Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	991,415	A→T	I79F (ATT→TTT)	oppF1 →	oligopeptide ABC superfamily ATP binding cassette transporter, ABC protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	991,415	0	A	T	100.0%	46.0 / NA	15	I79F (ATT→TTT)	oppF1	oligopeptide ABC superfamily ATP binding cassette transporter, ABC protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (8/7);  total (8/7)

AAACATTAGGTTTAGTAGGAGAATCGGGGTGTGGTAAATCTACAACTGGTAAATCAATTATTAAACTTAATGATATTACAAGTGGAGAAATTTTGTATGAGGGTATTGATATACAAAAGATTCGTAAACGTAAAGATTTGCTTAAATTTAATAAAAAGATACAGATGATTTTTCAAGACCCATATGCGTCTTTAAATCCTAGGTTAAAAGTAATGGATATAGTAGCTGAAGGTATTGATATCCATCATTTAGCAACT  >  CP000730/991296‑991552                                                                                                                        |                                                                                                                                          aaaCATTATGTTTAGTAGGAGAATCGGGGTGTGGTAAATCTACAACTGGTAAATCAATTATTAAACTTAATGATATTACAAGTGGAGAAATTTTGTATGAGGGTATTGATATACAAAAGTTTCGTAAACGTAAAGATTTGc                                                                                                                      <  1:332371/141‑1 (MQ=255) aaaCATTAGGTTTAGTAGGAGAATCGGGGGGTGGTAAATCTACAACTGGTAAATCAATTATTAAACTTAATGATATTACAAGTGGAGAAATTTTGTATGAGGGTATTGATATACAAAAGTTTCGTAAACGTAAAGATTTGc                                                                                                                      <  1:106234/141‑1 (MQ=255)     aTTAGGTTTAGTAGGAGAATCGGGGTGTGGTAAATCTACAACTGGTAAATCAATTATTAAACTTAATGATATTACAAGTGGAGAAATTTTGAGGGAGGGTATTGATATACAAAAGTTTCGTAAACGTAAAGATTTGCTTaa                                                                                                                  >  1:65799/1‑141 (MQ=255)        aGGTTTAGTAGGAGAATCGGGGTGTGGTAAATCTACAACTGGTAAATCAATTATTAAACTTAATGATATTACAAGTGGAGAAATTTTGTATGAGGGTATTGATATACAAAAGTTTCGTAAACGTAAAGATTTGCTTAAAtt                                                                                                               <  1:61077/141‑1 (MQ=255)                        tCGGGGTGTGGTAAATCTACAACTGGTAAATCAAGTATTAAACTTAATGATATTACAAGTGGAGAAATTTTGTATGAGGGTATTGATATACAAAAGTTTCGTAAACGTAAAGATTTGCTTAAATTTAATAAAAAGATACAg                                                                                               <  1:280281/141‑1 (MQ=255)                                gggTAAATCTACAACTGGTAAATCAATTATTAAACTTAATGATATTACAAGTGGAGAAATTTTGTATGAGGGTATTGATATACAAAAGTTTCGTAAACGTAAAGATTTGCTTAAATTTAACAAAAAGATACAGATGAtttt                                                                                       <  1:300558/140‑1 (MQ=255)                                                  tAAATCAATTATTAAACTTAATGATATTACAACTGGAGAAATTTTGTATGAGGGTATTGATATACAAAAGTTTCGTAAACGTAAAGATTTGCTTAAATTTAATAAAAAGATACAGATGATTTTTCAAGACCCATATGCGTc                                                                     <  1:333348/141‑1 (MQ=255)                                                    aaTCAATTATTAAACTTAATGATATTACAAGTGGAGAAATTTTGTATGAGGGTATTGATATACAAAAGTTTCGTAAACGTAAAGATTTGCTTAAATTTAATAAAAAGATACAGATGATTTTTCAAGACCCATATGCGTCtt                                                                   >  1:406125/1‑141 (MQ=255)                                                          ttattaAACTTAATGATATTACAAGTGGAGAAATTTTGTATGAGGGTATTGATATACAAAAGTTTCGTAAACGTAAAGATTTGCTTAAATTTAATAAAAAGATACAGATGATTTTTCAAGACCCATATGCGTCTTTAAATc                                                             >  1:372966/1‑141 (MQ=255)                                                            attaAACTTAATGATATTACAAGTGGAGAAATTTTGTATGAGGGTATTGATATACAAAAGTTTCGTAAACGTAAAGATTTGCTTAAATTTAATAAAAAGATACAGATGATTTTTCAAGACCCATATGCGTCTTTAAATCCt                                                           >  1:34752/1‑141 (MQ=255)                                                               aaaCTTAATGATATTACAAGTGGAGAAATTTTGTATGAGGGTATTGATATACAAAAGTTTCGTAAACGTAAAGATTTGCTTAAATTTAATAAAAAGATACAGATGATTTTTCAAGACCCATATGCGTCTTTAAATCCTAgg                                                        >  1:464580/1‑141 (MQ=255)                                                                        gATATTACAAGTGGAGAAATTTTGTATGAGGGTATTGATATACAAAAGTTTCGTAAACGTAAAGATTTGCTTAAATTTAATAAAAAGATACAGATGATTTTTCAAGACCCATATGCGTCTTTAAATCCTAGGTTAAAAGTa                                               >  1:64669/1‑141 (MQ=255)                                                                                     gagaAATTTTGTATGAGGGTATTGATATACAAAAGTTTCGTAAACGTAAAGATTTGCTTAAATTTAATAAAAAGATACAGATGATTTTTCAAGACCCATATGCGTCTTTAAATCCTAGGTTAAAAGTAATGGATAtagtag                                  <  1:142660/141‑1 (MQ=255)                                                                                           ttttGTATGAGGGTATTGATATACAAAAGTTTCGTAAACGTAACGATTTGCTTAAATTTAATAAAAAGATACAGATGATTTTTCAAGACCCATATGCGTCATTAAATCCTAGTTGAAAAGTAATGGATGTAGTAGTTAAAg                            >  1:253188/1‑141 (MQ=255)                                                                                                                     aaGTTTCGTAAACGTAAAGATTTGCTTAAATTTAATAAAAAGATACAGATGATTTTTCAAGACCCATATGCGTCTTTAAATCCTAGGTTAAAAGTAATGGATATAGTAGCTGAAGGTATTGATATCCATCATTTAGCAACt  >  1:350483/1‑141 (MQ=255)                                                                                                                        |                                                                                                                                          AAACATTAGGTTTAGTAGGAGAATCGGGGTGTGGTAAATCTACAACTGGTAAATCAATTATTAAACTTAATGATATTACAAGTGGAGAAATTTTGTATGAGGGTATTGATATACAAAAGATTCGTAAACGTAAAGATTTGCTTAAATTTAATAAAAAGATACAGATGATTTTTCAAGACCCATATGCGTCTTTAAATCCTAGGTTAAAAGTAATGGATATAGTAGCTGAAGGTATTGATATCCATCATTTAGCAACT  >  CP000730/991296‑991552

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑