Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	1,874,676	T→C	5.4%	E178G (GAA→GGA)	rsuA ←	pseudouridylate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,874,676	0	T	C	5.4%	220.5 / 3.6	75	E178G (GAA→GGA)	rsuA	pseudouridylate synthase
Reads supporting (aligned to +/- strand):  ref base T (31/39);  new base C (2/2);  total (34/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.86e-01

ATCATTTTCAGTTAATAAACGATATTCTCCTGAATCTAAATTGCTGTCCAATTCTAAATCAGCAATTTTGATACGTCTTAAATGTAATACCTCATTTTGAATGCTATGAAACATTCGTTTAACTTGATGATATTTTCCTTCATAAATTGTTACGTGTGACGTTTGATTATCAATATAAGTTAATATTGCAGGCTTAACCTTGCCATCAGTCAGTGTTACACCCTCTTTAAAAGCTTGAATGTCGTCTTCAGTGATAGGATTTGCTGAAATAACTTCATA  >  CP000730/1874537‑1874815                                                                                                                                            |                                                                                                                                            aTCATTTTCAGTTAATAAACGATATTCTCCTGAATCTAAATTGCTGTCCAATTCTAAATCAGCAATTTTGATACGTCTTAAATGTAATACCTCATTTTGAATGCTATGAAACATTCGTTTAACTTGATGATATTTTCCTTc                                                                                                                                            <  2:699896/141‑1 (MQ=255)  tCATTTTCAGTTAATAAACGATATTCTCCTGAATCTAAATTGCTGTCCAATTCTAAATCAGCAATTTTGATACGTCTTAAATGTAATACCTCATTTTGAATGCTATGAAACATTCGTTTAACTTGATGATATTTTCCTTCa                                                                                                                                           >  2:7291/1‑141 (MQ=255)   cATTTTCAGTTAATAAACGATATTCTCCTGAATCTAAATTGCTGTCCAATTCTAAATCAGCAATTTTGATACGTCTTAAATGTAATACCTCATTTTGAATGCTATGAAACATTCGTTTAACTTGATGATATTTTCCTTCAt                                                                                                                                          <  2:735876/141‑1 (MQ=255)             taataaACGATATTCTCCTGAATCTAAATTGCTGTCCAATTCTAAATCAGCAATTTTGATACGTCTTAAATGTAATACCTCATTTTGAATGCTATGAAACATTCGTTTAACTTGATGATATTTTCCTTCATAAATTGTTAc                                                                                                                                <  1:692447/141‑1 (MQ=255)             taataaACGATATTCTCCTGAATCTAAATTGCTGTCCAATTCTAAATCAGCAATTTTGATACGTCTTAAATGTAATACCTCATTTTGAATGCTATGAAACATTCGTTTAACTTGATGATATTTTCCTTCATAAATTGTTAc                                                                                                                                <  1:433775/141‑1 (MQ=255)              aataaACGATATTCTCCTGAATCTAAATTGCTGTCAAATTCACAATCAGCAATTTTGATACGTCTTAAATGTAATACCTCATTTTGAATGCTATGAAACATTCGTTTAACTTGATGATATTTTACTTCATAAATCGTTCCg                                                                                                                               >  2:133090/1‑141 (MQ=255)               ataaACCATATTCTCCTCAATCTAAATTGCTGTCCAATTCTAAATCAGCAATTTTGATACGTCTTAAATGTAATACCTCATTTTGAATGCTATGAAACATTCGTTTAACTTGATGATATTTTCCTTCATAAATTGTTACgt                                                                                                                              <  2:160988/141‑1 (MQ=255)                taaACGATATTCTCCTGAATCTAAATTGCTGTCCAATTCTAAATCAGCAATTTTGATACGTCTTAAATGTAATACCTCATTTTGAATGCTATGAAACATTCGTTTAACTTGATGATATTTTCCTTCATAAATTGTTACgtg                                                                                                                             <  1:481998/141‑1 (MQ=255)                taaACGATATTCTCCTGAATCTAAATTGCTGTCCAATTCTAAATCAGCAATTTTGATACGTCTTAAATGTAATACCTCATTTTGAATGCTATGAAACATTCGTTTAACTTGATGATATTTTCCTTCATAAATTGTTACgtg                                                                                                                             <  2:494001/141‑1 (MQ=255)                 aaaCGATATTCTCCTGAATCTAAATTGCTGTCCAATTCTAAATCAGCAATTTTGATACGTCTTAAATGTAATACCTCATTTTGAATGCTATGAAACATTCGTTTAACTTGATGATATTTTCCTTCATAAATTGTTACgtgt                                                                                                                            <  1:585522/141‑1 (MQ=255)                 aaaCGATATTCTCCTGAATCTAAATTGCTGTCCAATTCTAAATCAGCAATTTTGATACGTCTTAAATGTAATACCTCATTTTGAATGCTATGAAACATTCGTTTAACTTGATGATATTTTCCTTCATAAATTGTTACgtgt                                                                                                                            >  1:27204/1‑141 (MQ=255)                  aaCGATATTCTCCTGAATCTAAATTGCTGTCCAATTCTAAATCAGCAATTTTGATACGTCTTAAATGTAATACCTCATTTTGAATGCTATGAAACATTCGTTTAACTTGATGATATTTTCCTTCATAAATTGTTACGtgtg                                                                                                                           >  1:523260/1‑141 (MQ=255)                     gATATTCTCCTGAATCTAAATTGCTGTCCAATTCTAAATCAGCAATTTTGATACGTCTTAAATGTAATACCTCATTTTGAATGCTATGAAACATTCGTTTAACTTGATGATATTTTCCTTCATAAATTGTTACGTGTGACg                                                                                                                        <  1:98543/141‑1 (MQ=255)                        atTCTCCTGAATCTAAATTGCTGTCCAATTCTAAATCAGCAATTTTGATACGTCTTAAATGTAATACCTCATTTTGAATGCTATGAAACATTCGTTTAACTTGATGATATTTTCCTTCATAAATTGTTACGTGTGACGttt                                                                                                                     <  2:600252/141‑1 (MQ=255)                        atTCTCCTGAATCTAAATTGCTGTCCAATTCTAAATCAGCAATTTTGATACGTCTTAAATGTAATACCTCATTTTGAATGCTATGAAACATTCGTTTAACTTGATGATATTTTCCTGCATAACTTGTTACGTGTGACGttt                                                                                                                     >  2:352005/1‑141 (MQ=255)                        atTCTCCTGAATCTAAATTGCTGTCCAATTCGAAATCAGCAATTTTGATACGTCTTAAATGTAATACCTCATTTTGAATGCTATGAAACATTCGTTTAACTTGATGATATTTTCCTTCATAAATTGTTACGTGTGACGttt                                                                                                                     <  1:443330/141‑1 (MQ=255)                        atTCCCCTGAATCTAAATTGCTGTCCAATTCTAAATCAGCAATTTTGATACGTCTTAAATGTAATACCTCATTTTGAATGCTATGAAACATTCGTTTAACTTGATGATATTTTCCTTCATAAATTGTTACGTGTGACGttt                                                                                                                     <  1:472664/141‑1 (MQ=255)                          tctcCTGTATCTAAATTGCTGTCCAATTCTAAATCAGCAATTTTGATACGTCTTAAATGTAATACCTCATGTTGACTGCTAGGAAACATGCGCTTATCTTGATGATATTTTCCTTCATAAATTGTTACGTGTGACGTTTGa                                                                                                                   <  2:46423/141‑1 (MQ=255)                           ctcCTGAATCTAAATTGCTGTCCAATTCTAAATCAGCAATTTTGATACGTCTTAAATGTAATACCTCATTTTGAATGCTATGAAACATTCGTTTAACTTGATGATATTTTCCTTCATAAATTGTTACGTGTGACGTTTGAt                                                                                                                  >  2:583795/1‑141 (MQ=255)                             ccTGAATCTAAATTGCTGTCCAATTCTAAATCAGCAATTTTGATACGTCTTAAATGTAATACCTCATTTTGAATGCTATGAAACATTCGTTTAACTTGATGATATTTTCCTTCATAAATTTTTACGTGTGACGTTTGATTa                                                                                                                >  1:727291/1‑141 (MQ=255)                             ccTGAATCTAAATTGCTGTCCAATTCTAAATCAGCAATTTTGATACGTCCTAAATGTAATACCTCATTTTGAATGCTATGAAACATTCGTTTAACTTGATGATATTTTCCTTCATAAATTGTTACGTGTGACGTTTGATTa                                                                                                                >  1:513040/1‑141 (MQ=255)                                 aaTCTAAATTGCTGTCCAATTCTAAATCAGCAATTTTGATACGTCTTAAATGTAATACCTCATTTTGAATGCTATGAAACATTCGTTTAACTTGATCATATTTTCCTTCATAAATTGTTACGTGTGACGTTTGATTATCaa                                                                                                            <  1:459959/141‑1 (MQ=255)                                   tCTATATTGCTTTCCAATTCTACATCAGCATTTTTTATACGTCTTCAATGTAATACCTCATTTTGAATGCTATGAAACATTCGTTTAACTTGATGATATTTTCCTTCATAAATTGTTACGTGTGACGTTTGATTATCAata                                                                                                          <  1:168001/141‑1 (MQ=255)                                   tCTAAATTGCTGTCGAATTCTAAATCAGCAATTTTGATACATCTTAAATGTTATACCCCATTTTGAATGCTATGATACATTCGTTTAACTTGATGATATTTTCCTTCATAAATTGTTACTTGTGACGTTTGATTATCAata                                                                                                          <  2:219630/141‑1 (MQ=255)                                   acTAAATTGCTGTCCAATTCTAAATCAGCAATTTTGATACGTCTTAAATGTAATACCTCATTTTGAATGCTATGAAACATTCGTTTAACTTGATGATATTTTCCTTCATAAATTGTTACGTGTGACGTTTGATTATCAata                                                                                                          <  2:419582/140‑1 (MQ=255)                                       aagtGCTGTCCAATTCTAAATCAGCAATTTTGATACGTCTAAAATGTAATACCTCATTTTGAATGCTATGAAACATTCGTTTAACTTGATGATATTTTCATTCATAAACTATTACGTGTGACCTTTGATTATCacattaca                                                                                                      >  2:338352/4‑134 (MQ=255)                                       aaTTGCTGTCCAATTCTAAATCAGCAATTTTGATACGTCTTAAATGTAATACCTCATTTTGAATGCTATGAAACATTCGTTTAACTTGATGATATTTTCCTTCATAAATTGTTACGTGTGACGTTTGATTATCAATTTAAg                                                                                                      <  1:550307/141‑1 (MQ=255)                                       aaTTGCTGTCCAATTCTAAATCAGCAATTTTGATACGTCTTAAATGTAATACCTCATTTTGAATGCTATGAAACATTCGTTTAACTTGATGATATTTTCCTTCATAAATTGTTACGTGTGACGTTTGATTATCAATATAAg                                                                                                      >  2:13424/1‑141 (MQ=255)                                       aaTTGCTGTCCAATTCTAAATCAGCAATTTTGATACGTCTTAAATGTAATACATCATTTTGAATGCTATGAAACATTCGTTTAACTTGATGATATTTTCCTTCATAAATCGTTACGTGTGACGTTTGATTCTCAATATAAg                                                                                                      >  2:82358/1‑141 (MQ=255)                                            cTGTCCAATTCTAAATCAGCAATTTTGATACGTCTTAAATGTAATACCTCATTTTGAATGCTATGAAACATTCGTCTAACTTGGTGTTAGCTTCCTTCATATCTTGTTGCTTGTGACGTTTGATTATCAATATAAGTTAat                                                                                                 <  2:170746/141‑1 (MQ=255)                                            cTGTCCAATTCTAAATCAGCAATTTTGATACGTCTTAAATGTAATACCTCATTTTGAATGCTATGAAACATTCGTCTAACTTGATGATATTTTCCTTCATAAATTGTTACGTGTGACGTTTGATTATCAATATAAGTTAat                                                                                                 <  2:734103/141‑1 (MQ=255)                                                  aactcaaaaTCAGCAATTTTCATACTTCTTAAATGTAATACCTCACTTTGAATGCTATGAAACATTCGTTTAACTTGATGATATTTTCCTTCATAAATTGTTACGTGTGACGTTTGATTATCAATATAAGTTAATATTGCa                                                                                           <  2:108622/135‑1 (MQ=255)                                                  aaTTCTAAATCAGCAATTTTGATACGTCTTAAATGTAATATCTCATTTTGAATGCTATGAAACATTCGTTTAACTTGATGATATTTTCCTTCATAAATTGTTACGTGTGACGTTTGATTATCAATATAAGTTAATATTGCa                                                                                           <  2:412249/141‑1 (MQ=255)                                                  aaTTCCAAATCAGCAATTTTGATACGTCTTAAACGTAATACCTCATTTTGAATGCTATGAAACATTCGTTTAACTTGATCATATTTTCCTTCATAAATTGTTACGTGTGACGTTTGATTATCAATATAAGTTAATATTGCa                                                                                           <  2:667120/141‑1 (MQ=255)                                                      cTAAATCAGCAATTTTGATACGTCTTAAATGTAATACCTCATTTTGAATGCTATGAAACATTCGTTTAACTTGATGATATTTTCCTCCATAAATTGTTACGTGTGACGTTTGATTATCAATATAAGTTAATATTGCAGGCt                                                                                       >  2:107078/1‑141 (MQ=255)                                                      cTAAATCAGCAATTTTGATACGTCTTAAATGTAATACCTCATTTTGAATGCTATGAAACATTCGTTTAACTTGATGATATTTTCCTCCATAAATTGTTACGTGTGACGTTTGATTATCAATATAAGTTAATATTGCAGGCt                                                                                       >  2:664256/1‑141 (MQ=255)                                                           tCAGCAATTTTGATACGTCTTAAATGTAATACCTCATTTTGAATGCTATGAAACATTCGTTTAACTTGATGATATTTTCCTTCATAAATTGTTACGTGTGACGTTTGATTATCAATATAAGTTAATATTGCAGGCTTAAcc                                                                                  >  1:362615/1‑141 (MQ=255)                                                             aGCAATTTTGATACGTCTTAAATGTAATACCTCATTTTGAATGCTATGAAACATTCGTTTAACTTGATGATATTTTCCTTCATAAATTGTTACGTGTGACGTTTGATTATCAATATAAGTTAATATTGCAGGCTTAACCtt                                                                                <  1:404415/141‑1 (MQ=255)                                                               taaTTTTGATACGTCTCAAATGTAATACCTCATTTTGAATGCTATGAAACATTCGTTTAACTTGATGATATTTTCCTTCATAAATTGTTACGTGTGACGTTTGATTATCAATATAAGTTAATATTGCAGGCTTAACCTTGc                                                                              <  2:703856/140‑1 (MQ=255)                                                               aaaTTTTGATACGTCTTAAATGTAATACCTCATTTTGAATGCTATGAAACATTCGTTTAACTTGATGATATTTTCCTTCATAAATTGTTACGTGTGACGTTTGATTATCAATATAAGTTAATATTGCAGGCTTAACCTTGc                                                                              <  2:52366/140‑1 (MQ=255)                                                                   tttGATACGTCTTAAATGTAATAACTCGTTTTGAATGCTATGAAACATTCGTTTAACTCGATGATATTTTCCTTCATAAATTGTTACGTGTGACGTTTGATTAGCAATATACGTTAATATTGCAGGCTTAACCTTGCCAtc                                                                          <  2:603910/141‑1 (MQ=255)                                                                   tttGATACATCTTAAATGTATAACCGCTGGGTGAATGCTATGATACATTCGTTTAACTTGATGATATTTTCCTTCATAAATTGTTACGTGTGACGTTTGATTATCAATATAAGTTAATATTGCAGGATTAACCTTGCCAtc                                                                          <  2:603985/141‑1 (MQ=255)                                                                         aCGTTTTAAATGTAATACCTCATTTTGAATGCTATGAAACATTCGTTTAACTTGATGATATTTTCCTTCATAAATTGTTACGTGTGACGTTTGATTATCAATATAAGTTAATATTGCAGGCTTAACCTTGCCAtcagtcag                                                                    <  2:63793/141‑1 (MQ=255)                                                                             ctcaaaTGTAATACCTCATTTTGAATGCTATGAAACATTCGTTTAACTTGATGATATTTTCCTTCATAAATTGTTACGTGTGACGTTTGATTATCAATATAAGTTAATATTGCAGGCTTAACCTTGCCATCAGTCAGTGtt                                                                <  2:94766/138‑1 (MQ=255)                                                                                   tGTAATACCGTATTTTGAATGCTATGAAACATTCGTTTAACTTGATGATATTTTCCTTCATAAATTGTTACGTGTGACGTTTGATTATCAATATAAGTTAATATTGCAGGCTTAACCTTGCCATCAGTCAGTGTTACAccc                                                          <  1:10452/141‑1 (MQ=255)                                                                                     tAATACCTCATTTTGAATGCTATGAAACATTCGTTTAACTTGATGATATTTTCCTTCATAAATTGTTACGTGTGACGTTTGATTATCAATATAAGTTAATATTGCAGGCTTAACCTTGCCATCAGTCAGTGTTACACCctc                                                        >  2:405614/1‑141 (MQ=255)                                                                                     tAATACCTCATTTTGAATGCTATGAAACATTCGTTTAACTTGATGATATTTTCCTTCATAAATTGTTACGTGTGACGTTTGATTATCAATATAAGTTAATATTGCAGGCTTAACCTTGCCATCAGTCAGTGTTACACCctc                                                        >  2:250065/1‑141 (MQ=255)                                                                                     tAATACCTCATTTTGAATGCTATGAAACATTCGTTTAACTTGATGATATTTTCCTTCATAAATTGTTACGTGTGACGTTTGATTATCAATATAAGTTAATATTGCAGGCTTAACCTTGCCATCAGTCACTGTTACACCctc                                                        >  2:263386/1‑141 (MQ=255)                                                                                       aTACCTCATTTTGAATGCTATGAAACATTCGTTTAACTTGATGATATTTTCCTTCATAAATTGTTACGTGTGACGTTTGATTATCAATATAAGTTAATATTGCAGGCTTAACCTTGCCATCAGTCAGTGTTACACCCTCtt                                                      >  2:132636/1‑141 (MQ=255)                                                                                        tACCTCATTTTGAATGCTATGAAACATTCGTTTAACTTGATGATATTTTCCTCCATAAATTGTTACGTGTGACGTTTGATTATCAATATAAGTTAATATTGCAGGCTTAACCTTGCCATCAGTCAGTGTTACACCCTCttt                                                     <  1:664256/141‑1 (MQ=255)                                                                                        tACCTCATTTTGAATGCTATGAAACATTCGTTTAACTTGATGATATTTTCCTCCATAAATTGTTACGTGTGACGTTTGATTATCAATATAAGTTAATATTGCAGGCTTAACCTTGCCATCAGTCAGTGTTACACCCTCttt                                                     <  1:107078/141‑1 (MQ=255)                                                                                         aCCTCATTTTGAATGCTATGAAACATTCGTTTAACTTGATGATATTTTCCTTCATAAATTGTTACGTGTGACGTTTGATTATCAATATAAGTTAATATTGCAGGCTTAACCTTGCCATCAGTCAGTGTTACACCCTCTTTa                                                    >  2:704034/1‑141 (MQ=255)                                                                                          ccTCATTTTGAATGCTATGAAACATTCGTTTAACTTGATGATATTTTCCTTCATAAATTGTTACGTGTGACGTTTGATTATCAATATAAGTTAATATTGCAGGCTTAACCTTGCCATCAGTCAGTGTTCCACCCTCTTTaa                                                   >  2:245336/1‑141 (MQ=255)                                                                                          ccTCATTTTGAATGCTATGAAACATTCGTTTAACTTGATGATATTTTCCTTCATAAATTGTTACGTGTGACGTTTGATTATCAATATAAGTTAATATTGCAGGCTTAACCTTGCCATCAGTCAGTGTTACACCCTCTTTaa                                                   <  1:7291/141‑1 (MQ=255)                                                                                          ccTCATTTTGAATGCTATGAAACATTCGTTTAACTTGATGATATTTTCCTTCATAAATTGTTACGTGTGACGTTTGATTATCAATATAAGTTAATATTGCAGGCTTAACCTTGCCATCAGTCAGTGTTACACCCTCTTTaa                                                   >  2:767660/1‑141 (MQ=255)                                                                                          ccTCATTTTGAATGCTATGAAACATTCGTTTAACTTGATGATATTTTCCTTCATAAATTGTTACGTGTGACGTTTGATTATCAATATAAGTTAATATTGCAGGCTTAACCTTGCCATCAGTCAGTGTTACACCCTCTTTaa                                                   >  2:207562/1‑141 (MQ=255)                                                                                             cATTTTGAATGCTATGAAACATTCGTTTAACTTGATGATATTTTCCTTCATAAATTGTTACGTGTGACGTTTGATTATCAATATAAGTTAATATTGCAGGCTTAACCTTGCCATCAGTCAGTGTTACACCCTCTTTaaaaa                                                >  2:682410/1‑140 (MQ=255)                                                                                             cATTTTGAATGCTATGAAACATTCGTTTAACTTGATGATATTTTCCTTCATAAATTGTTACGTGTGACGTTTGATTATCAATATAAGTTAATATTGCAAGCTTAACCTTGCCCTCAGTCAGTGTTACACCCTCTTTAAAAg                                                >  1:238348/1‑141 (MQ=255)                                                                                                  tGAATGCTATGAAACATTCGTTTAACTTGATGATATTTTCCTTCATAAATTGTTACGTGTGACGTTTGATTATCAATATAAGTTAATATTGCAGGCTTAACCTTGCCATCAGTCAGTGTTACACCCTCTTTAAAAGCTTGa                                           <  2:236969/141‑1 (MQ=255)                                                                                                    aaTGCTATGAAACATTCGTTTAACTTGATGATATTTTCCTTCATAATTTGTTACGTGTGACGTTTGATTATCAATATAAGTTAATATTGCAGGCTTAACCTTGCCATCAGTCAGTGTTACACCCTCTTTAAAAGCTTGAAt                                         >  1:427953/1‑141 (MQ=255)                                                                                                    aaTGCTATGAAACATTCGTTTAACTTGATGATATTTTCCTTCATAAATTGTTACGTGTGACGTTTGATTATCAATATAAGTTAATATTGCAGGCTTAACCTTGCCATCAGTCAGTGTTACACCCTCTTTAAAAGCTTGAAt                                         >  1:499255/1‑141 (MQ=255)                                                                                                     aTGCTATGAAACATTCGTTTAACTTGATGATATTTTCCTTCATAAATTGTTACGTGTGACGTTTGATTATCAATATAAGTTAATATTGCAGGCTTAACCTTGCCATCAGTCAGTGTTACACCCTCTTTAAAAGCTTGAATg                                        >  2:546552/1‑141 (MQ=255)                                                                                                     aTGCTATGAAACATTCGTTTAACTTGATGATATTTTCCTTCATAAATTGTTACGTGTGACGTTTGATTATCAATATAAGTTAATATTGCAGGCTTAACCTTGCCATCAGTCAGTGTTACACCCTCTTTAAAAGCTTGAATg                                        >  2:731140/1‑141 (MQ=255)                                                                                                        cTATGAAACATTCGTTTAACTTGATGATATTTTCCTTCATAAATTGTTACGTGTGACGTTTGATTATCAATATAAGTTAATATTGCAGGCTTAACCTTGCCATCAGTCAGTGTTACACCCTCTTTAAAAGCTTGAATgtcg                                     >  2:197646/1‑141 (MQ=255)                                                                                                        cTATGAAACATTCGTTTAACTTGATGATATTTTCCTTCATAAATTGTTACGTGTGACGTTTGATTATCAATATAAGTTAATATTGCAGGCTTAACCTTGCCATCAGTCAGTGTTACACCCTCTTTAAAAGCTTGAATgtcg                                     <  1:690927/141‑1 (MQ=255)                                                                                                        cTATGAAACATTCGTTTAACTTGATGATATTTTCCTTCATAAATTGTTACGTGTCGCGTTTGATTATCAATATAAGTTAATATTGCAGGCTTAACCTTGCCATCAGTCAGTGTTACACCCTCTTTAAAAGCTTGAATgtcg                                     <  1:424297/141‑1 (MQ=255)                                                                                                         tATGAAACGTTCGTTTAACTTGATGATATTTTCCTTCATAAATTGTTACGTGTGACGTTTGATTATCAATATAAGTTAATATTGCAGGCTTAACCTTGCCATCATTCAGTGTTACACCCTCTTTAAAAGCTTGAATgtcgt                                    <  2:513040/141‑1 (MQ=255)                                                                                                         tATGAAACATTCGTTTAACTTGATTATATTTTCCTTCATAAATTGTTACGTGTGACGTTTGATTATCAATATAAGTTAATATTGCAGGCTTAACCTTGCCATCAGTCAGTGTTACACCCTCTTTAAAAGCTTGAATgtcgt                                    <  2:727291/141‑1 (MQ=255)                                                                                                          aTGAAACATTCGTTTAACTTGATGATATTTTCCTTCATAAATTGTTACGTGTGACGTTTGATTATCAATATAAGTTAATATTGCAGGCTTAACCTTGCCATCAGTCAGTGTTACACCCTCTTTAAAAGCTTGAATgtcgtc                                   <  2:34273/141‑1 (MQ=255)                                                                                                                    cGTTTAACTTGATGATATTTTCCTTCATAAATTGTTACGTGTGACGTTTGATTATCAATATAAGTTAATATTGCAGGCTTAACCTTGCCATCAGTCAGTGTTACACCCTCTTTAAAAGCTTGAATGTCGTCTTCAGTGATa                         >  1:189295/1‑141 (MQ=255)                                                                                                                       ttAACTTGATGATATTTTCCTTCATAAATTGTTACGTGTGACGTTTGATTATCAATATAAGTTAATATTGCAGGCTTAACCTTGCCATCAGTCAGTGTTACACCCTCTTTAAAAGCTTGAATGTCGTCTTCAGTGATAGGa                      >  2:98317/1‑141 (MQ=255)                                                                                                                       ttAACTTGATGATATTTTCCTTCATAAATTGTTACGTGTGACGTTTGATTATCAATATAAGTTAATATTGCAGGCTTAACCTTGCCATCAGTCAGTGTTACACCCTCTTTAAAAGCTTGAATGTCGTCTTCAGTGATAGGa                      >  2:776329/1‑141 (MQ=255)                                                                                                                       ttAACTTGATGATATTTTCCTTCATAAATTGTTACGTGTGACGTTTGATTATCAATATAAGTTAATATTGCAGGCTTAACCTTGCCATCAGTCAGTGTTACACCCTCTTTAAAAGCTTGAATGTCGTCTTCAGTGATAGGa                      >  1:416286/1‑141 (MQ=255)                                                                                                                           cTTGATGATATTTTCCTTCATAAATTGTTACGTGTGACGTTTGATTATCAATATAAGTTAATATTGCAGGCTTAACCTTGCCATCAGTCAGTGTTACACCCTCTTTAAAAGCTTGAATGTCGTCTTCAGTGATAGGATTTg                  <  2:603395/141‑1 (MQ=255)                                                                                                                            ttGATGATATTTTCCTTCATAAATTGTTACGTGTGACGTTTGATTATCAATATAAGTTAATATTGCAGGCTTAACCTTGACATCAGTCAGTGTTACACCCTCTTTAAAAGCTGGAATGTCGTCTTCAGTGATAGGATTTGc                 >  1:65206/1‑141 (MQ=255)                                                                                                                                           ttCTTAAATTGTGACGTGTGACGTTTGATTATTAATATAAGTTAATATTGCAGGCCTAACCTTGCCATCAGTGAGTGTTACACCCTCTTTAATAGCTTGAATGTCGTCTTCAGTGATAGGATTTGCTGATATAACTTCatt  <  2:432421/141‑2 (MQ=255)                                                                                                                                            |                                                                                                                                            ATCATTTTCAGTTAATAAACGATATTCTCCTGAATCTAAATTGCTGTCCAATTCTAAATCAGCAATTTTGATACGTCTTAAATGTAATACCTCATTTTGAATGCTATGAAACATTCGTTTAACTTGATGATATTTTCCTTCATAAATTGTTACGTGTGACGTTTGATTATCAATATAAGTTAATATTGCAGGCTTAACCTTGCCATCAGTCAGTGTTACACCCTCTTTAAAAGCTTGAATGTCGTCTTCAGTGATAGGATTTGCTGAAATAACTTCATA  >  CP000730/1874537‑1874815

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑