Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	1,677,731	A→G	5.5%	E290G (GAG→GGG)	glyS →	glycine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,677,731	0	A	G	5.5%	240.9 / 3.5	89	E290G (GAG→GGG)	glyS	glycine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base A (41/41);  new base G (2/3);  total (44/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.16e-01

TGCAAGTGACTGGTTAACAAGCTTAAATATGAGCAGTGAAAATATGCGTTTACGTGATCATGATGAAGATGAATTATCTCATTACTCAAATGCAACAACTGATATTGAATATAAATTCCCATTTGGTTGGGGTGAGTTATGGGGTATCGCAAGTCGTACAGACTTCGACTTACGTAAACATGCTGAACACTCTGGTGAAGATTTCAGATACCATGATCCAGAAACGAACGAAAAATATATTCCATATTGTATCGAGCCATCACTTGGTGC  >  CP000730/1677597‑1677866                                                                                                                                       |                                                                                                                                        tGCAAGTGACTGGTTAACAATCTTAAATATGAGCAGTGAAAATATGCGTTTACGTGATCATGATGAAGATGAATTATCTCATTACTCAAATGCAACAACTGATATTGAATATAAATTCCCATTTGGTTGGGGTGAGTTATg                                                                                                                                   <  1:340080/141‑1 (MQ=255)   cAAGTGACTGGTTAACAAGCTTAAATATGAGCAGTGAAAATATGCGTTTACGTGATCATGATGAAGATGAATTATCTCATTACTCAAATGCAACAACTGATATTGAATATAAATTCCCATTTGGTTGGGGTGAGTTATggg                                                                                                                                 >  1:805440/1‑141 (MQ=255)   cAAGTGACTGGTTAACAAGCTTAAATATGAGCAGTGAAAATATGCGTTTACGTGATCATGATGAAGATGAATTATCTCATTACTCAAATGCAACAACTGATATTGAATATAAATTCCCATTTGGTTGGGGTGAGTTATggg                                                                                                                                 >  1:302420/1‑141 (MQ=255)      tttaCTGGTTAACAATCTTAAATATGAGCAGTGAAAATATGCGGTTACGTGAGCATGATGAAGATGAATTATCTCATTACTCAAATGCAACAACTGATATTGAATATAAATTCCCATTTGGTTGGGGTGAGTTATGGGGTa                                                                                                                              <  2:139013/138‑1 (MQ=255)      gTGACTTGTTAACTAGCTTAAATATGAGCAGTGAAAATATGCGTTTACGTGATCATGATGAAGATGAATTATCTCATTACTCAAATGCAACAACTGATATTGAATATAAATTCCCATTTGGTTGGGGTGAGTTATGGGGTa                                                                                                                              <  1:416515/141‑1 (MQ=255)      gTGACTGGTTAATAAGCTTAAATATGAGCAGTGAAAATATGCGTTTACGTGATCATGATGAAGATGAATTATCTCATTACTCAAATGCAACAACTGATATTGAATATAAATTCCCATTTGGTTGGGGTGAGTTATGGGGTa                                                                                                                              <  1:454464/141‑1 (MQ=255)       tGACTGGTTAACAAGCTTAAATATGAGCAGTGAAAATATGCGTTTACGTGATCATGATGAAGATGAATTATCTCATTACTCAAATGCAACAACTGATATTGAATATAAATTCCCATTTGGTTGGGGTGGGTTATGGTGTAt                                                                                                                             >  1:43343/1‑141 (MQ=255)       tGACTGGTTAACAAGCTTAAATATGAGCAGTGAAAATATGCGTTTACGTGATCATGATGAAGATGAATTATCTCATTACTCAAATGCAACAACTGATATTGAATATAAATTCCCATTTGGTTGGGGTGGGTTATGGGGTAt                                                                                                                             >  1:444596/1‑141 (MQ=255)       tGACTGGTTAACAAGCTTAAATATGAGCAGTGAAAATATGCGTTTACGTGATCATGATGAAGATGAATTATCTCATTACTCAAATGCAACAACTGATATTGAATATAAATTCCCATTTGGTTGGGGTGAGTTATGGGGTAt                                                                                                                             <  1:494965/141‑1 (MQ=255)       tGACTGGTTAACAAGCTTAAATATGAGCAGTGAAAATATGCGTTTACGTGATCATGATGAAGATGAATTATCTCATTACTCAAATGCAACAACTGATATTGAATATAAATTCCCATTTGGTTGGGGTGAGTTATGGGGTAt                                                                                                                             >  1:436085/1‑141 (MQ=255)       tGACTGGTTAACAAGCTTAAATATGAGCAGTGAAAATATGCGTTTACGTGATCATGATGAAGATGAATTATCTCATTACTCAAATGCAACAACTGATATTGAATATAAATTCCCATTTGGTTGGGGTGAGTTATGGGGTAt                                                                                                                             >  2:753041/1‑141 (MQ=255)       tGACTGGTTAACAAGCTTAAATATGAGCAGTGAAAATATGCGTTTACGTGATCATGATGAAGATGAATTATCTCATTACTCAAATGCAACAACTGATATTGAATATAAATTCCCATTTGGTTGGGGTGAGTTATGGGGTAt                                                                                                                             <  2:787958/141‑1 (MQ=255)         aCTGGTTAACAAGCTTAAATATGAGCAGTGAAAATATGCGTTTACGTGATCATGATGAAGATGAATTATCTCATTACTCAAATGCAACAACTGATATTGAATATAAATTCCCATTTGGTTGGGGTGAGTTATGGGGTATCg                                                                                                                           <  2:436090/141‑1 (MQ=255)                      cTTAAATATGAGCAGTGAAAATATGCGTTTACGTGATCATGATGAAGATGGATTATGTCATTACTCAAATGCAACAACTGATATTGAATATAAATTCCCATTTGGTTGGGGTGAGTTATGGGGTATCGCAAGTCGTACAGa                                                                                                              >  1:724382/1‑141 (MQ=255)                      cTTAAATATGAGCAGTGAAAATATGCGTTTACGTGATCATGATGAAGATGAATTATCTCATTACTCAAATGCAACAACTGATATTGAATATAAATTCCCATTTGGTTGGGGTGAGTTATGGGGTATCGCAAGTCGTACAgg                                                                                                              >  1:805811/1‑140 (MQ=255)                         aaaTATGAGCAGTGAAAATATGCGTTTACGTGATCATGATGAAGATGAATTATCTCATTACTCAAATGCAACAACTGATATTGAATATAAATTCCCATTTGGTTGGGGTGAGTTATGGGGTATCGCAAGTCGTACAGACtt                                                                                                           <  1:335855/141‑1 (MQ=255)                         aaaTATGAGCAGTGAAAATATGCGTTTACGTGATCATGATGAAGATGAATTATCTCATTACTCAAATGCAACAACTGATATTGAATATAAATTCCCATTTGGATGGGGTGAGTTATGGGGTATCGCGAGTCGTACAGACtt                                                                                                           >  1:734772/1‑141 (MQ=255)                          aaTATGAGCAGTGCAAATATGCGTTTACGTGATCATGATGAAGATGAATTAACTCATTACTCAAATGCAACAACTGATATTGAATATAAATCCCCATTTGGTTGGGGTGAGTTATGGGGTATCGCAAGTCGTACAGACTTc                                                                                                          >  2:575129/1‑141 (MQ=255)                          aaTATGAGCAGTGAAAATATGCGTTTACGTGATCATGATGAAGATGAATTATCTCATTACTCAAATGCAACAACTGATATTGAATATAAATTCCCATTTGGTTGGGGTGAGTTATGGGGTATCGCAAGTCGTACAGACTTc                                                                                                          >  2:222589/1‑141 (MQ=255)                          aaTATGAGCAGTGAAAATATGCGTTTACGTGATCATGATGAAGATGAATTATCTCATTACTCAAATGCAACAACTGATATTGAATATAAATTCCCATTTGGTTGGGGTGAGTTATGGGGTATCGCAAGTCGTACAGACTTc                                                                                                          >  2:586548/1‑141 (MQ=255)                          aaTATGAGCAGTGAAAATATGCGTTTACGTGATCATGATGAAGATGAATTATCTCATTACTCAAATGCAACAACTGATATTGAATATAAATTCCCATTTGGTTGGGGTGAGTTATGGGGTATCGCAAGTAGTACAGACTTc                                                                                                          >  2:768012/1‑141 (MQ=255)                                aGCAGGGAAAAGCTGCCTTTACGTGATCATGATGAAGATGAATTATCTCATTACTCAAATGCAACAACTGATATTGAATATAAATTCCCAGTTGGTTGGGGTGAGTTATGGGGTATCGCAAGTCGTACAGACCTCGACTTa                                                                                                    <  1:42172/141‑1 (MQ=255)                                 gCAGTGAAAATATGCGTTTACGTGATCATGATGAAGATGCATTATATGGATATTAAAAGGTAACAACAAGTCGAAATTATAAATTCCCATTATGTTGAGGGGTGGTTAGGGGTAGCGCAAGGCGTACATATTTCGACTTAc                                                                                                   >  1:796693/1‑141 (MQ=255)                                 gCAGTGAAAATATGCGTTTACGTGATCATGATGAAGATGAATTATCTCATTACTCAAATGCAACAACTGATATTGAATATAAATTCCCATTTGGTTGGGGTGAGTTATGGGGTATCGCAAGTCGTACAGACTTCGACTTAc                                                                                                   <  2:535852/141‑1 (MQ=255)                                  cAGTGAAAATATGCGTTTACGTGATCATGATGAAGATGAATTATCTCATTACTCAAATGCAACAACTGATATTGAATATAAATTCCCATTTGGTTGGGGTGAGTTATGGGGTATCGCAAGTCGTACAGACTTCGACTTACg                                                                                                  >  2:40053/1‑141 (MQ=255)                                   aGTGAAAATATGCGTTTACGTGATCATGATGAAGATGAATTATCTCATTACTCAAATGCAACAACTGATATTGAATATAAATTCCCATTTGGTTGGGGTGAGTTATGGGGTATCGCAAGTCGTACAGACTTCGACTTACGt                                                                                                 <  1:365058/141‑1 (MQ=255)                                           tatGCGTTTACGTGATCATGATGAAGATGAATTATCTCATTACTCAAATGCAACAACTGATATTGAATATAAATTCCAATTTGGTTGGGGTGAGTTATGGGGTATCGCAAGTCGTACAGACTTCGACTTACGTAAACATGc                                                                                         >  1:32282/1‑141 (MQ=255)                                             tGCGTTTACGTGATCATGATGAAGATGAATTATCTCATTACTCAAATGCAACAACTGATATTGCATATAAATTCCCATTTGGTTGGGGTGAGTTATGGGGTATCGCAAGTCGTACAGACTTCGACTTACGTAAACATGCTg                                                                                       <  1:545840/141‑1 (MQ=255)                                                  ttACGTGATCATGATGAAGATGAATTATCTCATTACTCAAATGCAACAACTGATATTGAATATAAATTCCCATTTGGTTGGGGTGAGTTATGGGGTATCGCAAGTCGTACTGGATTTGTGTTACGTAAACATGCTGAacac                                                                                  <  1:393294/141‑1 (MQ=255)                                                         aTCATGATGAAGATGAATTATCTCATTACTCAAATGCAACAACTGATATTGAATATAAATTCCCATTTGGTTGGGGTGAGTTATGGGGTATCGCAAGTCGTACAGACTTCGACTTACGTAAACATGCTGAACACTCTGGTg                                                                           <  2:805440/141‑1 (MQ=255)                                                         aTCATGATGAAGATGAATTATCTCATTACTCAAATGCAACAACTGATATTGAATATAAATTCCCATTTGGTTGGGGTGAGTTATGGGGTATCGCAAGTCGTACAGACTTCGACTTACGTAAACATGCTGAACACTCTGGTg                                                                           <  2:302420/141‑1 (MQ=255)                                                         aTCATGATGAAGATGAATTATCTCATTACGAAAATGCAACAACTGATATTGAATAGAAATTCCCATTTGGTTGGGGTGGGTTATGGGGTATCGCAAGTAGTACGGACTTCGACTTACGTAAACATGCTGAACACTCTCGTg                                                                           <  2:160245/141‑1 (MQ=255)                                                              gatgaagatgaaTTATCTCATTACTCAAATGCAACAACTGATATTGAATATAAATTCCCATTTGGTTGGGGTGAGTTATGGGGTATCGCAAGTCATACAGACTTCGACTTACGTAAACATGCTGAACACTCTGGTGAAGAt                                                                      >  1:616007/1‑141 (MQ=255)                                                               atgaagatgaaTTATCTCATTACTCAAATGCAACAACTGATATTGAATATAAATTCCCATTTGGTTAGGGTGAGTTATGGGGTATCGCAAGTCGTACAGACTTCGACTTACGTAAACATGCTGAACACTCTGGTGAAGAtt                                                                     <  2:271126/141‑1 (MQ=255)                                                                      tgaaTTATCTCATTACTCAAATGCAACAACTGATATTGAATATAAATTCCCATTTGGTTGGGGTGAGTTATGGGGTATCGCAAGTCGTACAGACTTCGACTTACGTAAACATGCTGAACACTCTGGTGAAGATTTCAGATa                                                              >  1:530007/1‑141 (MQ=255)                                                                        aactagcacaatACTCAAATGCAAAAACTGATATTTTATATAAATTTCCATGTGGTGGGGGTTAGTTATGGGGTATAGCAAGTCGTACAGACTTCGACGTACGTAAACATGCTGACCACGCTGGTGAAGATTTCAGATAcc                                                            <  2:285072/130‑1 (MQ=255)                                                                        aaatATCTCATTCCTCAGATACAACAACTGATATTGAATATAAATTCCCATTTGGTGGGTGTCAGTTATGGTGTATCGCAATTCGTACAGACTTCGACTTACGTAAACATGCTGAACACTCTGGTGAAGATTTCAGATAcc                                                            <  2:456415/138‑1 (MQ=255)                                                                        aaTTATCTCATTACTCAAATGCAACAACTGATATTGAATATAAATTCCCATTTGGTTGGGGTGAGTTATGGGGTATCGCAAGTCGTACAGACTTCGACTTACGTAAACATGCTGAACACTCTGGTGAAGATTTCAGATAcc                                                            <  1:713786/141‑1 (MQ=255)                                                                         aTTATCTCATTACTCAAATGCAACAACTGATATTGAATATAAATTCCCATTTGGTTGGGGTGAGTTATGGGGTATCGCAAGTCGTACAGACTTCGACTTACGTAAACATGCTGAACACTCTGGTGAAGATTTCAGATACCa                                                           <  2:677748/141‑1 (MQ=255)                                                                          ttATCTCATTACTCAAATGCAACAACTGATATTGAATATAAATTCCCATTTGGTTGGGGTGAGTTATGGGGTATCGCAAGTCGTACAGACTTGGACTTACGTAAACATGCTGAACACTCTGGTGAAGATTTCAGATAccct                                                          >  1:212174/1‑139 (MQ=255)                                                                          ttATCTCATTACTCAAATGCAACAACTGATATTGAATATAAATTCCCATTTGGTTGGGGTGAGTTATGGGGTATCGCAAGTCGTACAGACTTCGACTTACGTAAACATGCTGAACACTCTGGTGAAGATTTCAGATACCAt                                                          >  1:694887/1‑141 (MQ=255)                                                                          ttATCTCATTACTCAAATGCAACAACTGATATTGAATATAAATTCCCATTTGGTTGGGGTGAGTTATGGGGTATCGCAAGTCGTACAGACTTCGACTTACGTAAACATGCTGAACACTCTGGTGAAGATTTCAGATACCAt                                                          <  1:40053/141‑1 (MQ=255)                                                                          ttATCTCAGTACTCAAATGCAACAACTGATATTGAATATAAATTCTCATTTGGTTGGGGGGAGTTATGGGGTATCGCAAGTCGTACAGACTTCGACTTACGTAAACATGCTGAACACTCTGGTGAAGATTTCAGATACCAt                                                          <  1:526913/141‑1 (MQ=255)                                                                           tATCTCATTACTCAAATGCAACAACTGATATTGAATATAAATTCCCATTTGGTTGGTGTGAGTTATGGGGTATCGCAAGTCGTACAGACTTCGACTTACGTAAACATGCTGAACACTCTGGTGAAGATTTCAGATACCATg                                                         <  2:262806/141‑1 (MQ=255)                                                                             tctcATTACTCAAATGCAACAACTGATATTGAATATAAATTCCCATTTGGTTGGGGTGAGTTATGGGGTATCGCAAGTCGTACAGACTTCGACTTACGTAAACATGCTGAACACTCTGGTGAAGATTTCAGATACCATGAt                                                       <  1:732415/141‑1 (MQ=255)                                                                                    ccTCAAAGGCAACCACTGATATTGAAGATAAAAACACTTTTGGTTGGGGTGTGTTATGGGGTATCGCAAGTCGTACAGACTTCGACTTACGTAAACATGCTGAACACTCTGGTGAATATTTCAGATACCATGATCCAGaaa                                                <  2:443176/140‑1 (MQ=255)                                                                                    aCTCAAATGCAACAACTGATATTGAATATAAATTCCCATTTGGTTGGGGTGGGTTATGGGGTATCGCAAGTCGTACAGACTTCGACTTACGTAAACATGCTGAACACTCTGGTGAAGATTTCAGATACCATGATCCAGaaa                                                <  2:43343/141‑1 (MQ=255)                                                                                    aCTCAAATGCAACAACTGATATTGAATATAAATTCCCATTTGGTTGGGGTGGGTTATGGGGTATCGCAAGTCGTACAGACTTCGACTTACGTAAACATGCTGAACACTCTGGTGAAGATTTCAGATACCATGATCCAGaaa                                                <  2:444596/141‑1 (MQ=255)                                                                                    aCTCAAATGCAACAACTGATATTGAATATAAATTCCCATTTGGTTGGGGTGAGTTATGGGGTATCGCAAGTCGTACAGACTTCGACTTACGTAAACATGCTGAACACTCTGGTGAAGATTTCAGATACCATGATCCAGaaa                                                <  2:436085/141‑1 (MQ=255)                                                                                    aCTCAAATGCAACAACTGATATTGAATATAAATTCCCATTTGGTGGGGGTGAGTTATGGGGTATCGCAAGTCGTACAGACTTCGACTTACGTAAACATGCTGAACACTCTGGTGAAGATTTCAGATACCATGATCCAGaaa                                                <  1:753041/141‑1 (MQ=255)                                                                                           tGCAACAACTGATATTGAATATAAATTCCCATTTGGTTGGGGTGAGTTATGGGGTATCGCAAGTCGTACAGACTTCGACTTACGTAAACATGCTGAACACTCTGGTGAAGATTTCAGATACCATGATCCAGAAacgaacga                                         <  2:530007/141‑1 (MQ=255)                                                                                            gCAACAACTGATATTGAATATAAATTCCCATTTGGTTGGGGTGAGTTATGGGGTATCGCAAGTCGTACAGACTTCGACTTACGTAAACATGCTGAACACTCTGGTGAAGATTTCAGATACCATGATCCAGAAAcgaacgaa                                        <  2:757929/141‑1 (MQ=255)                                                                                            gCAACAACTGATATTGAATATAAATTCCCATTTGGTTGGGGTGAGTTATGGGGTATCGCAAGTCGTACAGACTTCGACTTACGTAAACATGCTGAACACTCTGGTGAAGATTTCAGATACCATGATCCAGAAAcgaacgaa                                        <  2:283318/141‑1 (MQ=255)                                                                                              aacaacTGATATTGAATATAAATTCCCATTTGGTAGGGGTGAGTTATGGGGTATCGCAAGTCGTACAGACTTCGACTTACGTAAACATGCTGAACACTCTGGTGAAGATTTCAGATACCATGATCCAGAAACGAACGacaa                                      >  2:375937/1‑141 (MQ=255)                                                                                               ataacTGATGTTGTATATAAAGTCCGATTTGGTTGGGGTGAGTTATGGGGTATCGCAAGTCGTACAGACTTCGACTTACGTAAACATGCTGAACACTCTGGTGAAGATTTCAGATACCATGATCCAGAACCGAACGaaaaa                                     >  2:73492/3‑141 (MQ=255)                                                                                               acaacTGATATTGAATATAAATTCCCATTTGGTTGGGGTGAGTTATGGGGTATCGCAAGTCGTACAGACTTCGACTTACGTAAACATGCTGAACACTCTGGTGAAGATTTCAGATACCATGATCCAGAAACGAACGaaaaa                                     >  1:784939/1‑141 (MQ=255)                                                                                                       tatTGAATATAAATTCCCATTTGGTTGGGGTGAGTTATGGGGTATCGCAAGTCGTACAGACTTCGACTTACGTAAACATGCTGAACACTCTGGTGAAGATTTCAGATACCATGATCCAGAACCGACCGAAAAATATATTcc                             >  2:188060/1‑141 (MQ=255)                                                                                                         ttGAATATAAATTCCCATTTGGTTGGGGTGAGTTATGGGGTATCGCAAGTCGTACAGACTTCGACTTACGTAAACATGCTGAACACTCTGGTGAAGATTTCAGATACCATGATCCAGAAACGAACGAAAAATATATTCCat                           >  1:6855/1‑141 (MQ=255)                                                                                                          tGAATATAAATTCCCATTTGGTTGGGGTGAGTTATGGGGTATCGCAAGTCGTACAGACTTCGACTTACGTAAACATGCTGAACACTCTGGTGAAGATTTCAGATACCATGATCCAGAAACGAACGAAAAATATATTCCata                          <  1:265857/141‑1 (MQ=255)                                                                                                          tGAATATAAATTCCCATTTGGTTGGGGTGAGTTATGGGGTATCGCAAGTCGTACAGACTTCGACTTACGTAAACATGCTGAACACTCTGGTGAAGATTTCAGATACCATGATCCAGAAACGAACGAAAAATATATTCCata                          >  2:507038/1‑141 (MQ=255)                                                                                                          tGAATATAAATTCCCATTTGGTTGGGGTGAGTTATGGGGTATCGCAAGTCGTACAGACTTCGACTTACGTAAACATGCTGAACACTCTGGTGAAGATTTCAGATACCATGATCCAGAAACGAACGAAAAATATATTCCata                          <  2:242603/141‑1 (MQ=255)                                                                                                          tGAATATAAATTCCCATTTGGTTGGGGTGAGTTATGGGGTATCGCAAGTCGTACAGACTTCGACTTACGTAAACATGCTGAACACTCTGGTGAAGATTTCAGATACCATGATCCAGAAACGAACGAAAAATATATTCCata                          <  2:455290/141‑1 (MQ=255)                                                                                                           gAATATAAATTCCCATTTGGTTGGGGTGAGTTATGGGGTATCGCAAGTCGTACAGACTTCGACTTACGTAAACATGCTGAACACTCTGGTGAAGATTTCAGATACCATGATCCAGAAACGAACGAAAAATATATTCCatat                         >  1:58835/1‑141 (MQ=255)                                                                                                           gAATATAAATTACCATTTGGTTGGGGTTAGTTATGGGGGATGGCAAGTCGTAGAGACTTCGACTTACGTAAACATGCTGAACACTCTGGTGAAGATTTCAGATACCATGATCCAGAAACGAACGAAAAATATATTCCatat                         >  2:473281/1‑141 (MQ=255)                                                                                                            aaTATAAATTCCCATTTGGTTGGGGTGAGTTATGGGGTATCGCAAGTCGTACAGACTTCGACTTACGTAAACATGCTGAACACTCTGGTGAAGATTTCAGATACCATGATCCAGAAACGAACGAAAAATATATTCCATAtt                        <  2:468075/141‑1 (MQ=255)                                                                                                             agaaaaaTTCCCATTTGGTTGGGGTGAGTTATGGGTCATCGCAAGTCGTACAGACTTCGACTTACGTAAACATGCTGAACACTCTGGTGAAGATTTCAGTTACCATGATCCAGAAACGAACGAAAAATATATTCCATATTg                       <  2:206846/137‑1 (MQ=255)                                                                                                               ataAATTCCCATTTGGTTGGGGTGAGTTATGGGGTATCGCAAGTCGTACAGACTTCGACTTACGTAAACATGCTGAACACTCTGGTGAAGATTTCAGATACCATGATCCAGAAACGAACGAAAAATATATTCCATATTGTa                     >  2:562393/1‑141 (MQ=255)                                                                                                               ataAATTCCCATTTGGTTGGGGTGAGTTATGGGGTATCGCAAGTCGTACAGACTTCGACTTACGTAAACATGCTGAACACTCTGGTGAAGATTTCAGATACCATGATCCAGAAACGAACGAAAAATATATTCCATATTGTa                     >  1:569875/1‑141 (MQ=255)                                                                                                                 aaaTTCCCATTTGGTTGGGGTGAGTTATGGGGTATCGCAAGTCGTACAGACTTCGACTTACGTAAACATGCTGAACACTCTGGTGAAGATTTCAGATACCATGATCCAGAAACGAACGAAAAATATATTCCATATTGTATc                   <  1:222589/141‑1 (MQ=255)                                                                                                                 aaaTCCCCATTTGGTTGGGGTGAGTTAGGGGGTATCGCAAGTCGTACAGACTTCGACTTACGTAAACATGCTGAACACTCTGGTGAAGATTTCAGATACCATGATCCAGAAACGAACGAAAAATATATTCCATATTGTATc                   <  1:575129/141‑1 (MQ=255)                                                                                                                   aTTCCCATTTGGTTGGGGTGATGTATGGGGTATCGCAAGTCGTACAGACTTCGACTTACGTAAACATGCTGAACACTCTGGTGAAGATTTCAGATACCATGATCCAGAAACGAACGAAAAATATATTCCATATTGTATCGa                 <  2:557388/141‑1 (MQ=255)                                                                                                                   aTTCCCATTTGGTTGGGGTGAGTTATGGGGTATCGCAAGTCGTACAGACTTCGACTTACGTAAACATGCTGAACACTCTGGTGAAGATTTCAGATACCATGATCCAGCAACGAACGAAAAATATATTCCATATTGTATCGa                 >  1:81784/1‑141 (MQ=255)                                                                                                                   aTTCCCATTTGGTTGGGGTGAGTTATGGGGTATCGCAAGTCGTACAGACTTCGACTTACGTAAACATGCTGAACACTCTGGTGAAGATTTCAGATACCATGATCCAGAAACGAACGAAAAATATATTCCATATTGTATCgg                 >  2:96215/1‑140 (MQ=255)                                                                                                                   aTTCCCATTTGGTTGGGGTGAGTTATGGGGTATCGCAAGTCGTACAGACTTCGACTTACGTAAACATGCTGAACACTCTGGTGAAGATTTCAGATACCATGATCCAGAAACGAACGAAAAATATATTCCATATTGTATCgg                 >  1:81717/1‑140 (MQ=255)                                                                                                                   aTTCCCATTTGGTTGGGGTGAGTTATGGGGTATCGCAAGTCGTACAGACTTCGACTTACGTAAACATACTGAACACCATGGTGAACATTTCAGATACCATGATCCAGAAACGAACGCAAAATCTATTACAAATTGTaccta                 >  2:287714/1‑137 (MQ=255)                                                                                                                         aTTTGGTTGGGGTGAGTTATGGGGTATCGCAAGTCGTACAGACTTCGACTTACGTAAACATGCTGAACACTCTGGTGAAGATTTCAGATACCATGATCCAGAAACGAACGAAAAATATATTCCATATTGTATCGAGCCATc           >  2:738537/1‑141 (MQ=255)                                                                                                                         aTTTGGTTGGGGTGAGTTATGGGGTATCGCAAGTCGTACAGACTTCGACTTACGTAAACATGCTGAACACTCTGGTGAAGATTTAAGATACCATGATCCAGAAACGAACGAAACATATATTCCATATTGTTTCGAGCCAta           >  2:673343/1‑140 (MQ=255)                                                                                                                            tggttggGGTGAGTTATGGGGTATCGCAAGTCGTACAGACTTCGACTTACGTAAACATGCTGAACACTCTGGTGAAGATTTCAGATACCATGATCCAGAAACGAACGAAAAATATATTCCATATTGTATCGAGCCATCACt        >  2:764590/1‑141 (MQ=255)                                                                                                                            tggttggGGTGAGTTATGGGGTATCGCAAGTCGTACAGACTTCGACTTACGTAAACATGCTGAACACTCTGGTGAAGATTTCAGATACCATGATCCAGAAACGAACGAAAAATATATTCCATATTGTATCGAACCATAACt        >  1:475027/1‑141 (MQ=255)                                                                                                                              gttggGGTGAGTTATGGGGTATCGCAAGTCGTACAGACTTCGACTTACGTAAACATGCTGAACACTCTGGTGAAGATTTCAGATACCATGATCCAGAAACGAACGAAAGATATATCTCATATTGTATTGAGCAATCACTTg      >  1:204985/1‑141 (MQ=255)                                                                                                                              gttggGGTGAGTTATGGGGTATCGCAAGTCGTACAGACTTCGACTTACGTAAACATGCTGAACACTCTGGTGAAGATTTCAGATACCATGATCCAGAAACGAACGAAAAATATATTCCATATTGTATCGAGCCATCACTTg      >  2:422154/1‑141 (MQ=255)                                                                                                                              gttggGGTGAGTTATGGGGTATCGCAAGTCGTACAGACTTCGACTTACGTAAACATGCTGAACACTCTGGTGAAGATTTCAGATACCATGATCCAGAAACGAACGAAAAATATATTCCATATTGTATCGAGCCATCACTTg      >  1:399642/1‑141 (MQ=255)                                                                                                                                tggGGTGAGTTATGGGGTATCGCAAGTCGTACAGACTTCGACTTACGTAAAAATGCTGAACACTCTGGTGAAGATTTCAGATACCATGATCCAGAAACGAACGAAAAATATATTCCATATTGTATCGAGCCATCACTTGGt    <  1:106289/141‑1 (MQ=255)                                                                                                                                 ggggTGAGTTATGGGGTATCGCAAGTCGTACAGACTTCGACTTACGTAAACATGCTGAACACTCTGGTGAAGATTTCAGATACCATGATCCAGAAACGAACGAAAAATATATTCCATATTGTATCGAGCCATCACTTGGTg   >  2:714712/1‑141 (MQ=255)                                                                                                                                 ggggTGAGTTATGGGGTATCGCAAGTCGTACAGACTTCGACTTACGTAAACATGCTGAACACTCTGGTGAAGATTTCAGATACCATGATCCAGAAACGAACGAAAAATATATTCCATATTGTATCGAGCCATCACTTGGTg   <  2:796693/141‑1 (MQ=255)                                                                                                                                 ggggTGAGTTATGGGGTATCGCAAGTCGTACAGACTTCGACTTACGTAAACATGCTGAACACTCTGGTGAAGATTTCAGATACCATGATCCAGAAACGAACGAAAAATATATTCCATATTGTATCGAGCCATCACTTGGTg   >  2:739483/1‑141 (MQ=255)                                                                                                                                 ggggTGAGTTATGGGGGATCGCAAGTCGTACAGACTTCGACTTACGTAAACATGCTGAACACTCTGGTGAAGATTTCAGATACCATGATCCAGAAACGAACGAAAAATATATTCCATCTTGTATCGAGCCATCACTTGGTg   >  2:274651/1‑141 (MQ=255)                                                                                                                                 ggggTGAGTTAGGGGGGATCGCAGGTCGTACAGACTTCGACTTACGTAAAGATGCTGAAGACTCTGGTGAAGATTTCAGATACCATGATCCAGAAACGAACGAAAAATATATTCCATATTGTATCGAGCCATCACTTGGTg   <  2:724382/141‑1 (MQ=255)                                                                                                                                  gggTGAGTTATGGGGTATCGCAAGTCGTACAGACTTCGACTTACGTAAACATGCTGAACACTCTGGTGAAGATTTCAGATACCATGATCCAGAAACGAACGAAAAATATATTCCATATTGTATCGAGCCATCACTTGGTGc  <  2:6855/141‑1 (MQ=255)                                                                                                                                       |                                                                                                                                        TGCAAGTGACTGGTTAACAAGCTTAAATATGAGCAGTGAAAATATGCGTTTACGTGATCATGATGAAGATGAATTATCTCATTACTCAAATGCAACAACTGATATTGAATATAAATTCCCATTTGGTTGGGGTGAGTTATGGGGTATCGCAAGTCGTACAGACTTCGACTTACGTAAACATGCTGAACACTCTGGTGAAGATTTCAGATACCATGATCCAGAAACGAACGAAAAATATATTCCATATTGTATCGAGCCATCACTTGGTGC  >  CP000730/1677597‑1677866

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑