Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 110,651 C→T 10.5% D170D (GAC→GAT USA300HOU_0109 → staphylococcal tandem lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730110,6510CT10.5% 292.7 / 21.4 120D170D (GAC→GATUSA300HOU_0109staphylococcal tandem lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (45/60);  new base T (6/6);  total (51/69)
Fisher's exact test for biased strand distribution p-value = 7.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.88e-01

Only 100 of 122 total aligned reads displayed.

AAAAGATATCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGTT  >  CP000730/110511‑110790
                                                                                                                                            |                                                                                                                                            
aaaaGATATCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCCATATGGCGCCTTTACACATTTGAAAAATTATAAacac                                                                                                                                              >  1:662003/1‑141 (MQ=18)
  aaGATATCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGAcaa                                                                                                                                            >  1:217009/1‑139 (MQ=255)
   aGATATCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGga                                                                                                                                           >  2:777789/1‑141 (MQ=255)
    gATATCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGgag                                                                                                                                         <  1:357589/141‑1 (MQ=255)
    gATATCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGgag                                                                                                                                         <  1:488177/141‑1 (MQ=255)
     atatCCGGATAAAATGATAGATAATAAAATCATTCCAACAAAAAAAATTAGATATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGATTTTAAAAATTTGAAAAATTATAAAGACGgaga                                                                                                                                        <  2:726067/141‑1 (MQ=18)
     aaatgccgTTAAAATGTACAATCATAAAATAATCCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTCTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGgaga                                                                                                                                        <  1:142883/134‑1 (MQ=17)
      tatCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGgagaa                                                                                                                                       >  2:475548/1‑140 (MQ=255)
      tatCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGgagaa                                                                                                                                       >  2:475626/1‑140 (MQ=255)
      tatCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGat                                                                                                                                       >  1:50183/1‑141 (MQ=255)
         ccGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATAtt                                                                                                                                    >  2:67261/1‑141 (MQ=255)
          cGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGTCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATAttt                                                                                                                                   <  1:799456/141‑1 (MQ=255)
           ggTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAAGAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTc                                                                                                                                  <  1:284083/141‑1 (MQ=255)
            gTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCa                                                                                                                                 <  1:3697/141‑1 (MQ=255)
              taaattgtacataaataaAACCATTCAACAAAAACAAACTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTACGTTCGCTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCata                                                                                                                               <  1:237650/128‑1 (MQ=17)
               aaaaTGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCatat                                                                                                                              <  1:777789/141‑1 (MQ=255)
                              aaaaTCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAATAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATTCAGAGGTGcca                                                                                                               >  1:77981/1‑140 (MQ=255)
                                      ttcaacaaAATAAATTAAATATGAAAAAATAAAAAAAGAAATCGAAAACTTTAATTTCTTTGTTCAATATGCTGACTTTAAAAATTTGAAAAATTATAAAGACTGAGATATTTCATATAATCCAGAGGTGCTGAGTTATTc                                                                                                       <  2:529994/139‑1 (MQ=17)
                                      tCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTc                                                                                                       <  1:134247/141‑1 (MQ=255)
                                      tCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGGTATTc                                                                                                       >  2:46654/1‑141 (MQ=255)
                                      ccaaacaaAAAAAAATAAAGAAAAAAAAAAAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATTGCGACTTTAAAAATTTGAAAAATTATAAAGACGTAGATATTTCATATAATCCAGAGGTGCCGAGTTATTc                                                                                                       <  2:374904/138‑1 (MQ=17)
                                       ccAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGGGGTGCCGAGTTATTCg                                                                                                      <  1:402185/141‑1 (MQ=255)
                                        caacaaAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTGAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCgg                                                                                                     >  1:546435/1‑141 (MQ=255)
                                          acaaACGAACTGACATATGAACAAATAAAAAAAGAAACACAAAACTTTAAGTTCTTTTTTCAATATGCCGCCTTTACTAATTTGCAAAATTATAAAGCCGGAGATTCTTCATATAATCCAGAGGTGCCCAGTTATTCGGCt                                                                                                   <  2:350541/141‑1 (MQ=12)
                                           aaaacaaaaTTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTa                                                                                                  <  2:511663/135‑1 (MQ=255)
                                               aGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAata                                                                                              <  1:627690/141‑1 (MQ=255)
                                               aGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAata                                                                                              <  1:35134/141‑1 (MQ=255)
                                                 aaaTTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTGCTGTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAatata                                                                                            >  1:693011/1‑140 (MQ=255)
                                                  aaataaaaataaaaaAAAAAAAAAAGAAATGGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCa                                                                                           <  2:189165/138‑1 (MQ=17)
                                                  aaTTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAGGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCa                                                                                           <  2:166587/141‑1 (MQ=255)
                                                  aaTTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCa                                                                                           >  2:755934/1‑141 (MQ=255)
                                                   aTTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCaa                                                                                          >  2:295878/1‑141 (MQ=255)
                                                     tAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAAtt                                                                                        >  1:275951/1‑141 (MQ=255)
                                                     tAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAAtt                                                                                        >  1:496749/1‑141 (MQ=255)
                                                     gaaaGATGAAAAAATAAAAAAAGAAATCGAAAACACTAAGTTCTTGGATCAATATGACGACATTAAAAAATGGAAAAAGTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCAGAGTTATAAGGCTAAATATCAAtt                                                                                        >  1:477331/2‑141 (MQ=17)
                                                       aaGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTaa                                                                                      >  1:598847/1‑141 (MQ=255)
                                                       aaGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTaa                                                                                      >  1:635236/1‑141 (MQ=255)
                                                       aaGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTaa                                                                                      >  1:573076/1‑141 (MQ=255)
                                                        aGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTACCGAGTTATTCGGCTAAATATCAATTAAc                                                                                     >  1:365559/1‑141 (MQ=255)
                                                         gATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATGTAATGAAGAGGTGCCGGGTTATTCGGCTAAATATGAATTaatt                                                                                    >  1:13131/1‑139 (MQ=17)
                                                         gATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACt                                                                                    >  2:500887/1‑141 (MQ=255)
                                                            gAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATACGGGTGCTTTAAATAGATGAAACACTATGATGCAGGTCATAGTTCATCTAATCCAGAGGTGCCGAGTTATTCGGGTAAGTATCAATTAAcgtat                                                                                 <  2:332800/141‑5 (MQ=9)
                                                                     aaaacaGAAATCGAAAACTTGAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATtat                                                                        <  2:172043/141‑1 (MQ=255)
                                                                     aaaaaaGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTACATATCAATTAACTAATGATGATtaa                                                                        >  1:41010/1‑140 (MQ=255)
                                                                     aaaaaaGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATtat                                                                        >  1:227237/1‑141 (MQ=255)
                                                                     aaaaaaGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATtat                                                                        <  1:408823/141‑1 (MQ=255)
                                                                              aTCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTaaaa                                                               <  2:665778/141‑1 (MQ=255)
                                                                                cGAAAACTTTAAGTTCTTTCTTCAATATGGCGACTTTAAAAATTTGACAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTCACTAATGATGATTATAATGTacaaca                                                             >  1:484484/1‑141 (MQ=255)
                                                                                cGAAAACTTCAAGTTCTGTTTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACa                                                             <  1:640079/141‑1 (MQ=255)
                                                                                  agagctgtgaGTGCTGTGTTGAATAGGGCGACTTTAAAAATTTGAATAATTAGAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTGATTCGGCTAAAGATCAATTAACTAATGATGATTATAATGTAAAACAAt                                                           <  2:662003/132‑1 (MQ=17)
                                                                                  aacattttAAGTTCTTTGTTGAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAAt                                                           <  1:94714/136‑1 (MQ=255)
                                                                                  aaaaCTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATCTAGAGGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAAGGATGATTATAATGTAAAACAAt                                                           <  1:385097/141‑1 (MQ=255)
                                                                                  aaaaCTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAAt                                                           <  1:249293/141‑1 (MQ=255)
                                                                                     aCTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTAc                                                        <  2:692915/141‑1 (MQ=255)
                                                                                     aCTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTAc                                                        <  2:50183/141‑1 (MQ=255)
                                                                                       tttAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATTGCATCCAGAGGTGCCTAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGc                                                      <  1:591240/141‑1 (MQ=255)
                                                                                        ttAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGGTATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTCACTAATGATGATTATAATGTAAAAGAATTACGCa                                                     >  2:460215/1‑141 (MQ=255)
                                                                                        ttAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTATCTCAAGTGAAGTAGAATGTAAAACAATTACGCa                                                     <  1:800300/141‑1 (MQ=17)
                                                                                         tAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCaa                                                    <  1:313507/141‑1 (MQ=255)
                                                                                         tAAGGTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCaa                                                    <  1:781833/141‑1 (MQ=255)
                                                                                              tCTTTGTTCAATATTGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAgat                                               <  1:576695/141‑1 (MQ=255)
                                                                                               cgttgatgaaTATGGCGACTTTAAATATTTGTGAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAgata                                              <  2:726513/133‑1 (MQ=14)
                                                                                               cTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGATGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAgata                                              <  2:327589/141‑1 (MQ=255)
                                                                                                    ttCAATATGGCGCATTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAgatatgata                                         <  1:56429/141‑1 (MQ=255)
                                                                                                    ttCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAgatatgata                                         <  2:57665/141‑1 (MQ=255)
                                                                                                    gcaaaTATGGCGACTTTAAAAATTTGAAAAATTATAAATACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAgatatgata                                         <  2:277069/138‑1 (MQ=255)
                                                                                                      cAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTCATGATGATTATAATGTAGAACAATTACGCAAAAGATATGAtgta                                       >  1:164550/1‑141 (MQ=255)
                                                                                                          atGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAGCAATTACGCAAAAGATATGATATACcga                                   >  1:134881/1‑141 (MQ=255)
                                                                                                            ggCGACTTTAAAAATTTGAATCACTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACcgacg                                 >  2:779805/1‑141 (MQ=255)
                                                                                                            ggCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACcgacg                                 >  2:582673/1‑141 (MQ=255)
                                                                                                               gACTTTAAAAATTTGAAAAATTTTAAAGACGGATATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGt                              >  1:393610/1‑141 (MQ=255)
                                                                                                               gACTTTAAAAATTTGAAAAATTATAAAGACGGAGCTATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACGAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAga                              >  2:191114/1‑140 (MQ=255)
                                                                                                               gACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGt                              >  2:362134/1‑141 (MQ=255)
                                                                                                                 cTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTaa                            <  1:641084/141‑1 (MQ=255)
                                                                                                                   ttAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAg                          >  1:529578/1‑141 (MQ=255)
                                                                                                                     aaaaaTTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCt                        >  1:375650/1‑141 (MQ=255)
                                                                                                                     aaaaaTTTGAAAAATTATAAAATGAATTAGATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCt                        >  1:194434/1‑141 (MQ=17)
                                                                                                                      aagatttaatgaaTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTc                       >  2:722221/12‑141 (MQ=11)
                                                                                                                      aaaaTTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTc                       >  2:200674/1‑141 (MQ=255)
                                                                                                                      aaaaTTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATAACGCAAAAGATATGATATACCGACGAGTAAAGCTc                       >  2:442990/1‑141 (MQ=255)
                                                                                                                          tttGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCaaa                   <  1:353395/141‑1 (MQ=255)
                                                                                                                              atgaaTGATAAAGAATGAGATATAACATAAAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTa               <  1:599419/138‑1 (MQ=17)
                                                                                                                               aaaaTTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTACTGATGATTGTATGGTAAATCAATTACGCATAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTAt              <  2:369677/141‑1 (MQ=18)
                                                                                                                                aaaTTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAGCAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTAtt             >  2:551019/1‑141 (MQ=255)
                                                                                                                                aaaTTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGGTATACCGACGAGTCAAGCTCCAACGtaaat             >  2:281085/1‑137 (MQ=255)
                                                                                                                                  aTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGt           <  1:358033/141‑1 (MQ=255)
                                                                                                                                  aTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGt           <  1:805402/141‑1 (MQ=255)
                                                                                                                                  aTTATAAAGATGGAGATACTCCTCTAAAGCCAGATGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGt           <  2:564730/141‑1 (MQ=18)
                                                                                                                                  aTTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACGGACGAGTAAAGCTCCAAAGTTATTGt           >  2:700818/1‑141 (MQ=255)
                                                                                                                                  aTTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGt           <  2:217009/141‑1 (MQ=255)
                                                                                                                                     ataAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGTGTAAAGCTCCAAAGTTATTGTTaa        >  2:505779/1‑141 (MQ=255)
                                                                                                                                     ataAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATGGTTaa        >  2:718964/1‑141 (MQ=255)
                                                                                                                                      taAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTaaa       <  2:371016/141‑1 (MQ=255)
                                                                                                                                      taAAGACGGAGATATTTCATACAATCCAGAGGTTCCGCGTTTCTGAAATCGCAATGGAGTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTaaa       <  1:584456/141‑1 (MQ=18)
                                                                                                                                      taAAGACGGAGATATTACATATAACCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTaaa       <  2:761968/141‑1 (MQ=255)
                                                                                                                                        aaGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAg     >  1:794683/1‑141 (MQ=255)
                                                                                                                                        aaGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATGGTTAAAAg     >  1:468979/1‑141 (MQ=255)
                                                                                                                                         aGACGGAGGTATATCCTATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATCAACTAACGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAgg    <  2:316106/141‑1 (MQ=18)
                                                                                                                                         aGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAgg    <  1:284730/141‑1 (MQ=255)
                                                                                                                                           aCGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGtt  <  2:235961/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                            
AAAAGATATCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGTT  >  CP000730/110511‑110790

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: