Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 903,873 A→G 6.3% intergenic (+148/‑523) USA300HOU_0861 → / → USA300HOU_0862 possible DNA primase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730903,8730AG 6.3% 179.3 / 3.0 65intergenic (+148/‑523)USA300HOU_0861/USA300HOU_0862possible DNA primase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (36/23);  new base G (2/2);  total (38/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.40e-01

CGAATGCAACCGAATGCAACCGAAAATTTTAAAACGGGTTGCACCTATAATCACAGTTATATCAAGGGTTTAGGGCACTTATGCAACTGAGCAACTCAAAATCTATAACTTATTAGTAGAAAAGTAAATAACCAAAATATAAATAAGAAATAGTATCAAAAATTATATAAACTAATTTGTGTGATTTCGGTTGCATAGTTGCAAAAGTGGATTAACCCCTTGTGACAGTAAGAAAAAAATTGCAACTGGAAAATTTAAAACGAGTTGCATAGCTGTAAATT  >  CP000730/903733‑904013
                                                                                                                                            |                                                                                                                                            
cgaatgcaaccgaatgcaaccgaaAATTTTAAAACGGGTTGCACCTATAATCACAGTTATATCAAGGGTTTAGGGCACTTATGCAACTGAGCAACTCAAAATCTATAACTTATTAGTAGAAAAGTAAATAACCAAAAtata                                                                                                                                              <  2:211505/141‑1 (MQ=255)
 gaatgcaaccgaatgcaaccgaaAATTTTAAAACGGGTTGCACCTATAATCACAGTTATATCAAGGGTTTAGGGCACTTATGCAACTGAGCAACTCAAAATCTATCACTTATTAGTAGAAAGGTAAATAACCAAAATataa                                                                                                                                             >  1:182153/1‑141 (MQ=255)
 gaatgcaaccgaatgcaaccgaaAATTTTAAAACGGGTTGCACCTATAATCACAGTTATATCAAGGGTTTAGGGCACTTATGCAACTGAGCAACTCAAAATCTATAACTTATTAGTAGAAAAGTAAATAACCAAAATataa                                                                                                                                             >  2:73621/1‑141 (MQ=255)
   atgcaaccgaatgcaaccgaaAATTTTAAAACGGGTTGCACCTATAATCACAGTTATATCAAGGGTTTAGGGCACTTATGCAACTGAGCAACTCAAAATCTATAACTTATTAGTAGAAAAGTAAATAACCAAAATataaaa                                                                                                                                           >  2:321808/1‑140 (MQ=255)
   atgcaaccgaatgcaaccgaaAATTTTAAAACGGGTTGCACCTATAATCACAGTTATATCAAGGGTTTAGGGCACTTATGCAACTGAGCAACTCAAAATCTATAACTTATTAGTAGAAAAGTAAATAACCAAAATataaaa                                                                                                                                           >  2:75227/1‑140 (MQ=255)
   atgcaacagaatgcaaccgagAATTTTAAAACGGGTTGCACCTATAATCACAGTTATATCAAGGGTTTAGGGCACTTATGCAACTGAGCAACTCAAAATCTATAACTTATTAGTAGAGAAGTAAATAACCAAAATataaat                                                                                                                                           >  1:510952/1‑141 (MQ=255)
    tgcaaccgaatgcaaccgaaAATTTTAAAACGGGTTGCACCTATAATCACAGTTATATCAAGGGTTTAGGGCACTTATGCAACTGAGCAACTCAAAATCTATAACTTATTAGTAGAAAAGTAAATAACCAAAATataaata                                                                                                                                          >  2:359798/1‑141 (MQ=255)
    tgcaaccgaatgcaaccgaaAATTTTAAAACGGGTTGCACCTATAATCACAGTTATATCAAGGGTTTAGGGCACTTATGCAACTGAGCAACTCAAAATCTATAACTTATTAGTAGAAAAGTAAATAACCAAAATataaata                                                                                                                                          >  2:421914/1‑141 (MQ=255)
     gcaaccgaatgcaaccgaaAATTTTAAAACGGGTTGCACCTATAATCACAGTTATATCAAGGGTTTAGGGCACTTCTGCAACTGAGCAACTCAAAATCTATAACTTATTAGTAGAAAAGTAAATAACCAAAATataaataa                                                                                                                                         >  2:788379/1‑141 (MQ=255)
     gcaaccgaatgcaaccgaaAATTTTAAAACGGGTTGCACCTATAATCACAGTTATATCAAGGGTTTAGGGCACTTATGCAACTGAGCAACTCAAAATCTATAACTTATTAGTAGAAAAGTAAATAACCAAAATataaataa                                                                                                                                         <  2:752598/141‑1 (MQ=255)
             atgtaaccgaaAATTTTAAAACGGGTTGCACCTATAATCACAGTTATATCAAGGGTTTAGGGCACTTATGCAACTGAGCAACTCAAAATCTATAACTTATTAGTAGAAAAGTAAATAACCAAAATATAAATAAGAAATAGt                                                                                                                                 <  2:335630/141‑1 (MQ=255)
              tgcaaccgaaAATTTTAAAACGGGTTGCACCTATAATCACAGTTATATCAAGGGTTTAGGGCACTTATGCAACTGAGCAACTCAAAATCTATAACTTATTAGTAGAAAAGTAAATAACCAAAATATGAATAAGAAATAGTa                                                                                                                                >  1:790269/1‑141 (MQ=255)
              tgcaaccgaaAATTTTAAAACGGGTTGCACCTATAATCACAGTTATATCAAGGGTTTAGGGCACTTATGCAACTGAGCAACTCAAAATCTATAACTTATTAGTAGAAAAGTAAATAACCAAAATATGAATAAGAAATAGTa                                                                                                                                >  2:136546/1‑141 (MQ=255)
                 aaccgaaAATTTTAAAACGGGTTGCACCTATAATCACAGTTATATCAAGGGTTTAGGGCACTTATGCAACTGAGCAACTCAAAATCTATAACTTATTAGTAGAAAAGTAAATAACCAAAATATAAATAAGAAATAGTATCa                                                                                                                             >  2:614829/1‑141 (MQ=255)
                  accgaaAATTTTAAAACGGGTTGCACCTATAATCACAGTTATATCAAGGGTTTAGGGCACTTATGCAACTGAGCAACTCAAAATCTATAACTTATTAGTAGAAAAGTAAATAACCAAAATATAAATAAGAATTAGTATCaa                                                                                                                            >  1:439227/1‑141 (MQ=255)
                  accgaaAATTTTAAAACGGGTTGCACCTATAATCACAGTTATATCAAGGGTTTAGGGCACTTATGCAACTGAGCAACTCAAAATCTATAACTTATTAGTAGAAAAGTAAATAACCAAAATATAAATAAGAAATAGTATCaa                                                                                                                            >  1:145891/1‑141 (MQ=255)
                  accgaaAATTTTAAAACGGGTTGCACCTATAATCACAGTTATATCAAGGGTTTAGGGCACTTATGCAACTGAGCAACTCAAAATCTATAACTTATTAGTAGAAAAGTAAATAACCAAAATATAAATAAGAAATAGTATCaa                                                                                                                            >  1:407784/1‑141 (MQ=255)
                  accgaaAATTTTAAAACGGGTTGCACCTATAATCACAGTTATATCAAGCGTTGACGGCACTTATGCAACTGAGCAACTCAAAATCTATAACTTATTCGTAGAAAAGTAAATAACAAAAATATAAATGAGAACTAGTGTCaa                                                                                                                            >  1:135341/1‑141 (MQ=255)
                            ttAAAACGGGTTGCACCTATAATCACAGTTATATCAAGGGTTTAGGGCACTTATGCAACTGAGCAACTCAAAATCTATAACTTATTAGTAGAAAAGTGAATAACCAAAATATAAATAAGAAATAGTATCAAAAATtagata                                                                                                                  >  1:616263/1‑141 (MQ=255)
                            ttAAAACGGGTTGCACCTATAATCACAGTTATATCAAGGGTTTAGGGCACTTATGCAACTGAGCAACTCAAAATCTATAACTTATTAGTAGAAAAGTAAATAACCAAAATATAAATAAGAAATAGTATCAAAAATtatata                                                                                                                  <  1:305537/141‑1 (MQ=255)
                               aaaCGGGTTGCACCTATAATCACAGTTATATCAAGGGTTTAGGGCACTTATGCAACTGAGCAACTCAAAATCTATAACTTATTAGTAGAAAAGTAAATAACTACAATATAAATAAGAAATAGTATCAAAAATTATATAAAc                                                                                                               <  2:279917/141‑1 (MQ=255)
                               aaaCGGGTTGCACCTATAATCACAGTTATATCAAGGGTTTAGGGCACTTATGCAACTGAGCAACTCAAAATCTATAACTTATTAGTAGAAAAGTAAATAACCAAAATATAAATAAGAAATAGTATCAAAAATTATATAAAc                                                                                                               <  2:543168/141‑1 (MQ=255)
                               aaaCGGGTTGCACCTATAATCACAGTTATATCAAGGGTTTAGGGCACTTATGCAACTGAGCAACTCAAAATCTATAACTTATTAGTAGAAAAGTAAATAACCAAAATATAAATAAGAAATAGTATCAAAAATTATATAAAc                                                                                                               <  2:621522/141‑1 (MQ=255)
                               aaaCGGGTTGCACCTATAATCACAGTTATATCAAGGGTTTAGGGCACTTATGCAACTGAGCAACTCAAAATCTATAACTTATTAGTAGAAAAGTAAATAACCAAAATATAAATAAGAAATAGTATCAAAAATTATATAAAc                                                                                                               <  2:291929/141‑1 (MQ=255)
                                   gggTTGCACCTATAATCACAGTTATATCAAGGGTTTAGGGCACTTATGCAACTGAGCAACTCAAAATCTATAACTTATTAGTAGACAAGTAAATAACCAAAATATAAATAAGAAATAGTATCAAAAATTATATAAAATagt                                                                                                           >  1:186218/1‑139 (MQ=255)
                                        gCACCTATAATCACAGTTATATCAAGGGTTTAGGGCACTTATGCAACTGAGCAACTCAAAATCTATAACTTATTAGTAGAAAAGTAAATAACCAAAATATAAATAAGAAATAGTATCAAAAATTATATAAACTAATTtgtg                                                                                                      >  1:399258/1‑141 (MQ=255)
                                        gCACCTATAATCACAGTTATATCAAGGGTTTAGGGCACTTATGCAACTGAGCAACTCAAAATCTATAACTTATTAGTAGAAAAGTAAATAACCAAAATATAAATAAGAAATAGTATCAAAAATTATATAAACTAATTtgtg                                                                                                      >  1:641565/1‑141 (MQ=255)
                                        gCACCTATAATCACAGTTATATCAAGGGTTTAGGGCACTTATGCAACTGAGCAACTCAAAATCTATAACTTATTAGTAGAAAAGTAAATAACCAAAATATAAATAAGAAATAGTATCAAAAATTATATAAACTAATTtgtg                                                                                                      >  1:291331/1‑141 (MQ=255)
                                          aacTATAATCACAGTTATATCAAGGGTTTAGGGCACGTATGCAACTGAGCAACTCAAAATCTATAACTTATTAGTAGAAAAGTAAATAACCAAAATATAAATAAGAAATAGTATCAAAAATTATATAAACTAATTtgtgtg                                                                                                    <  1:233608/139‑1 (MQ=255)
                                            cTATAATCACAGTTATATCAAGGGTTTAGGGCACTTATGCAACTGAGCAACTCAAAATCTATAACTTATTAGTAGAAAAGTAAATAACCAAAATATAAATAAGAAATAGTATCAAAAATTATATAAACTAATTTGTGTGAt                                                                                                  >  1:313524/1‑141 (MQ=255)
                                            cTATAATCACAGTTATATCAAGGGTTTAGGGCACTTATGCAACTGAGCAACTCAAAATCTATAACTTATTAGTAGAAAAGTAAATAACCAAAATATAAATAAGAAATAGTATCAAAAATTATATAAACTAAATTGTGTGAt                                                                                                  >  1:241881/1‑141 (MQ=255)
                                                 aTCACAGTTATATCAAGGGTTTAGGGCACTTATGCAACTGAGCAACTCAAAATCTATAACTTATTAGTAGAAAAGTAAATAACCAAAATATAAATAAGAAATAGTATCAAAAATTATATAAACTAATTTGTGTGATTTCgg                                                                                             <  1:614829/141‑1 (MQ=255)
                                                  tCACAGTAATATAAAGGGTTTAGGGCACTTATGCAACTGAGCAACTCAAAATCTATAACTTATTAGTAGAAAAGTAAATAACCAAAATATAAATAAGAAATAGTATCAAAAATTATATAAACTAATTTGTGTGATTTCGGt                                                                                            <  2:135341/141‑1 (MQ=255)
                                                   cagagtttaattatgggTTTAGGGCACTTATGCAACTGAGCAACTCAAAATCTATAACTTATTAGTAGAAAAGTAAATAACCAAAATATAAATAAGAAATAGTATCAAAAATTATATAAACTAATTTGTGTGATTTCGGtt                                                                                           <  2:423358/127‑1 (MQ=255)
                                                   cacaGTTATATCAAGGGTTTAGGGCACTTATGCAACTGAGCAACTCAAAATCTATAACTTATTAGTAGAAAAGTAAATAACCAAAATATAAATAAGAAATAGTATCAAAAATTATATAACCTAATTTGTGTAATTTCGGtt                                                                                           >  2:313398/1‑141 (MQ=255)
                                                   cacaGTTATATCAAGGGTTTAGGGCACTTATGCAAATGAGCAACTCAAAATCTATAACTTATTAGTAGAAAAGTAAATAACAAAAATATAAATAAGAAATAGTATAAAAAAATATATAAACTAATTTGTGTGAtttatgtt                                                                                           >  2:51010/1‑136 (MQ=255)
                                                     caGTTATATCAAGAGTTTAGGGGAGAATCATCATTGAGCAACTCAAAATCTATAACTTATTAGTAGAAAAGAAAATAACCAAAATATAAATAAGAAATAGTATCAAAAATTATATAAACTAATTTGAGCGATTTCGGTTgg                                                                                         >  2:740784/1‑140 (MQ=255)
                                                                  ggTTTAGGGCACTTATGCAACTGAGCAACTCAAAATCTATAACTTATTAGTAGAAAAGTAAATAACCAAAATATAAATAAGAAATAGTATCAAAAATTATATAAACTAATTTGTGTGATTTCGGTTGCATAGTTGCAAAAg                                                                            <  2:230517/141‑1 (MQ=255)
                                                                    tttAGGGCACTTATGCAACTGAGCAACTCAAAATCTATAACTTATTAGTAGAAAAGTAAATAACCAAAATATAAATAAGAAATAGTATCAAAAATTATATAAACTAATTTGTGTGATTTCGGTTGCATAGTTGCAAAAGTg                                                                          <  2:291331/141‑1 (MQ=255)
                                                                    ttgaagtcACTTAAGCAACTGAGCAACTCAAAATCTATAACTTATTAGTAGAAAAGTAAATAACCAAAATATAAATAAGAAAAAAAAAAAAAAATTATATAAACTAATTTGTGTGATTTCGGTTGCATAGTTGCAAAAGTg                                                                          <  2:399258/134‑1 (MQ=255)
                                                                      tAGGGCACTTATGCAACTGAGCAAGTCAAAATCTATAACTTATTAGTAGAAAAGTAAATAACCAAAATATAAATAAGAAATAGTATCAAAAATTATATAAACTAATTTGTGTGATTTCGGTTGCATAGTTGCAAAAGTGGa                                                                        <  2:283427/141‑1 (MQ=255)
                                                                      tAGGGCACTTATGCAACTGAGCAACTCAAAATCTATAACTTATTAGTAGAAAAGTAAATAACCAAAATATAAATAAGAAATAGTATCAAAAATTATATAAACTAATTTGTGTGATTTCGGTTGCATAGTTGCAAAAGTGGa                                                                        >  2:497487/1‑141 (MQ=255)
                                                                      tAGGGCACTTATGCAACTGAGCAACTCAAAATCTATAACTTATTAGTAGAAAAGTAAATAACCAAAATATAAATAAGAAATAGTATCAAAAATTATATAAACTAATTTGTGTGATTTCGGTTGAATAGTTGCAAAAGTGGa                                                                        >  2:124820/1‑141 (MQ=255)
                                                                            aCTTATGCAACTGAGCAACTCAAAATCTAGAACTTAATAGGAGAAAGGTAAATAACTTAAATATAAATAAGAAATAGGATCAAGATTTATATAAACTAATTTGTGTGATTTCGGTTGCATAGTTGCAAAAGTGGAtgaccc                                                                  <  2:302455/141‑6 (MQ=255)
                                                                              ttATGCAACTGATCAACTCAAAATCTATAACTTCTTAGTAGAAAAGTAAATAACCAAAATATAAATAAGAAATAGTATCAATAATTATATAAACTAATTTGTGTGATTTCGGTTGCATAGTTGCAAAAGTGGATTAAcccc                                                                <  2:453634/141‑1 (MQ=255)
                                                                              ttATGCAACTGAGCAACTCAAAATCTATAACTTATTAGTAGAAAAGTAAATAACCAAAATATAAATAAGAAATAGTATCAAAAATTATATAAACTAATTTGTGTGATTTCGGTTGCATAGTTGCAAAAGTGGATTAAcccc                                                                <  1:577302/141‑1 (MQ=255)
                                                                               tATGCAACTGAGCAACTCAAAATCTATAACTTACTAGTAGAAAAGTAAATAACTATAATATAAATAAGAAATAGTATCAAAAATTATATAAACTAATTTGTGTGATTTCGGTTGCATAGTTGCAAAAGTGGATTAACCCCt                                                               <  2:444340/141‑1 (MQ=255)
                                                                                aTGCAACTGAGCAACTCAAAATCTATAACTTATTAGTAGAAAAGTAAATAACCAAAATATAAATAAGAAATAGTATCAAAAATTATATAAACTAATTTGTGTGATTTCGGTTGCATAGTTGCAAAAGTGGATTAACCCCtt                                                              >  1:777433/1‑141 (MQ=255)
                                                                                    aaCTGAGCAACTCAAAATCTATAACTTATTAGTAGAAAAGTAAATAACCAAAATATAAATAAGAAATAGTATCAAAAATTATATAAACTAATTTGTGTGATTTCGGTTGCGTAGTTGCAAAAGTGGATTAACCCCTTGTGa                                                          >  1:172288/1‑141 (MQ=255)
                                                                                     aCTGAGCAACTCAAAATCTATAACTTATTAGTAGAAAAGTAAATAACCAAAATATAAATAAGAAATAGTATCAAAAATTATATATACTAATTTGTGTGATTTCGGTTGCATAGTTGCAAAAGTGGATTAACCCCTTGTGAc                                                         >  1:656786/1‑141 (MQ=255)
                                                                                     aCTGAGCAACTCAAAATCTATAACTTATTAGTAGAAAAGTAAATAACCAAAATATAAATAAGAAATAGTATCAAAAATTATATAAACTAATTTGTGTGATTTCGGTTGCATAGTTGCAAAAGTGGATTAACCCCTtgtgcc                                                         >  1:87922/1‑139 (MQ=255)
                                                                                     aCTGAGCAACTCAAAATCTATAACTTATTAGTAGAAAAGTAAATAACCAAAATATAAATAAGAAATAGTATCAAAAATTATATAAACTAATTTGTGTGATTTCGGTTGCATAGTTGCAAAAGTGGATTAACCCCTTGTGaa                                                         >  2:212571/1‑140 (MQ=255)
                                                                                             agtaaaaaTCTATAACTTATTAGTAGAAAAGTAAATAACCACAAAATCAAAGAAAAATAGTATCAAAAACTATATAAACTAATTTGTGGGATTTCGGTTGCATAGTTGCAAAAGTGGATTAACCCCTTGTGACAGTAAGaa                                                 <  1:152299/137‑1 (MQ=255)
                                                                                             acccAAAATCTAGAACTTATTAGTAGAAAAGTAAATAACCAAAACATCAACAATAAAGAGTATCAAGAATTATATAAACTAATTTGTGTGATTTCGGTTGCATAGTTGCAAAAGTGGATTAACCCCTTGTGACAGTAAGaa                                                 <  1:106857/138‑1 (MQ=255)
                                                                                              cTCAAAATCTATAACTTATTAGTAGAAAAGTAAATAACCAAAATATAAATAAGAAATAGTATCAAAAATTATATAAACTAATTTGTGTGATTTCGGTTGCATAGTTGCAAAAGTGGATTAACCCCTTGTGACAGTAAGaaa                                                >  2:377193/1‑141 (MQ=255)
                                                                                                 aaaaTCTATAACTTATTAGTAGAAAAGTAAATAACCAAAATATAAATAAGAAATAGTATCAAAAATTATATAAACTAATTTGTGTGATTTCGGTTGCATAGTTGCAAAAGTGGATTAACCCCTTGTGACAGTAAGaaaaaa                                             <  1:506602/141‑1 (MQ=255)
                                                                                                 aaaaTCTATAACTTATTAGTAGAAAACTAAATAACCAAAATATAAATAAGAAATAGTATCAAAAATTATATAAACTAATTTGTGTGATTTCGGTTGCATAGTTGCAAAAGTGGATTAACCGCTTGTGACAGTAAGaaaaaa                                             <  1:489650/141‑1 (MQ=255)
                                                                                                       tataACTTATTAGTAGAAAAGTAAATAACCAAAATATGAATAAGAAATAGTATCAAAAATTATATAAACTAATTTGTGTGATTTCGGTTGCATAGTTGCAAAAGTGGATTAACCCCTTGTGACAGTAAGAAAAAAATTGCa                                       <  1:136546/141‑1 (MQ=255)
                                                                                                       tataACTTATTAGTAGAAAAGTAAATAACCAAAATATGAATAAGAAATAGTATCAAAAATTATATAAACTAATTTGTGTGATTTCGGTTGCATAGTTGCAAAAGTGGATTAACCCCTTGTGACAGTAAGAAAAAAATTGCa                                       <  2:790269/141‑1 (MQ=255)
                                                                                                       tataACTTATTAGTAGAAAAGTAAATAACCAAAATATAAATAAGAAATAGTATCAAAAATTATATAAACTAATTTGTGTGATTTCGGTTGCATAGTTGCAAAAGTGGATTAACCCCTTGTGACAGTAAGAAAAAAATTGCa                                       >  1:132721/1‑141 (MQ=255)
                                                                                                       tataACTTATTAGTAGAAAAGTAAATAACCAAAATATAAATAAGAAATAGTATCAAAAATTATATAAACTAAATTGTGTGATTTCGGTTGCATAGTTGCAAAAGTGGATTAACCCCTCGTGACAGTAAGAAAAAAATTgaa                                       >  2:764118/1‑139 (MQ=255)
                                                                                                        ataACTTATTAGTAGAAAAGTAAATAACCAAAATATAAATAAGAAATAGTATCAAAAATTATATAAACTAATTTGTGTGATTTCGGTTGCATAGTTGCAAAAGTGGATTAACCCCTTGTGACAGTAAGAAAAAAATTGCaa                                      >  1:300079/1‑141 (MQ=255)
                                                                                                            cTTATTAGTAGAAAAGTAAATAACCAAAATATAAATAAGAAATAGTATCAAAAATTATATAAACTAATTTGTGTGATTTCGGTTGCATAGTTGCAAAAGTGGATTAACCCCTTGTGACAGTAAGAAAAAAATTGCAACTgg                                  >  2:98121/1‑141 (MQ=255)
                                                                                                              tattaGTAGAAAAGTAAATAACCAAAATATAAATAAGAAATAGTATCAAAAATTATATAAACTAATTTGTGTGATTTCGGTTGCATAGTTGCAAAAGTGGATTAACCCCTTGTGACAGTAAGAAAAAAATTGCAACTGGaa                                <  1:239124/141‑1 (MQ=255)
                                                                                                                       aaaaGTAAATAACCAAAATATAAATAAGAAATAGTATCAAAAATTATATAAACTAATTTGTGTGATTTCGGTTGCATAGTTGCAAAAGTGGATTAACCCCTTGTGACAGTAAGAAAAAAATTGCAACTGGAAAATTTaaaa                       <  1:73621/141‑1 (MQ=255)
                                                                                                                            tAAATAACCAAAATATAAATGAGAAATAGTATCAAAAATTATATCAACTAATTTGTGTGATTTCGGTTGCATAGTTGCAAAAGTGAATTAACCCCTTGTGACAGTAAGAAAAAAATTGCAACTGGAAAATTTAAAACGAGt                  >  2:444952/1‑141 (MQ=255)
                                                                                                                            tAAATAACCAAAATATAAATAAGAAATAGTATCAAAAATTATATAAACTAATTTGTGTGATTTCGGTTGCATAGTTGCAAAAGTGGATTAACCCCTTGTGACAGTAAGAAAAAAATTGCAACTGGAAAATTTAAAACGAGt                  >  1:439152/1‑141 (MQ=255)
                                                                                                                            tAAATAACCAAAATATAAATAAGAAATAGTATCAAAAATTATATAAACTAATTTGTGTGATTTCGGTTGCATAGTTGCAAAAGTGGATTAACCCCTTGTGACAGTAAGAAAAAAATTGCAACTGGAAAATTTAAAACGAGt                  >  1:767585/1‑141 (MQ=255)
                                                                                                                              aaaatccAATATATAAATAAGAAATAGTATCAAATATTTTATAGATTAATTTGTGTGATTTGTGTTGCTTAGTGGCAAAAGTGTATTAATTACTTGTTACAGTAATAAAAAAAGTGGATCTGGAAAATTTAATGCGATTTg                <  2:345806/136‑1 (MQ=255)
                                                                                                                               aTAACCAAAATATAAATAAGAAATAGTATCAAAAATTATATAAACTAATTTGTGTGATTTCGGTTGCATAGTTGCAAAAGTGGATTAACCCCTTGTGACAGGAAGAAAAAAATTGCAACTGGAAAATTTAAAACGAGTTGc               >  1:349380/1‑141 (MQ=255)
                                                                                                                               aTAACCAAAATATAAATAAGAAATAGTATCAAAAATTATATAAACTAATTTGTGTGATTTCGGTTGCATAGTTGCAAAAGAGGATTAACCCCTTGTGACAGTAAGAAAAAAATTGCAACTGGAAAATTTAAAAAGAGTTGc               >  1:418964/1‑141 (MQ=255)
                                                                                                                                 aaCCAAAATATAAATAAGAAATAGTATCAAAAATTATATAAACTAATTTGTGTGATTTCGGTTGCATAGTTGCAAAAGTGGATTAACCCCTTGTGACAGTAAGAAAAAAATTGCAACTGGAAAATTTAAAACGAGGTGCAt             >  1:201760/1‑141 (MQ=255)
                                                                                                                                  aCCAAAATATAAATAAGAAATAGTATCAAAAATTATATAAACTAATTTGTGTGATTTCGGTTGCATAGTTGCAAAAGTGGATTAACCCCTTGTGACAGTAAGAAAAAAATTGCAACTGGAAAATTTAAAACGAGTTGCATa            >  2:424607/1‑141 (MQ=255)
                                                                                                                                     aaaaTATAAATAAGAAATAGTATCAAAAATTATATAAACTAATTTGTGTGATTTCGGTTGCATAGTTGCAAAAGTGGATTAACCCCTTGTGACAGTAAGAAAAAAATTGCAACTGGAAAATTTAAAACGAGTTGCATAGCt         >  1:21071/1‑141 (MQ=255)
                                                                                                                                        atatAAATAAGAAATAGTATCAAAAATTATATAAACTAATTTGTGTGATTTCGGTTGCATAGTTGCAAAAGTGGATTAACCCCTTGTGACAGTAAGAAAAAAATTGCAACTGGAAAATTTAAAACGAGTTGCATAGCTGTa      >  1:142077/1‑141 (MQ=255)
                                                                                                                                         tataAATAAGAAATAGTATCAAAAATTATATAAACTAATTTGTGTGATTTCGGTTGCATAGTTGCAAAAGTGGATTAACCCCTTGTGACAGTAAGAAAAAAATTGCAACTGGAAAATTTAAAACGAGTTGCATAGCTGTaa     <  1:75227/141‑1 (MQ=255)
                                                                                                                                         tataAATAAGAAATAGTATCAAAAATTATATAAACTAATTTGTGTGATTTCGGTTGCATAGTTGCAAAAGTGGATTAACCCCTTGTGACAGTAAGAAAAAAATTGCAACTGGAAAATTTAAAACGAGTTGCATAGCTGTaa     <  1:321808/141‑1 (MQ=255)
                                                                                                                                         aaaaaataaGAAATAGTATAAAAAATTATATAAACTAATTTGTGTGATTTCGGTTGCATAGTTGCAAAAGTGGATTAACCCCTTGTAAAAGAAAAAAAAAAATTGCAACTGGAAAATTTAAAACGAGTTGCATAGCTGTaa     <  2:510952/138‑1 (MQ=255)
                                                                                                                                            aaataaGAAATAGTATCAAAAATTATATAAACTAATTTGTGTGATTTCGGTTGCATAGTTGCAAAAGTGGATTAACCCCTTGTGACAGTAAGAAAAAAATTGCAACTGGAAAATTTAAAACGAGTTGCATAGCTGTAAAtt  <  1:98121/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                            
CGAATGCAACCGAATGCAACCGAAAATTTTAAAACGGGTTGCACCTATAATCACAGTTATATCAAGGGTTTAGGGCACTTATGCAACTGAGCAACTCAAAATCTATAACTTATTAGTAGAAAAGTAAATAACCAAAATATAAATAAGAAATAGTATCAAAAATTATATAAACTAATTTGTGTGATTTCGGTTGCATAGTTGCAAAAGTGGATTAACCCCTTGTGACAGTAAGAAAAAAATTGCAACTGGAAAATTTAAAACGAGTTGCATAGCTGTAAATT  >  CP000730/903733‑904013

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: