Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,794,352 A→T 5.4% Y431N (TAT→AAT)  USA300HOU_1678 ← hypothetical membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,794,3520AT 5.4% 280.8 / 4.4 91Y431N (TAT→AAT) USA300HOU_1678hypothetical membrane protein
Reads supporting (aligned to +/- strand):  ref base A (38/47);  new base T (3/2);  total (42/49)
Fisher's exact test for biased strand distribution p-value = 6.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.20e-01

CTTGGTAATTTTTATTGATTGGTGACGGATGATGCATATAATGTTCAAACGGCAATGATTCCACTGTATAAACCTCTTGTCGTGGATGATCTTTAATTTGTTGTTTTAATAACTGAACTCTTTTTTCATGTTCATAATGAACATAAATAAATGCACTAAGATATATCACAGCTAAAACTAGTGATGCACCTTTAATAAAATTAACATTGATTGACTTATATTTCCGAAATTCTTTTAATAAAATAATTAAAATGATTACATGTATCGTATAAA  >  CP000730/1794217‑1794489
                                                                                                                                       |                                                                                                                                          
ctTGGTAATTTTTATTGATTGGTGACGGATGATGCATATAATGTTCAAACGGCAATGATTCCACTGTATAAACCTCTTGTCGTGGATGATCTTTAATTTGTTGTTTTAATAACTGAACTCTTTTTTCATGTTCATTATGaa                                                                                                                                       <  1:59313/141‑1 (MQ=255)
ctTGGTAATTTTTATTGATTGGTGACGGATGATGCATATAATGTTCAAACGGCAATGATTCCACTATATAAACCTCTTGTCGTGGATGATCTTTAATTTGTTGTTTTAATAACTGAACTCTTTTTTCATGTTCATTATGaa                                                                                                                                       <  2:239681/141‑1 (MQ=255)
 ttGGTAATTTTTATTGATTGGTGACGGATGATGCATATAATGTTCAAACGGCAATGATTCCACTGTATAAACCTCTTGTCGTGGATGATCTTTAATTTGTTGTTTTAATAACTGAACTCTTTTTTTTTGTTCTTAATGAAc                                                                                                                                      >  2:608007/1‑141 (MQ=255)
  tGGTAATTTTTATTTATTGGTGACGGATGATGCATATAATGTTCAAACGGCAATGATTCCACTGTATAAACCTCTTGTCGTGGATGATCTTTAATTTGTTGTTTTAATAACTGAACTCTTTTTTCATGTTCATAATGAACa                                                                                                                                     <  2:638611/141‑1 (MQ=255)
  tGGTAATTTTTATTGATTGGTGACGGATGTTTCATATAATGTTCAAACGGCAATGATTCCACTGTATAAACCTCTTGTCGTGGATGATCTTTAATTTGTTGTTTTAATAACTGAATTTTGTATTCATGTTCATAATGAACa                                                                                                                                     <  2:567261/141‑1 (MQ=255)
  tGGTAATTTTTATTGATTGGTGACGGATGATGCATATAATGTTCAAACGGCAATGATTCCACTGTATAAACCTCTTGTCGTGGATGATCTTTAATTTGTTGTTTTAATAACTGAACTCTTTTTTCATGTTCATAATGAACa                                                                                                                                     <  1:448887/141‑1 (MQ=255)
  tGGTAATTTTTATTGATTGGTGACGGATGATGCATATAATGTTCAAACGGCAATGATTCCACTGTATAAACCTCTTGTCGTGGATGATCTTTAATTTGTTGGTTTAATAACTGAACTCTTTTTTCATGTTCATAATGAACa                                                                                                                                     >  1:623924/1‑141 (MQ=255)
  tGGTAATTTTTATTGATTGGTGACGGATGATGCATATAATGTGCAAACGGCAATGATTCCACTGTATAAACCTCTTGTCGTGGATGATCTTTAATTTGTTGTTTTAATAACTGAACTCTTTTTTCATGTTCATAATGAACa                                                                                                                                     <  2:112404/141‑1 (MQ=255)
   ggTAATTTTTATTGATTGGTGACGGATGATGCATATAATGTTCAAACGGCAATGATTCCACTGTATAAACCTCTTGTCGTGGATGATCTTTAATTTGTTGTTTTAATAACTGAACTCGTTTTTAATGTTCATAATGAAAat                                                                                                                                    >  1:406604/1‑141 (MQ=255)
    gTAATTTTTATTGATTGGTGATGGATGATGCCTATAATGATCAAACGGCAATGATTCCACTGTATAAACCTCTTGTCGTGGATGATCTTTAATTTGTTGTTTTAATAACTGAACTCTTTTTTCATGTTCATAATGAACata                                                                                                                                   >  1:718287/1‑141 (MQ=255)
     tAATTTTTATTGATTGGTGACGGATGATGCATATAATGTTCAAACGGCAATGATTCCACTGTATAAACCTCTTGTCGTGGATGATCTTTAATTTGTTGTTTTAATAACTGAACTCTTTTTTCATGTTCATAATGAACataa                                                                                                                                  <  1:235101/141‑1 (MQ=255)
       aTTTTTATTGATTGGTGACGGATGATGCATATAATGTTCAAACGGCAATGATTCCACTGTATAAACCTCTTGTCGTGGATGATCTTTAATTTGTTGTTTTAATAACTGAACTCTTTTTTCATGTTCATAATGAACataaat                                                                                                                                <  1:784684/141‑1 (MQ=255)
       aTTTTTAGTGATTGGGGACGGAGGATGCATATAATGTTCAAACGGCAATGATTCCACTGTATAAACCTCTTGTCGTGGATGATCTTTAATTTGTTGTTTTAATAACTGAACTCTTTTTTCATGTTCATAATGAACataaat                                                                                                                                <  1:527609/141‑1 (MQ=255)
        tttttATTGATTGGTGACGGATGATGCATATAATGTTCAAACGGCAATGATTCCACTGTATAAACCTCTTGTCGTGGATGATCTTTAATTTGTTGTTTTAATAACTGAACTCTTTTTTCATGTTCATAATGAACataaata                                                                                                                               >  1:724034/1‑141 (MQ=255)
            tatcgctgggTGACGGATGATGCATATAATGTTCAACCGGCAATGATTCCACTGTATAAACCTCTTGTCGTGGATGATCTTTAATTTGTTGTTTTAATAACTGAACTCTTTTTTCATGTACATAGTGAACATAAATAAATg                                                                                                                           <  1:416520/133‑1 (MQ=255)
            tATTGATTGGTGACGGATGATGCATATAATGTTCAATCGGAAATGATTCCACTGTATAAACCTCTTGTCGTGGATGATCTTGAATTTGTTGTTTTAATAACTGAACTCTTTTTTCATGTTCATAATGAACATAAATAAATg                                                                                                                           <  2:358932/141‑1 (MQ=255)
            tATTGATTGGTGACGGATGATGCATATAATGTTCAAACGGCAATGATTCCACTGTATAAACCTCTTGTCGTGGATGATCTTTAATTTGTTGTTTTAATAACTGAACTCTTTTTTCATGTTCATAATGAACATAAATAAATg                                                                                                                           <  2:628285/141‑1 (MQ=255)
            tATTGATTGGTGACGGATGATGCATATAATGTTCAAACGGCAATGATTCCACTGTATAAACCTCTTGTCGTGGATGATCTTTAATTTGCTGTTTTAATAACTGAACTCTTTTTTCATGTTCATAATGAACATAAATAAATg                                                                                                                           <  1:469011/141‑1 (MQ=255)
            tATTGACTGTTGCCGCATGATGCATCCAAGTTTCACACGGCAATGATCCCCCTGATGAATCGGCGTTTCGTGGATGATCTTTAATTTGTTGTTTTAACAACTGAACTCTTTTTTCATGTTCATAATGAACATAAATAAATg                                                                                                                           <  1:186315/141‑1 (MQ=255)
             attgattgGTGACGGATGATGCATATAATGTTCAAACGGCAATGATTCCACTGTATAAACCTCTTGTCGTGGATGATCTTTAATTTGTTGTTTTAATAACTGAACTCTTTTTTCATGTTCATAATGAACATAAATAAATGc                                                                                                                          <  1:528089/141‑1 (MQ=255)
             attgattgGTCACGGATGATGCATATAATGTTAAATCGGCAATAATTCCACTGTATAAACCTCTTGTCGTGGATGATCTTTAATTTGTTGTTTTAATAACCGAACTCTTTTTATGTCTTCATAATGAACATAAATAAATGc                                                                                                                          <  1:193720/141‑1 (MQ=255)
              ttgattgGTTACGGATGATGCATATAATGTTCAAACGGCAATGATTCCACGGTATAAACCTCTTGTCGTGGATGATCTTTAATTTGTTGTTTTAATAACTGAACTCTTTTTTCATGTTCATAATGAACATAAATAAATGCa                                                                                                                         <  1:152258/141‑1 (MQ=255)
              ttgattgGTGACGGATGATGCATATAATGTTCAAACGGCAATGATTCCACTGTATAAACCTCTTGTCGTGGATGATCTTTAATTTGTTGTTTTGATAACTGAACTCTTTTTTCATGTTCATAATGAACATAGATAAATGCa                                                                                                                         <  2:519331/141‑1 (MQ=255)
              ttgattgGTGACGGATGATGCATATAATGTTCAAACGGCAATGATTCCACTGTATAAACCTCTTGTCGTGGATGATCTTTAATTTGTTGTTTTAATAACTGAACTCTTTTTTCATGTTCATAATGAACATAAATAAATGCa                                                                                                                         <  1:161537/141‑1 (MQ=255)
                tattgGTGACGGATGATGCATATAATGTTCAAACGGCAATGATTCCACTGTATAAACCTCTTGTCGTGGATGATCTTTAATTTGTTGTTTTAATAACTGAACTCTTTTTTCATGTTCATAATGAACATAAATAAATGCACt                                                                                                                       <  1:327905/140‑1 (MQ=255)
                ggtgggTGACGGATGATCGAGATAATGTTCAAACGGCAATGATTCCACTGTAGAAACCTCTTGTCGTGGATGATCTTTAAATTGTTGCTTTAATACCTGAACTCTTTTTTCATGTTCATAATGAACATAAATAAATGCACt                                                                                                                       <  1:299037/137‑1 (MQ=255)
                gattgGTGACGGATGATGCATATAATGTTCAAACGGCAATGATTCCACTGTATAAACCTCTTGTCGTGGATGATCTTTAATTTGTTGTTTTAATAACTGAACTCTTTTTTCATGTTCATAATGAACATAAATAAATGCACt                                                                                                                       >  1:88267/1‑141 (MQ=255)
                    ggTGACGGATGATGCATATAATGTTCAAACGGCAATGATTCCACTGTATAAACCTCTTGTCGTGGATGATCTTTAATTTGTTGTTTTAATAACTGAACTCTTTTTTCATGTTCATAATGAACATAAATAAATGCACTAAGa                                                                                                                  <  1:326994/141‑1 (MQ=255)
                      tGACGGATGATGCATATAATGTTCAAACGGCAATGATTCCACTGTATAAACCTCTTGTCGTGGATGATCTTTAATTTGTTGTTTTAATAACTGAACTCTTTTTTCATGTTCATAATGAACATAAATAAATGCACTAAGata                                                                                                                >  2:708245/1‑141 (MQ=255)
                         cGGATGATGCATATAATGTTCAAACGGCAATGATTCCACTGTATAAACCTCTTGTCGTGGATGATCTTTAATTTGTTGTTTTAATAACTGAACTCTTTTTTCATGTTCATAATGAACATAAATAAATGCACTAAGatatat                                                                                                             >  1:55633/1‑141 (MQ=255)
                             tgatgCATATAATGTTCAAACGGCAATGATTCCACTGTATAAACCTCTTGTCGTGGATGATCTTTAATTTGTTGTTTTAATAACTGAACTCTTTTTTCATGTTCATAATGAACATAAATAAATGCACTAAGATATATcaca                                                                                                         >  1:447538/1‑141 (MQ=255)
                               attcATATAATTTTCAAACGGCAATGATTCCACTGTATAAACCTCTTGTCGTGGATGATTTTTAATTTGTTGTTTTAATAACTGAACTCTTTTTTCATGTTCATAATGAATATAAATAAATGCACTAAGATATATCACAGc                                                                                                       <  2:206263/138‑1 (MQ=255)
                               atgCATATAATTTTCAAACGGCAATGATTCCACTGTATAAACCTCTTGTCGTGGATGATCTTTAATTTGTTGTTTTAATAACTGAACTCTTTTTTCATGTTCATAATGAACATAAATAAATGCACTAAGATATATCACAGc                                                                                                       <  2:511781/141‑1 (MQ=255)
                               atgCATATAATGTTCAAACGGCAATGATTCCACTGTATAAACCTCTTGTCGTGGATGATCTTTAATTTGTTGTTTTAATAACTGAACTCTTTTTTCATGTTCATAATGAACATAAATAAATGCACTAAGATATATCACAGc                                                                                                       <  1:255055/141‑1 (MQ=255)
                                         tGTTCAAACTGCAATGATTCCACTGTATAAACCTCTTGTCGTGGATGATCTTTAATTTGTTGTTTTAATAACTGAACTCTTTTTTCATGTTCATAATGAACATAAATAAATGACTCTAGATAGATCACAGCTAAAACTAGt                                                                                             <  2:779074/141‑1 (MQ=255)
                                         tGTTCAAACGGCAATGATTCCACTGTATAAACCTCTTGTCGTGGATGATCTTTAATTTGTTGTTTTAATAACTGAACTCTTTTTTCATGTTCATAATGAACATAAATGAATGCACTAAGATATATCACAGCTAAAACTAGt                                                                                             >  2:115588/1‑141 (MQ=255)
                                            ttaaaCGGGAATGATTCCACTGTATGACCCGCTTGTCGTGGATGATCTTTAATTTGTTGTTTTAATAACTGAACTCTTTTTTCATGTTCATAATGAACATAAATAAATGCACTAAGATATATCACAGCTAAAACTAGTGAt                                                                                          <  1:65704/139‑1 (MQ=255)
                                                  ggCAATGATTCCACTGTATAAACCTCTTGTCGTGGATGATCTTTAATTTGTTGTTTTAATAACTGAACTCTTTTTTCATGTTCATAATGAACATAAATAAATGCACTAAGATATATCACAGCTAAAACTAGTGATGCACCt                                                                                    <  1:666634/141‑1 (MQ=255)
                                                       tGATTCCACTGTATAAACCTCTTGTCGTGGATGATCTTTAATTTGTTGTTTTAGTAACTGAACTCTTTTTTCATGTTCATAATGAACATAAATAAATGCACTAAGATATATCACAGCTAAAACTAGTGATGCACCTTtaat                                                                               >  1:69899/1‑141 (MQ=255)
                                                       tGATTCCACTGTATAAACCTCTTGTCGTGGATGATCTTTAATTTGTTGTTTTAATAACTGAACTCTTTTTTCATGTTCATAATGAACATAAATAAATGCACTAAGATATATCACAGCTAAAACTAGTGATGCACCTTtaat                                                                               <  1:638343/141‑1 (MQ=255)
                                                          ttCCACTGTATAAAACTCTTGGCGTGGATGATCTTTAATTTGTTGTTTTAATAACTGAACTCTTTTTTCATGTTCATAATGAACATAAATAAATGCACTAAGATATATCACAGCTAAAACTAGTGATGCACCTTTAATaaa                                                                            >  1:803115/1‑141 (MQ=255)
                                                                  tataATCCTCTTGTCGTGTATGATCTTTAATTTGTTGTTTTAATAACTGAACTCTTTTTTCATGTTCATAATGAACATAAATAAATGCACTAAGATATATCACAGCTAAAACTAGTGCTGCACTTTTAAAAAAATTAACat                                                                    <  2:711080/141‑1 (MQ=255)
                                                                  tataAGCCTCTTGTCGTGGATGATCTTTAATTTGTTGTTTTAATAACTGAACTCTTTTTTCATGTTCATAATGAACATAAATAAATGCACTAAGATATATCACAGCTAAAACTAGTGATGCACCTTTAATAAAATTAACat                                                                    <  2:725358/141‑1 (MQ=255)
                                                                  tataAATCTCTTGTCGTGGATGATCTTTAATTTGTTGTTTTAATAACTGAACTCTTTTTTCATGTTCATAATGAACATAAATAAATGCACTAAGATATATCACAGCTAAAACTAGTGATGCACCTTTAATAAAATTAACat                                                                    <  2:21611/141‑1 (MQ=255)
                                                                  tataAACCTCTTGTCGTGGATGATCTTTAATTTGTTGTTTTAATAACTGAACTCTTTTTTCATGTTCATAATGAACATAAATAAATGCACTAAGATCTATCACAGCTAAAACTAGTGATGCGCCTTTAATAAAATTAACat                                                                    <  1:443999/141‑1 (MQ=255)
                                                                  tataAACCTCTTGTCGTGGATGATCTTTAATTTGTTGTTTTAATAACTGAACTCTTTTTTCATGTTCATAATGAACATAAATAAATGCACTAAGATATATCACAGCTAAAACTAGTGATGCACCTTTAATAAAATTAACat                                                                    <  1:323793/141‑1 (MQ=255)
                                                                   ataAACCTCTTGTCGTGGATGATCTTTAATTTGTTGTTTTAATAACTGAACTCTTTTTTCATGTTCATAATGAACATAAATAAATGCACTAAGATATATCACAGCTAAAACTAGTGATGCACCTTTAATAAAATTAACatt                                                                   >  2:749737/1‑141 (MQ=255)
                                                                   ataAACCTCTTGTCGCGGATGATCTTTACTTTGTTGTTTTAATAACTGAACTCTTTTTTCATGTTCATAATGAACATAAATAAATGCACTAAGATATATCACAGCTAAAACTAGTGATGCACCTTTCATATAATTAACatt                                                                   >  1:600518/1‑141 (MQ=255)
                                                                     aaaCCTCTTGTCGTGGATGATCTTTAATTTGTTGTTTTAATAACTGAACTCTTTTTTCATGTTCATAATGAACATAAATAAATGCACTAAGATATATCACAGCTAAAACTAGTGATGGACCTGTAAGAAAATTAACattga                                                                 <  1:801298/141‑1 (MQ=255)
                                                                     aaaCCTCTTGTCGTGGATGATCTTTAATTTGTTGTTTTAATAACTGAACTCTTTTTTCATGTTCATAATGAACATAAATAAATGCACTAAGATATATCACAGCTAAAACTAGTGATGCACCTTTAATAAAATTAACattga                                                                 >  2:465501/1‑141 (MQ=255)
                                                                               tCGTGGATGATCTTTAATTTGTTGTTTTAATAACTGAACTCTTTTTTCATGTTCATAATGAACATAAATACATACACGGATATATATCACAGCTAAAACTAGTGATGCACCTTTAATAAAATTAACATTGATTGACTtata                                                       >  1:793914/1‑141 (MQ=255)
                                                                               tCGTGGATGATCTTTAATTTGTTGTTTTAATAACTGAACTCTTTTTTCATGTTCATAATGAACATAAATAAATGCACTAAGATATATCACAGCTAAAACTAGTGATGCACCTTTAATAAAATTAACATTGATTGACTtatt                                                       >  1:684066/1‑140 (MQ=255)
                                                                               tCGCGGATGAGCTTTAATTTGTTGGTTTAATAACTGAACTCTTTTTTCATGTTCATCATTAACATAAATAAATGCACTAAGATATATCACAACTAAAAATGGGGATGCACCTTTAATAAAATTAACATTAATTGACTtata                                                       >  2:619861/1‑141 (MQ=255)
                                                                                  tGGATGATCTTTAATTTGTTGTTTTAATAACTGAACTCTTTTTTCATGTTCATAATGAACATAAATAAATGCACTAAGATATATCACAGCTAAAACTAGTGATGCACCTTTAATAAAATTAACATTGATTGACTTATAttt                                                    >  1:445634/1‑141 (MQ=255)
                                                                                   ggATGATCTTTAATTTGTTGTTTTAATAACTGAACTCTTTTTTTTTTTTTTTTCTGAACATAAATAACTGCACTAAGATATATCCCAGCTTAAAATAGTGATGCAGCTTTATTCAAATTTTCTTTTTTTGACTGATttttt                                                   >  2:74600/1‑140 (MQ=255)
                                                                                   ggATGATCTTTAATTTGTTGTTTTAATAACTGAACTCTTTTTTCATGTTCATAATGAACATAAATAAATGCACTAAGATATATCACAGCTAAAACTAGTGATGCACCTTTAATAAAATTAACATTGATTGACTTATATTTc                                                   <  2:220901/141‑1 (MQ=255)
                                                                                         tCTTTAATTTGTTGTTTTAATAACTGAACTCTTTTTTCATGTTCATAATGAACATAAATAAATGCACTAAGATATATCACAGCTAAAACTAGTGATGCACCTTTAATAAAATTAACATTGATTGACTTATATTTCCGAAAt                                             >  2:746144/1‑141 (MQ=255)
                                                                                          ttttAATTTGTTGTTTTAATAACTGAACTCTTTTTTCATGTTCATAATGAACATAAATAAATGCACTAAGATATATCACAGCTAAAACTAGTGATGCACCTTTAATAAAATTAACATTGATTGACTTATATTTCCGAAAtt                                            <  2:515669/140‑1 (MQ=255)
                                                                                          ttttAATTTGTTGTTTTAATAACTGAACTCTTTTTTCATGTTCATAATGAACATAAATAAATGCACTAAGATATATCACAGCTAAAACTAGTGATGCACCTTTAATAAAATTAACATTGATTGACTTATATTTCCGAAAtt                                            <  2:773938/140‑1 (MQ=255)
                                                                                          cTTTAATTTGTTGTTTTAATAACTGAACTCTTTTTTCATGTTTATAATGAAAATAAATAAATGCACTAAGATATATCACAGCTAAAACTAGTGATGCACCTTTAATAAAATTAACATTGATTAACTTATATTTACGAAAtt                                            >  2:694252/1‑141 (MQ=255)
                                                                                          cTTTAATTTGTTGTTTTAATAACTGAACTCTTTTTTCATGTTCATAATGAACATAAATAAATGCACTAAGATATATCACAGCTAAAACTAGTGATGCACCTTTAATAAAATTAACATTGATTGACTTATATTTCCGAAAtt                                            >  2:777549/1‑141 (MQ=255)
                                                                                               aTTTGTTGTTTTAATAACTGAACTCTTTTTTCATGTTCATAATGAACATAAATAAATGCACTAAGATATATCACAGCTAAAACTAGTGATGCACCTTTAATAAAATTAACATTGATTGACTTATATTTCCGAAATTCtttt                                       >  2:485679/1‑141 (MQ=255)
                                                                                                  tgttgtTTTAATAACTGAACTCTTTTTTCATGTTCATAATGAACATAAATAAATGCACTAGGATATATCACAGCTAAAACTAGTGATGCACCTTTAATAAATTTAACATTGATTGCCTTATATTTCCGACATTCTTTtaca                                    >  1:753869/1‑139 (MQ=255)
                                                                                                    ttgttTTAATAACTGAACTCTTTTTTCATGTTCATAATGAACATAAATAAATGCACTAAGATATATCACAGCTAAAACTAGTGATGCACCTTTAATAAAATTAACATTGATTGACTTATATTTCCAACCTTTTTTtaataa                                  >  1:413218/1‑141 (MQ=255)
                                                                                                     tgttTTAATAACTGAACTCTTTTTTTTTTTTTTTTATGAACATAAATAAATGCACTAAGATATATCACAGCTAAAACTAGTGAGGCACCTTTAATAAAATTAACATTGTTTTACTTATATTTCCGAAATTCTTTTattaaa                                 >  2:793013/1‑141 (MQ=255)
                                                                                                     tgttTTAATAACTGAACTCTTTTTTTTTTTTTATAATGAACATAAATAAATGCACTAAGATATATCACAGCTAAAACTAGTGATGCACCTTTAATAAAATTAACATTGATTGACTTATATTTCCGAAATTTTTTtattttt                                 >  2:757532/1‑136 (MQ=255)
                                                                                                     tgttTTAATAACTGAACTCTTTTTTCATGTTCATAATGAACATAAATAAATGCACTAAGATATATCACAGCTAAAACTAGTGATGCACCTTTAATAAAATTAACATTGATTGACTTATATTTCCGAAATTCTTTTaataaa                                 >  2:248581/1‑141 (MQ=255)
                                                                                                               aCTGAACTCTTTTTTCATGTTCATAATGAACATAAATAAATGCACTAAGATATATCACAGCTAAAACTAGTGATGCACCTTTAATAAAATTAACATTGATTGACTTATATTTCCGAAATTCTTTTAATAAAATAATTaaaa                       >  1:327197/1‑141 (MQ=255)
                                                                                                               aCTGAACTCTTTTTTCATGTTCATAATGAACATAAATAAATGCACTAAGATATATCACAGCTAAAACTAGTGATGCACCTTTAATAAAATTAACATTGATTGACTTATATTTCCGAAATTCTTTTAATAAAATAATTaaaa                       >  1:373102/1‑141 (MQ=255)
                                                                                                               aCTGAACTCTTTTTTCATGTTCATAATGAACATAAATAAATGCACTAAGATATATCACAGCTAAAACTAGTGATGCACCTTTAATAAAATTAACATTGATTGACTTATATTTCCGAAATTCTTTTAATAAAATAATTaaaa                       >  2:713821/1‑141 (MQ=255)
                                                                                                               aCTGAACTCTTTTTTCATGTTCATAATGAACATAAATAAATGCACTAAGATATATCACAGCTAAAACTAGTGATGCACCTTTAATAAAATTAACATTGATTGACTTATATTTCCGAAATTCTTTTAATAAAATAATTaaaa                       <  1:449626/141‑1 (MQ=255)
                                                                                                                cTGAACTCTTTTTTCATGTTCATAATGAACATAAATAAATGCACTAAGAAATATCACAGCTAAAATTAGTGATGCAACTTTTATAAAATTAACATTGAATGAATTATCATTCCCCAATTCATTTAAAAAAAAAAATaacaa                      >  2:52495/1‑138 (MQ=255)
                                                                                                                  gAACTCTTTTTTCATGTTCATAATGAACATAAATAAATGCACTAAGATATATCACAGCTAAAACTAGTGATGCACCTTTAATAAAATTAACATTGATTGACTTATATTTCCGAAATTCTTTTAATAAAATAATTAAAATGa                    >  1:92202/1‑141 (MQ=255)
                                                                                                                    aCTCTTTTTTCATGTTCATAATGAACATGAATAAATGCACTAAGATATATCACAGCTAAAACTAGTGATGCACCTTTAATAAAATTAACATTGATTGACTTATATTTCCGAAATTTTTTTATTAATATGATTAAAATGAtt                  >  1:675672/1‑141 (MQ=255)
                                                                                                                    aCTCTTTTTTCATGTTCATAATGAACATAAATAAATGCACTAAGATATATCACAGCTAAAACTAGTGATGCACCTTTAATAAAATTAACATTGATTGACTTATATTTCTGAGATTCGTTTAATAACAACATTAAAATGAtt                  >  2:638538/1‑141 (MQ=255)
                                                                                                                    aCTCTTTTATCATGTTCATAATGAACATAAATAAATGAACTAAGATATATCACATCTAAAACTAGTGATGCACCTTTAATAAAATTAACATTCATGAACTTATCTTTCCGAAATCTTTTTAATAAAATTATTTAaataata                  >  2:141258/1‑140 (MQ=255)
                                                                                                                     ttctTTTTTCATGTTCATAATGAACATAAATAAATGCACTAAGATATATCACAGCTAAAACTAGTGATGCACCTTTAATAATTTTAATATTGATTGACTTATATTTCCGAAATTCTTTTAATAAAATAATTAAAATGATTa                 <  2:489294/140‑1 (MQ=255)
                                                                                                                     ctctTTTTTCATGTTCATAATGAACATAAATAAATGCACTAAGATATATCACAGCTAAAACTAGTGATGCACCTTTAATAAAATTAACATTGATTGACTTATATTTCCGAAATTCTTTTAATAAAATAATTAAAATGATTa                 <  1:708245/141‑1 (MQ=255)
                                                                                                                     ctctTTTTTCATGTTCATAATGAACATAAATAAATGCACTAAGATATATCACAGCTAAAACTAGTGATGCACCTTTAATAAAATTAACATTGATTGACTTATATTTCCGAAATTCTTTTAACAAAATAATTAAAATGATTa                 <  1:485679/141‑1 (MQ=255)
                                                                                                                     ctattttttATTGTTCATAATGAACATAAAATAATGCACTAAGATTTATCACAGCTAAAACTAGTGATGCACCTTTAAGAATATAAACATTTATTGACTTATATTTCCGCAATTCTTTTAATAAAATAATTAAAATGATTa                 <  2:623796/138‑1 (MQ=255)
                                                                                                                          ttttCATGTTTATAATGAACATAAATAAATGCACTAAGATATATCACAGCTAAAACTAGTGATGCACCTTTAATAAAATTAACATTGATTGACTTATATTTCCGAAATTCTTTTAATAAAATAATTAAAATGATTACATGt            <  2:17760/141‑1 (MQ=255)
                                                                                                                           tttCATGTTCATAATGAACATAAATAAATGCACTAAGATATATCACAGCTAAAACTAGTGATGCACCTTTAATAAAATTAACATTGATTGACTTATATTTCCGAAATTCTTTTAATAAAATAATTAAAATGATTACATGTa           <  2:88267/141‑1 (MQ=255)
                                                                                                                              cATGTTCATAATGAACATAAATAAATGCACTAAGATATATCACAGCTAAAACTAGTGATGCACCTTTAATAAAATTAACATTGATTGACTTATATTTCCGAAATTCTTTTAATAAAATAATTAAAATGATTACATGTATCg        <  1:644214/141‑1 (MQ=255)
                                                                                                                              cATGTTCATAATGAACATAAATAAATGCACTAAGATATATCACAGCTAAAACTAGTGATGCACCTTTAATAAAATTAACATTGATTGACTTATATTTCCGAAATTCTTTTAATAAAATAATTAAAATGATTACATGTATCg        <  2:803115/141‑1 (MQ=255)
                                                                                                                              cATGTTCATAATGAACATAAATAAATGCACTAAGATATATCACAGCTAAAACTAGTGATGCACCTTTAATAAAATTAACATTGATTGACTTATATTTCCGAAATTCTTTTAATAAAATAATTAAAATGATTACATGTATCg        >  1:703317/1‑141 (MQ=255)
                                                                                                                               aTGTTCATTATGAACATGAATAAATGCACTAAGATATATCACAGCTAAAACTAGTGATGCCCCTTTAATAAAATTAACATTGATTGACTTATATTTCCGAAATTCTTTTAATAAAATTATTAAAATGATTACATGCATCGt       >  2:469144/1‑141 (MQ=255)
                                                                                                                                 gTTCATAATGAACATAAATAAATGCACTAAGATATATCACCGCTAAAAGTAGTGATGCACCTTTAATACAATTAACATTGATTGACTTATATTTCCGGAATTCTTTTAAGAAAATAATGAAATTCATTACATCTTTAGtat     >  1:643150/1‑141 (MQ=255)
                                                                                                                                  ttCATAATGAACATAAATAAATGCACTAAGATATATCACAGCTAAAACTAGTGATGCACCTTTAATAAAATTAACATTGATTGACTTATATTTCCGAAATTCTTTTAATAAAATAATTAAAATGATTACATGTATCGtata    <  1:220004/141‑1 (MQ=255)
                                                                                                                                    cATAATGAACATAAATAAATGCACTAAGATATATCACAGCTAAAACTAGTGATGCACCTTTAATAAAATTAACATTGATTGACTTATATTTCCGAAATTCTTTTAATAAAATAATTAAAATGATTACATGTATCGTATaaa  >  2:575988/1‑141 (MQ=255)
                                                                                                                                    cATAATGAACATAAATAAATGCACTAAGATATATCACAGCTAAAACTAGTGATGCACCTTTAATAAAATTAACATTGATTGACTTATATTTCCGAAATTCTTTTAATAAAATAATTAAAATGATTACATGTATCGTATaaa  >  2:277889/1‑141 (MQ=255)
                                                                                                                                    cATAATGAACATAAACAAATTTACTAAGATATATCACATCTATAACCAGTGATGCAACTGTAATAAAATTAACGTTGATTGACTTATATTGTCGAAATTCTTTTAATAAAATAATTAAAATGATTACATGTATCGTATaaa  <  2:65689/141‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                          
CTTGGTAATTTTTATTGATTGGTGACGGATGATGCATATAATGTTCAAACGGCAATGATTCCACTGTATAAACCTCTTGTCGTGGATGATCTTTAATTTGTTGTTTTAATAACTGAACTCTTTTTTCATGTTCATAATGAACATAAATAAATGCACTAAGATATATCACAGCTAAAACTAGTGATGCACCTTTAATAAAATTAACATTGATTGACTTATATTTCCGAAATTCTTTTAATAAAATAATTAAAATGATTACATGTATCGTATAAA  >  CP000730/1794217‑1794489

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: