Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 111,560 T→C 9.9% R210R (CGT→CGC USA300HOU_0110 → possible staphylococcal tandem lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730111,5600TC 9.9% 257.4 / 13.5 96R210R (CGT→CGCUSA300HOU_0110possible staphylococcal tandem lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (52/34);  new base C (5/5);  total (57/39)
Fisher's exact test for biased strand distribution p-value = 5.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

GTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATATTTACTTTACTG  >  CP000730/111425‑111699
                                                                                                                                       |                                                                                                                                           
gTTTTTTTAGAAATATAACAATTTTAAAAATTTAATGAATTATAAAGATGTAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAg                                                                                                                                        <  2:529658/141‑1 (MQ=18)
gTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAg                                                                                                                                        <  1:35260/141‑1 (MQ=255)
 ttttttGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGa                                                                                                                                       <  1:34487/141‑1 (MQ=255)
 ttttttGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATGACGTAAAAGa                                                                                                                                       >  1:142918/1‑141 (MQ=255)
     ttGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATg                                                                                                                                   <  2:714592/141‑1 (MQ=255)
     ttGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGAAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATg                                                                                                                                   <  2:422343/141‑1 (MQ=255)
      tGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATAACAATTAACTAATGATGCTTATATAGTAAAACAATTACGTAAAAGATATGa                                                                                                                                  <  2:175406/141‑1 (MQ=17)
       gcgcAATATAGCAATTTTAAAGATTTAATGAATTATGAAGATGGAGATATATCATATAAGCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGat                                                                                                                                 >  1:597884/1‑140 (MQ=17)
        cgcAATATAGCAATTTTAAATATTTATTGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAACTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACa                                                                                                                                <  1:260255/141‑1 (MQ=255)
        cgcAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGata                                                                                                                                >  2:502878/1‑139 (MQ=255)
        cgcAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAACGTAAAACAATTACGCAAAAGATATGata                                                                                                                                >  1:562863/1‑139 (MQ=17)
        cgcAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTACAACAATTACGTAAAAGATATGAct                                                                                                                                <  2:741046/141‑2 (MQ=255)
          cAATATAGCAGTTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATa                                                                                                                              >  1:593629/1‑141 (MQ=255)
          cAATATAGCAATTTTCCAGATTTAATGAATTCTAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGCCATa                                                                                                                              >  1:718331/1‑141 (MQ=255)
          cAATATAGCAATTTTAAAGATTTAGTGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATa                                                                                                                              >  1:590734/1‑141 (MQ=255)
          cAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATa                                                                                                                              <  2:204370/141‑1 (MQ=255)
          cAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATa                                                                                                                              <  2:388197/141‑1 (MQ=255)
          cAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATa                                                                                                                              >  1:661234/1‑141 (MQ=255)
          cAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAAAAATTACGTAAAAGATATGACATa                                                                                                                              >  1:472086/1‑141 (MQ=255)
          aaaTATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATa                                                                                                                              <  2:162546/140‑1 (MQ=255)
            atatCGCAAGTTTAAAGATTTAATGAATAATAAAGATGGAGATATATCAGATAGTTCAGAGGTGCCGTGTTATTCGGATAAAGATCTATTAACTAATGATGATTATAATGTAAAATATTTACGCAAAAGATATGAAATAcc                                                                                                                            <  2:9137/141‑1 (MQ=14)
            atatAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATCTAATCCAGAGGTACCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATAcc                                                                                                                            <  2:550945/141‑1 (MQ=14)
                 gCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATATTGTAAAACAATTACGTAAAAGATATGATTTACcgacga                                                                                                                       >  2:312896/1‑139 (MQ=14)
                 gCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACcgacga                                                                                                                       >  2:637612/1‑139 (MQ=11)
                 gCAACTTTAAAGCTTTAATGAATCATAAACATGGAGATATACCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACcaacaa                                                                                                                       <  1:68983/141‑1 (MQ=17)
                 acAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAAATTGTACATACcaccaa                                                                                                                       <  2:577938/140‑1 (MQ=18)
                    aTTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACcgacgagta                                                                                                                    >  2:563130/1‑136 (MQ=11)
                     ttttAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAGCAAATaa                                                                                                                   <  1:510162/141‑1 (MQ=255)
                          aaGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCAAGATATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATAACAACAAATAAAgcgc                                                                                                              >  2:593794/1‑141 (MQ=255)
                            gATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCg                                                                                                            <  2:789589/141‑1 (MQ=255)
                            gATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGATATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCACCAAATAAAccgccg                                                                                                            >  2:651130/1‑141 (MQ=255)
                              tttAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGaa                                                                                                          <  1:625902/141‑1 (MQ=255)
                                tAATGAATCATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGc                                                                                                        <  2:677131/141‑1 (MQ=255)
                                   tGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAATTATGACATACCAACAAATAAAGCGCCGAAGCtgt                                                                                                     <  1:530490/141‑1 (MQ=255)
                                   agagaaataAACATGGACATATATCATAAAATCCAGAGGTGCCGAGTTATTCAGCACAATATCAATTAACTAATGATGACTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCtgt                                                                                                     <  2:541688/135‑1 (MQ=17)
                                              gATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGt                                                                                          <  1:128841/141‑1 (MQ=255)
                                              gATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGt                                                                                          <  2:499536/141‑1 (MQ=255)
                                               aTGGAGATATATCATAAAAGCCAGAGGTGCCGAGTTATGCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTAGGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTa                                                                                         >  1:453726/1‑141 (MQ=255)
                                                tGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTAc                                                                                        <  2:67202/141‑1 (MQ=255)
                                                tGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTAc                                                                                        <  2:55012/141‑1 (MQ=255)
                                                          tCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATACATTAAATAATGATGATTATAATGTAAAACAAACAAGTATAAGATATGACATACCAACAAATAAAGCGCCGAAGCGGTTGTTGAAAGGTACAGGGAATTTa                                                                              >  2:271516/1‑141 (MQ=17)
                                                           cATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTaa                                                                             >  1:673317/1‑141 (MQ=255)
                                                                aaTCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGt                                                                        <  1:610997/141‑1 (MQ=255)
                                                                aaTCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGAGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGt                                                                        <  2:526537/141‑1 (MQ=255)
                                                                  tCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGCATTTAAAAggata                                                                      >  1:476171/1‑138 (MQ=255)
                                                                  tCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtc                                                                      >  1:461743/1‑141 (MQ=255)
                                                                  tCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtc                                                                      <  2:80950/141‑1 (MQ=255)
                                                                  tCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtc                                                                      <  2:400404/141‑1 (MQ=255)
                                                                   ccAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtca                                                                     >  1:477843/1‑141 (MQ=255)
                                                                   ccAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtca                                                                     >  1:90852/1‑141 (MQ=255)
                                                                   ccAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTGATTGAAAGGTACAGGGAATTTAAAAGGTtca                                                                     >  1:309127/1‑141 (MQ=255)
                                                                   ccAGAGGTGCCGAGTTATTCAGCGCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtca                                                                     >  2:223816/1‑141 (MQ=255)
                                                                     agagGGGCCGAGTTAGTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtcatc                                                                   >  2:677005/1‑141 (MQ=255)
                                                                         gTGCCGAGTTATTCAGCTCAGTATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGtt                                                               >  1:489675/1‑141 (MQ=255)
                                                                         gTGCCGAGTTATTCAGCTCACTATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTCTTGTTGAAGGGTACAGGGAATTTAAAAGGTTCATCAGtt                                                               >  1:507604/1‑141 (MQ=255)
                                                                         gTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATACAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGGAGTTAAAAGGGTCATCAGtt                                                               >  1:326427/1‑141 (MQ=255)
                                                                         gTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAAGGTAGAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGtt                                                               >  1:156095/1‑141 (MQ=255)
                                                                                  tATTCAACTCAATATCAATTAACTAAAGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGCTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTAGGATATaaa                                                      <  2:520917/141‑1 (MQ=255)
                                                                                      cAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATaaaaaaa                                                  >  2:712572/1‑141 (MQ=255)
                                                                                      cAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGAAATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCTTCAGTTGGatatgagatag                                                  >  2:399557/1‑134 (MQ=255)
                                                                                          tCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGa                                              >  1:702258/1‑141 (MQ=255)
                                                                                                  aaTTCACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTACATCAGTTGGATATAAAAAAATTGAATTTACtt                                      >  1:548607/1‑141 (MQ=255)
                                                                                                  aaTTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACtt                                      >  2:73294/1‑141 (MQ=255)
                                                                                                  aaTTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACtt                                      >  2:777472/1‑141 (MQ=255)
                                                                                                  aaTTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTACATCAGTTGGATATAAAAAAATTGAATTTACtt                                      >  1:548076/1‑141 (MQ=255)
                                                                                                  aaTTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACAAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTTAATTTACtt                                      >  2:138727/1‑141 (MQ=255)
                                                                                                   aTTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACttt                                     <  2:282644/141‑1 (MQ=255)
                                                                                                   aTTAAAAAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTCAAAGGTACAGGGAATTTAAAAGGTTCATCAGAAAGATAACAAAAAATTGAATTTACttt                                     <  2:453726/141‑1 (MQ=17)
                                                                                                     tAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACttttt                                   >  1:331413/1‑141 (MQ=255)
                                                                                                     tAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACttttt                                   >  1:612150/1‑141 (MQ=255)
                                                                                                     tAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACttttt                                   >  2:253407/1‑141 (MQ=255)
                                                                                                     tAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACttttt                                   >  1:612077/1‑141 (MQ=255)
                                                                                                     tAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATAAGTTGGATATAAAAAAATTGAATTTACttttt                                   >  2:188341/1‑141 (MQ=255)
                                                                                                     tAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTAAACAGTTGGATATAAAAAAATTGAATTTACttttt                                   >  2:394801/1‑141 (MQ=255)
                                                                                                       aCTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGATCATCAGTTGGATATAAAAAAATTGACTTTACTTTTTTa                                 >  2:153313/1‑141 (MQ=255)
                                                                                                         gaaTGATGATTATAATTTAAAACAATTACGTAAAAGAAATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTTAAATGTACAGTTAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTaga                               <  2:142918/140‑1 (MQ=17)
                                                                                                          aaTGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTagag                              <  1:127833/141‑1 (MQ=255)
                                                                                                          aaTGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTagag                              <  1:754393/141‑1 (MQ=255)
                                                                                                                  ttATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATaaaaa                      >  1:724757/1‑141 (MQ=255)
                                                                                                                   tataATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGATGGTTTTGAATAGATTGAATTTACTTTTTTAGAGaataataat                     >  1:221492/1‑141 (MQ=17)
                                                                                                                     taATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGa                   >  1:514754/1‑141 (MQ=255)
                                                                                                                       aTGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGaaa                 <  2:726779/141‑1 (MQ=255)
                                                                                                                       aTGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGaaa                 <  1:47373/141‑1 (MQ=255)
                                                                                                                       aTGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGaaa                 >  2:808112/1‑141 (MQ=255)
                                                                                                                             aaCAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATAttt           <  2:162134/141‑1 (MQ=255)
                                                                                                                                  ttACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATAtttacttt      >  2:434736/1‑141 (MQ=255)
                                                                                                                                  ttACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATAtttacttt      >  2:273578/1‑141 (MQ=255)
                                                                                                                                  ttACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATAtttacttta     >  2:791462/1‑141 (MQ=255)
                                                                                                                                   tACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATAtttacttta     >  1:55364/1‑141 (MQ=255)
                                                                                                                                   tACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATAtttacttta     <  1:319105/141‑1 (MQ=255)
                                                                                                                                   tACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATAtttacttta     >  1:625033/1‑141 (MQ=255)
                                                                                                                                      gTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAACGGTAAAGGGAATTAAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGACTAAGAATGACAGTATTTACTTTACTg  >  1:491707/1‑141 (MQ=18)
                                                                                                                                      gTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGTATTTACTTTTTTAGTGAATAAAAATGAAAATATTTACTTTACTg  >  2:416709/1‑141 (MQ=255)
                                                                                                                                      gTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATATTTACTTTACTg  >  1:244479/1‑141 (MQ=255)
                                                                                                                                      gTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATATTTACTTTACTg  >  1:119889/1‑141 (MQ=255)
                                                                                                                                      gTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATGGAATTTACTTTTTTAGAGAATAAAAATGAAAATATTTACTTTACTg  >  1:753632/1‑141 (MQ=255)
                                                                                                                                       |                                                                                                                                           
GTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATATTTACTTTACTG  >  CP000730/111425‑111699

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: