Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,186,126 G→T 7.1% S12* (TCA→TAA)  fliA ← DNA‑directed RNA polymerase sigma subunit FliA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,186,1260GT 7.1% 267.9 / 5.9 95S12* (TCA→TAA) fliADNA‑directed RNA polymerase sigma subunit FliA
Reads supporting (aligned to +/- strand):  ref base G (42/44);  new base T (5/3);  total (47/48)
Fisher's exact test for biased strand distribution p-value = 7.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01

ATGTGATTGTCCTTTAGAATATTTATATGCCAATGACTCAATTAGTTTTTGGTAATGTTTAACTAACTTATCCTGTGCATCTGTATTCTTATTTTCTTGGTGTTCTTTAATCCATTGGTTAATTTGCTCAGGTGAAATTTCATTAGCTGATTTCGACTCTTTCGCCATTATTTCGCACCTGCTCTTTTTTTATATACTTAGTCATACTGATTGTCACACCAGATTCTTTATATACTGTGACTTCATCCATTAAAGATTCGATTAAAAATAAA  >  CP000730/2185993‑2186264
                                                                                                                                     |                                                                                                                                          
atgtgatTGTCCTTTAGAATATTTATATGCCAATGACTCAATTAGTTTTTGGTACTGTTTAACTAACTTATCCTGTGCATCTGTATTCTTATTTTCTTGGTGTTCTCTAATCCATTGGTCAATTTGCTCAGGTTAAAtttg                                                                                                                                     >  2:364984/1‑140 (MQ=255)
atgtgatTGTCCTTTAGAATATTTATATGCCAATGACTCAATTAGTTTTTGGTAATGTTTAACTAACTTATCCTGTGCATCTGTATTCTTATTTTCTTGGTGTTCTTTAATCCATTGGTTAATTTGCTCAGGTGAAATTTc                                                                                                                                     >  1:784921/1‑141 (MQ=255)
   tgatTGTCCTTTAGAATATTTCTCTGAAGAAAGCTCAATTAGTTTTTGGTAATGTTTAACTAACTTATCCTGTGCATCTGTATTCTTATTTTCTTGGTGTTCTTTAATCCATTGGTTAATTTGCTCAGGTGAAATTTCAtt                                                                                                                                  >  1:799923/1‑141 (MQ=255)
   tgatTGTCCTTTAGAATATTTATATGTCAATGACTCAATTAGTTTTTGGTAATGTTTAACTAACTTATCCTGTGCATCTGTATTCTTATTTTCTTGGTGTTCTTTAATCCATTGGTTAATTTGCTCAGGTGAAATTTCAtt                                                                                                                                  <  2:707269/141‑1 (MQ=255)
   tgatTGTCCTTTAGAATATTTATATGCCAATGACTCAATTAGTTTTTTGTAATGTTTAACTAACTTATCCTGTGCATCTGTATTCTTATTTTCTTGGTGTTCTTTATTCCATTGTTTAATTTGCTCAGGTGAAATTTCAtt                                                                                                                                  <  2:336592/141‑1 (MQ=255)
   tgatTGTCCTTTAGAATATTTATATGCCAATGACTCAATTAGTTTTTGGTAATGTTTAACTAACTTATCCTGTGCATCTGTATTCTTATTTTCTTGGTGTTCTTTAATCCATTGGTTAATTTGCTCAGGTGAAATTTCAtt                                                                                                                                  >  1:768680/1‑141 (MQ=255)
    gatTGTCCTTTAGAATATTTATATGCCAATGACTCAATTAGTTTTTGGTAATGTTTAACTAACTTATCCTGTGCATCTGTATTCTTATTTTCTTGGTGTTCTTTAATCCATTGGTTAATTTGCTCAGGTGAAATTTCATTa                                                                                                                                 <  1:562182/141‑1 (MQ=255)
    gatTGTCCTTTAGAATATTTATATGCCAATGACTCAATTAGTTTTTGGTAATGTTTAACTAACTTATCCTGTGCATCTGTATTCTTATTTTCTTGGTGTTCTTTAATCCATTGGTTAATTTGCTCAGGTGAAATTTCATTa                                                                                                                                 <  1:124538/141‑1 (MQ=255)
      atGTCCTTTAGAAAATTTATATGCCAATGACTCAATTAGTTTTTGGTAATGTTTAAATAACTTATACCGTGCATCTGTATTCTTATTTTCTTAGTGTACTTTAATCAATTGATTAATTTGCTCAGGTGAAATTTAATTAGc                                                                                                                               >  2:340154/2‑141 (MQ=255)
        gTCCTTTAGAATATTTATATACCAATGACTCAATTAGTTTTTGGTAATGTTTAACTAACTTATCCTGTGCATCTGTATTCTTATTTTCTTGGTGTTCTTTAATCCATTGGTTAATTTGCTCAGGTGAAATTTCATTAGCTg                                                                                                                             >  1:492708/1‑141 (MQ=255)
          ccTCTAGGATATTTATGTGCAAATGACTCAATTAGTTTTTTGTCAAATTGAACTACCTTATCCTGTGCATCTGTATCCTTATTTTCTTGGTTTTCTTGACTCCATTTGTTAATTTGCTCATGTGAAATTTCATGAGCTGAt                                                                                                                           <  2:281161/141‑1 (MQ=255)
           cTTTAGAATATTTATATGCCAATGACTCAATTAGTTTTTGGTAATGTTTAACTAACTTATCCTGTGCATCTGTATTCTTATTTTCTTGGTGTTCTTTAATCCATTGGTTAATTTGCTCAGGTGAAATTTCATTAGCTGCtt                                                                                                                          >  2:351060/1‑141 (MQ=255)
           cTTTAGAATATTTATATGCCAATGACTCAATTAGTTTTTGGTAATGTTTAACTAACTTATCCTGTGCATCTGTATTCTTATTTTCTTGGTGTTCTTTAATCCATTGGTTAATTTGCTCAGGTGAAATTTCATTAGCTGAtt                                                                                                                          >  1:380323/1‑141 (MQ=255)
             ttAGAATATTTATATGCCAATGACTCAATTAGTTTTTGGTAATGTTTAACTAACTTATCCGGTGCATCTGTATTCTTATTTTCTTGGTGTTCTTTATTCCATTGGTTAATTTGCTGAGGTGCAATTTCATTAGCTGATTTc                                                                                                                        <  1:445416/141‑1 (MQ=255)
               aGAATATTTATATGCCAATGACTCAATTAGTTTTTGGTAATGTTTAACTAACTTATCCTGTGCATCTGTATTCTTATTTTCTTGGTGTTCTTTAATCCATTGGTTAATTTGCTCAGGTGAAATTTCATTCGCTGATTTctc                                                                                                                      >  2:68916/1‑139 (MQ=255)
                  atatTTATATGACAATGACTCAATTAGTTTTTGGTAATGTTTAACTAACTTATCCTGTGCATCTGTATTCTTATTTTCTTGGTGTTCTTTAATCCATTGGTTAATTTGCTCAGGTGAAATTTCATTAGCTGATTTCGActc                                                                                                                   <  1:682034/141‑1 (MQ=255)
                     tttATATGCCAATGACTCAATTAGTTTTTGGTAATGTTTAACTAACTTATCCTGTGCATCTGTATTCTTATTTTCTTGGTGTTCTTTAATCCATTGGTTAATTTGCTCAGGTGAAATTTCATTAGCTGATTTCGACTCttt                                                                                                                <  2:475242/141‑1 (MQ=255)
                       tataTGCCAATGACTCAATTAGTTTTTGGTAATGTTTAACTAACTTATCCTGTGCATCTGTATTCTTATTTTCTTGGTGTTCTTTAATCCATTGGTTAATTTGCTCAGGTGAAATTTCATTAGCTGATTTCGACTCTTTCg                                                                                                              <  1:501187/141‑1 (MQ=255)
                        atatGCCAATGACTCTATTTGTTTTTGGTCATGTTTAACTACCTTATCCTGTGCATCTGTATTCTTATTTTCTTGGTGTTCTTTAATCCATTGGTTAATTTGCTCATGTGAAATTTCATTAGCTGATTTCGACTCTTTCGc                                                                                                             <  2:243733/141‑1 (MQ=255)
                         tatGCCAATGACTCAATTAGTTTTTGGTAATGTTTAACTAACTTATCCTGTGCATCTGTATTCTTATTTTCTTGGTGTTCTTTAATCCATTGGTTAATTTGCTCAGGTGAAATTTCATTAGCTGATTTCGACTGTTTCGcc                                                                                                            >  1:412944/1‑141 (MQ=255)
                         tatGCCAATGACTCAATTAGTTTTTGGTAATGTTTAACTAACTTATCCTGTGCATCTGTATTCTTATTTTCTTGGTGTTCTTTAATCCATTGGTTAATTTGCTCAGGTGAAATTTCATTAGCTGATTTCGACTCTTTCGcc                                                                                                            <  2:660592/141‑1 (MQ=255)
                         tatGCCAATGACTCAATTAGTTTTTGGTAATGTTTAACTAACTTATCCTGTGCATCTGTATTCTTATTTTCTTGGTGTTCTTTAATCCATTGGTTAATTTGCTCAGGTGAAATTTCATTAGCTGATTTCGACTCTTTCGcc                                                                                                            <  2:509539/141‑1 (MQ=255)
                         tatGCCAATGACTCAATTAGTTTTTGGTAATGTTTAACTAACTTATCCTGTGCATCTGTATTCTTATTTTCTTGGTGTTCTTTAATCCATTGGTTAATTTGCTCAGGTGAAATTTCATTAGCTGATTTCGACTCTTTCGcc                                                                                                            <  1:658311/141‑1 (MQ=255)
                         tatGCCAATGACTCAATTAGTTTTTGGTAATGTTTAACTAACTTATCCTGTGCATCTGTATTCTTATTTTCTTGGTGTTCTTTAATCCATTGGTTAATTTGCTCAGGTGAAATTTCATTAGCTGATTTCGACTCTTTCGcc                                                                                                            <  2:546900/141‑1 (MQ=255)
                         tatGCCAATGACTCAATTAGTTTTTGGTAATGTTTAACTAACTTATCCTGTGCAACTGTATTCTTATTTTCTTGGGGTTCTTTATTATCGTTTTAAATTTGCTCAGGTTAAATTTCATTAGCTTCTTTCGGTTCTTTCGcc                                                                                                            >  1:352018/1‑141 (MQ=255)
                            gCCAATGACTCAATTAGTTTTTGGTAATGTTTAACTAACTTATCCTGTGCATCTGTATTCTTATTTTCTTGGTGTTCTTTAATCCATTGGTTAATTTGCTCAGGTGAAATTTCATTAGCTGATTTCGACTCTTTCGCCatt                                                                                                         <  1:495906/141‑1 (MQ=255)
                                 tGACTCAATTAGTTTTTGGTAATGTTTTACTAACTTATCCTGTGCATCTGTATTCTTATTTTCTTGGTGTTCTTTAATCCATTGGTTAATTTGATCAGGTGAAATTTCATTAGCTGATTTCGACTCTTTCGCCATTATTTc                                                                                                    <  2:523821/141‑1 (MQ=255)
                                     tCAATTAGTTTTTTTTTTTTTTTTACTAACTTATCCTGTGCATCTGTATTCTTATTTTCTTGGTGTTCTTTAATCCATTGGTTAATTTGCTCAGGTGAAATTTCATTAGCTGTTTTCGACTCTTTCGCCATTATTTCGCAc                                                                                                >  2:617820/1‑141 (MQ=255)
                                     tCAATTAGTTTTTGGTAATGTTTAACTAACTTATCCTGTGCATCTGTATTCTTATTTTCTTGGTGTTCTTTAATCCATTGGTTAATTTGCTCAGGTGAAATTTCATTAGCTGATTTCGACTCTTTCGCCATTATTTCGCAc                                                                                                >  2:611100/1‑141 (MQ=255)
                                     tCAATTAGTTTTTGGTAATGTTTAACTAACTTATCCTGTGCATCTGTATTCTTATTTTCTTGGTGTTCTTTAATCCATTGGTTAATTTGCTCAGGTGAAATTTCATTAGCTGATTTCGACTCTTTCGCCATTATTTCGCAc                                                                                                >  2:140187/1‑141 (MQ=255)
                                       aaTTAGTTTTTGGTAATGTTTAACTAACTTATCCTGTGCATCTGTATTCTTATTTTCTTGGTGTTCTTTAATCCATTGGTTAATTTGCTCAGGTGAAATTTCATTAGCTGATTTCGACTCTTTCGCCATTATTTCGCACCt                                                                                              <  2:621258/141‑1 (MQ=255)
                                        ctttgtctgtgcttaTGTTTAACTAACTTATCCTGTCCATCTGTATTCTTATTTTTTTGGTGTTCTTTAATCCATTGGTTAATTTGCTCAGGTGAACTTTCATTAGCTGATTTCGAGTCTTTCGCCATTATTTCGCACCTg                                                                                             <  2:283695/127‑1 (MQ=255)
                                          tAGTTTTTGGTTATGTTTAACTAACTTATCCTGTGCATCTGTATTCTTATTTTCTTGGTGTTCTTTAATCCATTGGTTAATTTGCTCAGGTGAAATTTCATTAGCTGATTTCGACTCTTTCGCCATTATTTCGCACCTGct                                                                                           <  2:522177/141‑1 (MQ=255)
                                          tAGTTTTTGGTAATGTTTAACTAACTTATCCTGTGAATCTGTAGTCTTATTTTCTTGGTGTTCTTTAATCCATTGGTTAATTTGCTCAGGTGAAATTTCATTAGCTGATTTCGACTCTTTCGCCATTATTTCGCACCTGct                                                                                           <  1:547831/141‑1 (MQ=255)
                                              ttttGGAAATGTTTAACTAACTTATCCTGTGCATCTGTATTCTTATTTTCTTGGTGTTCTTTAATCCATTGGTTAATTTGCTCAGGTGCAATTTCATTAGCTGATTTCGACTCTTTCGCCATTATTTCGCACCTGCTCttt                                                                                       >  2:508977/1‑141 (MQ=255)
                                                ttGGTAATGTTTAACTAACTTATCCTGTGCATCTGTATTCTTATTTTCTTGGTGTTCTTTAATCCATTGGTTAATTTGCTCAGGTGAAATTTCATTAGCTGATTTCGACTCTTTCGCCATTATTTCGCACCTGCTCttttt                                                                                     >  1:462603/1‑141 (MQ=255)
                                                ttGGTAATGTTTAACTAACTTATCCTGTGCATCTGTATTCTTATTTTCTTGGTGTTCTTTAATCCATTGGTTAATTTGCTCAGGTGAAATTTCATTAGCTGATTTCGACTCTTTCGCCATTATTTCGCACCTGCTCttttt                                                                                     >  1:669997/1‑141 (MQ=255)
                                                ttGGTAATGTTTAACTAACTTATCCTGTGCATCTGTATTCTTATTTTCTTGGTGTTCTTTAATCCATTGGTTAATTTGCTCAGGTGAAATTTCATTAGCTGATTTCGACTCTTTCGCCATTATTTCGCACCTAATCttttt                                                                                     >  2:199503/1‑141 (MQ=255)
                                                     aaTGTTTAACTAACTTATCCTGTGCATCTGTATTCTTATTTTCTTGGTGTTCTTTAATCCATTGGTTAATTTGCTCAGGTGAAATTTCATTAGCTGATTTCGTCTCTTTCGCCATTATTTCGCACCTGCTCTTTGTTtcta                                                                                >  2:354142/1‑141 (MQ=255)
                                                        gTTTAACTAGCTTATCCTGTGCATCTGTATTCTTATTTTCTTGGTGTTCTTTAATCCATTGGTTAATTTGCTCAGGTTAAATTTCATTAGCTGATTTCGACTCTTTCGCCATTATTTCGCACCTGCTCTTTTTTTATATAc                                                                             >  1:593104/1‑141 (MQ=255)
                                                        gTTTAACTAACTTATCCTGTGCATCTTTATTCTTATTTTCTTGGTGTTCTTTAATCCATTGGTTAATTTGCTCAGGTGAAATTTCATTAGCTGATTTCGACTCTTTCGCCATTATTTCGCACCTGCTCTTTTTTTATATAc                                                                             <  2:211799/141‑1 (MQ=255)
                                                        gTTTAACTAACTTATCCTGTGCATCTGTATTCTTATTTTCTTGGTGTTCTTTAATCCATTGGTTAATTTGCTCAGGTTAAATTTCATTAGCTGATTTCGACTCTTTCGCCATTATTTCGCACCTGCTCTTTTTTTATATAc                                                                             >  1:780385/1‑141 (MQ=255)
                                                             actatctTATCCTGTGCATCTGTATTCTTATTTTCTTGGTGTTCTTTAATCCATTGGTTAATTTGCTCAGGTGAAATTTCATTAGCTGATTTCGACTCTTTCGCCATTATTTCGCACCTGCTCTTTTTTTATATACTTAGt                                                                        <  2:645691/141‑1 (MQ=255)
                                                             actaactTATCCTGTGCATCTGTATTCTTATTTTCTTGGTGTTCTTTAATCCATTGGTTAATTTGCTCAGGTGAAATTTCATTAGCTGATTTCGACTCTTTCGCCATTATTTCGCACCTGCTCTTTTTTTATATACTTAGt                                                                        >  1:712727/1‑141 (MQ=255)
                                                             actaactTATCCTGTGCATCTGTATTCTTATTTTCTTGGTGTTCTTTAATCCATTGGTTAATTTGCTCAGGTGAAATTTCATTAGCTGATTTCGACTCTTTCGCCATTATTTCGCACCTGCTCTTTTTTTATATACTTAGt                                                                        >  1:334076/1‑141 (MQ=255)
                                                                aactTATCCTGTGCATCTGTATTCTTATTTTCTTGGTGTTCTTTAATCCATTGGTTAATTTGCTCAGGTGAAATTTCCTTAGCTGATTTCGACTCTTTCGCCATTATTTCGCACCTGCACTTTTTTTATATGCTTAGTCAt                                                                     >  2:114993/1‑141 (MQ=255)
                                                                aactTATCCTGTGCATCTGTATTCTTATTTTCTTGGTGTTCTTTAATCCATTGGTTAATTTGCTCAGGTGAAATTTCATTAGCTGATTTCGACTCTTTCGCCATTATTTCGCACCTGCTCTTTTTTTATATACTTAGTCAt                                                                     <  1:284386/141‑1 (MQ=255)
                                                                     ttCCTGTGCATCTGTATTCTTATTTTCTTGGTGTTCTTTAATCCATTGGTTAATTTGCTCAGGTGAAATTTCATTAGCTGATTTCGACTCTTTCGCCATTATTTCGCACCTGCTCTTTTTTTATATACTTAGTCATACTGa                                                                <  2:278883/140‑1 (MQ=255)
                                                                     aTCCTGTTCATCTGTATTCTTATTTTCTTGGTGTTCTTTAATCCATTGGTTAATTTGCTCAGGTGAAATTTCATTAGCTGATTTCGATTCTTTCGCCATTATTTCGCACCTGCTCTTTTTTTATATACTTAGTCATACTGa                                                                <  2:291738/141‑1 (MQ=255)
                                                                     aTCCTGTGCATCTGTATTCTTATTTTCTTGGTGTTCTTTAATCCATTGGTTAATTTGCTCAGGTGAAATTTCATTAGCTGATTTCGACTCTTTCGCCATTATTTCGCACCTGCTCTTTTTTTATATACTTAGTCATACTGa                                                                <  1:391491/141‑1 (MQ=255)
                                                                       ccTGTGCATTTGTTTTCTTATTTTCTTTGTGTTCTTTAATCCATTGGTTAATTTGCTCAGGTGAAATTTCATTAGCTGATTTCGACTCTTTCGCCATTATTTTGCACCTGCTCTTTTTTTATATACTTAGTCATACTGAtt                                                              <  2:787000/141‑1 (MQ=255)
                                                                       ccTGTGCATCTGTATTCTTATTTTCTTGGTGTTCTTTAATCCATTGGTTAATTTGCTCAGGTGAAATTTCATTAGCTGATTTCGACTCTTTCGCCATTATTTCGCACCTGCTCTTTTTTTATATACTTAGTCATACTGAtt                                                              <  1:601737/141‑1 (MQ=255)
                                                                       ccTGTGCATCTGTATTCTTATTTTCTTGGTGTTCTTTAATCCATTGGTTAATTTGCTCAGGTGAAATTTCATTAGCTGATTTCGACTCTTTCGCCATTATTTCGCACCTGCTCTTTTTTTATATACTTAGTCATACTGAtt                                                              <  1:88148/141‑1 (MQ=255)
                                                                           tgCATCTGTATTCTCATTTTCTTGGTGTTCTGTAATCCATTGGTTAATTTGATCAGGTGAAATATCATTAGCAGATTTCGCCTCTTTCGCCATTATTTCGAAACTGCTCTTTTTTTATATCTTTTGTCATACTTATTgcac                                                          >  2:102899/1‑138 (MQ=255)
                                                                            ttattcttATTCTTATTTTCTTTGTCTTCATTAAGCCTTTGGTTAATTTGCTCAGGTCAAATTTCATTAGCTGATTTCGACGCTTTTGCCATTATTTCGCACCTGCTCTTTTTTTATATACTTAGCCATACTGATTGCcac                                                         <  2:232029/134‑1 (MQ=255)
                                                                            tcATCTGTATTCTTATTTTCTTGTTGTTCTTTAATCCATTGGTTAATTTGCTCAGGTGAAATTTCATTAGCTGATTTCGACTCTTTCGCCATTATTTCGCACCTGCTCTTTTTTTATATACTTAGTCATACTGATTGTcac                                                         <  2:809038/140‑1 (MQ=255)
                                                                            gCATCTGTATTCTTATTTTCTTGGTGTTCTTTAATCCATTGGTTAATTTGCTCAGGTGAAATTTCATTAGCTGATTTCGACTCTTTCGCCATTATTTCGCACCTGCTCTTTTTTTATATACTTAGTCATACTGATTGTcac                                                         >  1:532428/1‑141 (MQ=255)
                                                                            gCATCTGTATTCTTATTTTCTTGGTGTTCTTTAATCCATTGGTTAATTTGCTCAGGTGAAATTTCATTAGCTGATTTCGACTCTTTCGCCATTATTTCGCACCTGCTCTTTTTTTATATACTTAGTCATACTGATTGTcac                                                         >  1:720998/1‑141 (MQ=255)
                                                                             cATCTGTATTCTTATTTTCTTGGTGTTCTTTAATCCATTGGTTAATTTGCTCAGGTGAAATTTCATTAGCTGATTTCGACTCTTTCGCCATTATTTCGCACCTGCTCTTTTTTTATATACTTAGTCATACTGATTGTcaca                                                        <  2:682029/141‑1 (MQ=255)
                                                                              aTCTGTATTCTTATTTTCTTGGTGTTCTTTAATCCATTGGTTAATTTGCTCAGGTTAAATTTCATTAGCTGATTTCGACTCTTTCGCCATTATTTCGCACCTGCTCTTTTTTTATATACTTAGTCATACTGATTGTCacac                                                       <  2:780385/141‑1 (MQ=255)
                                                                              aTCTGTATTCTTATTTTCTTGGTGTTCTTTAATCCATTGGTTAATTTGCTCAGGTTAAATTTCATTAGCTGATTTCGACTCTTTCGCCATTATTTCGCACCTGCTCTTTTTTTATATACTTAGTCATACTGATTGTCacac                                                       <  2:593104/141‑1 (MQ=255)
                                                                                cTGTATTCTTATTTTCTTGGTGTTCTTTAATCCATTGGTTAATTTGCTCAGGTGAAATTTCATTAGCTGATTTCGACTCTTTCGCCATTATTTCGCACCTGCTCTTTTTTTATATACTTAGTCATACTGATTGTCACACCa                                                     <  1:508977/141‑1 (MQ=255)
                                                                                 tGTATTCTTATTTTCTTGGTGTTCTTTAATCCATTGGTTAATTTGCTCAGGTGAAATTTCATTAGCTGATTTCGACTCTTTCGCCATTATTTCGCACCTGCTCTTTTTTTTTATATTTTTTCATCCTGATTGTCACGCCAg                                                    >  2:603690/1‑141 (MQ=255)
                                                                                 tGTATTCTTATTTTCTTGGTGTTCTTTAATCCATTGGTTAATTTGCTCAGGTGAAATTTCATTAGCTGATTTCGACTCTTTCGCCATTATTTCGCACCTGCTCTTTTTTTATCTTCTTTGTTATACTGATTGttaaactag                                                    >  1:210749/1‑133 (MQ=255)
                                                                                 tGTATTCTTATTTTCTTGGTGTTCGGTAATCCAGGGGTTAATTTGCTCAGTTGGAATTTCATTAGCTGATTTCGACTCTTTCGCCATTATTTCGCACCTGCTCTTTTTTTATATACTTAGTCATACTGATTGTCACACCAg                                                    >  2:799618/1‑141 (MQ=255)
                                                                                  gTATTCTTATTTTCTTGGTGTTCTTTAATCCATTGGTTAATTTGCTCAGGTGAAATTTCATTAGCTGATTTCGACTCTTTCGCCATTATTTCGCACCTGCTCTTTTTTTATATACTTAGTCATACTGATTGTCACACCAGa                                                   >  2:668098/1‑141 (MQ=255)
                                                                                  gTATTCTTATTTTCTTGGTGTTCTTTAATCCATTGGTTAATTTGCTCAGGTGAAATTTCATTAGCTGATTTCGACTCTTTCGCCATTATTTCGCACCTGCTCTTTTTTTATATACTTAGTCATACTGATTATCACACCAgt                                                   >  2:310141/1‑140 (MQ=255)
                                                                                   tATTCTTATTTTCTTGGTGTTCTTTAATCCATTGGTTAATTTGCTCAGGTGAAATTTCATTAGCTGATTTCGACTCTTTCGCCATTATTTCGCACCTGCTCTTTTTTTCTATACTTAGTCCTACTGATTGTCACGCCAGAt                                                  >  2:781059/1‑141 (MQ=255)
                                                                                   tATTCTTATTTTCTTGGTGTTCTTTAATCCATTGGTTAATTTGCTCAGGTGAAATTTCATTAGCTGATTTCGACTCTTTCGCCATTATTTCGCACCTGCTCTTTTTTTATATACTTAGTCCTTCTGATTGTCCCACCAAAt                                                  >  2:805693/1‑141 (MQ=255)
                                                                                        tttttttCTTGGGTTTCTTTAATCCATTGGTTAATTTGCTCACGTGAAATTTCATTAGCTGATTTCGACTCTTTCGCCATTATTTCGCACCTGCTCTTTTTTTATATACTTAGTCATACTGATTGTCACACCAGATTCttt                                             <  2:97350/138‑1 (MQ=255)
                                                                                        ttATTTTCTTGTTGTTCTTTAATCCATTGGTTAATTTGCTCCTGGTATCTTTCATTAGTTTATTTCGACTCTTTCGCCTTTTTTTCTCACCTGCTCTGTTTTTATATACTTAGTCCGACTTAATGGCACACCAAATTCtat                                             <  2:349991/141‑3 (MQ=255)
                                                                                        ttATTTTCTTGGTGTTCTTTAATCCATTGGTTAATTTGCTCAGGTGAAATTTCATTAGCTGATTTCGGCTCTTTCGCCATTATTTCGCACCTGCTCTTTTTTTATTTACTTGGTCATGCTGTTTGTCACATCATTTTCttt                                             <  2:274141/141‑1 (MQ=255)
                                                                                        ttATTTTCTTGGTGTTCTTTAATCCATTGGTTAATTTGCTCAGGTGAAATTTCATTAGCTGATTTCGACTCTTTCGCCATTATTTCGCACCTGCTCTTTTTTTATATACTTAGTCATACTGATTGTCACACCAGATTCttt                                             <  2:351741/141‑1 (MQ=255)
                                                                                        ttATTTTCTTGGTGTTCTTTAATCCATTGGTTAATTTGCTCAGGTGAAATTTCATTAGCTGATTTCGACTCTTTCGCCATTATTTCGCACCTGCTCTTTTTTTATATACTTAGTCATACTGATTGTCACACCAGATTCttt                                             <  1:296064/141‑1 (MQ=255)
                                                                                            tttCTTGGTGTTCTTTAATCCATTGGTTAATTTGCTCAGGTGAAATTTCATTAGCTGGTTTCGCCTCTTTCGCCATTATTTCGCACCTGCTCTTTTTTTATATACTTAGTCATACTGATTGTTACACCAGATTCTTtatat                                         >  1:652602/1‑141 (MQ=255)
                                                                                            tttCTTGGTGTTCTTTAATCCATTGGTTAATTTGCTCAGGTGAAATTTCATTAGCTGATTTCGACTCTTTCGCCATTATTTCGCACCTGCTCTTTTTTTATATACTTAGTCATACTGATTGTCACAGCAGATTCTTtatat                                         >  1:207883/1‑141 (MQ=255)
                                                                                            tttCTTGGTGTTCTTTAATCCATTGGTTAATTTGCTCAGGTGAAATTTCATTAGCTGATTTCGACTCTTTCGCCATTATTTCGCACCTGCTCTTTTTTTATATACTTAGTCATACTGATTGTCACACCAGATTCTTtatat                                         >  1:734565/1‑141 (MQ=255)
                                                                                             ttCTTGGTGTTCTTTAATCCATTGGTTAATTTGCTCAGGTGAAATTTCATTAGCTGATTTCGACTCTTTCGCCATTATTTCGCACCTGCTCTTTTTTTATATACTTAGTCATACTGATTGTCACACCAGATTCTTtatata                                        <  1:617820/141‑1 (MQ=255)
                                                                                             ttCTTGGTGTTCTTTAATCCATTGGTTAATTTGCTCAGGTGAAATTTCATTAGCTGATTTCGACTCTTTCGCCATTATTTCGCACCTGCTCTTTTTTTATATACTTAGTCATACTGATTGTCACACCAGATTCTTtatata                                        <  1:611100/141‑1 (MQ=255)
                                                                                                ttGGTGTTTTTTAATCCATTGTTTAATTTTCTCAGCTGAAATCTCATTAGCTGATTTCGACTCTTTCGCCATTATTTCGCACCTGCTCTTTTTTTATATACTTAGTCATACTGATTGTCACACCAGATTCTTTATATACtg                                     <  2:488499/141‑1 (MQ=255)
                                                                                                ttGGTGTTCTTTAATCCTTTGGTTAATTTGCTCAGGTGAAATTTCATTAGCTGTTTTCGACTCTTTCGCCATTATTTCGCACCTGCTCTTTTTTTATATACTTAGTCATACTGATTGTCACACCAGATTCTTTATATACtg                                     <  2:37660/141‑1 (MQ=255)
                                                                                                    tgtTCTTTAATCCATTGGTTAATTTGCTCAGGTGAAATTTCATTAGCTGATTTCTACTCTTTCGCCATTATTTCGCACCTGCTCTTTTTTTTTTTACTTTGTCATACTGATTGTCACGCCAGATTCTTTATATCCTGTGAc                                 >  2:153954/1‑141 (MQ=255)
                                                                                                        tttttATCCATTGGTTAATTTGCTCAGGTGAAATTTCATTAGCTGATTTCGACTCTTTCGCCATTATTTCGCACCTGTTCTTTTTTTATATACTTAGTCATACTGATTGTCACACCAGATTCTTTATATACTGTGACTTCa                             <  2:353796/140‑1 (MQ=255)
                                                                                                              tCCATTGGTTAATTTGCTCAGGTGAAATTTCATTAGCTGATTTCGACTCTTTCGCCATTATTTCGCACCTGCTATCTTCGGAGATTCTTAGTCATACTGATTGTCACACCAGATTCTTTATATACTGTGACTTCATCCAtt                       >  1:793176/1‑141 (MQ=255)
                                                                                                               ccATTGGTTAATTTGCTCAGGTGAAATTTCATTAGCTGATTTCGACTCTTTCGCCATTATTTCGCACCTGCTCTTTTTTTATATACTTAGTCATACTGATTGTCACACCAGATTCTTTATTTACTTTGACTTCATCCATTa                      >  2:702890/1‑141 (MQ=255)
                                                                                                                 aTTGGTTAATTTGCTCAGGTGAAATTTCATTAGCTGATTTCGACTCTTTCGCCATTATTTCGCACCTGCTCTTTTTTTATATACTTAGTCATACTGATTGTCACACCAGATTCTTTATATACTGTGACTTCATCCATTaaa                    >  1:769232/1‑141 (MQ=255)
                                                                                                                  gtGGTTAATTTGCTCAGGTGAAATTTCATTAGCTGATTTCGACTCTTTCGCCATTATTTCGCACCTGCTCTTTTTTTATATACTTAGTCATACTGATTGTCACACCAGATTCTTTATATACTTTGACTTCATCCATTAAAg                   <  2:492708/140‑1 (MQ=255)
                                                                                                                    ggTTAATTTGCTCAGGTGAAATTTCATTAGCTGATTTCGACTCTTTCGCCATTATTTCGCACCTGCTCTTTTTTTATATACTTAGTCATACTGATTGTCACACCAGATTCTTTATATACTGTGACTTCATCCATTAAAGAt                 <  1:362679/141‑1 (MQ=255)
                                                                                                                         aTTTGCTCAGGTGAAATTTCATTAGCTGATTTCGACTCTTTCGCCATTATTTCGCACCTGCTCTTTTTTTATATACTTAGTCATACTGATTGTCACACCAGATTCTTTATATACTGTGACTTCATCCATTAAAGATTcagt            >  2:647368/1‑138 (MQ=255)
                                                                                                                         aTTTGCTCAGGTGAAATTTCATTAGCTGATTTCGACTCTTTCGCCATTATTTCGCACCTGCTCTTTTTTTATATACTTAGTCATACTGATTGTCACACCAGATTCTTTATATACTGTGACTTCATCCATTAAAGATTCGAt            >  1:384273/1‑141 (MQ=255)
                                                                                                                         aTTTGCTCAAGTTAAATTTCATTAGCTGATTTAGACCCGGTCGCCATTATTTCGTCCCCATTGTTTATTTATATACTTAGTCATACTGATTGTCACACCAGATTCTTTATATACTGTGACTTCATCCATTAAAGATTCGAt            >  2:170507/1‑141 (MQ=255)
                                                                                                                          tttGCTCAGGTGAAATTTCATTAGCTGATTTCGACTCTTTCGCCATTATTTCGCACCTGCTCTTTTTTTATATACTTAGTCATACTGATTGTCAGACCAGATTCTTTATATACTGTGACTTCATCCATTAAagattagatt           >  1:804836/1‑141 (MQ=255)
                                                                                                                              cTCAGGTGAAATTTCATTAGCTGATTTCGACTCTTTCGCCATTATTTCGCACCTGCTCTTTTTTTATATACTTAGTCATACTGATTGTCACACCAGATTCTTTATATCCTGTGACTTCATCCATTAAAGATTCGATTaaaa       >  2:631493/1‑141 (MQ=255)
                                                                                                                                  ggTGTAATTTCTTTAGTTTATTTCGACTTTTTCGCCATTATTTCTCACCTGCTCTTTTTTTATATATTTAGTCATACTGATTGTCACACCAGATTCTTTATATACTGTGACTTCATCCATTAAAGATTCGATTAAAAATaa   <  2:640333/141‑1 (MQ=255)
                                                                                                                                   gTGAAATTTCATTAGCTGATTTCGACTCTTTCGCCATTATTTCGCACCTGCTCTTTTTTTATATACTTAGTCATACTGATTGTCACACCAGATTTTTTATATACTGTGACTTCATCCATTGAGGTTTCGattaaaattaaa  >  1:711000/1‑141 (MQ=255)
                                                                                                                                     |                                                                                                                                          
ATGTGATTGTCCTTTAGAATATTTATATGCCAATGACTCAATTAGTTTTTGGTAATGTTTAACTAACTTATCCTGTGCATCTGTATTCTTATTTTCTTGGTGTTCTTTAATCCATTGGTTAATTTGCTCAGGTGAAATTTCATTAGCTGATTTCGACTCTTTCGCCATTATTTCGCACCTGCTCTTTTTTTATATACTTAGTCATACTGATTGTCACACCAGATTCTTTATATACTGTGACTTCATCCATTAAAGATTCGATTAAAAATAAA  >  CP000730/2185993‑2186264

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: