)
                                    aCCAAAATGAACGTTCTCAAATTAAAAACCGTGAAGCAGCTATGAAAATGTTAAAGTCTAAATTATATCAATTAAAATTGGAAGAGCAGGCACGTGAAATTGCTGAAATTCGTGGCGAACAAAAAGAAATCGGCTGGGGaa                                                                                                      >  2:313985/1‑141 (MQ=255)
                                    aCCAAAATGAACGTTCTCAAATTAAAAACCGTGAAGCAGCTATGAAAATGTTAAAGTCTAAATTATATCAATTAAAATTGGAAGAGCAGGCACGTGAAATTGCTGAAATTCGTGGCGAACAAAAAGAAATCGGCTGGGGaa                                                                                                      >  2:314059/1‑141 (MQ=255)
                                    aCCAAAATGAACGTTCTCAAATTAAAAACCGTGAAGCAGCTATGAAAATGTTAAAGTCTAAATTATATCAATTAAAATTGGAAGAGCAGGCACGAGAAATTGCTGAAATTCGTGGCGAACAAAAAGAAATCGGCTGGGGaa                                                                                                      >  2:495823/1‑141 (MQ=255)
                                      cAAAATGAACGTTCTCAAATTAAAAACCGTGAAGCAGCTATGAAAATGTTAAAGTCTAAATTATATCAATTAAAATTGGAAGAGCAGGCACGTGAAATGGCTGAAATTCGTGGCGAACAAAAAGAAATCGGCTGGGGAAGc                                                                                                    >  1:45571/1‑141 (MQ=255)
                                      cAAAATGAACGTTCTCAAATTAAAAACCGTGAAGCAGCTATGAAAATGTTAAAGTCTAAATTATATCAATTAAAATTGGAAGAGCAGGCACGTGAAATGGCTGAAATTCGTGGCGAACAAAAAGAAATCGGCTGGGGAAGc                                                                                                    >  1:233795/1‑141 (MQ=255)
                                        aaaTGCACGTTCTCAAATTAAAAACCGTGAATCAGCTATGAAAAAGTGAAAGACAAAATAATAACAATTAAAATTAGAAGATCAGGCACGTGAAATGGCTGAAATTCGTGGCGAAGAAAAAGAAATCGGCTGGGGAAGCCa                                                                                                  >  2:385683/1‑141 (MQ=255)
                                         aaTGAACGTTCTCAGATTATAAACCATGAAGCAGCTATGAAAATATTAAAGTCTAAATTATATCAATTAAAATTGGAAGAGCAGGCACGTGAAATGGCTGAACTTCGTGGCGAACAAAAAGAAACCGGCGGGGGAAGCCaa                                                                                                 >  1:443634/1‑141 (MQ=255)
                                         aaTGAACGTTCTCAAATTAAAAACCGTGAAGCAGCTATGAAAATGTTAAAGTCTAAATTATATCAATTAAAATTGGAAGAGCAGGCACGTGAAATGGCTGAAATTCGTGGCGAACAAAAAGAAATCGGCTGGGGAAGCCaa                                                                                                 >  1:474184/1‑141 (MQ=255)
                                         aaTGAACGTTCTCAAATTAAAAACCGTGAAGCAGCTATGAAAATGTTAAAGTCTAAATTATATCAATTAAAATTGGAAGAGCAGGCACGTGAAATGGCTGAAATTCGTGGCGAACAAAAAGAAATCGGCTGGGGAAGCCaa                                                                                                 >  2:707835/1‑141 (MQ=255)
                                         aaTGAACGTTCTCAAATTAAAAACCGTGAAGCAGCTATGAAAATGTTAAAGTCTAAATTATATCAATTAAAATTGGAAGAGCAGGCACGTGAAATGGCTGAAATTCGTGGCGAACAAAAAGAAATCGGCTGGGGAAGCCaa                                                                                                 <  1:391102/141‑1 (MQ=255)
                                         aaTGAACGTTCTCAAATTAAAAACCGTGAAGCAGCTATGAAAATGTTAAAGTCTAAATTATATCAATTAAAATTGGAAGAGCAGGCACGTGAAATGGCTGAAATTCGTGGCGAACAAAAAGAAATCGGCTGGGGAAACCaa                                                                                                 >  1:235422/1‑141 (MQ=255)
                                             aaCGTTCTCAAATTAAAAACCGTGAAGCAGCTATGAAAATGTTAGAGTCTAAATTATATCAATTAAAATTGGAAGAGCAGGCACGTGAAATGGCTGAAATTCGTGGCGAACAAAAAGAAATCGGCTGGGGAAGCCAAATTa                                                                                             >  1:443814/1‑141 (MQ=255)
                                                  tctcAAATTAAAAACCGTGAAGCAGCTATGAAAATGTTAAAGTCTAAATTATATCAATTAAAATTGGAAGAGCAGGCACGTGAAATGGCTGAAATTCGTGGCGAACAAAAAGAAATCGGCTGGGGAAGCCAAATTAGATCa                                                                                        <  1:422997/141‑1 (MQ=255)
                                                     cAAATTAAAAACCGTGCAGCAGCTATGAAAATGTTAAAGTCTAAATTATATCAATTAAAATTGGAAGAGCAGGCACGTTAAACGGCTGAAATTCGTGGCGAACAAAAAGAAATCGGCTGGGGAAGCCAAATTAGATCatat                                                                                     <  1:463800/141‑1 (MQ=255)
                                                     cAAATTAAAAACCGTGAAGCAGCTATGAAAATGTTAAAGTCTAAATTATATCAATTAAAATTGGAAGAGCAGGCACGTGAAATGGCTGAAATTCTTGGCGAACAAAAAGAAATCGGCTGGGGAAGCCAAATTAGATCatat                                                                                     <  2:302415/141‑1 (MQ=255)
                                                     cAAATTAAAAACCGTGAAGCAGCTATGAAAATGTTAAAGTCTAAATTATATCAATTAAAATTGGAAGAGCAGGCACGTGAAATGGCTGAAATTCGTGGCGAACAAAAAGAAATCGGCTGGGGAAGCCAAATTAgctttcgg                                                                                     <  2:612394/141‑8 (MQ=255)
                                                     cAAATTAAAAACCGTGAAGCAGCTATGAAAATGTTAAAGTCTAAATTATATCAATTAAAATTGGAAGAGCAGGCACGTGAAATGGCTGAAATTCGTGGCGAACAAAAAGAAATCGGCTGGGGAAGCCAAATTAGATCatat                                                                                     <  1:661896/141‑1 (MQ=255)
                                                     cAAAGTAAAAACCGTGAAGCAGCTATGAAAATGTTAAAGTCTAAATTATATCAATTAAAATTGGAAGAGCAGGCACGTGAAATGGCTGAAATTCGTGGCGAACAAAAAGAAATCGGCTGGGGAAGCCAAATTAGATCatat                                                                                     <  2:275313/141‑1 (MQ=255)
                                                       aaTTAAAAACCGTGAAGCAGCTATGAAAATGTTAAAGTCTAAATTATATCAATTAAAATTGGAAGAGCAGGCACGTGAAATGGCTGAAATTCGTGGCGAACAAAAAGAAATCGGCTGGGGAAGCCAAATTAGATCATATGt                                                                                   <  1:648370/141‑1 (MQ=255)
                                                       aaTTAAAAACCGTGAAGCAGCTATGAAAATGTTAAAGTCTAAATTATATCAATTAAAATTGGAAGAGCAGGCACGTGAAATGGCTGAAATTCGTGGCGAACAAAAAGAAATCGGCTGGGGAAGCCAAATTAGATCATATGt                                                                                   <  1:408744/141‑1 (MQ=255)
                                                          tAAAAACCGTGAAGCAGCTATGAAAATGTTAAAGTCTAAATTATATCAATTAAAATTGGAAGAGCAGGCACGTGAAATGGCTGAAATTCGTGGCGAACAAAACGAAATCGGCTAGGGAAGCAAAATTAGAACATATGtttt                                                                                >  2:155373/1‑141 (MQ=255)
                                                          tAAAAACCGTGAAGCAGCTATGAAAATGTTAAAGTCTAAATTATATCAATTAAAATTGGAAGAGCAGGCACGTGAAATGGCTGAAATTCGTGGCGAACAAAAAGAAATCGGCTGGGGAAGCCAAATTAGATCATATGtttt                                                                                >  1:530080/1‑141 (MQ=255)
                                                             aaaCCGTGAAACAGCTATGAAAATGTTAAAGTCTAAATTATATCAATTAAAATTGGAAGAGCAGGCACGTGAAATGGCTGAAATTCGTGGCGAACAAAAAGAAATCGGCTGGGGAAGCCAAATTAGATCATATGTTTtcca                                                                             <  2:801783/141‑1 (MQ=255)
                                                              aaCCGTGAAGCAGCTATGAAAATGTTAAAGTCTAAATTATATCAATTAAAATTGGAAGAGCAGGCACGTGAAATGGCTGAAATTCGTGGCGAACAAAAAGAAATCGGCTGGGGAAGCCAAGTTGGATCATATGttttgcca                                                                            >  2:201154/1‑137 (MQ=255)
                                                              aaCCGTGAAGCAGCTATGAAAATGTTAAAGTCTAAATTATATCAATTAAAATTGGAAGAGCAGGCACGTGAAATGGCTGAAATTCGTGGCGAACAAAAAGAAATCGGCTGGGGAAGCCAAATTAGATCATATGTTTtccat                                                                            >  2:651348/1‑141 (MQ=255)
                                                                   tGAAGCAGCTATGAAAATGTTAAAGTCTAAATTATATCAATTAAAATTGGAAGAGCAGGCACGTGAAATGGCTGAAATTCGTGGCGAACAAAAAGAAATCGGCTGGGGAAGTCAAATTAGATCATATGTTTTCCATCCATa                                                                       <  1:32450/141‑1 (MQ=255)
                                                                   tGAAGCAGCTATGAAAATGTTAAAGTCTAAATTATATCAATTAAAATTGGAAGAGCAGGCACGTGAAATGGCTGAAATTCGTGGCGAACAAAAAGAAATCGGCTGGGGAAGCCAAATTAGATCATATGTTTTCCATCCATa                                                                       <  1:47448/141‑1 (MQ=255)
                                                                     aaGCAGCTATGAAAATGTTAAAGTCTAAATTATATCAATTAAAATTGGAAGAGCAGGCACGTGAAATGGCTGAAATTCGTGGCGAACAAAAAGAAATCGGCTGGGGAAGCCAAATTAGATCATATGTTTTCCATCCATACt                                                                     >  1:703402/1‑141 (MQ=255)
                                                                     aaGCAGCTATGAAAATGTTAAAGTCTAAATTATATCAATTAAAATTGGAAGAGCAGACACGTGAAATGGCTGAAATTCGTGGCGAACAAAAAGAAATCGGCTGGGGAAGCCAAATTAGATCATATGTTTTACATCCATACt                                                                     >  2:75234/1‑141 (MQ=255)
                                                                       gcagcTATGAAAATGTTAAAGTCTAAATTATATCAATTAAAATTGGAAGAGCAGGCACGTGAAATGGCTGAAATTCGTGGCGAACAAAAAGAAATCGGCTGGGGAAGCCAAATTAGATCATATGTTTTCCATCCATACTCa                                                                   >  2:721213/1‑141 (MQ=255)
                                                                       gcagcTAAGAAAAGGATAAAGTCTAAATTATATCAATTAAAATTGGAAGAGCAGGCACGTGAAATGGCTGAAATTCGTGGCGAACAAAAAGAAATCGGCTGGGGAAGCCAAATTAGATCATATGTTTTCCATCCATACTCa                                                                   <  2:312036/141‑1 (MQ=255)
                                                                             tATGAAAATGTTAAAGTCTAAATTATATCAATTAAAATTGGAAGAGCAGGCACGTGAAATGGCTGAAATTCGTGGCGAACAAAAAGAAATCGGCTGGGGAAGCCAAATTAGATCATATGTTTTCCATCCATACTCAATGGt                                                              <  1:720011/141‑1 (MQ=255)
                                                                             tATGAAAATGTTAAAGGCTAAATTATATCAATTAAAATTGGAAGAGCAGGCACGTGAAATGGCTGAAATTCGTGGCGAACAAAAAGAAATCGGCTGGGGAAGCCAAATTAGATCATATGTTTTCCATCCATACTCAATGGt                                                              <  2:722642/141‑1 (MQ=255)
                                                                                    aTGTTAAAGTCTAAATTATATCAATTAAAATTGGAAGAGCAGGCACGTGAAATGGCTGAAATTCGTGGCGAACAAAAAGAAATCGGCTGGGGAAGCCAAATTAGATCATATGTTTTCCATCCATACTCAATGGTGAAAGat                                                       >  2:664796/1‑141 (MQ=255)
                                                                                           aGTCTAAATTATATCAATTAAAATTGGAAGAGCAGGCACGTGAAATGGCTGAAATTCGTGGCGAACAAAAAGAAATCGGCTGGGGAAGCCAAATTAGATCATATGTTTTCCATCCATACTCAATGGTGAAAGATCATCGTa                                                >  1:210726/1‑141 (MQ=255)
                                                                                               tAAATTATATCAATTAAAATTGGAAGAGCAGGCACGTGAAATGGCTGAAATTCGTGGCGAACAAAAAGAAATCGGCTGGGGAAGCCAAATTAGATCATATGTTTTCCATCCATACTCAATGGTGAAAGATCATCGTacgaa                                            <  1:609146/141‑1 (MQ=255)
                                                                                                 aaTTATATCAATTAAAATTGGAAGAGCAGGCACGTGAAATTGCTGAAATTCGTGGCGAACAAAAAGAAATCGGCTGGGGAAGTCAAATTAGATCATATGTTTTCCATCCATACTCAATGGTGAAAGATCATCGTacgaacg                                          <  1:495823/141‑1 (MQ=255)
                                                                                                 aaTTATATCAATTAAAATTGGAAGAGCAGGCACGTGAAATTGCTGAAATTCGTGGCGAACAAAAAGAAATCGGCTGGGGAAGCCAAATTAGATCATATGTTTTCCATCCATACTCAATGGTGAAAGATCATCGTacgaacg                                          <  1:314059/141‑1 (MQ=255)
                                                                                                 aaTTATATCAATTAAAATTGGAAGAGCAGGCACGTGAAATTGCTGAAATTCGTGGCGAACAAAAAGAAATCGGCTGGGGAAGCCAAATTAGATCATATGTTTTCCATCCATACTCAATGGTGAAAGATCATCGTacgaacg                                          <  1:313985/141‑1 (MQ=255)
                                                                                                     atatCAATTAAAATTGGAAGAGCAGGCACGTGAAATGGCTGAAATTCGTGGCGAACAAAAAGAAATCGGCTGGGGAAGCCAAATTAGATCATATGTTTTCCATCCATACTCAATGGTGAAAGATCATCGTACGAACgaaga                                      >  1:282590/1‑141 (MQ=255)
                                                                                                     atatCAATTAAAATTGGAAGAGCAGGCACGTGAAATGGCTGAAATTCGTGGCGAACAAAAAGAAATCGGCTGGGGAAGCCAAATTAGATCATATGTTTTCCATCCATACTCAATGGTGAAAGATCATCGTACGAACgaaga                                      >  1:727493/1‑141 (MQ=255)
                                                                                                      tatCAATTAAAATTGGAAGAGCAGGCACGTGAAATGGCTGAAATTCGTGGCGAACAAAAAGAAATCGGCTGGGGAAGCCAAATTAGATCATATGTTTTCCATCCATACTCAATGGTGAAAGATCATCGTACGAACgaagaa                                     >  1:613674/1‑141 (MQ=255)
                                                                                                      tatCAATTAAAATTGGAAGAGCAGGCACGTGAAATGGCTGAAATTCGTGGCGAACAAAAAGAAATCGGCTGGGGAAGCCAAATTAGATCATATGTTTTCCATCCATACTCAATGGTGAAAGATCATCGTACGAACgaagaa                                     >  1:613675/1‑141 (MQ=255)
                                                                                                        tCAATTAAAATTGGAAGAGCAGGCACGTGAAATGGCTGAAATTCGTGGCGAACAAAAAGAAATCGGCTGGGGAAGCCAAATTAGATCATATGTTTTCCATCCATACTCAATGGTGAAAGATCATCGTACGAACGAAGAAAc                                   >  1:354350/1‑141 (MQ=255)
                                                                                                        acAATTAAAATTGGAAGAGCAGGCACGTGAAATGGCTGAAATTCGTGGCGAACAAAAAGAAATCGGCTGGGGAAGCCAAATTAGATCATATGTTTTCCATCCATACTCAATGGTGAAAGATCATCGTACGAACGAAGAAAc                                   <  1:377656/140‑1 (MQ=255)
                                                                                                              aaaaTTGGAAGAGCAGGCACGTGAAATGGCTGAAATTCGTGGCGAACAACGCAACAACGGCTGGGGACCCTTAATTATATCATATGTTTTCCATCCGTACTCAAGGGTGAAAGATCATCGTACGAACGAAGAAACAGGTaa                             >  1:796315/1‑141 (MQ=255)
                                                                                                              aaaaTTGGAAGAGCAGGCACGTGAAATGGCTGAAATTCGTGGCGAACAAAAAGAAATCGGCTGGGGAAGCCAAATTAGATCATATGTTTTCCATCCATACTCAGTGGTGAAAGATCATCGTACGAACGAAGAAACGGGTaa                             >  1:108301/1‑141 (MQ=255)
                                                                                                              aaaaTTGGAAGAGCAGGCACGTGAAATGGCTGAAATTCGTGGCGAACAAAAAGAAATCGGCTGGGGAAGCCAAATTAGATCATATGTTTTCCATCCATACTCAATGGTGAAAGATCATCGTACGAACGAAGAAACAGGTaa                             >  1:8153/1‑141 (MQ=255)
                                                                                                                 aTTGGAAGAGCAGGCACGTGAAATGGCTGAAATTCGTGGCGAACAAAAAGAAATCGGCTGGGGAAGCCAAATTAGATCATATGTTTTCCATCCATACTCAATGGTGAAAGATCATCGTACGAACGAAGAAACAGGTAAGGt                          <  1:721213/141‑1 (MQ=255)
                                                                                                                  attgAAGAGCAGGCACGTGAAATTGCTGAAATGCGTGGCGAACAAAAAGAGATCGGCTGGGGAAGCCAAATTAGATCATATCTTTTCCATCCATACTCAATGGTGAAAGATCATCGTACGAACGAAGAAACAGGTAAGGtt                         <  2:529030/138‑1 (MQ=255)
                                                                                                                         agCAGGCACGTGAAATGGCTGAAATTCGTGGCGAACAAAAAGAAATCGGCTGGGGAAGCCAAATTAGATCATATGTTTTCCATCCATACTCAATGGTGAAAGATCATCGTACGAACGAAGAAACAGGTAAGGTTGATGCAg                  >  1:653164/1‑141 (MQ=255)
                                                                                                                         agCAGGCACGTGAAATGGCTGAAATTCGTGGCGAACAAAAAGAAATCGGCTGGGGAAGCCAAATTAGATCATATGTTTTCCATCCATACTCAATGGTGAAAGATCATCGTACGAACGAAGAAACAGGTAAGGTTGATGCAg                  >  1:124366/1‑141 (MQ=255)
                                                                                                                           cAGGCACGTGAAATGGCTTAAATTCGTGGCGAACAAAAAGAAATCGGCCGGGGAAGCCAAATTAGATCATATGTTTTCCATCCATACTCAATGGGGAAAGATCATCGTACGAACGAAGAAACAGGTAAGGTTGATGCAGTg                <  1:216058/141‑1 (MQ=255)
                                                                                                                           cAGGCACGTGAAATGGCTGAAATTCGTGGCGAACAAAAAGAAATCGGCTGGGGAAGCCAAATTAGATCATATGTTTTCCATCCATACTCAATGGTGAAAGATCATCGTACGAACGAAGAAACAGGTAAGGTTGATGCAGTg                >  1:302597/1‑141 (MQ=255)
                                                                                                                              gCACGTGAAATGGCTGAAATTCGTGGCGAACAAAAAGAAATCGGCTGGGGAAGCCAAATTAGATCATATGTTTTCCATCCATACTCAATGGTGAAAGATCATCGTACGAACGAAGAAACAGGTAAGGTTGGTGCAGTgatg             >  1:89588/1‑141 (MQ=255)
                                                                                                                              gCACGTGAAATGGCTGAAATTCGTGGCGAACAAAAAGAAATCGGCTGGGGAAGCCAAATTAGATCATATGTTTTCCATCCATACTCAATGGGGAAAGATCATCGTACGAACGAAGAAACAGGTAAGGTTGATGCAGTgatg             >  1:208826/1‑141 (MQ=255)
                                                                                                                                  gTGAAATGGTTGAAATTCGTGGCGAACAAAAAGAAATCGGCTGGGGAAGCCAAATTAGATCATATGTTTTCCATCCATACTCAATGGTGAAAGATCATCGTACGAACGAAGAAACAGGTAAGGTTGATGCAGTgatggatg         <  1:95006/141‑1 (MQ=255)
                                                                                                                                  gTGAAATGGCTGAAATTCGTGGCGAACAAAAAGAAATCGGCTGGGGCACCAAAATTAGATCATATGTTTTCCATCCATACTCAATGGTGAAAGATCATCGTACGAACGAAGAAACAGGTAAGGTTGATGCAGTgatggatg         <  1:183998/141‑1 (MQ=255)
                                                                                                                                  gTGAAATGGCTGAAATTCGTGGCGAACAAAAAGAAATCGGCTGGGGAAGCCAAATTAGATCATATGTTTTCCATCCATACTCAATGGTGAAAGATCATCGTACGAACGAAGAAACAGGTAAGGTTGATGCAGTgatggatg         <  1:18074/141‑1 (MQ=255)
                                                                                                                                   ggAAATGGCTGAAATTCGTGGCGAACAAAAAGAAATCGGCTGGGGAAGCCAAATTAGATCATATGTTTTCCATCCATACTCAATGGTGAAAGATCATCGTACGAACGAAGAAACAGGTAAGGTTGATGCAGTGatggatgg        <  2:58345/140‑1 (MQ=255)
                                                                                                                                         ggCTGAAATTCGTGGCGAACAAAAAGAAATCGGCTGGGGAAGCCAAATTAGATCATATGTTTTCCATCCATACCCAATGGTGAAAGATCATCGTACGAACGAAGAAACAGGTAAGGTTGATGCAGTGATGGATGGAGACAt  <  2:572717/141‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                            
GAATTACCCACCACCCCTCAGGTATAGTTGTTAATAACCAAAATGAACGTTCTCAAATTAAAAACCGTGAAGCAGCTATGAAAATGTTAAAGTCTAAATTATATCAATTAAAATTGGAAGAGCAGGCACGTGAAATGGCTGAAATTCGTGGCGAACAAAAAGAAATCGGCTGGGGAAGCCAAATTAGATCATATGTTTTCCATCCATACTCAATGGTGAAAGATCATCGTACGAACGAAGAAACAGGTAAGGTTGATGCAGTGATGGATGGAGACAT  >  CP000730/834876‑835152

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: