BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	2,775,053	A→G	6.6%	D711D (GAT→GAC)	clfB ←	clumping factor B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,775,053	0	A	G	6.6%	233.5 / 2.7	99	D711D (GAT→GAC)	clfB	clumping factor B
Reads supporting (aligned to +/- strand): ref base A (39/49); new base G (3/4); total (44/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 112 total aligned reads displayed.

GAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAATCGCTATCTGAGTCTGAATCACTGTCTGAGTCCGAGTCACTG  >  CP000730/2774913‑2775191

gcaccGCTTTCTGCTTCCCATTCGACATCTGAATCTGACGCGCTCTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaa                                                                                                                                            <  2:786766/137‑1 (MQ=14)

  atcGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTGTGAGTCTGAATCGCTatctgaatc                                                                                                                                          >  2:327618/1‑141 (MQ=255)

  atcGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaatc                                                                                                                                          <  1:559922/141‑1 (MQ=255)

   tcGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaatct                                                                                                                                         >  1:644923/1‑141 (MQ=255)

    cGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaatctg                                                                                                                                        >  1:747996/1‑141 (MQ=255)

    cGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGCCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGACTGAGTCTGAGTCGCTGTCTGAGTCTGCATCGCTatctgaaccgg                                                                                                                                        >  1:277363/1‑137 (MQ=255)

     ccTGTCTGAGTCTGATTCGCTTTCTGAATCTGAGTCGCTGTCTGAGTCTGACTCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaatctga                                                                                                                                       <  2:278070/140‑1 (MQ=255)

      cTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAGTCGGAGTCGCTATCTGGGTCTGAGCCGCTGTCTGAGGCTGAGACGCTGTCTGAGTCTGAATCGCTGTCTAAATCTGAg                                                                                                                                      >  2:108504/1‑141 (MQ=14)

      cTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGGATCGCTgtctgcgtctggg                                                                                                                                      >  2:455861/1‑139 (MQ=255)

      cTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAg                                                                                                                                      >  1:553614/1‑141 (MQ=255)

        gTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTc                                                                                                                                    >  1:675975/1‑141 (MQ=255)

         tCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTGTGAGTCGCTGACTGAGCGTGAGTCGCTATCTGAGTGTGACTCGGTATCTGACTCGGAGTCg                                                                                                                                   >  2:529760/1‑141 (MQ=14)

           tGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCt                                                                                                                                 <  1:639518/141‑1 (MQ=255)

              gTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTgtc                                                                                                                              <  1:271680/141‑1 (MQ=255)

               tCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAGTCGCTgtct                                                                                                                             >  2:435334/1‑141 (MQ=255)

                        ctgtctgactctgaGTCGCTGTCCGCGTCTGAATCTCTTCCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTTTCTGAGTCTGAGTCGCTATCTGAATCTGAGTCGCTGtctgagtctgag                                                                                                                    <  2:406630/138‑1 (MQ=11)

                           tctgaatctgaGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCGGAGTCGCTGTCTGAATCTGAGTCGCTATCTGAGTCTGAATCACTATCTGAGTCTGAGTCGCTGTCTGAAtctgagtcg                                                                                                                 >  2:710476/1‑141 (MQ=11)

                            ctgaatctgaGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgc                                                                                                                <  1:574016/141‑1 (MQ=255)

                              gaatctgaGTCGCTGTCTGGGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctg                                                                                                              <  1:601344/141‑1 (MQ=255)

                              gaatctgaGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctg                                                                                                              <  1:724936/141‑1 (MQ=255)

                               aatctgaGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAGCTGAGTCTGAGTCGCTGTCTGAGTCTGAGGCGCTATCTGAGTCTGAATAGCTGTCTGAATCTGAGTCTGAGTCTGAGtctgaggcgatgg                                                                                                             >  2:715814/1‑140 (MQ=14)

                                acctgaGTCGCTGTCTTAGTCTGAATCGCTATCTGAATCTGAGTCGCTTTCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtc                                                                                                            <  2:617768/139‑1 (MQ=255)

                                  ctgaGTCCCTGTCTTGGTCTGAATTGCTGCCTGCATCGGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGACTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctg                                                                                                          <  2:479249/141‑1 (MQ=14)

                                    gaGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaa                                                                                                        <  1:781882/141‑1 (MQ=255)

                                     aGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAATCGCTATCTGAAACTGAATCGATGTCTGGCTCTGAATCGCTATCTGATTCTGAATCGCCggctgagtctgaaacgcgatctgcaa                                                                                                       >  2:466442/1‑127 (MQ=11)

                                      gTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGCGtatgagtcgcagtatgatca                                                                                                      >  2:398545/1‑138 (MQ=17)

                                      gTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGACTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgcgggctgaatc                                                                                                      >  2:794597/1‑141 (MQ=255)

                                        cGCTGTCTGAGTCTGAATCGCTATCTGACTCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctg                                                                                                    <  1:765579/141‑1 (MQ=255)

                                         ccTGTCTGAATCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctga                                                                                                   <  1:179891/140‑1 (MQ=255)

                                          cTGTCTGAGTCTGAGTCGCGATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctgag                                                                                                  <  2:145807/141‑1 (MQ=255)

                                          cTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTGTCGGAGtctgaggcgctgtctgagtttgag                                                                                                  >  2:710225/1‑141 (MQ=14)

                                          cTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtgtgaagcgctgtctgagtgtgac                                                                                                  >  2:124745/1‑140 (MQ=14)

                                             tctgagtctgaATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctgagtcg                                                                                               <  1:305636/141‑1 (MQ=255)

                                             tctgagtctgaATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGCCGCTGTCTGAGtctgagtcgcggtctgaatctgagtcg                                                                                               <  1:452071/141‑1 (MQ=255)

                                                gattcggaATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctgagtcgctg                                                                                            <  2:485544/135‑1 (MQ=255)

                                                gagtctgaATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctgagtcgctg                                                                                            >  1:649106/1‑141 (MQ=255)

                                                 agtctgaATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctgagtcgctgt                                                                                           <  2:627763/141‑1 (MQ=255)

                                                  gtctgaATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctgagtcgctgtc                                                                                          >  1:467906/1‑141 (MQ=255)

                                                   tctgaATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctgagtcgctgtct                                                                                         <  1:740547/141‑1 (MQ=255)

                                                   tctgaATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCCGACTTTGAGGCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctgagtcgctgtct                                                                                         <  1:756375/141‑1 (MQ=255)

                                                    ctgaATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctgagtcgctgtctg                                                                                        <  1:765945/141‑1 (MQ=255)

                                                      gaATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctgagtcgctgtctgaa                                                                                      <  1:509838/141‑1 (MQ=255)

                                                      gaACCGCTATCTTCATCTGAGTCGCCATCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctgagtcgctgtctgaa                                                                                      <  2:48100/141‑1 (MQ=14)

                                                          cccTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGagtcgctgtctgaatctgagtcgctgtctgaatctg                                                                                  <  1:744410/139‑1 (MQ=255)

                                                          cGCTGTCTGATTCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCGATCTGACTCTGAGTCGCTGTCTGAGTCTGagtcgctgtctgaatctgagtcgctgtctgaatctg                                                                                  <  1:529760/141‑1 (MQ=255)

                                                          cGCTATCTGTATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGagtcgctgtctgaatctgagtcgctgtctgaatctg                                                                                  <  1:87412/141‑1 (MQ=255)

                                                          cGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGagtcgctgtctgaatctgagtcgctgtctgaatctg                                                                                  <  1:440650/141‑1 (MQ=255)

                                                              ttctgattctgaGTCGCTGTCTGAGTCTGAGGCGCTGTCTCATTTTGAGTCGCTGTCTGAGGCTGATTCGCTGTCTGAGGCTGAGTCGTTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCtgaatctgaatc                                                                              <  2:362174/140‑1 (MQ=11)

                                                              atctgaatctgaGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTTAGTCGCTGTCtgaatctgaatc                                                                              <  1:194895/141‑1 (MQ=255)

                                                                ctgaatctgaGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGACTCGc                                                                            <  1:550713/141‑1 (MQ=255)

                                                                ccgaatcggaTTCTCTATCTGAGTCTGAGTCGCTCTCTGAGTCTGAGTCCCTGTCTGAGTCTGAATCGCTTTCTGAATCTGAGTCGCTTTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGc                                                                            <  2:388962/139‑1 (MQ=14)

                                                                 tgaatctgaGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCt                                                                           >  2:36671/1‑141 (MQ=255)

                                                                 tgaatctgaGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCGGTCTGAATCTGAATCGCt                                                                           >  1:674612/1‑141 (MQ=255)

                                                                  gaacctgaGTCGCGCTCCGAGTCGGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTg                                                                          <  2:671374/141‑1 (MQ=17)

                                                                   aatctgaGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTgt                                                                         <  1:86369/141‑1 (MQ=255)

                                                                   aatctgaGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTCAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCCGTCTGAATCTGAATCGCTgt                                                                         >  1:597859/1‑141 (MQ=255)

                                                                     tctggGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTgtct                                                                       <  1:248083/141‑1 (MQ=255)

                                                                     tctgaGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTgtct                                                                       <  1:353170/141‑1 (MQ=255)

                                                                     tctgaGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTgtct                                                                       <  1:116321/141‑1 (MQ=255)

                                                                      ctgaGTCGCTGTCTGAGTCGGAGTCCCCGTCTGTGTCTGAGTCGCTGTCTGAGTCTGACTCGCTATCTGAATCTGCGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTgtctg                                                                      <  2:404451/141‑1 (MQ=17)

                                                                        gaGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTgtctgag                                                                    >  1:607123/1‑141 (MQ=255)

                                                                        gaGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGGCGGTGTCTGAAGTTGAATCGCTgtctgag                                                                    >  1:566113/1‑141 (MQ=255)

                                                                          gTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGCATCTGAGTCGCTGTCTGAATCTGAATCGCTgtcttagtc                                                                  <  2:675975/141‑1 (MQ=255)

                                                                           tCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTgtctgagtct                                                                 >  1:807497/1‑141 (MQ=255)

                                                                           tCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTgtctgagtct                                                                 >  1:66062/1‑141 (MQ=255)

                                                                              cTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAGTCGCTgtctgagtcagca                                                              >  2:77964/1‑137 (MQ=255)

                                                                                aTCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGtctgagtctgaatc                                                            <  1:108504/141‑1 (MQ=255)

                                                                                aTCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGtctgagtctgaatc                                                            <  1:455861/141‑1 (MQ=255)

                                                                                aTCTGAGTCCGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGtctgagtctgaatc                                                            <  1:705339/141‑1 (MQ=255)

                                                                                aTCGGAGTCTGAGTGGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGtctgagtctgaatc                                                            <  1:230605/141‑1 (MQ=255)

                                                                                   tgagtctgagtcgctgtctgagtctgagtcgctgtcGGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGtctgagtctgaatcgct                                                         <  1:449834/141‑1 (MQ=255)

                                                                                    gagtctgagtcgctgtctgagtctgagtcgctgtctGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTGTGCATCTGAATCGCTGTCTGAATCGGAGTCGCTAtctgagtctgaatcgcgc                                                        >  2:350245/1‑139 (MQ=14)

                                                                                    gagtctgagtcgctgtctgagtctgagtcgctgtctGAGACTGAATCACTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGtctgagtcggaatctcta                                                        >  2:506197/1‑141 (MQ=14)

                                                                                          gagtcgctgtctgagtctgagtcgctgtctgagtctGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAAtctgaatcgctgtctgagtctgaatcgctgtctgag                                                  >  2:645444/1‑141 (MQ=255)

                                                                                          gagtcgctgtctgagtctgagtcgctgtctgagtctGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGtctgagtctgaatcgctatctgag                                                  >  1:521347/1‑141 (MQ=255)

                                                                                           agtcgctgtctgagtctgagtcgctgtctgagtctgAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGtctgagtctgaatcgctatctgagt                                                 >  2:413894/1‑141 (MQ=255)

                                                                                                ctgtctgagtctgagtcgctgtctgagtctgaATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGtctgagtctgaatcgctatctgagtctgta                                            >  2:736401/1‑139 (MQ=255)

                                                                                                  gtctgagtctgagtcgctgtctgagtctgaATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGtctgagtctgaatcgctatctgagtctgaatc                                          <  1:516834/141‑1 (MQ=255)

                                                                                                   tctgagtctgagtcgctgtctgagtctgaATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATGTGAGACGCTGTCTGAATCTGAATGACTGtctgagggtgaagcgctatgtgatacgagatcg                                         >  2:430189/1‑131 (MQ=17)

                                                                                                   tctgagtctgagtcgctgtctgagtctgaATCGCTATCTGAATCTGAGTCGCTGTATGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAATCTGAATCGCTGtctgactctgaatcactatctgactccgaatcc                                         >  2:605580/1‑140 (MQ=17)

                                                                                                     tgagtctgagtcgctgtctgagtctgaATCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGtctgagtctgaatcgctatctgagtctgaatcgct                                       <  2:492317/141‑1 (MQ=255)

                                                                                                         tctcagtcgctttctgagtcttcTTCGCTATCTGAGTCTTAGTCGCCGTCTGCGTCTGAGTCGCTATCTGAATCCTAGTCGCTGTCTGATTCTGCATCGCTATCTgtgtctgaatcgcgatctgagtctgaatcgctatct                                   <  2:13139/141‑1 (MQ=14)

                                                                                                              gtcgctgtctgagtctgaATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGACTCTGAATCGCTGTCTGAGTCtgaatcgctatctgtgtctgaatcgatttctgactc                              >  2:382308/1‑141 (MQ=17)

                                                                                                              gtcgctgtctgagtctgaATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCtgaatcgctatctgagtctgaatcgctatctgagtc                              <  1:327618/141‑1 (MQ=255)

                                                                                                               tcgctgtctgagtctgaATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTgaatcgctatctgagtctgaatcgctatctgagtct                             >  1:226473/1‑141 (MQ=255)

                                                                                                                cgctgtctgagtctgaATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGaatcgctatctgagtctgaatcgctatctgagtctg                            <  1:231194/141‑1 (MQ=255)

                                                                                                                  ctgtctgagtctgaATCGCTATCTGAATCTGAGTCGCTGTATGAGTCTGAGTCGCTGTCTGAATCTGAGTCGATGTCTGAATCTGAGTCGCTGTCTGAGTCTGAAtcgctatctgagtctgcatagctatctgagtttgaa                          >  2:212551/1‑141 (MQ=17)

                                                                                                                    gtctgagtctgaATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCgctatctgagtctgaatcgctatctgagtctgaatc                        >  1:154062/1‑141 (MQ=255)

                                                                                                                    gtctgagtctgaATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCgctatctgagtctgaatcgctatctgagtctgaatc                        >  1:365298/1‑141 (MQ=255)

                                                                                                                    gtctgagtctgaATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCgctatctgagtctgaatcgctatctgagtctgaatc                        >  1:312142/1‑141 (MQ=255)

                                                                                                                      ctgagtctgaATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGGGTCTCGATCTGTATACGACTCGCTGTCGGAGtctgcatcgcgagatgagtcagaatcgctgtctgaggcaggaacac                      >  2:46325/1‑131 (MQ=9)

                                                                                                                         agtctgaATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGACTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAATCGCTATCTGAGTCTGAATCACTGt                   >  2:595661/1‑141 (MQ=255)

                                                                                                                            ctgaATCGCTATCTGACTCTGAGTCCCGTTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAATCGCTATCTGAGTCTGAATCACTGTCTg                <  2:401127/141‑1 (MQ=255)

                                                                                                                              gaATCGCTTTCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAATCGCTATCTGAGTCTGAATCACTGTCTGAg              <  2:780588/141‑1 (MQ=255)

                                                                                                                              gaATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAATCGCTATCTGAGTCTGAATCACTGTCTGAg              <  2:467906/141‑1 (MQ=255)

                                                                                                                                aTCGCTCTCCTAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATGTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAATCGCTATCTGAGTCTGAATCACTGTCTGAGTc            <  2:553614/141‑1 (MQ=255)

                                                                                                                                 tCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAATCGCTATCTGAGTCTGAATCACTGTCTGAGTcg           >  2:37406/1‑140 (MQ=255)

                                                                                                                                       tctgcatctgaGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAATCGCTATCTGAGTCTGAATCACTGTCTGAGTCCGAGTCa     <  1:124745/141‑1 (MQ=255)

                                                                                                                                       tctgaatctgaGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAATCGCTATCTGAGTCTGAATCACTGTCTGAGTCCGAGTCa     <  1:710225/141‑1 (MQ=255)

                                                                                                                                          gaatctgaGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAATCGCTATCTGAGTCTGAATCACTGTCTGAGTCCGAGTCACTg  <  1:637438/141‑1 (MQ=255)

GAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAATCGCTATCTGAGTCTGAATCACTGTCTGAGTCCGAGTCACTG  >  CP000730/2774913‑2775191

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`